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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
ZO-1 Monoclonal Antibody (ZO1-1A12), FITC
catalog :
33-9111
quantity :
100 µg
price :
US 439
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
ZO1-1A12
reactivity :
human, mouse, rat, dogs, zebrafish
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 35
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:100; fig 4
Campos Y, Qiu X, Gomero E, Wakefield R, Horner L, Brutkowski W, et al. Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier. Nat Commun. 2016;7:11876 pubmed publisher
  • immunohistochemistry; mouse; fig 6
Pak J, Lee E, CRAFT C. The retinal phenotype of Grk1-/- is compromised by a Crb1 rd8 mutation. Mol Vis. 2015;21:1281-94 pubmed
  • immunohistochemistry - frozen section; rat; 1:100
Tang E, Mok K, Lee W, Cheng C. EB1 regulates tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier in male rats: an in vitro study. Endocrinology. 2015;156:680-93 pubmed publisher
  • immunocytochemistry; human; 1:100
Ferrer M, Corneo B, Davis J, Wan Q, Miyagishima K, King R, et al. A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium. Stem Cells Transl Med. 2014;3:911-22 pubmed publisher
  • immunohistochemistry - frozen section; zebrafish
Araya C, Tawk M, Girdler G, Costa M, Carmona Fontaine C, Clarke J. Mesoderm is required for coordinated cell movements within zebrafish neural plate in vivo. Neural Dev. 2014;9:9 pubmed publisher
  • immunohistochemistry; rat; 1:100
Qian X, Mruk D, Cheng C. Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*. PLoS ONE. 2013;8:e60656 pubmed publisher
  • immunocytochemistry; human
Sugimoto J, Sugimoto M, BERNSTEIN H, Jinno Y, Schust D. A novel human endogenous retroviral protein inhibits cell-cell fusion. Sci Rep. 2013;3:1462 pubmed publisher
  • immunocytochemistry; rat; 1:100
Lydka M, Bilinska B, Cheng C, Mruk D. Tumor necrosis factor ?-mediated restructuring of the Sertoli cell barrier in vitro involves matrix metalloprotease 9 (MMP9), membrane-bound intercellular adhesion molecule-1 (ICAM-1) and the actin cytoskeleton. Spermatogenesis. 2012;2:294-303 pubmed
  • immunohistochemistry - paraffin section; mouse; 1:50
Keay S, Leitzell S, Ochrzcin A, Clements G, Zhan M, Johnson D. A mouse model for interstitial cystitis/painful bladder syndrome based on APF inhibition of bladder epithelial repair: a pilot study. BMC Urol. 2012;12:17 pubmed
  • immunocytochemistry; human; fig 6
Edelstein A, Fink D, Musch M, Valuckaite V, Zaborina O, Grubjesic S, et al. Protective effects of nonionic triblock copolymers on bile acid-mediated epithelial barrier disruption. Shock. 2011;36:451-7 pubmed publisher
  • immunocytochemistry; human; fig 1
Wassmer S, Moxon C, Taylor T, Grau G, Molyneux M, Craig A. Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF. Cell Microbiol. 2011;13:198-209 pubmed publisher
  • immunocytochemistry; rat; 1:50; tbl 1
Su L, Cheng C, Mruk D. Adjudin-mediated Sertoli-germ cell junction disassembly affects Sertoli cell barrier function in vitro and in vivo. Int J Biochem Cell Biol. 2010;42:1864-75 pubmed publisher
  • flow cytometry; human; tbl 2
Semenov O, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch A, et al. Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation. Am J Obstet Gynecol. 2010;202:193.e1-193.e13 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:5; fig 4
  • western blot; rat; 1:500; fig 7
Sobarzo C, Lustig L, Ponzio R, Suescun M, Denduchis B. Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats. Microsc Res Tech. 2009;72:868-77 pubmed publisher
  • immunohistochemistry; rat; fig 5
Morishige N, Ko J, Liu Y, Chikama T, Nishida T. Localization of semaphorin 3A in the rat cornea. Exp Eye Res. 2008;86:669-74 pubmed publisher
  • immunocytochemistry; human
Roberts C, Rivera B, Grzybowski D, Mahmoud A, Weber P. Effect of low fluence diode laser irradiation on the hydraulic conductivity of perfused trabecular meshwork endothelial cell monolayers. Curr Eye Res. 2007;32:625-38 pubmed
  • immunohistochemistry - frozen section; rat; 1:100; tbl 1
Xia W, Wong C, Lee N, Lee W, Cheng C. Disruption of Sertoli-germ cell adhesion function in the seminiferous epithelium of the rat testis can be limited to adherens junctions without affecting the blood-testis barrier integrity: an in vivo study using an androgen suppression model. J Cell Physiol. 2005;205:141-57 pubmed
  • immunohistochemistry; rat; fig 9
Siu M, Mruk D, Lee W, Cheng C. Adhering junction dynamics in the testis are regulated by an interplay of beta 1-integrin and focal adhesion complex-associated proteins. Endocrinology. 2003;144:2141-63 pubmed
Li Y, Wei J, Liu H, Wang K, Jin S, Su Z, et al. An oxygen-adaptive interaction between SNHG12 and occludin maintains blood-brain barrier integrity. Cell Rep. 2022;39:110656 pubmed publisher
Becker H, Jamin C, Bervoets L, Boleij A, Xu P, Pierik M, et al. Higher Prevalence of Bacteroides fragilis in Crohn's Disease Exacerbations and Strain-Dependent Increase of Epithelial Resistance. Front Microbiol. 2021;12:598232 pubmed publisher
Matsumoto H, Mukai R, Hoshino J, Oda M, Matsuzaki T, Ishizaki Y, et al. Choroidal congestion mouse model: Could it serve as a pachychoroid model?. PLoS ONE. 2021;16:e0246115 pubmed publisher
Ren Y, Dong X, Zhao H, Feng J, Chen B, Zhou Y, et al. Myeloid-derived suppressor cells improve corneal graft survival through suppressing angiogenesis and lymphangiogenesis. Am J Transplant. 2021;21:552-566 pubmed publisher
Lu Y, Deng R, You H, Xu Y, Antos C, Sun J, et al. Asymmetric Stratification-Induced Polarity Loss and Coordinated Individual Cell Movements Drive Directional Migration of Vertebrate Epithelium. Cell Rep. 2020;33:108246 pubmed publisher
Miller P, Chen C, Wang Y, Gao E, Shuler M. Multiorgan microfluidic platform with breathable lung chamber for inhalation or intravenous drug screening and development. Biotechnol Bioeng. 2020;117:486-497 pubmed publisher
Araya C, Häkkinen H, Carcamo L, Cerda M, Savy T, Rookyard C, et al. Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate. Sci Rep. 2019;9:1835 pubmed publisher
Angulo Urarte A, Casado P, Castillo S, Kobialka P, Kotini M, Figueiredo A, et al. Endothelial cell rearrangements during vascular patterning require PI3-kinase-mediated inhibition of actomyosin contractility. Nat Commun. 2018;9:4826 pubmed publisher
Miller P, Shuler M. Design and demonstration of a pumpless 14 compartment microphysiological system. Biotechnol Bioeng. 2016;113:2213-27 pubmed publisher
Wan H, Mruk D, Wong C, Cheng C. Perfluorooctanesulfonate (PFOS) perturbs male rat Sertoli cell blood-testis barrier function by affecting F-actin organization via p-FAK-Tyr(407): an in vitro study. Endocrinology. 2014;155:249-62 pubmed publisher
Qian X, Mruk D, Wong E, Lie P, Cheng C. Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes. Endocrinology. 2013;154:1907-20 pubmed publisher
Raiko L, Siljamäki E, Mahoney M, Putaala H, Suominen E, Peltonen J, et al. Hailey-Hailey disease and tight junctions: Claudins 1 and 4 are regulated by ATP2C1 gene encoding Ca(2+) /Mn(2+) ATPase SPCA1 in cultured keratinocytes. Exp Dermatol. 2012;21:586-91 pubmed publisher
Kuo T, Chen C, Baron D, Onder T, Loewer S, Almeida S, et al. Midbody accumulation through evasion of autophagy contributes to cellular reprogramming and tumorigenicity. Nat Cell Biol. 2011;13:1214-23 pubmed publisher
Lie P, Cheng C, Mruk D. Interleukin-1alpha is a regulator of the blood-testis barrier. FASEB J. 2011;25:1244-53 pubmed publisher
Sarkar O, Mathur P, Cheng C, Mruk D. Interleukin 1 alpha (IL1A) is a novel regulator of the blood-testis barrier in the rat. Biol Reprod. 2008;78:445-54 pubmed
Lie P, Xia W, Wang C, Mruk D, Yan H, Wong C, et al. Dynamin II interacts with the cadherin- and occludin-based protein complexes at the blood-testis barrier in adult rat testes. J Endocrinol. 2006;191:571-86 pubmed
Bandyopadhyay B, Swaim W, Liu X, Redman R, Patterson R, Ambudkar I. Apical localization of a functional TRPC3/TRPC6-Ca2+-signaling complex in polarized epithelial cells. Role in apical Ca2+ influx. J Biol Chem. 2005;280:12908-16 pubmed
product information
Product Type :
Antibody
Product Name :
ZO-1 Monoclonal Antibody (ZO1-1A12), FITC
Catalog # :
33-9111
Quantity :
100 µg
Pricing :
US 439
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Canine, Human
Applications :
ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 1-10 µg/mL, Western Blot: 1-2 µg/mL
Species :
Canine, Human
Clone :
ZO1-1A12
Isotype :
IgG1, kappa
Storage :
4° C, store in dark
Description :
ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
Immunogen :
Human recombinant ZO-1 fusion protein encompassing amino acids 334-634
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 1-10 µg/mL, Western Blot: 1-2 µg/mL
Aliases :
DKFZp686M05161; I79_005485; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); tight junction protein ZO-1; Tjp1; zo1; ZO-1; ZO-1 MDCK; zona occludens 1; zona occludens protein 1; zonula occludens 1; zonula occludens 1 protein; zonula occludens protein 1
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA