Bcl-2 Monoclonal Antibody (10C4) | Invitrogen 33-6100 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Bcl-2 Monoclonal Antibody (10C4)

catalog :

33-6100

quantity :

100 µg

price :

US 406.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10C4

reactivity :

mouse, rat

application :

western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 6d	Visekruna A, Hartmann S, Sillke Y, Glauben R, Fischer F, Raifer H, et al. Intestinal development and homeostasis require activation and apoptosis of diet-reactive T cells. J Clin Invest. 2019;129:1972-1983 pubmed publisher
flow cytometry; mouse; 1:200; loading ...; fig 5e	Zhu L, Xie X, Zhang L, Wang H, Jie Z, Zhou X, et al. TBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival. Nat Commun. 2018;9:2812 pubmed publisher
western blot; rat; fig 3b	Jiang P, Zhang D, Qiu H, Yi X, Zhang Y, Cao Y, et al. Tiron ameliorates high glucose-induced cardiac myocyte apoptosis by PKCÎ´-dependent inhibition of osteopontin. Clin Exp Pharmacol Physiol. 2017;44:760-770 pubmed publisher
western blot; mouse	Götschel F, Kern C, Lang S, Sparna T, Markmann C, Schwager J, et al. Inhibition of GSK3 differentially modulates NF-kappaB, CREB, AP-1 and beta-catenin signaling in hepatocytes, but fails to promote TNF-alpha-induced apoptosis. Exp Cell Res. 2008;314:1351-66 pubmed publisher
immunohistochemistry - frozen section; rat; 2.5 ug/ml; fig 7	Yamanaka T, Helgeland L, Farstad I, Fukushima H, Midtvedt T, Brandtzaeg P. Microbial colonization drives lymphocyte accumulation and differentiation in the follicle-associated epithelium of Peyer's patches. J Immunol. 2003;170:816-22 pubmed
immunocytochemistry; rat; 1:100; fig 1 western blot; rat; 1:1000; fig 1	Kaufmann T, Schlipf S, Sanz J, Neubert K, Stein R, Borner C. Characterization of the signal that directs Bcl-x(L), but not Bcl-2, to the mitochondrial outer membrane. J Cell Biol. 2003;160:53-64 pubmed
	Zhai G, Liang W, Xu Y. High Expression of Lysophosphatidic Acid Induces Nerve Injury in LSS Patients via AKT Mediated NF-κB p65 Pathway. Front Pharmacol. 2021;12:641435 pubmed publisher
	Taira N, Yamaguchi T, Kimura J, Lu Z, Fukuda S, Higashiyama S, et al. Induction of amphiregulin by p53 promotes apoptosis via control of microRNA biogenesis in response to DNA damage. Proc Natl Acad Sci U S A. 2014;111:717-22 pubmed publisher
	Tabor Godwin J, Tsueng G, Sayen M, Gottlieb R, Feuer R. The role of autophagy during coxsackievirus infection of neural progenitor and stem cells. Autophagy. 2012;8:938-53 pubmed publisher
	Zhu Y, Swanson B, Wang M, Hildeman D, Schaefer B, Liu X, et al. Constitutive association of the proapoptotic protein Bim with Bcl-2-related proteins on mitochondria in T cells. Proc Natl Acad Sci U S A. 2004;101:7681-6 pubmed

product information

Product Type :

Antibody

Product Name :

Bcl-2 Monoclonal Antibody (10C4)

Catalog # :

33-6100

Quantity :

100 µg

Price :

US 406.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Mouse, Rat

Applications :

ELISA: Assay-dependent, Western Blot: Assay-dependent

Species :

Mouse, Rat

Clone :

10C4

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

Immunogen :

Synthetic peptide corresponding to a sequence at the amino terminal of the murine Bcl-2 protein.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Western Blot: Assay-dependent

Aliases :

apoptosis regulator Bcl-2; AW986256; B cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; BCL2; bcl-2; BCL2 apoptosis regulator; Bcl-2 protein; BCL2, apoptosis regulator; Bcl2-like protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; OTTHUMP00000163680; PPP1R50; protein phosphatase 1, regulatory subunit 50

more info or order :

Invitrogen product webpage