NEFM Monoclonal Antibody (RMO 14.9) | Invitrogen 34-1000 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NEFM Monoclonal Antibody (RMO 14.9)

catalog :

34-1000

quantity :

100 µg

price :

US 482

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RMO 14.9

reactivity :

human, mouse, rat, chicken, pigs , domestic sheep, domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; pigs ; 1:750; loading ...; fig 3a	Kallakuri S, Desai A, Feng K, Tummala S, Saif T, Chen C, et al. Neuronal Injury and Glial Changes Are Hallmarks of Open Field Blast Exposure in Swine Frontal Lobe. PLoS ONE. 2017;12:e0169239 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:2000; fig 5	Finnie J, Blumbergs P, Manavis J. Temporal Sequence of Autolysis in the Cerebellar Cortex of the Mouse. J Comp Pathol. 2016;154:323-8 pubmed publisher
immunohistochemistry; rat; 1 ug/ml	Zakaria N, Kallakuri S, Bandaru S, Cavanaugh J. Temporal assessment of traumatic axonal injury in the rat corpus callosum and optic chiasm. Brain Res. 2012;1467:81-90 pubmed publisher
immunohistochemistry - paraffin section; domestic sheep; fig 3	Kessell A, Finnie J, Blumbergs P, Manavis J, Jerrett I. Neuroaxonal dystrophy in Australian Merino lambs. J Comp Pathol. 2012;147:62-72 pubmed publisher
western blot; mouse; 1:1000; fig 2	Wood J, Chidlow G, Tran T, Crowston J, Casson R. A comparison of differentiation protocols for RGC-5 cells. Invest Ophthalmol Vis Sci. 2010;51:3774-83 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:500	Pal J, Toth Z, Farkas O, Kellenyi L, Doczi T, Gallyas F. Selective induction of ultrastructural (neurofilament) compaction in axons by means of a new head-injury apparatus. J Neurosci Methods. 2006;153:283-9 pubmed
immunohistochemistry - frozen section; mouse; 1:200 immunocytochemistry; mouse; 1:200	Meyer J, Katz M, Maruniak J, Kirk M. Neural differentiation of mouse embryonic stem cells in vitro and after transplantation into eyes of mutant mice with rapid retinal degeneration. Brain Res. 2004;1014:131-44 pubmed
	Tadepalli S, Bali Z, Bruszt N, Nagy L, Amrein K, Fazekas B, et al. Long-term cognitive impairment without diffuse axonal injury following repetitive mild traumatic brain injury in rats. Behav Brain Res. 2020;378:112268 pubmed publisher
	Hu Y, Li X, Huang G, Wang J, Lu W. Fasudil may induce the differentiation of bone marrow mesenchymal stem cells into neuron‑like cells via the Wnt/β‑catenin pathway. Mol Med Rep. 2019;19:3095-3104 pubmed publisher
	Samuelsson S, Lange J, Hinkle R, Tarnopolsky M, Isfort R. Corticotropin-releasing factor 2 receptor localization in skeletal muscle. J Histochem Cytochem. 2004;52:967-77 pubmed

product information

Product Type :

Antibody

Product Name :

NEFM Monoclonal Antibody (RMO 14.9)

Catalog # :

34-1000

Quantity :

100 µg

Pricing :

US 482

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Chicken, Human, Mouse, Rabbit, Rat

Applications :

ELISA: Assay-dependent, Immunocytochemistry: 1:50 - 1:100, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 0.5-2 µg/mL

Species :

Chicken, Human, Mouse, Rabbit, Rat

Clone :

RMO 14.9

Isotype :

IgG1

Storage :

-20°C

Description :

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Immunogen :

Rat NF-M.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: 1:50 - 1:100, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 0.5-2 µg/mL

Aliases :

160 kDa neurofilament protein; major neuronal intermediate filament subunit; medium neurofilament subunit; middle molecular weight neurofilament protein NF-M(2); Nef3; Nefm; nef-m; nefm.S; nefm-a; nefm-b; Neurofilament 3; neurofilament 3 (150kDa medium); neurofilament 3, medium; neurofilament M subunit; neurofilament medium; neurofilament medium polypeptide; neurofilament medium S homeolog; neurofilament medium tail domain; neurofilament protein M; Neurofilament protein, middle polypeptide; neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide S homeolog; neurofilament-3 (150 kD medium); neurofilament-M; neurofilament-M subunit; neuronal intermediate filament; NF160; NF165; NFM; NF-M; NF-M c-terminus; NF-M protein; NMC; XELAEV_18020229mg

more info or order :

Invitrogen product webpage