product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor 488
catalog :
339188
quantity :
100 ug
price :
US 558.00
clonality :
monoclonal
host :
mouse
conjugate :
AF488
clone name :
ZO1-1A12
reactivity :
human, mouse, rat, dogs
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 43
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 2.5 ug/ml
Uehara H, Zhang X, Pereira F, Narendran S, Choi S, Bhuvanagiri S, et al. Start codon disruption with CRISPR/Cas9 prevents murine Fuchs' endothelial corneal dystrophy. elife. 2021;10: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 7
Sun Y, Wu D, Zeng W, Chen Y, Guo M, Lu B, et al. The Role of Intestinal Dysbacteriosis Induced Arachidonic Acid Metabolism Disorder in Inflammaging in Atherosclerosis. Front Cell Infect Microbiol. 2021;11:618265 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 3c
Ryu H, Lee H, Lee J, Noh H, Shin M, Kumar V, et al. The molecular dynamics of subdistal appendages in multi-ciliated cells. Nat Commun. 2021;12:612 pubmed publisher
  • immunocytochemistry; human; 1:50; loading ...; fig s1g
Hempt C, Hirsch C, Hannig Y, Rippl A, Wick P, Buerki Thurnherr T. Investigating the effects of differently produced synthetic amorphous silica (E 551) on the integrity and functionality of the human intestinal barrier using an advanced in vitro co-culture model. Arch Toxicol. 2021;95:837-852 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 4c
Roy A, Murphy R, Deng M, MacDonald J, Bammler T, Aldinger K, et al. PI3K-Yap activity drives cortical gyrification and hydrocephalus in mice. elife. 2019;8: pubmed publisher
  • immunocytochemistry; human; fig 7e
Rohnalter V, Roth K, Finkernagel F, Adhikary T, Obert J, Dorzweiler K, et al. A multi-stage process including transient polyploidization and EMT precedes the emergence of chemoresistent ovarian carcinoma cells with a dedifferentiated and pro-inflammatory secretory phenotype. Oncotarget. 2015;6:40005-25 pubmed publisher
  • immunocytochemistry; dogs; 5 ug/ml
Rother J, Büchsenschütz Göbeler M, Nöding H, Steltenkamp S, Samwer K, Janshoff A. Cytoskeleton remodelling of confluent epithelial cells cultured on porous substrates. J R Soc Interface. 2015;12: pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:100; fig S3
Mok K, Mruk D, Cheng C. rpS6 regulates blood-testis barrier dynamics through Akt-mediated effects on MMP-9. J Cell Sci. 2014;127:4870-82 pubmed publisher
  • immunocytochemistry; human; 1:1000
Liu Z, Yu N, Holz F, Yang F, Stanzel B. Enhancement of retinal pigment epithelial culture characteristics and subretinal space tolerance of scaffolds with 200 nm fiber topography. Biomaterials. 2014;35:2837-50 pubmed publisher
  • immunocytochemistry; human; fig 1
Bastiaans J, van Meurs J, van Holten Neelen C, Nagtzaam N, van Hagen P, Chambers R, et al. Thrombin induces epithelial-mesenchymal transition and collagen production by retinal pigment epithelial cells via autocrine PDGF-receptor signaling. Invest Ophthalmol Vis Sci. 2013;54:8306-14 pubmed publisher
  • immunocytochemistry; human; fig 6
Warnke P, Alamein M, Skabo S, Stephens S, BOURKE R, Heiner P, et al. Primordium of an artificial Bruch's membrane made of nanofibers for engineering of retinal pigment epithelium cell monolayers. Acta Biomater. 2013;9:9414-22 pubmed publisher
  • immunocytochemistry; human; 1:1000
Liu Z, Meyer C, Fimmers R, Stanzel B. Indocyanine green concentrations used in chromovitrectomy cause a reversible functional alteration in the outer blood-retinal barrier. Acta Ophthalmol. 2014;92:e147-55 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:100
Tang E, Xiao X, Mruk D, Qian X, Mok K, Jenardhanan P, et al. Microtubule affinity-regulating kinase 4 (MARK4) is a component of the ectoplasmic specialization in the rat testis. Spermatogenesis. 2012;2:117-126 pubmed
