product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
eNOS Monoclonal Antibody (9D10)
catalog :
33-4600
quantity :
100 µg
price :
US 398.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
9D10
reactivity :
African green monkey, human, mouse, rat, bovine, pigs , domestic sheep, domestic rabbit
application :
western blot, ELISA, immunoprecipitation
more info or order :
Invitrogen product webpage
citations: 28
Published Application/Species/Sample/DilutionReference
  • western blot; human
Shapero K, Wylie Sears J, Levine R, Mayer J, Bischoff J. Reciprocal interactions between mitral valve endothelial and interstitial cells reduce endothelial-to-mesenchymal transition and myofibroblastic activation. J Mol Cell Cardiol. 2015;80:175-85 pubmed publisher
  • western blot; bovine
Salerno J, Ghosh D, Razdan R, Helms K, Brown C, McMurry J, et al. Endothelial nitric oxide synthase is regulated by ERK phosphorylation at Ser602. Biosci Rep. 2014;34: pubmed publisher
  • western blot; domestic sheep; 1:500; fig 5
Teng R, Du J, Xu H, Bakhutashvili I, Eis A, Shi Y, et al. Sepiapterin improves angiogenesis of pulmonary artery endothelial cells with in utero pulmonary hypertension by recoupling endothelial nitric oxide synthase. Am J Physiol Lung Cell Mol Physiol. 2011;301:L334-45 pubmed publisher
  • western blot; human; fig 4
Liu W, Zuo H, Chai B, Peng D, Fei Y, Lin J, et al. Role of tetraspanin CD151-?3/?6 integrin complex: Implication in angiogenesis CD151-integrin complex in angiogenesis. Int J Biochem Cell Biol. 2011;43:642-50 pubmed publisher
  • western blot; domestic sheep; fig 5
Teng R, Wu T, Bisig C, Eis A, Pritchard K, Konduri G. Nitrotyrosine impairs angiogenesis and uncouples eNOS activity of pulmonary artery endothelial cells isolated from developing sheep lungs. Pediatr Res. 2011;69:112-7 pubmed publisher
  • western blot; African green monkey; fig 3
Church J, Qian J, Kumar S, Black S, Venema R, Papapetropoulos A, et al. Inhibition of endothelial nitric oxide synthase by the lipid phosphatase PTEN. Vascul Pharmacol. 2010;52:191-8 pubmed publisher
  • western blot; pigs ; fig 5
Zuo H, Liu Z, Liu X, Yang J, Liu T, Wen S, et al. CD151 gene delivery after myocardial infarction promotes functional neovascularization and activates FAK signaling. Mol Med. 2009;15:307-15 pubmed publisher
  • immunoprecipitation; domestic sheep; fig 9
  • western blot; domestic sheep; fig 9
Chandrasekar I, Eis A, Konduri G. Betamethasone attenuates oxidant stress in endothelial cells from fetal lambs with persistent pulmonary hypertension. Pediatr Res. 2008;63:67-72 pubmed
  • immunoprecipitation; domestic sheep; fig 6
  • western blot; domestic sheep; fig 6
Konduri G, Bakhutashvili I, Eis A, Pritchard K. Oxidant stress from uncoupled nitric oxide synthase impairs vasodilation in fetal lambs with persistent pulmonary hypertension. Am J Physiol Heart Circ Physiol. 2007;292:H1812-20 pubmed publisher
  • western blot; bovine
Chen Y, Medhora M, Falck J, Pritchard K, Jacobs E. Mechanisms of activation of eNOS by 20-HETE and VEGF in bovine pulmonary artery endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2006;291:L378-85 pubmed
  • western blot; bovine; fig 3
Papapetropoulos A, Fulton D, Lin M, Fontana J, McCabe T, Zoellner S, et al. Vanadate is a potent activator of endothelial nitric-oxide synthase: evidence for the role of the serine/threonine kinase Akt and the 90-kDa heat shock protein. Mol Pharmacol. 2004;65:407-15 pubmed
  • western blot; mouse; 1:250; fig 5
Pritchard K, Ou J, Ou Z, Shi Y, Franciosi J, Signorino P, et al. Hypoxia-induced acute lung injury in murine models of sickle cell disease. Am J Physiol Lung Cell Mol Physiol. 2004;286:L705-14 pubmed
  • western blot; bovine; fig 1
Lin M, Fulton D, Babbitt R, Fleming I, Busse R, Pritchard K, et al. Phosphorylation of threonine 497 in endothelial nitric-oxide synthase coordinates the coupling of L-arginine metabolism to efficient nitric oxide production. J Biol Chem. 2003;278:44719-26 pubmed
  • western blot; domestic sheep
Konduri G, Ou J, Shi Y, Pritchard K. Decreased association of HSP90 impairs endothelial nitric oxide synthase in fetal lambs with persistent pulmonary hypertension. Am J Physiol Heart Circ Physiol. 2003;285:H204-11 pubmed
  • western blot; bovine; 1:1000
Ou J, Ou Z, McCarver D, Hines R, Oldham K, Ackerman A, et al. Trichloroethylene decreases heat shock protein 90 interactions with endothelial nitric oxide synthase: implications for endothelial cell proliferation. Toxicol Sci. 2003;73:90-7 pubmed
  • western blot; rat; fig 2
Koshida R, Ou J, Matsunaga T, Chilian W, Oldham K, Ackerman A, et al. Angiostatin: a negative regulator of endothelial-dependent vasodilation. Circulation. 2003;107:803-6 pubmed
  • immunoprecipitation; bovine; fig 1
  • western blot; bovine; fig 1
Ou J, Ou Z, Ackerman A, Oldham K, Pritchard K. Inhibition of heat shock protein 90 (hsp90) in proliferating endothelial cells uncouples endothelial nitric oxide synthase activity. Free Radic Biol Med. 2003;34:269-76 pubmed
  • western blot; bovine; 1:500
Akiyama M, Eguchi D, Weiler D, O BRIEN T, Kovesdi I, Scotland R, et al. Expression and function of recombinant S1179D endothelial nitric oxide synthase in canine cerebral arteries. Stroke. 2002;33:1071-6 pubmed
  • western blot; bovine; fig 1
Fulton D, Fontana J, Sowa G, Gratton J, Lin M, Li K, et al. Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in Golgi and plasma membrane defines the existence of two pools of active enzyme. J Biol Chem. 2002;277:4277-84 pubmed
  • immunoprecipitation; bovine; fig 3
  • western blot; bovine; 1:1000; fig 3
Pritchard K, Ackerman A, Gross E, Stepp D, Shi Y, Fontana J, et al. Heat shock protein 90 mediates the balance of nitric oxide and superoxide anion from endothelial nitric-oxide synthase. J Biol Chem. 2001;276:17621-4 pubmed
  • western blot; domestic rabbit; 1:1000; fig 2
Shi Y, Pritchard K, Holman P, Rafiee P, Griffith O, Kalyanaraman B, et al. Chronic myocardial hypoxia increases nitric oxide synthase and decreases caveolin-3. Free Radic Biol Med. 2000;29:695-703 pubmed
  • western blot; bovine
Gratton J, Fontana J, O Connor D, Garcia Cardena G, McCabe T, Sessa W. Reconstitution of an endothelial nitric-oxide synthase (eNOS), hsp90, and caveolin-1 complex in vitro. Evidence that hsp90 facilitates calmodulin stimulated displacement of eNOS from caveolin-1. J Biol Chem. 2000;275:22268-72 pubmed
  • western blot; human; fig 1
Sowa G, Liu J, Papapetropoulos A, Rex Haffner M, Hughes T, Sessa W. Trafficking of endothelial nitric-oxide synthase in living cells. Quantitative evidence supporting the role of palmitoylation as a kinetic trapping mechanism limiting membrane diffusion. J Biol Chem. 1999;274:22524-31 pubmed
  • western blot; bovine
Fulton D, Gratton J, McCabe T, Fontana J, Fujio Y, Walsh K, et al. Regulation of endothelium-derived nitric oxide production by the protein kinase Akt. Nature. 1999;399:597-601 pubmed
Ke X, Johnson H, Jing X, Michalkiewicz T, Huang Y, Lane R, et al. Persistent pulmonary hypertension alters the epigenetic characteristics of endothelial nitric oxide synthase gene in pulmonary artery endothelial cells in a fetal lamb model. Physiol Genomics. 2018;50:828-836 pubmed publisher
Fork C, Gu L, Hitzel J, Josipovic I, Hu J, SzeKa Wong M, et al. Epigenetic Regulation of Angiogenesis by JARID1B-Induced Repression of HOXA5. Arterioscler Thromb Vasc Biol. 2015;35:1645-52 pubmed publisher
Church J, Fulton D. Differences in eNOS activity because of subcellular localization are dictated by phosphorylation state rather than the local calcium environment. J Biol Chem. 2006;281:1477-88 pubmed
Bauer P, Fulton D, Boo Y, Sorescu G, Kemp B, Jo H, et al. Compensatory phosphorylation and protein-protein interactions revealed by loss of function and gain of function mutants of multiple serine phosphorylation sites in endothelial nitric-oxide synthase. J Biol Chem. 2003;278:14841-9 pubmed
product information
Product Type :
Antibody
Product Name :
eNOS Monoclonal Antibody (9D10)
Catalog # :
33-4600
Quantity :
100 µg
Price :
US 398.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Bovine
Applications :
ELISA: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent
Species :
Bovine
Clone :
9D10
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
Immunogen :
Purified bovine recombinant eNOS protein
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent
Aliases :
2310065A03Rik; cNOS; Constitutive NOS; Ecnos; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; endothelial nitric oxide synthase NOS3; endothelial NOS; eNos; e-NOS; nitric oxide synthase; nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3, endothelial cell; nitric oxide synthase, endothelial; NOS; NOS type III; Nos3; Nos-3; NOSIII; OTTHUMP00000213183
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA