Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 488 | Invitrogen 331588 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 488

catalog :

331588

quantity :

100 µg

price :

US 428

clonality :

monoclonal

host :

mouse

conjugate :

AF488

clone name :

OC-3F10

reactivity :

human, mouse, rat, dogs

application :

western blot, ELISA, immunocytochemistry, immunohistochemistry - frozen section, immunohistochemistry - free floating section

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - free floating section; mouse; 1:400; loading ...; fig s4a	Yanagida K, Liu C, Faraco G, Galvani S, Smith H, Burg N, et al. Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1. Proc Natl Acad Sci U S A. 2017;114:4531-4536 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; fig s8	Campos Y, Qiu X, Gomero E, Wakefield R, Horner L, Brutkowski W, et al. Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier. Nat Commun. 2016;7:11876 pubmed publisher
immunocytochemistry; human; fig 2	Rodriguez M, Kaushik A, Lapierre J, Dever S, El Hage N, Nair M. Electro-Magnetic Nano-Particle Bound Beclin1 siRNA Crosses the Blood-Brain Barrier to Attenuate the Inflammatory Effects of HIV-1 Infection in Vitro. J Neuroimmune Pharmacol. 2017;12:120-132 pubmed publisher
immunocytochemistry; human; fig 4	Blume C, Reale R, Held M, Millar T, Collins J, Davies D, et al. Temporal Monitoring of Differentiated Human Airway Epithelial Cells Using Microfluidics. PLoS ONE. 2015;10:e0139872 pubmed publisher
	Munis A, Hyde S, Gill D. A human surfactant B deficiency air-liquid interface cell culture model suitable for gene therapy applications. Mol Ther Methods Clin Dev. 2021;20:237-246 pubmed publisher
	Jing B, Wang Z, Zhang C, Deng Q, Wei J, Luo Y, et al. Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction. Front Bioeng Biotechnol. 2020;8:272 pubmed publisher
	Sherman H, Rossi A. A Novel Three-Dimensional Glioma Blood-Brain Barrier Model for High-Throughput Testing of Tumoricidal Capability. Front Oncol. 2019;9:351 pubmed publisher
	DaSilva Arnold S, Kuo C, Davra V, Remache Y, Kim P, Fisher J, et al. ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation. Mol Hum Reprod. 2019;25:61-75 pubmed publisher
	Li Y, Bai Y, Jiang Y, Yu W, Shi X, Chen L, et al. Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3K/Akt and MAPK/Erk Signaling Pathways. Biomed Res Int. 2018;2018:3242574 pubmed publisher
	Alhouayek M, Gouveia Figueira S, Hammarström M, Fowler C. Involvement of CYP1B1 in interferon ?-induced alterations of epithelial barrier integrity. Br J Pharmacol. 2017;: pubmed publisher
	Ramos C, Lin C, Liu X, Antonetti D. The EPAC-Rap1 pathway prevents and reverses cytokine-induced retinal vascular permeability. J Biol Chem. 2018;293:717-730 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 488

Catalog # :

331588

Quantity :

100 µg

Pricing :

US 428

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Rat

Applications :

ELISA: Assay-dependent, Immunocytochemistry: 1-5 µg/mL, Western Blot: Assay-dependent

Species :

Canine, Human, Mouse, Rat

Clone :

OC-3F10

Isotype :

IgG1, kappa

Storage :

4° C, store in dark

Description :

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

Immunogen :

GST-fusion protein consisting of the C-terminal region (~150 aa) of human occludin.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: 1-5 µg/mL, Western Blot: Assay-dependent

Aliases :

AI503564; BLCPMG; Occludin; occludin 1B; Ocl; Ocln; phosphatase 1, regulatory subunit 115; PPP1R115; tight junction protein occludin; tight junction structural protein

more info or order :

Invitrogen product webpage