Russell T, Dirar Q, Li Y, Chiang C, Laskowitz D, Yun Y. Cortical spheroid on perfusable microvascular network in a microfluidic device. PLoS ONE. 2023;18:e0288025 pubmed publisher
Kim E, Chen C, Gologorsky R, Santandreu A, Torres A, Wright N, et al. Feasibility of an implantable bioreactor for renal cell therapy using silicon nanopore membranes. Nat Commun. 2023;14:4890 pubmed publisher
Matsumoto A, Kitazawa T, Hatori Y, Nakanishi H, Watanabe C, Takashima T, et al. Targeting cellular gaps using Janus nanoparticles containing cationic polymers and surfactant lipids. Drug Discov Ther. 2023;17:104-113 pubmed publisher
Bodenschatz J, Ajmail K, Skamrahl M, Vache M, Gottwald J, Nehls S, et al. Epithelial cells sacrifice excess area to preserve fluidity in response to external mechanical stress. Commun Biol. 2022;5:855 pubmed publisher
Patel S, Calle Gonzalez B, Paone N, Mueller C, Floss J, Sousa M, et al. Effect of Physiological Oxygen on Primary Human Corneal Endothelial Cell Cultures. Transl Vis Sci Technol. 2022;11:33 pubmed publisher
Menck K, Heinrichs S, Wlochowitz D, Sitte M, Noeding H, Janshoff A, et al. WNT11/ROR2 signaling is associated with tumor invasion and poor survival in breast cancer. J Exp Clin Cancer Res. 2021;40:395 pubmed publisher
Miyagishima K, Sharma R, Nimmagadda M, Clore Gronenborn K, Qureshy Z, Ortolan D, et al. AMPK modulation ameliorates dominant disease phenotypes of CTRP5 variant in retinal degeneration. Commun Biol. 2021;4:1360 pubmed publisher
Lee S, Teng Y, Son M, Ku B, Hwang H, Tergaonkar V, et al. Three-Dimensional Aggregated Spheroid Model of Hepatocellular Carcinoma Using a 96-Pillar/Well Plate. Molecules. 2021;26: pubmed publisher
Pasquariello R, Verdile N, Pavlovic R, Panseri S, Schirmer K, Brevini T, et al. New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (Oncorhynchus mykiss) Retain Several Properties Observed In Vivo. Cells. 2021;10: pubmed publisher
Marquez Curtis L, Bokenfohr R, McGann L, Elliott J. Cryopreservation of human cerebral microvascular endothelial cells and astrocytes in suspension and monolayers. PLoS ONE. 2021;16:e0249814 pubmed publisher
Schneider S, De Cegli R, Nagarajan J, Kretschmer V, Matthiessen P, Intartaglia D, et al. Loss of Ciliary Gene Bbs8 Results in Physiological Defects in the Retinal Pigment Epithelium. Front Cell Dev Biol. 2021;9:607121 pubmed publisher
Paoli R, Di Giuseppe D, Badiola Mateos M, Martinelli E, Lopez Martinez M, Samitier J. Rapid Manufacturing of Multilayered Microfluidic Devices for Organ on a Chip Applications. Sensors (Basel). 2021;21: pubmed publisher
Pavelj x161 ek D, Ivi x10d ak Kocjan K, Treven P, Ben x10d ina M, Jerala R, Rogelj I. Distinctive probiotic features share common TLR2-dependent signalling in intestinal epithelial cells. Cell Microbiol. 2021;23:e13264 pubmed publisher
Huang X, Shi X, Hansen M, Setiady I, Nemeth C, Celli A, et al. Nanotopography Enhances Dynamic Remodeling of Tight Junction Proteins through Cytosolic Liquid Complexes. ACS Nano. 2020;14:13192-13202 pubmed publisher
Fang Y, Lai K, She P, Sun J, Tao W, Zhong T. Tbx20 Induction Promotes Zebrafish Heart Regeneration by Inducing Cardiomyocyte Dedifferentiation and Endocardial Expansion. Front Cell Dev Biol. 2020;8:738 pubmed publisher
Yu Y, Lu J, Oliphant K, Gupta N, Claud K, Lu L. Maternal administration of probiotics promotes gut development in mouse offsprings. PLoS ONE. 2020;15:e0237182 pubmed publisher
Kim H, Witt H, Oswald T, Tarantola M. Adhesion of Epithelial Cells to PNIPAm Treated Surfaces for Temperature-Controlled Cell-Sheet Harvesting. ACS Appl Mater Interfaces. 2020;12:33516-33529 pubmed publisher
