NFkB p65 Monoclonal Antibody (2A12A7) | Invitrogen 33-9900 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NFkB p65 Monoclonal Antibody (2A12A7)

catalog :

33-9900

quantity :

100 µg

price :

US 482

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2A12A7

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - free floating section

more info or order :

Invitrogen product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 6a	Niu Q, Liu Z, Alamer E, Fan X, Chen H, Endsley J, et al. Structure-guided drug design identifies a BRD4-selective small molecule that suppresses HIV. J Clin Invest. 2019;129:3361-3373 pubmed publisher
western blot; human	Lee Y, Wang E, Kumar N, Glickman R. Ursolic acid differentially modulates apoptosis in skin melanoma and retinal pigment epithelial cells exposed to UV-VIS broadband radiation. Apoptosis. 2014;19:816-28 pubmed publisher
immunohistochemistry - free floating section; mouse; fig 6 immunocytochemistry; mouse; fig 5 western blot; mouse; fig 1	Herkenham M, Rathore P, Brown P, Listwak S. Cautionary notes on the use of NF-?B p65 and p50 antibodies for CNS studies. J Neuroinflammation. 2011;8:141 pubmed publisher
western blot; human; fig 4	Chen M, Tsai T, Lin Y, Tsai Y, Wang L, Lee M, et al. Antipsychotic drugs suppress the AKT/NF-?B pathway and regulate the differentiation of T-cell subsets. Immunol Lett. 2011;140:81-91 pubmed publisher
immunocytochemistry; human; fig 1	Jagla B, Aulner N, Kelly P, Song D, Volchuk A, Zatorski A, et al. Sequence characteristics of functional siRNAs. RNA. 2005;11:864-72 pubmed
	Indukuri R, Hases L, Archer A, Williams C. Estrogen Receptor Beta Influences the Inflammatory p65 Cistrome in Colon Cancer Cells. Front Endocrinol (Lausanne). 2021;12:650625 pubmed publisher
	Krajewski A, Gagat M, Mikołajczyk K, Izdebska M, Zuryn A, Grzanka A. Cyclin F Downregulation Affects Epithelial-Mesenchymal Transition Increasing Proliferation and Migration of the A-375 Melanoma Cell Line. Cancer Manag Res. 2020;12:13085-13097 pubmed publisher
	Alamer E, Zhong C, Liu Z, Niu Q, Long F, Guo L, et al. Epigenetic Suppression of HIV in Myeloid Cells by the BRD4-Selective Small Molecule Modulator ZL0580. J Virol. 2020;94: pubmed publisher
	Mejhert N, Kuruvilla L, Gabriel K, Elliott S, Guie M, Wang H, et al. Partitioning of MLX-Family Transcription Factors to Lipid Droplets Regulates Metabolic Gene Expression. Mol Cell. 2020;77:1251-1264.e9 pubmed publisher
	Gisbert Ferrándiz L, Salvador P, Ortiz Masia D, Macías Ceja D, Orden S, Esplugues J, et al. A Single Nucleotide Polymorphism in the Vitamin D Receptor Gene Is Associated With Decreased Levels of the Protein and a Penetrating Pattern in Crohn's Disease. Inflamm Bowel Dis. 2018;24:1462-1470 pubmed publisher
	Alegre F, Moragrega A, Polo M, Marti Rodrigo A, Esplugues J, Blas García A, et al. Role of p62/SQSTM1 beyond autophagy: a lesson learned from drug-induced toxicity in vitro. Br J Pharmacol. 2017;: pubmed publisher
	McDonald K, Cooper S, Danielzak L, Leask R. Glycocalyx Degradation Induces a Proinflammatory Phenotype and Increased Leukocyte Adhesion in Cultured Endothelial Cells under Flow. PLoS ONE. 2016;11:e0167576 pubmed publisher

product information

Product Type :

Antibody

Product Name :

NFkB p65 Monoclonal Antibody (2A12A7)

Catalog # :

33-9900

Quantity :

100 µg

Pricing :

US 482

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

ChIP assay: 3 µg/mL, ELISA: Assay-dependent, Gel Shift: Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 0.5 - 1 µg/mL

Species :

Human, Mouse

Clone :

2A12A7

Isotype :

IgG2a

Storage :

-20°C

Description :

Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

Immunogen :

Recombinant protein (~175 amino acids) from the C-terminal portion of human NF-kappa-B (p65).

Format :

Liquid

Applications w/Dilutions :

ChIP assay: 3 µg/mL, ELISA: Assay-dependent, Gel Shift: Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 0.5 - 1 µg/mL

Aliases :

avian reticuloendotheliosis viral (v-rel) oncogene homolog A; EBP-1; I79_021812; MGC131774; NF KB; NFkappaB p65; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NF-kappaB transcription factor p65 subunit; NFkB; NF-kB p65 subunit; nfkb rela; NFKB1; nfkb3; NFkB-p50; nuclear factor; nuclear factor kappa b; nuclear factor kappa B subunit p65; nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; P65 transcription factor; putative transcription factor p65 homolog; REL A; rel1; rela; RELA proto-oncogene, NF-kB subunit; rela.L; rela-a; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel avian reticuloendotheliosis viral oncogene homolog A L homeolog; v-rel reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65; XELAEV_18022301mg; Xrel1; xrela

more info or order :

Invitrogen product webpage