Histone H3 Polyclonal Antibody
Invitrogen
catalog: PA5-16183
domestic rabbit polyclonal
reactivity: Chlamydomonas reinhardtii, human, house mouse, brown rat, bantam, bovine, leopard danio, Drosophila melanogaster
application: western blot, immunocytochemistry
citations: 3
quantity: 100µL
price: US 345
to the supplier
Phospho-Histone H3 (Ser10) Polyclonal Antibody
Invitrogen
catalog: PA5-17869
domestic rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288C, human, house mouse, brown rat, Drosophila melanogaster, Xenopus laevis
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 4

Immunofluorescent analysis of Phospho-Histone H3 pSer10 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or a ToxInsight Instrument at 20X magnification.

Immunohistochemistry was performed on paraffin-embedded human breast carcinoma. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes at 95C. Following antigen-retrieval, tissues were incubated in 3% hydrogen peroxide for 10 minutes, washed, and then blocked in 5% normal goat serum in TBST for 1 hour at room temperature. Tissues were stained with a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:200 overnight at 4C. Tissues were washed extensively with TBS + 0.1% Tween-20 and detection was performed using a HRP-conjugated secondary reagent followed by DAB substrate.

Flow cytometric analysis of Phospho-Histone H3 pSer10 on Ramos cells left untreated (blue histogram) or treated with serum/calyculin (green histogram). Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-Histone H3 pSer10 polyclonal antibody (Product # PA5-17869) at a dilution of 1:50 for 1 hour at room temperature. As a negative control, serum/calyculin-treated cells were stained with an isotype control (red histogram). Following staining with either PA5-17869 or isotype control, cells were stained with a fluorescently-conjugated anti-rabbit IgG secondary antibody and analyzed on a flow cytometer.
quantity: 100µL
price: US 345
to the supplier
Histone H3 Monoclonal Antibody (E.960.2)
Invitrogen
catalog: MA5-15150
domestic rabbit monoclonal (E.960.2)
reactivity: human, house mouse, brown rat, dogs, bovine, swine, leopard danio, Drosophila melanogaster
application: western blot
citations: 3
quantity: 100µL
price: US 345
to the supplier
Tri-Methyl-Histone H3 (Lys4) Monoclonal Antibody (G.532.8)
Invitrogen
catalog: MA5-11199
domestic rabbit monoclonal (G.532.8)
reactivity: Saccharomyces cerevisiae S288C, human, house mouse, brown rat, leopard danio, Drosophila melanogaster, Xenopus laevis
application: western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 1
quantity: 100µL
price: US 345
to the supplier
Phospho-Acetyl-Histone H3 (Ser10, Lys9) Monoclonal Antibody (APH3-64)
Invitrogen
catalog: MA1-25461
house mouse monoclonal (APH3-64)
reactivity: human, house mouse, brown rat, Drosophila melanogaster
modification: phosphorylated
application: western blot, ELISA, immunoprecipitation
quantity: 50µL
price: US 345
to the supplier
Di-Methyl-Histone H3 (Lys9) Polyclonal Antibody
Invitrogen
catalog: PA5-16195
domestic rabbit polyclonal
reactivity: human, house mouse, brown rat, Drosophila melanogaster
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
quantity: 100µL
price: US 345
to the supplier
Phospho-Histone H3 (Ser28) Polyclonal Antibody
Invitrogen
catalog: PA5-17318
domestic rabbit polyclonal
reactivity: human, house mouse, brown rat, bantam, bovine, leopard danio, Drosophila melanogaster, Xenopus laevis
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
quantity: 100µL
price: US 345
to the supplier
Histone H3.3 Polyclonal Antibody
Invitrogen
catalog: PA5-23288
domestic rabbit polyclonal
reactivity: human, house mouse, bantam, bovine, Drosophila melanogaster, domestic horse
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section


quantity: 100µg
price: US 345
to the supplier