Hempt C, Kaiser J, Scholder O, Buerki Thurnherr T, Hofmann H, Rippl A, et al. The impact of synthetic amorphous silica (E 551) on differentiated Caco-2 cells, a model for the human intestinal epithelium. Toxicol In Vitro. 2020;67:104903 pubmed publisher
Zheng L, Wang J, Xu J, Zhang X, Liu B, Huang L, et al. Magnesium and vitamin C supplementation attenuates steroid-associated osteonecrosis in a rat model. Biomaterials. 2020;238:119828 pubmed publisher
Lux T, Hu X, Ben Kraiem A, Blum R, Chen J, Rittner H. Regional Differences in Tight Junction Protein Expression in the Blood-DRG Barrier and Their Alterations after Nerve Traumatic Injury in Rats. Int J Mol Sci. 2019;21: pubmed publisher
Samy K, Levy E, Phong K, Demaree B, Abate A, Desai T. Human intestinal spheroids cultured using Sacrificial Micromolding as a model system for studying drug transport. Sci Rep. 2019;9:9936 pubmed publisher
Sherman H, Rossi A. A Novel Three-Dimensional Glioma Blood-Brain Barrier Model for High-Throughput Testing of Tumoricidal Capability. Front Oncol. 2019;9:351 pubmed publisher
Freire R, Ingano L, Serena G, Cetinbaş M, Anselmo A, Sapone A, et al. Human gut derived-organoids provide model to study gluten response and effects of microbiota-derived molecules in celiac disease. Sci Rep. 2019;9:7029 pubmed publisher
Schneider S, Hotaling N, Campos M, Patnaik S, Bharti K, May Simera H. Generation of an inducible RPE-specific Cre transgenic-mouse line. PLoS ONE. 2018;13:e0207222 pubmed publisher
Zhang C, Lee H, Shrivastava A, Wang R, McQuiston T, Challberg S, et al. Long-Term In Vitro Expansion of Epithelial Stem Cells Enabled by Pharmacological Inhibition of PAK1-ROCK-Myosin II and TGF-β Signaling. Cell Rep. 2018;25:598-610.e5 pubmed publisher
Toosi B, El Zawily A, Truitt L, Shannon M, Allonby O, Babu M, et al. EPHB6 augments both development and drug sensitivity of triple-negative breast cancer tumours. Oncogene. 2018;37:4073-4093 pubmed publisher
Xu J, Zhang L, Ye Y, Shan Y, Wan C, Wang J, et al. SNX16 Regulates the Recycling of E-Cadherin through a Unique Mechanism of Coordinated Membrane and Cargo Binding. Structure. 2017;25:1251-1263.e5 pubmed publisher
Marneros A. NLRP3 inflammasome blockade inhibits VEGF-A-induced age-related macular degeneration. Cell Rep. 2013;4:945-58 pubmed publisher
Chambrey R, Kurth I, Peti Peterdi J, Houillier P, Purkerson J, Leviel F, et al. Renal intercalated cells are rather energized by a proton than a sodium pump. Proc Natl Acad Sci U S A. 2013;110:7928-33 pubmed publisher
Mok K, Mruk D, Silvestrini B, Cheng C. rpS6 Regulates blood-testis barrier dynamics by affecting F-actin organization and protein recruitment. Endocrinology. 2012;153:5036-48 pubmed
product information
Product Type :
Antibody
Product Name :
ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor 488
Catalog # :
339188
Quantity :
100 ug
Price :
US 558.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Canine, Human
Applications :
ELISA: 0.1-1.0 ug/mL (unconjugated), Immunocytochemistry: 5 ug/mL, Western Blot: 1-2 ug/mL
Species :
Canine, Human
Clone :
ZO1-1A12
Isotype :
IgG1
Storage :
4 C, store in dark
Description :
ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
Immunogen :
Human recombinant ZO-1 fusion protein encompassing amino acids 334-634
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-1.0 ug/mL (unconjugated), Immunocytochemistry: 5 ug/mL, Western Blot: 1-2 ug/mL
Aliases :
DKFZp686M05161; I79_005485; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); tight junction protein ZO-1; Tjp1; zo1; ZO-1; ZO-1 MDCK; zona occludens 1; zona occludens protein 1; zonula occludens 1; zonula occludens 1 protein; zonula occludens protein 1
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA