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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Bax Monoclonal Antibody (2D2)
catalog :
33-6400
quantity :
100 µg
price :
US 449.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2D2
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 22
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 5a
Martínez Castillo M, Bonilla Moreno R, Alemán Lazarini L, Meraz Rios M, Orozco L, Cedillo Barron L, et al. A Subpopulation of the K562 Cells Are Killed by Curcumin Treatment after G2/M Arrest and Mitotic Catastrophe. PLoS ONE. 2016;11:e0165971 pubmed publisher
  • immunocytochemistry; human; fig 3
Raemy E, Montessuit S, Pierredon S, van Kampen A, Vaz F, Martinou J. Cardiolipin or MTCH2 can serve as tBID receptors during apoptosis. Cell Death Differ. 2016;23:1165-74 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:25
Gurzu S, Kádár Z, Sugimura H, Bara T, Hălmaciu I, Jung I. Gastric cancer in young vs old Romanian patients: immunoprofile with emphasis on maspin and mena protein reactivity. APMIS. 2015;123:223-33 pubmed publisher
  • western blot; human; fig 5
Piedfer M, Bouchet S, Tang R, Billard C, Dauzonne D, Bauvois B. p70S6 kinase is a target of the novel proteasome inhibitor 3,3'-diamino-4'-methoxyflavone during apoptosis in human myeloid tumor cells. Biochim Biophys Acta. 2013;1833:1316-28 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
Jung Y, Joo K, Seong D, Choi Y, Kong D, Kim Y, et al. Identification of prognostic biomarkers for glioblastomas using protein expression profiling. Int J Oncol. 2012;40:1122-32 pubmed publisher
  • western blot; human; fig 8
Piedfer M, Dauzonne D, Tang R, N Guyen J, Billard C, Bauvois B. Aminopeptidase-N/CD13 is a potential proapoptotic target in human myeloid tumor cells. FASEB J. 2011;25:2831-42 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50
Sun B, Sun Y, Wang J, Zhao X, Wang X, Hao X. Extent, relationship and prognostic significance of apoptosis and cell proliferation in synovial sarcoma. Eur J Cancer Prev. 2006;15:258-65 pubmed
  • immunohistochemistry - paraffin section; human; 1:50
Cherian S, Bobryshev Y, Tran D, Sivaraman A, Lord R, Cherian K. Cellular destruction following transmyocardial laser revascularization (TMR). J Mol Histol. 2005;36:275-80 pubmed
  • immunohistochemistry; human; 1:100; tbl 2
Bassarova A, Nesland J, Sedloev T, Danielsen H, Christova S. Pilomatrix carcinoma with lymph node metastases. J Cutan Pathol. 2004;31:330-5 pubmed
  • immunohistochemistry - paraffin section; human; 1:50; tbl 3
Kim L, Nadarajah V, Peh S, Poppema S. Expression of Bcl-2 family members and presence of Epstein-Barr virus in the regulation of cell growth and death in classical Hodgkin's lymphoma. Histopathology. 2004;44:257-67 pubmed
  • immunohistochemistry; human; 1:40; fig 2
Rassidakis G, Jones D, Lai R, Ramalingam P, Sarris A, McDonnell T, et al. BCL-2 family proteins in peripheral T-cell lymphomas: correlation with tumour apoptosis and proliferation. J Pathol. 2003;200:240-8 pubmed
  • immunohistochemistry; human; 1:150; fig 1-4
Korkolopoulou P, Lazaris A, Konstantinidou A, Kavantzas N, Patsouris E, Christodoulou P, et al. Differential expression of bcl-2 family proteins in bladder carcinomas. Relationship with apoptotic rate and survival. Eur Urol. 2002;41:274-83 pubmed
  • immunohistochemistry - paraffin section; human; 1:100; tbl 2
Sjöström J, Blomqvist C, von Boguslawski K, Bengtsson N, Mjaaland I, Malmström P, et al. The predictive value of bcl-2, bax, bcl-xL, bag-1, fas, and fasL for chemotherapy response in advanced breast cancer. Clin Cancer Res. 2002;8:811-6 pubmed
  • western blot; human; fig 4
Goetzl E, Kong Y, Mei B. Lysophosphatidic acid and sphingosine 1-phosphate protection of T cells from apoptosis in association with suppression of Bax. J Immunol. 1999;162:2049-56 pubmed
Wang J, Luo J, Wu X, Gao Z. Circular RNA_0000629 Suppresses Bladder Cancer Progression Mediating MicroRNA-1290/CDC73. Cancer Manag Res. 2021;13:2701-2715 pubmed publisher
Plackal Adimuriyil George B, Kumar N, Abrahamse H, Ray S. Apoptotic efficacy of multifaceted biosynthesized silver nanoparticles on human adenocarcinoma cells. Sci Rep. 2018;8:14368 pubmed publisher
Gao D, Zhang J, Bai L, Li F, Dong Y, Li Q. Melittin induces NSCLC apoptosis via inhibition of miR-183. Onco Targets Ther. 2018;11:4511-4523 pubmed publisher
Pollack A, Kwon D, Walker G, Khor L, Horwitz E, Buyyounouski M, et al. Prospective Validation of Diagnostic Tumor Biomarkers in Men Treated With Radiotherapy for Prostate Cancer. J Natl Cancer Inst. 2017;109:1-8 pubmed publisher
Pagliaro L, Keyhani A, Williams D, Woods D, Liu B, Perrotte P, et al. Repeated intravesical instillations of an adenoviral vector in patients with locally advanced bladder cancer: a phase I study of p53 gene therapy. J Clin Oncol. 2003;21:2247-53 pubmed
Gascoyne D, Kypta R, Vivanco M. Glucocorticoids inhibit apoptosis during fibrosarcoma development by transcriptionally activating Bcl-xL. J Biol Chem. 2003;278:18022-9 pubmed
Stadheim T, Suh N, Ganju N, Sporn M, Eastman A. The novel triterpenoid 2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid (CDDO) potently enhances apoptosis induced by tumor necrosis factor in human leukemia cells. J Biol Chem. 2002;277:16448-55 pubmed
Rassidakis G, Medeiros L, McDonnell T, Viviani S, Bonfante V, Nadali G, et al. BAX expression in Hodgkin and Reed-Sternberg cells of Hodgkin's disease: correlation with clinical outcome. Clin Cancer Res. 2002;8:488-93 pubmed
product information
Product Type :
Antibody
Product Name :
Bax Monoclonal Antibody (2D2)
Catalog # :
33-6400
Quantity :
100 µg
Price :
US 449.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Mouse, Non-human primate
Applications :
ELISA: Assay-dependent, Immunohistochemistry (Paraffin): 1:50-1:500, Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:1,000
Species :
Human, Mouse, Non-human primate
Clone :
2D2
Isotype :
IgG1
Storage :
-20°C
Description :
BAX is a members of the Bcl-2 Family and plays an important role in regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, BAX is considered to have a pro-apoptotic function. Regulation of apoptosis is supposed to involve both homo- and heterodimerization of different isoforms of BAX and Bcl-2. The Bax gene encodes different isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa), whereas both isoforms contain the BH1, BH2 and BH3 domains, Bax beta has a unique carboxyl terminus and does not contain a hydrophobic transmembrane domain. Bcl-2 is also expressed in different Isoforms. Bcl-2 beta differs in the 3' UTR and coding region compared to variant alpha. Bcl-2 beta is shorter (22 kDa) and has a distinct C-terminus compared to Bcl-2 alpha (26 kDa). BAX is a member of the BCL-2 family of proteins, which function as regulators of apoptosis. Overexpression of BAX functions to promote cell death. BAX can form homodimers and is also able to heterodimerize with other BCL-2 related proteins.
Immunogen :
Synthetic peptide corresponding to a sequence at the N-terminal of the human protein.
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Immunohistochemistry (Paraffin): 1:50-1:500, Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:1,000
Aliases :
apoptosis regulator BAX; Apoptosis regulator Bcl-X; Bax; Bax zeta; BAX-ALPHA; Bax-alpha protein; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; BCL2 associated X protein; BCL2 associated X, apoptosis regulator; BCL2 like 1; BCL2-associated protein; Bcl-2-associated protein Bax; Bcl2-associated protein Bax; Bcl-2-associated X protein; BCL2-associated X protein; BCL2-associated X protein omega; BCL2L; BCL2L1; Bcl2-L-1; BCL2L4; Bcl2-L-4; BCL2-like 1; bcl-2-like protein 1; Bcl-2-like protein 4; BCLX; Bcl-X; BCL-XL/S; PPP1R52; protein phosphatase 1, regulatory subunit 52
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA