This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
protein
product name :
Vaccinia Virus B18R (Carrier-Free) Recombinant Protein, eBioscience
catalog :
34-8185-81
quantity :
50 ug
price :
US 814.00
citations: 11
Reference
Zorzan I, Pellegrini M, Arboit M, Incarnato D, Maldotti M, Forcato M, et al. The transcriptional regulator ZNF398 mediates pluripotency and epithelial character downstream of TGF-beta in human PSCs. Nat Commun. 2020;11:2364 pubmed publisher
Harris B, Schreiter J, Chevrier M, Jordan J, Walter M. Human interferon-ϵ and interferon-κ exhibit low potency and low affinity for cell-surface IFNAR and the poxvirus antagonist B18R. J Biol Chem. 2018;293:16057-16068 pubmed publisher
Oh S, Kessler J. Design, Assembly, Production, and Transfection of Synthetic Modified mRNA. Methods. 2018;133:29-43 pubmed publisher
van Wilgenburg B, Scherwitzl I, Hutchinson E, Leng T, Kurioka A, Kulicke C, et al. MAIT cells are activated during human viral infections. Nat Commun. 2016;7:11653 pubmed publisher
Radoshevich L, Impens F, Ribet D, Quereda J, Nam Tham T, Nahori M, et al. ISG15 counteracts Listeria monocytogenes infection. elife. 2015;4: pubmed publisher
Loh Y, Yang J, De Los Angeles A, Guo C, Cherry A, Rossi D, et al. Excision of a viral reprogramming cassette by delivery of synthetic Cre mRNA. Curr Protoc Stem Cell Biol. 2012;Chapter 4:Unit4A.5 pubmed publisher
Warren L, Manos P, Ahfeldt T, Loh Y, Li H, Lau F, et al. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell. 2010;7:618-30 pubmed publisher
Meyers J, Mangini A, Nagai T, Roff C, Sehy D, van Seventer G, et al. Blockade of TLR9 agonist-induced type I interferons promotes inflammatory cytokine IFN-gamma and IL-17 secretion by activated human PBMC. Cytokine. 2006;35:235-46 pubmed
Vancova I, La Bonnardiere C, Kontsek P. Vaccinia virus protein B18R inhibits the activity and cellular binding of the novel type interferon-delta. J Gen Virol. 1998;79 ( Pt 7):1647-9 pubmed
Symons J, Alcami A, Smith G. Vaccinia virus encodes a soluble type I interferon receptor of novel structure and broad species specificity. Cell. 1995;81:551-60 pubmed
Colamonici O, Domanski P, Sweitzer S, Larner A, Buller R. Vaccinia virus B18R gene encodes a type I interferon-binding protein that blocks interferon alpha transmembrane signaling. J Biol Chem. 1995;270:15974-8 pubmed
product information
Product Type :
Protein
Product Name :
Vaccinia Virus B18R (Carrier-Free) Recombinant Protein, eBioscience
Catalog # :
34-8185-81
Quantity :
50 ug
Price :
US 814.00
Clonality :
Recombinant
Purity :
purified
Reactivity :
Virus
Applications :
Control: Assay-Dependent, Functional Assay: Assay-Dependent
Species :
Virus
Storage :
-80 C
Description :
The B18R protein is a vaccinia virus-encoded receptor with specificity for mouse, human, rabbit, pig, rat, and cow type I interferons which has potent neutralizing activity.B18R that acts as a decoy receptor for Type I Interferons (IFNa, IFNb, IFNe,k,t,d,z, w,v). B18R was recently identified to enable increased cell viability during RNA transfection protocols designed to convert human somatic donor cells into iPSCs via direct delivery of modified synthetic mRNAs for OCT4, SOX2, KLF4 and MYC (OSKM) and Lin28 with the aim to enable highly efficient reprogramming of somatic cells to pluripotency. This allows for re-directed differentiation toward a desired lineage while removing the risk of genomic integration and insertional mutagenesis inherent to DNA-bases methodologies and eliminates the need for virus-based approaches. iPSCs represent a widely available, non-controversial and practically infinite source of pluripotent cells.The B18R protein is a type I interferon receptor encoded by the B18R gene of the Western Reserve vaccinia virus strain. The 60-65 kD glycoprotein is related to the interleukin-1 receptors and is a member of the immunoglobulin superfamily, unlike other type I IFN-receptors, which belong to the class II cytokine receptor family. The B18R protein has a high affinity (KD, 174 pM) for human IFN alpha and, unlike other type I IFN receptors, has broad species specificity, binding to type I interferons of human, mouse, rat, rabbit, pig, and cow. Among viral host response modifiers, the B18R protein is unique in that it exists as a soluble extracellular, as well as a cell surface protein, enabling blockage of both autocrine and paracrine IFN functions. The B18R protein has been shown to inhibit the antiviral potency of IFN-alpha1, IFN-alpha2, IFN-alpha-8/1/8, and IFN-omega on human cells. The soluble B18R protein is highly potent for neutralizing type I interferons, which include IFN-alpha, beta, delta, kappa. Please note effects on mouse IFNs vary from other species; it has been shown that B18R does not neutralize mouse IFN beta.
Format :
Liquid
Applications w/Dilutions :
Control: Assay-Dependent, Functional Assay: Assay-Dependent
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA