domestic rabbit polyclonal
reactivity: human, rat
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of Phospho Heat Shock Protein 27 (p-HSP27) pSer15 was performed by loading 50ug of HeLa cell lysates from untreated cells (left lane) or cells treated with 10uM Anisomycin (right lane) for 30 minutes and 15ul PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-016) at a dilution of 1:500 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

Immunoprecipitation of Phospho Heat Shock Protein (p-HSP27) pSer15 was performed on HeLa cells treated with 10uM Anisomysin for 30 minutes. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 3ug of p-HSP27 pSer15 polyclonal antibody (Product # PA1-016) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with Lane Marker Reducing Sample Buffer (Product # 39000). HeLa cell lysate (50ug) was loaded as a positive control (left lane). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-016) at a dilution of 1:500 overnight rotating at 4°C, washed in TBST, and probed with Clean-Blot IP Detection Reagent (Product # 21230) at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

Immunofluorescent analysis of Phospho-HSP27 (p-HSP27) pSer15 (green) in HeLa cells either left untreated (left panel) or treated with 10uM Anisomysin (right panel) for 30 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-016), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
quantity: 100 ug
price: US 419.00
to the supplier
mouse monoclonal (STRSN)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 4
quantity: 25 ug
price: US 156.00
to the supplier
HSP27 Polyclonal Antibody
Invitrogen
catalog: PA1-017
domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2
quantity: 100 ug
price: US 491.00
to the supplier
domestic rabbit polyclonal
reactivity: human, rat
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 1

Immunoprecipitation of Phospho Heat Shock Protein 27 (p-HSP27) pSer15 was performed on HeLa cells treated with 10uM Anisomysin for 30 minutes. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 3ug of p-HSP27 pSer15 polyclonal antibody (Product # PA1-018) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with Lane Marker Reducing Sample Buffer (Product # 39000). Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-018) at a dilution of 1:500 overnight rotating at 4°C, washed with TBST, and probed with Clean-Blot IP Detection Reagent (Product # 21230) at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

Immunofluorescent analysis of Phospho Heat Shock Protein 27 (p-HSP27) pSer15 (green) in HeLa cells either left untreated (left panel) or treated with 10uM Anisomysin (right panel) for 30 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-018) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Western blot analysis of Phospho Heat Shock Protein 27 (p-HSP27) pSer15 was performed by loading 50ug of HeLa cell lysates from untreated cells (left lane) or cells treated with 10uM Anisomycin (right lane) for 30 minutes and 15ul PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-018) at a dilution of 1:500 overnight at 4°C on a rocking platform, washed with TBS-0.1%Tween-20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
quantity: 100 ug
price: US 520.00
to the supplier
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1
quantity: 100 uL
price: US 485.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse
modification: phosphorylated
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 520.00
to the supplier
domestic rabbit recombinant (10C11)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 1
quantity: 100 uL
price: US 592.00
to the supplier
Phospho-HSP27 (Ser78) Polyclonal Antibody
Invitrogen
catalog: PA5-17662
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Immunofluorescent analysis of Phospho Heat Shock Protein 27 (p-HSP27) pSer78 (green) in HeLa cells either left untreated (left panel) or treated with 25ug/ml anisomycin for 30 min (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-HSP27 pSer78 polyclonal antibody (Product # PA5-17662) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.

Immunohistochemistry was performed on paraffin-embedded human breast carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes at 95C. Following antigen-retrieval, tissues were incubated in 3% hydrogen peroxide for 10 minutes, washed, and then blocked in 5% normal goat serum in TBST for 1 hour at room temperature. Tissues were stained with a phospho-HSP27 pSer78 polyclonal antibody (Product # PA5-17662) at a dilution of 1:100 overnight at 4C. Tissues were washed extensively with TBS + 0.1% Tween-20 and detection was performed using an HRP-conjugated secondary reagent followed by DAB substrate.
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-HSP27 (Ser78) Polyclonal Antibody
Invitrogen
catalog: PA5-12779
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, dot blot

quantity: 400 uL
price: US 464.00
to the supplier
HSP27 Polyclonal Antibody
Invitrogen
catalog: AHO1132
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 ug
price: US 500.00
to the supplier
Phospho-HSP27 (Ser78) Polyclonal Antibody
Invitrogen
catalog: PA5-23340
domestic rabbit polyclonal
reactivity: human, dogs, domestic horse
modification: phosphorylated
application: western blot
quantity: 20 ug
price: US 464.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
HSP27 Polyclonal Antibody
Invitrogen
catalog: PA5-78010
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 514.00
to the supplier
domestic rabbit recombinant (155)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
quantity: 100 uL
price: US 474.00
to the supplier
domestic rabbit recombinant (SN06-73)
reactivity: human, mouse, rat
application: western blot
quantity: 100 uL
price: US 474.00
to the supplier
domestic rabbit recombinant (JJ09-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 530.00
to the supplier
HSP27 Polyclonal Antibody
Invitrogen
catalog: PA5-85351
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 454.00
to the supplier
domestic rabbit recombinant (11H29L1)
reactivity: human
application: western blot
quantity: 100 ug
price: US 500.00
to the supplier
Phospho-HSP27 (Ser82) Polyclonal Antibody
Invitrogen
catalog: PA5-97340
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit recombinant (2.00E+08)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 491.00
to the supplier
domestic rabbit recombinant (2D8)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 491.00
to the supplier
domestic rabbit recombinant (ARC1576)
reactivity: human, rat
application: western blot, ELISA
quantity: 100 uL
price: US 491.00
to the supplier
domestic rabbit recombinant (ARC1577)
reactivity: human, mouse
application: western blot, ELISA
quantity: 100 uL
price: US 491.00
to the supplier
domestic rabbit recombinant (ARC0531)
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry
quantity: 100 uL
price: US 464.00
to the supplier
mouse monoclonal (GT357)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 444.00
to the supplier
domestic rabbit recombinant (HSP27S82-CB2)
reactivity: human, mouse
application: western blot, flow cytometry
quantity: 200 uL
price: US 626.00
to the supplier
domestic rabbit recombinant (HSP27S82-CB2)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
quantity: 100 Tests
price: US 484.00
to the supplier
domestic rabbit recombinant (HSP27S82-CB2)
reactivity: human, mouse
conjugate: FITC
application: flow cytometry
quantity: 100 Tests
price: US 474.00
to the supplier
domestic rabbit recombinant (HSP27S82-CB2)
reactivity: human, mouse
conjugate: APC
application: flow cytometry
quantity: 100 Tests
price: US 474.00
to the supplier
domestic rabbit recombinant (HSP27S82-CB2)
reactivity: human, mouse
application: western blot, flow cytometry
quantity: 20 uL
price: US 329.00
to the supplier
domestic rabbit recombinant (HSP27S82-CB2)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
quantity: 10 Tests
price: US 197.00
to the supplier
domestic rabbit recombinant (HSP27S82-CB2)
reactivity: human, mouse
conjugate: FITC
application: flow cytometry
quantity: 10 Tests
price: US 197.00
to the supplier
domestic rabbit recombinant (HSP27S82-CB2)
reactivity: human, mouse
conjugate: APC
application: flow cytometry
quantity: 10 Tests
price: US 197.00
to the supplier
domestic rabbit recombinant (155)
reactivity: human
conjugate: FITC
application: flow cytometry
quantity: 100 Tests
price: US 489.00
to the supplier
domestic rabbit recombinant (155)
reactivity: human
conjugate: PE
application: flow cytometry
quantity: 100 Tests
price: US 489.00
to the supplier
domestic rabbit recombinant (17)
reactivity: human
application: western blot, ELISA, immunoprecipitation
quantity: 100 uL
price: US 444.00
to the supplier
domestic rabbit recombinant (JJ08-70)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
HSP27 Monoclonal Antibody (10-8)
Invitrogen
catalog: MA5-44616
mouse monoclonal (8-Oct)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
HSP27 Monoclonal Antibody (8A7), APC
Invitrogen
catalog: MA5-45121
mouse monoclonal (8A7)
reactivity: Chinese hamsters, guinea pig, human, mouse, rat, dogs, bovine, domestic sheep
conjugate: APC
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 410.00
to the supplier
HSP27 Monoclonal Antibody (8A7), FITC
Invitrogen
catalog: MA5-45122
mouse monoclonal (8A7)
reactivity: Chinese hamsters, guinea pig, human, mouse, rat, dogs, bovine, domestic sheep
conjugate: FITC
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 410.00
to the supplier
HSP27 Monoclonal Antibody (8A7), PerCP
Invitrogen
catalog: MA5-45123
mouse monoclonal (8A7)
reactivity: Chinese hamsters, guinea pig, human, mouse, rat, dogs, bovine, domestic sheep
conjugate: PerCP
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 410.00
to the supplier
HSP27 Monoclonal Antibody (5D12-A12), APC
Invitrogen
catalog: MA5-45205
mouse monoclonal (5D12-A12)
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
quantity: 200 ug
price: US 444.00
to the supplier
HSP27 Monoclonal Antibody (5D12-A12), FITC
Invitrogen
catalog: MA5-45206
mouse monoclonal (5D12-A12)
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
quantity: 200 ug
price: US 410.00
to the supplier
mouse monoclonal (5D12-A12)
reactivity: human
conjugate: PerCP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
quantity: 200 ug
price: US 444.00
to the supplier
HSP27 Monoclonal Antibody (6H11)
Invitrogen
catalog: MA5-47365
mouse monoclonal (6H11)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity: 500 uL
price: US 1648.00
to the supplier
HSP27 Monoclonal Antibody (6H11)
Invitrogen
catalog: MA5-47464
mouse monoclonal (6H11)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity: 100 uL
price: US 480.00
to the supplier
domestic rabbit recombinant (SAIC-23B-22)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
quantity: 200 ug
price: US 520.00
to the supplier
domestic rabbit recombinant (SAIC-23C-35)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
quantity: 200 ug
price: US 520.00
to the supplier
domestic rabbit recombinant (SAIC-23E-30)
reactivity: human
application: immunocytochemistry
quantity: 200 ug
price: US 520.00
to the supplier
HSP27 Monoclonal Antibody (C1)
Invitrogen
catalog: MA5-50979
mouse monoclonal (C1)
reactivity: human, pigs
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 200 uL
price: US 393.00
to the supplier
HSP27 Monoclonal Antibody (C1)
Invitrogen
catalog: MA5-50980
mouse monoclonal (C1)
reactivity: human, pigs
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 20 uL
price: US 181.00
to the supplier
Phospho-HSP27 (Ser78) Polyclonal Antibody
Invitrogen
catalog: PA5-114583
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-HSP27 (Ser78) Polyclonal Antibody
Invitrogen
catalog: PA5-117187
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry
quantity: 100 uL
price: US 474.00
to the supplier
HSP27 Polyclonal Antibody
Invitrogen
catalog: PA5-119177
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 200 uL
price: US 410.00
to the supplier
HSP27 Polyclonal Antibody
Invitrogen
catalog: PA5-121898
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 20 uL
price: US 151.00
to the supplier
HSP27 Polyclonal Antibody
Invitrogen
catalog: PA5-143536
chicken polyclonal
reactivity: human, rat
application: western blot, immunocytochemistry
quantity: 500 uL
price: US 1614.00
to the supplier
HSP27 Polyclonal Antibody
Invitrogen
catalog: PA5-143570
chicken polyclonal
reactivity: human, rat
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 470.00
to the supplier