Cyclin D1 Monoclonal Antibody (AM29) | Invitrogen 33-3500 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cyclin D1 Monoclonal Antibody (AM29)

catalog :

33-3500

quantity :

100 µg

price :

US 454.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AM29

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 41

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; tbl 2	Abrosimov A, Dvinskikh N, Sidorin A. Cells of Benign and Borderline Thyroid Tumor Express Malignancy Markers. Bull Exp Biol Med. 2016;160:698-701 pubmed publisher
western blot; human	Fausti F, Di Agostino S, Cioce M, Bielli P, Sette C, Pandolfi P, et al. ATM kinase enables the functional axis of YAP, PML and p53 to ameliorate loss of Werner protein-mediated oncogenic senescence. Cell Death Differ. 2013;20:1498-509 pubmed publisher
immunohistochemistry - paraffin section; human; fig 2	Lara P, Lloret M, Valenciano A, Clavo B, Pinar B, Rey A, et al. Plasminogen activator inhibitor-1 (PAI-1) expression in relation to hypoxia and oncoproteins in clinical cervical tumors. Strahlenther Onkol. 2012;188:1139-45 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:500; fig 2	Deckelbaum R, Holmes G, Zhao Z, Tong C, Basilico C, Loomis C. Regulation of cranial morphogenesis and cell fate at the neural crest-mesoderm boundary by engrailed 1. Development. 2012;139:1346-58 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50	Thieblemont C, Rolland D, Baseggio L, Felman P, Gazzo S, Callet Bauchu E, et al. Comprehensive analysis of GST-pi expression in B-cell lymphomas: Correlation with histological subtypes and survival. Leuk Lymphoma. 2008;49:1403-6 pubmed publisher
immunohistochemistry - paraffin section; human; fig 1	Mottolese M, Orlandi G, Sperduti I, Merola R, Buglioni S, Di Benedetto A, et al. Bio-pathologic characteristics related to chromosome 11 aneusomy and cyclin D1 gene status in surgically resected stage I and II breast cancer: Identification of an adverse prognostic profile. Am J Surg Pathol. 2007;31:247-54 pubmed
western blot; human; 1.1 ug/ml; fig 4	Jones A, Jonsson A, Aro H. Neisseria gonorrhoeae infection causes a G1 arrest in human epithelial cells. FASEB J. 2007;21:345-55 pubmed
immunohistochemistry - paraffin section; human; 1:100	Horn L, Richter C, Einenkel J, Tannapfel A, Liebert U, Leo C. p16, p14, p53, cyclin D1, and steroid hormone receptor expression and human papillomaviruses analysis in primary squamous cell carcinoma of the endometrium. Ann Diagn Pathol. 2006;10:193-6 pubmed
immunohistochemistry - frozen section; mouse	Akimoto M, Cheng H, Zhu D, Brzezinski J, Khanna R, Filippova E, et al. Targeting of GFP to newborn rods by Nrl promoter and temporal expression profiling of flow-sorted photoreceptors. Proc Natl Acad Sci U S A. 2006;103:3890-5 pubmed
immunohistochemistry - paraffin section; human; fig 2	Mikami T, Yoshida T, Shiraishi H, Tokuyama W, Motoori T, Okayasu I. Bottom-up cell proliferation with cyclin A and p27Kip1 expression in ulcerative colitis-associated dysplasia. Pathol Int. 2006;56:10-6 pubmed
immunohistochemistry; human; tbl 1	Takahashi Y, Fukusato T, Aita K, Toida S, Fukushima J, Imamura T, et al. Solid pseudopapillary tumor of the pancreas with metastases to the lung and liver. Pathol Int. 2005;55:792-6 pubmed
immunohistochemistry; human	Uziel T, Zindy F, Xie S, Lee Y, Forget A, Magdaleno S, et al. The tumor suppressors Ink4c and p53 collaborate independently with Patched to suppress medulloblastoma formation. Genes Dev. 2005;19:2656-67 pubmed
immunohistochemistry - paraffin section; human; 1:15	Konoplev S, Medeiros L, Bueso Ramos C, Jorgensen J, Lin P. Immunophenotypic profile of lymphoplasmacytic lymphoma/Waldenström macroglobulinemia. Am J Clin Pathol. 2005;124:414-20 pubmed
immunohistochemistry - paraffin section; mouse; 1:500 western blot; mouse	Misikangas M, Pajari A, Paivarinta E, Mutanen M. Promotion of adenoma growth by dietary inulin is associated with increase in cyclin D1 and decrease in adhesion proteins in Min/+ mice mucosa. J Nutr Biochem. 2005;16:402-9 pubmed
immunohistochemistry - paraffin section; human; fig 3	Kamoshida S, Matsuoka H, Shiogama K, Matsuyama A, Shimomura R, Inada K, et al. Immunohistochemical analysis of thymidylate synthase, p16(INK4a), cyclin-dependent kinase 4 and cyclin D1 in colorectal cancers receiving preoperative chemotherapy: significance of p16(INK4a)-mediated cellular arrest as an indicator of chemosensitivi. Pathol Int. 2004;54:564-75 pubmed
immunohistochemistry - paraffin section; human; 1:800; fig 5	Pantanowitz L, Dezube B, Pinkus G, Tahan S. Histological characterization of regression in acquired immunodeficiency syndrome-related Kaposi's sarcoma. J Cutan Pathol. 2004;31:26-34 pubmed
immunohistochemistry - paraffin section; human	Adachi A, Tamaru J, Kaneko K, Kuroda H, Miura I, Kojima T, et al. No evidence of a correlation between BCL10 expression and API2-MALT1 gene rearrangement in ocular adnexal MALT lymphoma. Pathol Int. 2004;54:16-25 pubmed
western blot; mouse; 1:500; fig 4	Haegele L, Ingold B, Naumann H, Tabatabai G, Ledermann B, Brandner S. Wnt signalling inhibits neural differentiation of embryonic stem cells by controlling bone morphogenetic protein expression. Mol Cell Neurosci. 2003;24:696-708 pubmed
immunohistochemistry - paraffin section; human	Yamazaki K, Hanami K, Nagao T, Asoh A, Sugano I, Ishida Y. Increased cyclin D1 expression in cancer of the ampulla of Vater: relevance to nuclear beta catenin accumulation and k-ras gene mutation. Mol Pathol. 2003;56:336-41 pubmed
immunohistochemistry - paraffin section; human	Schlette E, Fu K, Medeiros L. CD23 expression in mantle cell lymphoma: clinicopathologic features of 18 cases. Am J Clin Pathol. 2003;120:760-6 pubmed
immunohistochemistry - paraffin section; human; 1:400; fig 1	Beasley M, Lantuejoul S, Abbondanzo S, Chu W, Hasleton P, Travis W, et al. The P16/cyclin D1/Rb pathway in neuroendocrine tumors of the lung. Hum Pathol. 2003;34:136-42 pubmed
immunohistochemistry - paraffin section; human	Peiro G, Diebold J, Löhrs U. CAS (cellular apoptosis susceptibility) gene expression in ovarian carcinoma: Correlation with 20q13.2 copy number and cyclin D1, p53, and Rb protein expression. Am J Clin Pathol. 2002;118:922-9 pubmed
immunohistochemistry - paraffin section; human; 1:20	Xu Y, McKenna R, Asplund S, Kroft S. Comparison of immunophenotypes of small B-cell neoplasms in primary lymph node and concurrent blood or marrow samples. Am J Clin Pathol. 2002;118:758-64 pubmed
immunohistochemistry - paraffin section; human	Gloghini A, Gaidano G, Larocca L, Pierconti F, Cingolani A, Dal Maso L, et al. Expression of cyclin-dependent kinase inhibitor p27(Kip1) in AIDS-related diffuse large-cell lymphomas is associated with Epstein-Barr virus-encoded latent membrane protein 1. Am J Pathol. 2002;161:163-71 pubmed
western blot; human; fig 5	Montagnoli A, Bosotti R, Villa F, Rialland M, Brotherton D, Mercurio C, et al. Drf1, a novel regulatory subunit for human Cdc7 kinase. EMBO J. 2002;21:3171-81 pubmed
immunohistochemistry - paraffin section; human; fig 5	Degan M, Doliana R, Gloghini A, Di Francia R, Aldinucci D, Mazzocut Zecchin L, et al. A novel bcl-1/JH breakpoint from a patient affected by mantle cell lymphoma extends the major translocation cluster. J Pathol. 2002;197:256-63 pubmed
immunohistochemistry - paraffin section; human; 1:20	Xu Y, McKenna R, Kroft S. Assessment of CD10 in the diagnosis of small B-cell lymphomas: a multiparameter flow cytometric study. Am J Clin Pathol. 2002;117:291-300 pubmed
immunohistochemistry - paraffin section; human; 1:15	Schlette E, Lai R, Onciu M, Doherty D, Bueso Ramos C, Medeiros L. Leukemic mantle cell lymphoma: clinical and pathologic spectrum of twenty-three cases. Mod Pathol. 2001;14:1133-40 pubmed
western blot; human; fig 2a	Castoria G, Migliaccio A, Bilancio A, Di Domenico M, de Falco A, Lombardi M, et al. PI3-kinase in concert with Src promotes the S-phase entry of oestradiol-stimulated MCF-7 cells. EMBO J. 2001;20:6050-9 pubmed
immunohistochemistry - paraffin section; human; 1:75	Kroft S, Dawson D, McKenna R. Large cell lymphoma transformation of chronic lymphocytic leukemia/small lymphocytic lymphoma. A flow cytometric analysis of seven cases. Am J Clin Pathol. 2001;115:385-95 pubmed
immunohistochemistry - paraffin section; human	Kroft S, Howard M, Picker L, Ansari M, Aquino D, McKenna R. De novo CD5+ diffuse large B-cell lymphomas. A heterogeneous group containing an unusual form of splenic lymphoma. Am J Clin Pathol. 2000;114:523-33 pubmed
immunocytochemistry; human	Carbone A, Gloghini A, Bontempo D, Monini P, Tirelli U, Volpe R, et al. Proliferation in HHV-8-positive primary effusion lymphomas is associated with expression of HHV-8 cyclin but independent of p27(kip1). Am J Pathol. 2000;156:1209-15 pubmed
immunohistochemistry - paraffin section; mouse; fig 2	Pérez de Castro I, Malumbres M, Santos J, Pellicer A, Fernandez Piqueras J. Cooperative alterations of Rb pathway regulators in mouse primary T cell lymphomas. Carcinogenesis. 1999;20:1675-82 pubmed
	Enomoto K, Sato F, Tamagawa S, Gunduz M, Onoda N, Uchino S, et al. A novel therapeutic approach for anaplastic thyroid cancer through inhibition of LAT1. Sci Rep. 2019;9:14616 pubmed publisher
	Roy S, Hooiveld G, Seehawer M, Caruso S, Heinzmann F, Schneider A, et al. microRNA 193a-5p Regulates Levels of Nucleolar- and Spindle-Associated Protein 1 to Suppress Hepatocarcinogenesis. Gastroenterology. 2018;155:1951-1966.e26 pubmed publisher
	Bilancio A, Bontempo P, Di Donato M, Conte M, Giovannelli P, Altucci L, et al. Bisphenol A induces cell cycle arrest in primary and prostate cancer cells through EGFR/ERK/p53 signaling pathway activation. Oncotarget. 2017;8:115620-115631 pubmed publisher
	Maudet C, Mano M, Sunkavalli U, Sharan M, Giacca M, Förstner K, et al. Functional high-throughput screening identifies the miR-15 microRNA family as cellular restriction factors for Salmonella infection. Nat Commun. 2014;5:4718 pubmed publisher
	Feng M, Gao W, Wang R, Chen W, Man Y, Figg W, et al. Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proc Natl Acad Sci U S A. 2013;110:E1083-91 pubmed publisher
	Logan P, Steiner M, Ponnampalam A, Mitchell M. Cell cycle regulation of human endometrial stromal cells during decidualization. Reprod Sci. 2012;19:883-94 pubmed publisher
	Jacquemier J, Ginestier C, Rougemont J, Bardou V, Charafe Jauffret E, Geneix J, et al. Protein expression profiling identifies subclasses of breast cancer and predicts prognosis. Cancer Res. 2005;65:767-79 pubmed
	Yang W, Bancroft L, Nicholas C, Lozonschi I, Augenlicht L. Targeted inactivation of p27kip1 is sufficient for large and small intestinal tumorigenesis in the mouse, which can be augmented by a Western-style high-risk diet. Cancer Res. 2003;63:4990-6 pubmed

product information

Product Type :

Antibody

Product Name :

Cyclin D1 Monoclonal Antibody (AM29)

Catalog # :

33-3500

Quantity :

100 µg

Price :

US 454.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

ELISA: 0.1-1.0 µg/mL, Immunohistochemistry: 2-10 µg/mL, Immunoprecipitation: 5 µg, Western Blot: 0.3-1.0 µg/mL

Species :

Human, Mouse

Clone :

AM29

Isotype :

IgG2a, kappa

Storage :

-20°C

Description :

Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation. In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11. Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Without growth factor-mediated stimulation, Cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. The Cyclin D1 protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The Cyclin D1 protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Cyclin D1 has been successfully employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities.

Immunogen :

Recombinant full-length human cyclin D1 protein.

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.1-1.0 µg/mL, Immunohistochemistry: 2-10 µg/mL, Immunoprecipitation: 5 µg, Western Blot: 0.3-1.0 µg/mL

Aliases :

AI327039; B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL1; BCL-1; BCL-1 oncogene; CCND1; CCND1 protein; cD1; cDNA; CycD1; cyclin D1; Cyl-1; D11S287E; FLJ93625; G1/S-specific cyclin-D1; mRNA; PRAD1; PRAD-1; PRAD1 oncogene; U21B31

more info or order :

Invitrogen product webpage