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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Ankyrin G Monoclonal Antibody (4G3F8)
catalog :
33-8800
quantity :
100 ug
price :
US 451.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4G3F8
reactivity :
human, mouse, rat, dogs, pigs , domestic sheep, domestic rabbit
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 35
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:300; loading ...; fig 3c, e3d
  • immunohistochemistry; mouse; 1:300; loading ...; fig 5i
Kiryu Seo S, Matsushita R, Tashiro Y, Yoshimura T, Iguchi Y, Katsuno M, et al. Impaired disassembly of the axon initial segment restricts mitochondrial entry into damaged axons. EMBO J. 2022;41:e110486 pubmed publisher
  • immunocytochemistry; rat; 1:500; loading ...; fig 2b
König H, Schwamborn R, Andresen S, Kinsella S, Watters O, Fenner B, et al. NF-κB regulates neuronal ankyrin-G via a negative feedback loop. Sci Rep. 2017;7:42006 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 6
Vilmont V, Cadot B, Ouanounou G, Gomes E. A system for studying mechanisms of neuromuscular junction development and maintenance. Development. 2016;143:2464-77 pubmed publisher
  • immunocytochemistry; rat; 1:300; fig 3
Zhu G, Du L, Jin L, Offenhäusser A. Effects of Morphology Constraint on Electrophysiological Properties of Cortical Neurons. Sci Rep. 2016;6:23086 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; fig 4
Gao Y, Heldt S. Enrichment of GABAA Receptor α-Subunits on the Axonal Initial Segment Shows Regional Differences. Front Cell Neurosci. 2016;10:39 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; fig 2
Schütze S, Orozco I, Jentsch T. KCNQ Potassium Channels Modulate Sensitivity of Skin Down-hair (D-hair) Mechanoreceptors. J Biol Chem. 2016;291:5566-75 pubmed publisher
  • immunohistochemistry; pigs ; 1:100
  • western blot; pigs ; 1:200
Park D, Cerrone M, Morley G, Vasquez C, Fowler S, Liu N, et al. Genetically engineered SCN5A mutant pig hearts exhibit conduction defects and arrhythmias. J Clin Invest. 2015;125:403-12 pubmed publisher
  • immunohistochemistry; human; 1:100
Higurashi N, Uchida T, Lossin C, Misumi Y, Okada Y, Akamatsu W, et al. A human Dravet syndrome model from patient induced pluripotent stem cells. Mol Brain. 2013;6:19 pubmed publisher
  • immunohistochemistry - frozen section; domestic sheep
Chkourko H, Guerrero Serna G, Lin X, Darwish N, Pohlmann J, Cook K, et al. Remodeling of mechanical junctions and of microtubule-associated proteins accompany cardiac connexin43 lateralization. Heart Rhythm. 2012;9:1133-1140.e6 pubmed publisher
  • immunohistochemistry; rat
Kang H, Tian L, Son Y, Zuo Y, Procaccino D, Love F, et al. Regulation of the intermediate filament protein nestin at rodent neuromuscular junctions by innervation and activity. J Neurosci. 2007;27:5948-57 pubmed
  • immunohistochemistry; human; 10 ug/ml; fig 6
Allard B, Magloire H, Couble M, Maurin J, Bleicher F. Voltage-gated sodium channels confer excitability to human odontoblasts: possible role in tooth pain transmission. J Biol Chem. 2006;281:29002-10 pubmed
  • immunohistochemistry - paraffin section; mouse
Tang Y, Katuri V, Iqbal S, Narayan T, Wang Z, Lu R, et al. ELF a beta-spectrin is a neuronal precursor cell marker in developing mammalian brain; structure and organization of the elf/beta-G spectrin gene. Oncogene. 2002;21:5255-67 pubmed
Kwan H, Chan Z, Bi X, Kutkowska J, Pr xf3 szy x144 ski T, Chan C, et al. Nerve-independent formation of membrane infoldings at topologically complex postsynaptic apparatus by caveolin-3. Sci Adv. 2023;9:eadg0183 pubmed publisher
Teunissen M, Lewerissa E, van Hugte E, Wang S, Ockeloen C, Koolen D, et al. ANK2 loss-of-function variants are associated with epilepsy, and lead to impaired axon initial segment plasticity and hyperactive network activity in hiPSC-derived neuronal networks. Hum Mol Genet. 2023;32:2373-2385 pubmed publisher
Le Gratiet K, Anderson C, Puente N, Grandes P, Copas C, Nahirney P, et al. Differential Subcellular Distribution and Release Dynamics of Cotransmitted Cholinergic and GABAergic Synaptic Inputs Modify Dopaminergic Neuronal Excitability. J Neurosci. 2022;42:8670-8693 pubmed publisher
Chen Z, Peng L, Zhao M, Li Y, Takahiko M, Tao L, et al. Differences in action potential propagation speed and axon initial segment plasticity between neurons from Sprague-Dawley rats and C57BL/6 mice. Zool Res. 2022;43:615-633 pubmed publisher
McGonigal R, Campbell C, Barrie J, Yao D, Cunningham M, Crawford C, et al. Schwann cell nodal membrane disruption triggers bystander axonal degeneration in a Guillain-Barré syndrome mouse model. J Clin Invest. 2022;132: pubmed publisher
Cunningham M, McGonigal R, Barrie J, Yao D, Willison H. Real time imaging of intra-axonal calcium flux in an explant mouse model of axonal Guillain-Barré syndrome. Exp Neurol. 2022;355:114127 pubmed publisher
Wang Y, Guan M, Zhang Y, Zhanghao K, Xi P. Glucose increases the length and spacing of the lattice structure of the axon initial segment. Microsc Res Tech. 2022;85:2679-2691 pubmed publisher
Risner M, Pasini S, Chamling X, McGrady N, Goldberg J, Zack D, et al. Intrinsic Morphologic and Physiologic Development of Human Derived Retinal Ganglion Cells In Vitro. Transl Vis Sci Technol. 2021;10:1 pubmed publisher
Behrendt L, Hoischen C, Kaether C. Disease-causing mutated ATLASTIN 3 is excluded from distal axons and reduces axonal autophagy. Neurobiol Dis. 2021;155:105400 pubmed publisher
Lindhout F, Portegies S, Kooistra R, Herstel L, Stucchi R, Hummel J, et al. Centrosome-mediated microtubule remodeling during axon formation in human iPSC-derived neurons. EMBO J. 2021;40:e106798 pubmed publisher
Lindhout F, Kooistra R, Portegies S, Herstel L, Stucchi R, Snoek B, et al. Quantitative mapping of transcriptome and proteome dynamics during polarization of human iPSC-derived neurons. elife. 2020;9: pubmed publisher
Caccavano A, Bozzelli P, Forcelli P, Pak D, Wu J, Conant K, et al. Inhibitory Parvalbumin Basket Cell Activity is Selectively Reduced during Hippocampal Sharp Wave Ripples in a Mouse Model of Familial Alzheimer's Disease. J Neurosci. 2020;40:5116-5136 pubmed publisher
Yang R, Walder Christensen K, Lalani S, Yan H, García Prieto I, Alvarez S, et al. Neurodevelopmental mutation of giant ankyrin-G disrupts a core mechanism for axon initial segment assembly. Proc Natl Acad Sci U S A. 2019;116:19717-19726 pubmed publisher
Domke L, Franke W. The cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions-a novel architectonic cell-cell junction system. Cell Tissue Res. 2019;375:451-482 pubmed publisher
Vukoja A, Rey U, Petzoldt A, Ott C, Vollweiter D, Quentin C, et al. Presynaptic Biogenesis Requires Axonal Transport of Lysosome-Related Vesicles. Neuron. 2018;99:1216-1232.e7 pubmed publisher
Homma N, Zhou R, Naseer M, Chaudhary A, Al Qahtani M, Hirokawa N. KIF2A regulates the development of dentate granule cells and postnatal hippocampal wiring. elife. 2018;7: pubmed publisher
O Hare Doig R, Chiha W, Giacci M, Yates N, Bartlett C, Smith N, et al. Specific ion channels contribute to key elements of pathology during secondary degeneration following neurotrauma. BMC Neurosci. 2017;18:62 pubmed publisher
Gumy L, Katrukha E, Grigoriev I, Jaarsma D, Kapitein L, Akhmanova A, et al. MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons. Neuron. 2017;94:347-362.e7 pubmed publisher
Colakoglu G, Bergstrom Tyrberg U, Berglund E, Ranscht B. Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system. Proc Natl Acad Sci U S A. 2014;111:E394-403 pubmed publisher
Lopez C, Metral S, Eladari D, Drevensek S, Gane P, Chambrey R, et al. The ammonium transporter RhBG: requirement of a tyrosine-based signal and ankyrin-G for basolateral targeting and membrane anchorage in polarized kidney epithelial cells. J Biol Chem. 2005;280:8221-8 pubmed
Edgar J, McLaughlin M, Yool D, Zhang S, Fowler J, Montague P, et al. Oligodendroglial modulation of fast axonal transport in a mouse model of hereditary spastic paraplegia. J Cell Biol. 2004;166:121-31 pubmed
Malhotra J, Koopmann M, Kazen Gillespie K, Fettman N, Hortsch M, Isom L. Structural requirements for interaction of sodium channel beta 1 subunits with ankyrin. J Biol Chem. 2002;277:26681-8 pubmed
Mathis C, Denisenko Nehrbass N, Girault J, Borrelli E. Essential role of oligodendrocytes in the formation and maintenance of central nervous system nodal regions. Development. 2001;128:4881-90 pubmed
product information
Product Type :
Antibody
Product Name :
Ankyrin G Monoclonal Antibody (4G3F8)
Catalog # :
33-8800
Quantity :
100 ug
Price :
US 451.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Canine, Human, Mouse, Rabbit, Rat
Applications :
ELISA: Assay-dependent, Immunocytochemistry: 2-5 ug/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000
Species :
Canine, Human, Mouse, Rabbit, Rat
Clone :
4G3F8
Isotype :
IgG1, kappa
Storage :
-20 C
Description :
Ankyrin-G is found in several tissues including brain where, due to tissue-specific RNA processing, it is expressed as two different isoforms, localized to axon initial segments and nodes of Ranvier. Ankyrin-G is also found at sites of epithelial cell-cell contact and in the intracellular membranes of some cells. Ankyrin-G isoforms contain a unique stretch of sequence highly enriched in serine and threonine residues immediately following a globular head domain. Ankyrin-G is thought to participate in the maintenance and targeting of ion channels and cell adhesion molecules to the nodes of Ranvier and to axon initial segments.
Immunogen :
A synthetic peptide derived from the spectrin-binding domain of the human ankyrin G protein.
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Immunocytochemistry: 2-5 ug/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000
Aliases :
2900054D09Rik; AI314020; ANK3; ANK-3; AnkG; ankyrin 3; ankyrin 3 (G); ankyrin 3, epithelial; ankyrin 3, node of Ranvier (ankyrin G); ankyrin G; ankyrin-3; Ankyrin-G; brain-specific ankyrin-G; LOW QUALITY PROTEIN: ankyrin-3; MRT37
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA