Connexin 50 Monoclonal Antibody (C6) | Invitrogen 33-4300 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Connexin 50 Monoclonal Antibody (C6)

catalog :

33-4300

quantity :

100 µg

price :

US 398.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat, domestic sheep, domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; rat; 1:200; fig 1 western blot; rat; 1:500; fig 1	Rodríguez Jiménez F, Alastrue A, Stojkovic M, Erceg S, Moreno Manzano V. Connexin 50 modulates Sox2 expression in spinal-cord-derived ependymal stem/progenitor cells. Cell Tissue Res. 2016;365:295-307 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000	Bolte P, Herrling R, Dorgau B, Schultz K, Feigenspan A, Weiler R, et al. Expression and Localization of Connexins in the Outer Retina of the Mouse. J Mol Neurosci. 2016;58:178-92 pubmed publisher
immunocytochemistry; mouse; 0.7 ug/ml immunohistochemistry; mouse; 0.5 ug/ml western blot; mouse; 0.2 ug/ml	Dorgau B, Herrling R, Schultz K, Greb H, Segelken J, StrÃ¶h S, et al. Connexin50 couples axon terminals of mouse horizontal cells by homotypic gap junctions. J Comp Neurol. 2015;523:2062-81 pubmed publisher
western blot; mouse; fig 2	Das S, Wang H, Molina S, Martinez Wittinghan F, Jena S, Bossmann L, et al. PKC?, role in lens differentiation and gap junction coupling. Curr Eye Res. 2011;36:620-31 pubmed publisher
immunohistochemistry - paraffin section; human; 1:1000; fig 1	Willenberg H, Schott M, Saeger W, Tries A, Scherbaum W, Bornstein S. Expression of connexins in chromaffin cells of normal human adrenals and in benign and malignant pheochromocytomas. Ann N Y Acad Sci. 2006;1073:578-83 pubmed
immunohistochemistry - frozen section; domestic rabbit; 5-10 ug/ml	Zahs K, Ceelen P. Gap junctional coupling and connexin immunoreactivity in rabbit retinal glia. Vis Neurosci. 2006;23:1-10 pubmed
	Fabbiani G, Reali C, Valentin Kahan A, Rehermann M, Fagetti J, Falco M, et al. Connexin Signaling Is Involved in the Reactivation of a Latent Stem Cell Niche after Spinal Cord Injury. J Neurosci. 2020;40:2246-2258 pubmed publisher
	Pelletier R, Akpovi C, Chen L, Vitale M. Cholesterol metabolism and Cx43, Cx46, Cx50 gap junction protein expression and localisation in normal and diabetic and obese ob/ob and db/db mouse testes. Am J Physiol Endocrinol Metab. 2017;:ajpendo.00215.2017 pubmed publisher
	Shahidullah M, Mandal A, Beimgraben C, Delamere N. Hyposmotic stress causes ATP release and stimulates Na,K-ATPase activity in porcine lens. J Cell Physiol. 2012;227:1428-37 pubmed publisher
	Zampighi G, Planells A, Lin D, Takemoto D. Regulation of lens cell-to-cell communication by activation of PKCgamma and disassembly of Cx50 channels. Invest Ophthalmol Vis Sci. 2005;46:3247-55 pubmed
	Lin D, Lobell S, Jewell A, Takemoto D. Differential phosphorylation of connexin46 and connexin50 by H2O2 activation of protein kinase Cgamma. Mol Vis. 2004;10:688-95 pubmed
	Klepeis V, Cornell Bell A, Trinkaus Randall V. Growth factors but not gap junctions play a role in injury-induced Ca2+ waves in epithelial cells. J Cell Sci. 2001;114:4185-95 pubmed

product information

Product Type :

Antibody

Product Name :

Connexin 50 Monoclonal Antibody (C6)

Catalog # :

33-4300

Quantity :

100 µg

Price :

US 398.00

Clonality :

Monoclonal

Purity :

Size-exclusion chromatography

Host :

Mouse

Reactivity :

Human, Mouse, Ovine, Rat

Applications :

ELISA: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Species :

Human, Mouse, Ovine, Rat

Clone :

Isotype :

IgM, kappa

Storage :

-20°C

Description :

This gene encodes a transmembrane connexin protein that is necessary for lens growth and maturation of lens fiber cells. The encoded protein is a component of gap junction channels and functions in a calcium and pH-dependent manner. Mutations in this gene have been associated with zonular pulverulent cataracts, nuclear progressive cataracts, and cataract-microcornea syndrome.

Immunogen :

This antibody was prepared against urea/alkali stripped sheep lens membranes.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Aliases :

Aey5; alpha 8 connexin; CAE; CAE1; cell surface glycoprotein; Cnx50; connexin 50; connexin-49; Connexin-50; CTRCT1; cx49; CX50; CZP1; gap junction alpha 8; gap junction alpha-8 protein; gap junction membrane channel protein alpha 8; gap junction membrane channel protein alpha-8; gap junction protein alpha 8; gap junction protein alpha 8 50kDa; gap junction protein, alpha 8; gap junction protein, alpha 8, 50kDa; GJA8; Lens fiber protein MP70; lens intrinsic membrane protein MP70; lens opacity 10; Lop10; MP38; MP64; MP70

more info or order :

Invitrogen product webpage