SR Monoclonal Antibody (16H3 (16H3E8)) | Invitrogen 33-9300 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

SR Monoclonal Antibody (16H3 (16H3E8))

catalog :

33-9300

quantity :

100 µg

price :

US 428

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

16H3 (16H3E8)

reactivity :

human, mouse, rat, dogs, chicken, bovine, domestic rabbit

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:200; fig 3	Kaehler C, Guenther A, Uhlich A, Krobitsch S. PRMT1-mediated arginine methylation controls ATXN2L localization. Exp Cell Res. 2015;334:114-25 pubmed publisher
western blot; human; fig 1	Katahira J, Okuzaki D, Inoue H, Yoneda Y, Maehara K, Ohkawa Y. Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I. Nucleic Acids Res. 2013;41:7060-72 pubmed publisher
western blot; human; fig 1	Vivarelli S, Lenzken S, Ruepp M, Ranzini F, Maffioletti A, Alvarez R, et al. Paraquat modulates alternative pre-mRNA splicing by modifying the intracellular distribution of SRPK2. PLoS ONE. 2013;8:e61980 pubmed publisher
western blot; rat; fig 3	Walsh C, Suchanek A, Cyphert T, Kohan A, Szeszel Fedorowicz W, Salati L. Serine arginine splicing factor 3 is involved in enhanced splicing of glucose-6-phosphate dehydrogenase RNA in response to nutrients and hormones in liver. J Biol Chem. 2013;288:2816-28 pubmed publisher
western blot; mouse; 1:100; fig 3 western blot; human; 1:100; fig 3	Toiber D, Azkona G, Ben Ari S, Toran N, Soreq H, Dierssen M. Engineering DYRK1A overdosage yields Down syndrome-characteristic cortical splicing aberrations. Neurobiol Dis. 2010;40:348-59 pubmed publisher
western blot; human; fig 1	Chandradas S, Deikus G, Tardos J, Bogdanov V. Antagonistic roles of four SR proteins in the biosynthesis of alternatively spliced tissue factor transcripts in monocytic cells. J Leukoc Biol. 2010;87:147-52 pubmed publisher
western blot; human; fig 3	Chen Y, Yuo C, Yang W, Jong Y, Lin H, Chang Y, et al. Extracellular pH change modulates the exon 7 splicing in SMN2 mRNA. Mol Cell Neurosci. 2008;39:268-72 pubmed publisher
western blot; mouse; fig 2 western blot; human; 1:2000; fig 1	Yomoda J, Muraki M, Kataoka N, Hosoya T, Suzuki M, Hagiwara M, et al. Combination of Clk family kinase and SRp75 modulates alternative splicing of Adenovirus E1A. Genes Cells. 2008;13:233-44 pubmed publisher
immunoprecipitation; human; fig 4	Exline C, Feng Z, Stoltzfus C. Negative and positive mRNA splicing elements act competitively to regulate human immunodeficiency virus type 1 vif gene expression. J Virol. 2008;82:3921-31 pubmed publisher
western blot; human; fig 2	Liang W, Yuo C, Chang J, Chen Y, Chang Y, Wang H, et al. The effect of hydroxyurea in spinal muscular atrophy cells and patients. J Neurol Sci. 2008;268:87-94 pubmed publisher
western blot; human; 1:500	Yuo C, Lin H, Chang Y, Yang W, Chang J. 5-(N-ethyl-N-isopropyl)-amiloride enhances SMN2 exon 7 inclusion and protein expression in spinal muscular atrophy cells. Ann Neurol. 2008;63:26-34 pubmed
western blot; human; 1:1000	Huang Y, Kim Y, Li Z, Fomenkov T, Fomenkov A, Ratovitski E. AEC-associated p63 mutations lead to alternative splicing/protein stabilization of p63 and modulation of Notch signaling. Cell Cycle. 2005;4:1440-7 pubmed
western blot; mouse	Chang J, Hsieh Li H, Jong Y, Wang N, Tsai C, Li H. Treatment of spinal muscular atrophy by sodium butyrate. Proc Natl Acad Sci U S A. 2001;98:9808-13 pubmed
	Damodaran A, Courtheoux T, Watrin E, Prigent C. Alteration of SC35 localization by transfection reagents. Biochim Biophys Acta Mol Cell Res. 2020;1867:118650 pubmed publisher
	Martínez Pizarro A, Dembic M, Perez B, Andresen B, Desviat L. Intronic PAH gene mutations cause a splicing defect by a novel mechanism involving U1snRNP binding downstream of the 5' splice site. PLoS Genet. 2018;14:e1007360 pubmed publisher
	Tyson Capper A, Bailey J, Krainer A, Robson S, Europe Finner G. The switch in alternative splicing of cyclic AMP-response element modulator protein CREM{tau}2{alpha} (activator) to CREM{alpha} (repressor) in human myometrial cells is mediated by SRp40. J Biol Chem. 2005;280:34521-9 pubmed

product information

Product Type :

Antibody

Product Name :

SR Monoclonal Antibody (16H3 (16H3E8))

Catalog # :

33-9300

Quantity :

100 µg

Pricing :

US 428

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bovine, Canine, Chicken, Drosophila, Human, Mouse, Rabbit, Rat, Xenopus laevis

Applications :

ELISA: 0.1-1 µg/mL, Immunocytochemistry: 0.5-2 µg/mL, Western Blot: 3-5 µg/mL

Species :

Bovine, Canine, Chicken, Drosophila, Human, Mouse, Rabbit, Rat, Xenopus laevis

Clone :

16H3 (16H3E8)

Isotype :

IgG1, kappa

Storage :

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Description :

SR proteins are a highly conserved family of arginine/serine-rich, spliceosome-associated phosphoproteins essential for metazoan pre-mRNA splicing. SR proteins act early in splicing by promoting splice site recognition and spliceosome assembly. SR proteins also play a regulatory role because they can determine alternative splice site usage in vivo and in vitro. SR proteins appear to be recruited from nuclear “speckles”, in which they are concentrated to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus.

Immunogen :

Purified, de-phosphorylated bovine Srp55 and Drosophila p55-GST. The 16H3 epitope has been mapped to a 40-amino acid polypeptide composed almost exclusively of arginine alternating with glutamate and aspartate

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.1-1 µg/mL, Immunocytochemistry: 0.5-2 µg/mL, Western Blot: 3-5 µg/mL

Aliases :

1210001E11Rik; 5730499P16Rik; AI314910; AL024116; AW146126; AW550192; B52; Cl-4; Delayed-early protein HRS; epididymis secretory protein Li 91; HEL-S-91; HRS; hypothetical protein; insulin-induced growth response protein CL-4; LOW QUALITY PROTEIN: serine/arginine-rich splicing factor 4; MNCb-2616; pre-mRNA splicing factor SRp20; pre-mRNA-splicing factor SRP20; Pre-mRNA-splicing factor SRP40; pre-mRNA-splicing factor SRP55; Pre-mRNA-splicing factor SRP75; Protein X16; RGD1561347; serine and arginine rich splicing factor 3; serine and arginine rich splicing factor 4; serine and arginine rich splicing factor 5; serine and arginine rich splicing factor 6; serine/arginine-rich splicing factor 3; Serine/arginine-rich splicing factor 4; serine/arginine-rich splicing factor 5; Serine/arginine-rich splicing factor 6; SFRS3; Sfrs4; Sfrs5; Sfrs6; splicing factor; splicing factor arginine/serine-rich 5 (SRp40 HRS); splicing factor SRp55; splicing factor SRp55 homolog; splicing factor SRp55-1; splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich 3 (SRp20); splicing factor, arginine/serine-rich 4; splicing factor, arginine/serine-rich 4 (SRp75); splicing factor, arginine/serine-rich 5; splicing factor, arginine/serine-rich 5 (SRp40, HRS); splicing factor, arginine/serine-rich 6; splicing factor, arginine/serine-rich, 20-kD; splicing factor, arginine/serine-rich, 55 kDa; SR splicing factor 4; SR splicing factor 5; SR splicing factor 6; SRP001LB; Srp20; SRP40; SRP55; SRP55-1; SRP75; SRSF3; SRSF4; SRSF5; Srsf6; X16

more info or order :

Invitrogen product webpage