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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
SPARC Monoclonal Antibody (ON1-1)
catalog :
33-5500
quantity :
100 ug
price :
US 484.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ON1-1
reactivity :
human, rat, dogs, bovine, pigs , domestic rabbit
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 30
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; dogs; fig 6
Ozdal Kurt F, Tuglu I, Vatansever H, Tong S, Sen B, Deliloglu Gurhan S. The effect of different implant biomaterials on the behavior of canine bone marrow stromal cells during their differentiation into osteoblasts. Biotech Histochem. 2016;91:412-22 pubmed publisher
  • immunocytochemistry; human; fig 1
Ozdal Kurt F, Sen B, Tuglu I, Vatansever S, Türk B, Deliloglu Gurhan I. Attachment and growth of dental pulp stem cells on dentin in presence of extra calcium. Arch Oral Biol. 2016;68:131-41 pubmed publisher
  • ELISA; human
Jokerst J, Chen Z, Xu L, Nolley R, Chang E, Mitchell B, et al. A Magnetic Bead-Based Sensor for the Quantification of Multiple Prostate Cancer Biomarkers. PLoS ONE. 2015;10:e0139484 pubmed publisher
  • immunohistochemistry; human; 1:400; fig 1
Bertino E, Williams T, Nana Sinkam S, Shilo K, Chatterjee M, Mo X, et al. Stromal Caveolin-1 Is Associated With Response and Survival in a Phase II Trial of nab-Paclitaxel With Carboplatin for Advanced NSCLC Patients. Clin Lung Cancer. 2015;16:466-74 pubmed publisher
  • immunohistochemistry; dogs; fig 5
Özdal Kurt F, Tuğlu I, Vatansever H, Tong S, Deliloğlu Gürhan S. The effect of autologous bone marrow stromal cells differentiated on scaffolds for canine tibial bone reconstruction. Biotech Histochem. 2015;90:516-28 pubmed publisher
  • immunohistochemistry; human; 1:100
Ren K, Yao N, Wang G, Tian L, Ma J, Shi X, et al. Vasculogenic mimicry: a new prognostic sign of human osteosarcoma. Hum Pathol. 2014;45:2120-9 pubmed publisher
  • ELISA; human; fig 1
Xu L, Ping F, Yin J, Xiao X, Xiang H, Ballantyne C, et al. Elevated plasma SPARC levels are associated with insulin resistance, dyslipidemia, and inflammation in gestational diabetes mellitus. PLoS ONE. 2013;8:e81615 pubmed publisher
  • immunohistochemistry; human; 1:1000; fig 3
Chen J, Espinosa I, Lin A, Liao O, van de Rijn M, West R. Stromal responses among common carcinomas correlated with clinicopathologic features. Clin Cancer Res. 2013;19:5127-35 pubmed publisher
  • immunohistochemistry - paraffin section; human; 10 ug/ml; fig 2
Triulzi T, Casalini P, Sandri M, Ratti M, Carcangiu M, Colombo M, et al. Neoplastic and stromal cells contribute to an extracellular matrix gene expression profile defining a breast cancer subtype likely to progress. PLoS ONE. 2013;8:e56761 pubmed publisher
  • immunohistochemistry; rat; fig 5
Ozturk F, Babacan H, Inan S, Gumus C. Effects of bisphosphonates on sutural bone formation and relapse: A histologic and immunohistochemical study. Am J Orthod Dentofacial Orthop. 2011;140:e31-41 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:6000; fig 1
Daugaard S, Christensen L, Høgdall E. Markers aiding the diagnosis of chondroid tumors: an immunohistochemical study including osteonectin, bcl-2, cox-2, actin, calponin, D2-40 (podoplanin), mdm-2, CD117 (c-kit), and YKL-40. APMIS. 2009;117:518-25 pubmed publisher
  • immunohistochemistry; human
Andersen T, Sondergaard T, Skorzynska K, Dagnaes Hansen F, Plesner T, Hauge E, et al. A physical mechanism for coupling bone resorption and formation in adult human bone. Am J Pathol. 2009;174:239-47 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:500
Hong S, Kelly D, Griffith M, Omura N, Li A, Li C, et al. Multiple genes are hypermethylated in intraductal papillary mucinous neoplasms of the pancreas. Mod Pathol. 2008;21:1499-507 pubmed publisher
  • immunocytochemistry; human; 5 ug/ml; fig 3
  • western blot; human; fig 2
Pan M, Chang H, Chuang L, Hung W. The nonsteroidal anti-inflammatory drug NS398 reactivates SPARC expression via promoter demethylation to attenuate invasiveness of lung cancer cells. Exp Biol Med (Maywood). 2008;233:456-62 pubmed publisher
  • immunohistochemistry - paraffin section; human
Infante J, Matsubayashi H, Sato N, Tonascia J, Klein A, Riall T, et al. Peritumoral fibroblast SPARC expression and patient outcome with resectable pancreatic adenocarcinoma. J Clin Oncol. 2007;25:319-25 pubmed
  • immunohistochemistry; human; 1:200; fig 8B
  • western blot; human; 1:1000
Chlenski A, Liu S, Guerrero L, Yang Q, Tian Y, Salwen H, et al. SPARC expression is associated with impaired tumor growth, inhibited angiogenesis and changes in the extracellular matrix. Int J Cancer. 2006;118:310-6 pubmed
  • immunohistochemistry; human; fig 2
Sato N, Fukushima N, Maehara N, Matsubayashi H, Koopmann J, Su G, et al. SPARC/osteonectin is a frequent target for aberrant methylation in pancreatic adenocarcinoma and a mediator of tumor-stromal interactions. Oncogene. 2003;22:5021-30 pubmed
Şentürk A, Sahin A, Armutlu A, Kiremit M, Acar O, Erdem S, et al. Quantitative Proteomics Identifies Secreted Diagnostic Biomarkers as well as Tumor-Dependent Prognostic Targets for Clear Cell Renal Cell Carcinoma. Mol Cancer Res. 2021;19:1322-1337 pubmed publisher
Žlajpah M, Bostjancic E, Tepes B, Zidar N. Expression of Extracellular Matrix-Related Genes and Their Regulatory microRNAs in Problematic Colorectal Polyps. Cancers (Basel). 2020;12: pubmed publisher
Laçin N, İzol S, Ipek F, Tuncer M. Ganoderma lucidum, a promising agent possessing antioxidant and anti-inflammatory effects for treating calvarial defects with graft application in rats. Acta Cir Bras. 2019;34:e201900904 pubmed publisher
Laçin N, Deveci E. Short-term use of resveratrol in alloplastic graft material applied with calvarial bone defects in rats. Acta Cir Bras. 2019;34:e201900704 pubmed publisher
La xe7 in N, x130 zol B, G xf6 kalp xd6 zkorkmaz E, Deveci B, Deveci E. Effects of alloplastic graft material combined with a topical ozone application on calvarial bone defects in rats. Folia Morphol (Warsz). 2020;79:528-547 pubmed publisher
Laçin N, İzol B, Ozkorkmaz E, Deveci B, Tuncer M. The effect of graft application and simvastatin treatment on tibial bone defect in rats. A histological and immunohistochemical study. Acta Cir Bras. 2019;34:e201900408 pubmed publisher
López Moncada F, Torres M, Castellon E, Contreras H. Secreted protein acidic and rich in cysteine (SPARC) induces epithelial-mesenchymal transition, enhancing migration and invasion, and is associated with high Gleason score in prostate cancer. Asian J Androl. 2019;21:557-564 pubmed publisher
Baloğlu M, Gokalp Ozkorkmaz E. Biochemical and immunohistochemical investigations on bone formation and remodelling in ovariectomized rats with tamoxifen citrate administration. Folia Morphol (Warsz). 2019;: pubmed publisher
Laçin N, İzol B, Ozkorkmaz E, Deveci B, Tuncer M. The effect of graft application and allopurinol treatment on calvarial bone defect in rats1. Acta Cir Bras. 2019;34:e201900306 pubmed publisher
Laçin N, İzol B, Tuncer M, Gokalp Ozkorkmaz E, Deveci B, Deveci E. Effects of formaldehyde on VEGF, MMP2, and Osteonectin levels in periodontal membrane and alveolar bone in the rats. Folia Morphol (Warsz). 2018;: pubmed publisher
Andriani F, Landoni E, Mensah M, Facchinetti F, Miceli R, Tagliabue E, et al. Diagnostic role of circulating extracellular matrix-related proteins in non-small cell lung cancer. BMC Cancer. 2018;18:899 pubmed publisher
Higgins J, Wang L, Kambham N, Montgomery K, Mason V, Vogelmann S, et al. Gene expression in the normal adult human kidney assessed by complementary DNA microarray. Mol Biol Cell. 2004;15:649-56 pubmed
Fromigue O, Louis K, Dayem M, Milanini J, Pages G, Tartare Deckert S, et al. Gene expression profiling of normal human pulmonary fibroblasts following coculture with non-small-cell lung cancer cells reveals alterations related to matrix degradation, angiogenesis, cell growth and survival. Oncogene. 2003;22:8487-97 pubmed
product information
Product Type :
Antibody
Product Name :
SPARC Monoclonal Antibody (ON1-1)
Catalog # :
33-5500
Quantity :
100 ug
Price :
US 484.00
Clonality :
Monoclonal
Purity :
Ion-exchange chromatography
Host :
Mouse
Reactivity :
Bovine, Human, Porcine, Rabbit
Applications :
ELISA: Assay-dependent, Flow Cytometry: 1 ug/mL, Immunohistochemistry: 5-10 ug/mL, Western Blot: 5-10 ug/mL
Species :
Bovine, Human, Porcine, Rabbit
Clone :
ON1-1
Isotype :
IgG1
Storage :
4 C
Description :
SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
Immunogen :
Full-length Bovine bone osteonectin (SPARC)
Format :
Lyophilized
Applications w/Dilutions :
ELISA: Assay-dependent, Flow Cytometry: 1 ug/mL, Immunohistochemistry: 5-10 ug/mL, Western Blot: 5-10 ug/mL
Aliases :
Basement membrane protein 40; basement-membrane protein 40; BM40; BM-40; Cysteine rich protein; OI17; ON; Osteonectin; osteonectin precursor; Secreted acidic cystein rich glycoprotein; secreted acidic cysteine rich glycoprotein; Secreted acidic cystein-rich glycoprotein (osteonectin); secreted protein acidic and cysteine rich; secreted protein acidic and rich in cysteine; Secreted protein acidic and rich in cysteine (SPARC); secreted protein, acidic, cysteine-rich (osteonectin); secreted protein, acidic, cysteine-rich [osteonectin]; SPARC
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA