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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Lamin B1 Monoclonal Antibody (L-5)
catalog :
33-2000
quantity :
100 µg
price :
US 398.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
L-5
reactivity :
human, mouse, rat, dogs, chicken
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 56
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 5f
  • western blot; human; loading ...; fig s6a
Sugiyama T, Murao N, Kadowaki H, Takao K, Miyakawa T, Matsushita Y, et al. ERAD components Derlin-1 and Derlin-2 are essential for postnatal brain development and motor function. iScience. 2021;24:102758 pubmed publisher
  • western blot; mouse; fig 6a
Ma S, Wan X, Deng Z, Shi L, Hao C, Zhou Z, et al. Epigenetic regulator CXXC5 recruits DNA demethylase Tet2 to regulate TLR7/9-elicited IFN response in pDCs. J Exp Med. 2017;214:1471-1491 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 6
Walker E, Jensen L, Croft S, Wei K, Fulcher A, Jans D, et al. Rhinovirus 16 2A Protease Affects Nuclear Localization of 3CD during Infection. J Virol. 2016;90:11032-11042 pubmed
  • western blot; dogs; 1:500; loading ...; fig 6a
Wetzel F, Mittag S, Cano Cortina M, Wagner T, Kramer O, Niedenthal R, et al. SUMOylation regulates the intracellular fate of ZO-2. Cell Mol Life Sci. 2017;74:373-392 pubmed publisher
  • western blot; mouse; fig s5
Fagnocchi L, Cherubini A, Hatsuda H, Fasciani A, Mazzoleni S, Poli V, et al. A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity. Nat Commun. 2016;7:11903 pubmed publisher
  • immunocytochemistry; human; fig 2a
De Marco G, Lomartire A, Calvo A, Risso A, De Luca E, Mostert M, et al. Monocytes of patients with amyotrophic lateral sclerosis linked to gene mutations display altered TDP-43 subcellular distribution. Neuropathol Appl Neurobiol. 2017;43:133-153 pubmed publisher
  • western blot; mouse; 1:2000; loading ...
Uruno A, Yagishita Y, Katsuoka F, Kitajima Y, Nunomiya A, Nagatomi R, et al. Nrf2-Mediated Regulation of Skeletal Muscle Glycogen Metabolism. Mol Cell Biol. 2016;36:1655-72 pubmed publisher
  • western blot; human; 1:1000; fig 2
Rhee M, Lee S, Kim J, Ham D, Park H, Yang H, et al. Preadipocyte factor 1 induces pancreatic ductal cell differentiation into insulin-producing cells. Sci Rep. 2016;6:23960 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 1
  • western blot; mouse; fig 1
Rolyan H, Tyurina Y, Hernandez M, Amoscato A, Sparvero L, Nmezi B, et al. Defects of Lipid Synthesis Are Linked to the Age-Dependent Demyelination Caused by Lamin B1 Overexpression. J Neurosci. 2015;35:12002-17 pubmed publisher
  • western blot; mouse
He L, Zang A, Du M, Ma D, Yuan C, Zhou C, et al. mTOR regulates TLR-induced c-fos and Th1 responses to HBV and HCV vaccines. Virol Sin. 2015;30:174-89 pubmed publisher
  • western blot; human; fig 2
Korotkevych N, Labyntsev A, Kolybo D, Komisarenko S. The Soluble Heparin-Binding EGF-Like Growth Factor Stimulates EGF Receptor Trafficking to the Nucleus. PLoS ONE. 2015;10:e0127887 pubmed publisher
  • western blot; mouse
Galvagni F, Lentucci C, Neri F, Dettori D, De Clemente C, Orlandini M, et al. Snai1 promotes ESC exit from the pluripotency by direct repression of self-renewal genes. Stem Cells. 2015;33:742-50 pubmed publisher
  • western blot; human
Kim H, Li A, Ahn S, Song H, Zhang W. Inositol Polyphosphate-5-Phosphatase F (INPP5F) inhibits STAT3 activity and suppresses gliomas tumorigenicity. Sci Rep. 2014;4:7330 pubmed publisher
  • western blot; human
Trembley J, Unger G, Korman V, Abedin M, Nacusi L, Vogel R, et al. Tenfibgen ligand nanoencapsulation delivers bi-functional anti-CK2 RNAi oligomer to key sites for prostate cancer targeting using human xenograft tumors in mice. PLoS ONE. 2014;9:e109970 pubmed publisher
  • western blot; rat; 1:500
Tavares E, Maldonado R, Miñano F. Immunoneutralization of endogenous aminoprocalcitonin attenuates sepsis-induced acute lung injury and mortality in rats. Am J Pathol. 2014;184:3069-83 pubmed publisher
  • western blot; mouse; fig 2
Li M, Pettitt S, Eckert S, Ning Z, Rice S, Cadinanos J, et al. The piggyBac transposon displays local and distant reintegration preferences and can cause mutations at noncanonical integration sites. Mol Cell Biol. 2013;33:1317-30 pubmed publisher
  • western blot; human; fig 3
Hassan M, Maeda Y, Taipaleenmaki H, Zhang W, Jafferji M, Gordon J, et al. miR-218 directs a Wnt signaling circuit to promote differentiation of osteoblasts and osteomimicry of metastatic cancer cells. J Biol Chem. 2012;287:42084-92 pubmed publisher
  • western blot; rat; fig 9
Inoue K, Matsui I, Hamano T, Fujii N, Shimomura A, Nakano C, et al. Maxacalcitol ameliorates tubulointerstitial fibrosis in obstructed kidneys by recruiting PPM1A/VDR complex to pSmad3. Lab Invest. 2012;92:1686-97 pubmed publisher
  • western blot; human; 1:100; fig 4
Neumann L, Markaki Y, Mladenov E, Hoffmann D, Buiting K, Horsthemke B. The imprinted NPAP1/C15orf2 gene in the Prader-Willi syndrome region encodes a nuclear pore complex associated protein. Hum Mol Genet. 2012;21:4038-48 pubmed publisher
  • immunohistochemistry; rat; 1:100; fig 1
  • western blot; rat; 1:100; fig 5
Wakabayashi T, Mori T, Hirahara Y, Koike T, Kubota Y, Takamori Y, et al. Nuclear lamins are differentially expressed in retinal neurons of the adult rat retina. Histochem Cell Biol. 2011;136:427-36 pubmed publisher
  • western blot; human; fig 3
Abel E, Bubel J, Simper M, Powell L, McClellan S, Andreeff M, et al. Protection against 2-chloroethyl ethyl sulfide (CEES)-induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway. Toxicol Appl Pharmacol. 2011;255:176-83 pubmed publisher
  • western blot; human; fig 1
De Marco G, Lupino E, Calvo A, Moglia C, Buccinnà B, Grifoni S, et al. Cytoplasmic accumulation of TDP-43 in circulating lymphomonocytes of ALS patients with and without TARDBP mutations. Acta Neuropathol. 2011;121:611-22 pubmed publisher
  • western blot; human; fig 8
Lupino E, Buccinnà B, Ramondetti C, Lomartire A, De Marco G, Ricotti E, et al. In CD28-costimulated human naïve CD4+ T cells, I-?B kinase controls the expression of cell cycle regulatory proteins via interleukin-2-independent mechanisms. Immunology. 2010;131:231-41 pubmed publisher
  • western blot; mouse
Kim K, Yoon Y, Lee H, Yoon S, Kim S, Shin S, et al. Enhanced hypothalamic leptin signaling in mice lacking dopamine D2 receptors. J Biol Chem. 2010;285:8905-17 pubmed publisher
  • western blot; human; fig 5
Honma K, Tsuzuki S, Nakagawa M, Tagawa H, Nakamura S, Morishima Y, et al. TNFAIP3/A20 functions as a novel tumor suppressor gene in several subtypes of non-Hodgkin lymphomas. Blood. 2009;114:2467-75 pubmed publisher
  • western blot; human; fig 1
Giordana M, Piccinini M, Grifoni S, De Marco G, Vercellino M, Magistrello M, et al. TDP-43 redistribution is an early event in sporadic amyotrophic lateral sclerosis. Brain Pathol. 2010;20:351-60 pubmed publisher
  • western blot; human; 1:100; fig 4
Joung E, Li M, Lee H, Somparn N, Jung Y, Na H, et al. Capsaicin induces heme oxygenase-1 expression in HepG2 cells via activation of PI3K-Nrf2 signaling: NAD(P)H:quinone oxidoreductase as a potential target. Antioxid Redox Signal. 2007;9:2087-98 pubmed
  • western blot; rat
Li M, Jang J, Na H, Cha Y, Surh Y. Carbon monoxide produced by heme oxygenase-1 in response to nitrosative stress induces expression of glutamate-cysteine ligase in PC12 cells via activation of phosphatidylinositol 3-kinase and Nrf2 signaling. J Biol Chem. 2007;282:28577-86 pubmed
  • western blot; mouse; fig 4
Niida H, Katsuno Y, Banerjee B, Hande M, Nakanishi M. Specific role of Chk1 phosphorylations in cell survival and checkpoint activation. Mol Cell Biol. 2007;27:2572-81 pubmed
  • western blot; mouse; 1:1000; fig 1
Ghanem L, Steinman R. A proapoptotic function of p21 in differentiating granulocytes. Leuk Res. 2005;29:1315-23 pubmed
  • western blot; dogs; 1:500; fig 1
Jaramillo B, Ponce A, Moreno J, Betanzos A, Huerta M, Lopez Bayghen E, et al. Characterization of the tight junction protein ZO-2 localized at the nucleus of epithelial cells. Exp Cell Res. 2004;297:247-58 pubmed
  • western blot; dogs; 1:500; fig 8
Betanzos A, Huerta M, Lopez Bayghen E, Azuara E, Amerena J, Gonzalez Mariscal L. The tight junction protein ZO-2 associates with Jun, Fos and C/EBP transcription factors in epithelial cells. Exp Cell Res. 2004;292:51-66 pubmed
  • immunohistochemistry - paraffin section; human; 1:1000; fig 2C
  • western blot; human; fig 1
Oguchi M, Sagara J, Matsumoto K, Saida T, Taniguchi S. Expression of lamins depends on epidermal differentiation and transformation. Br J Dermatol. 2002;147:853-8 pubmed
Perrigue P, Rakoczy M, Pawlicka K, Belter A, Giel Pietraszuk M, Naskret Barciszewska M, et al. Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts. Cancers (Basel). 2020;12: pubmed publisher
Fassmannová D, Sedlák F, Sedlacek J, Spicka I, Grantz Sasková K. Nelfinavir Inhibits the TCF11/Nrf1-Mediated Proteasome Recovery Pathway in Multiple Myeloma. Cancers (Basel). 2020;12: pubmed publisher
Amaya E, Alarcón L, Martín Tapia D, Cuellar Perez F, Cano Cortina M, Ortega Olvera J, et al. Activation of the Ca2+ sensing receptor and the PKC/WNK4 downstream signaling cascade induces incorporation of ZO-2 to tight junctions and its separation from 14-3-3. Mol Biol Cell. 2019;30:2377-2398 pubmed publisher
Kim W, An H, Kim J, Gwon M, Gu H, Jeon M, et al. Anti-Inflammatory Effect of Melittin on Porphyromonas Gingivalis LPS-Stimulated Human Keratinocytes. Molecules. 2018;23: pubmed publisher
Maclean K, Jiang H, Aivazidis S, Kim E, Shearn C, Harris P, et al. Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism. FASEB J. 2018;32:1265-1280 pubmed publisher
Kim W, An H, Kim J, Gwon M, Gu H, Park J, et al. Bee Venom Inhibits Porphyromonas gingivalis Lipopolysaccharides-Induced Pro-Inflammatory Cytokines through Suppression of NF-κB and AP-1 Signaling Pathways. Molecules. 2016;21: pubmed
Takahara T, Matsuo K, Seto M, Nakamura S, Tsuzuki S. Synergistic activity of Card11 mutant and Bcl6 in the development of diffuse large B-cell lymphoma in a mouse model. Cancer Sci. 2016;107:1572-1580 pubmed publisher
Shah V, Aiken C. Cell Fractionation and Quantitative Analysis of HIV-1 Reverse Transcription in Target Cells. Bio Protoc. 2013;3: pubmed
Chou T, Chuang Y, Hsieh W, Chang P, Liu H, Mo S, et al. Tumour suppressor death-associated protein kinase targets cytoplasmic HIF-1? for Th17 suppression. Nat Commun. 2016;7:11904 pubmed publisher
Goretsky T, Bradford E, Ryu H, Tahir M, Moyer M, Gao T, et al. A Cytosolic Multiprotein Complex Containing p85α Is Required for β-Catenin Activation in Colitis and Colitis-associated Cancer. J Biol Chem. 2016;291:4166-77 pubmed publisher
Majkut S, Idema T, Swift J, Krieger C, Liu A, Discher D. Heart-specific stiffening in early embryos parallels matrix and myosin expression to optimize beating. Curr Biol. 2013;23:2434-9 pubmed publisher
Hinoi E, Nakamura Y, Takada S, Fujita H, Iezaki T, Hashizume S, et al. Growth differentiation factor-5 promotes brown adipogenesis in systemic energy expenditure. Diabetes. 2014;63:162-75 pubmed publisher
Quirós M, Alarcón L, Ponce A, Giannakouros T, Gonzalez Mariscal L. The intracellular fate of zonula occludens 2 is regulated by the phosphorylation of SR repeats and the phosphorylation/O-GlcNAcylation of S257. Mol Biol Cell. 2013;24:2528-43 pubmed publisher
Chort A, Alves S, Marinello M, Dufresnois B, Dornbierer J, Tesson C, et al. Interferon ? induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice. Brain. 2013;136:1732-45 pubmed publisher
Tsuchiya Y, Morita T, Kim M, Iemura S, Natsume T, Yamamoto M, et al. Dual regulation of the transcriptional activity of Nrf1 by ?-TrCP- and Hrd1-dependent degradation mechanisms. Mol Cell Biol. 2011;31:4500-12 pubmed publisher
Trembley J, Unger G, Tobolt D, Korman V, Wang G, Ahmad K, et al. Systemic administration of antisense oligonucleotides simultaneously targeting CK2? and ?' subunits reduces orthotopic xenograft prostate tumors in mice. Mol Cell Biochem. 2011;356:21-35 pubmed publisher
Li X, Liang D, Lin X, Robertson E, Lan K. Kaposi's sarcoma-associated herpesvirus-encoded latency-associated nuclear antigen reduces interleukin-8 expression in endothelial cells and impairs neutrophil chemotaxis by degrading nuclear p65. J Virol. 2011;85:8606-15 pubmed publisher
Fulcher A, Roth D, Fatima S, Alvisi G, Jans D. The BRCA-1 binding protein BRAP2 is a novel, negative regulator of nuclear import of viral proteins, dependent on phosphorylation flanking the nuclear localization signal. FASEB J. 2010;24:1454-66 pubmed publisher
Arts J, King P, Marien A, Floren W, Beliën A, Janssen L, et al. JNJ-26481585, a novel "second-generation" oral histone deacetylase inhibitor, shows broad-spectrum preclinical antitumoral activity. Clin Cancer Res. 2009;15:6841-51 pubmed publisher
Civelek M, Manduchi E, Riley R, Stoeckert C, Davies P. Chronic endoplasmic reticulum stress activates unfolded protein response in arterial endothelium in regions of susceptibility to atherosclerosis. Circ Res. 2009;105:453-61 pubmed publisher
Chandraskeharan K, Martin P. Embryonic overexpression of Galgt2 inhibits skeletal muscle growth via activation of myostatin signaling. Muscle Nerve. 2009;39:25-41 pubmed publisher
Vradii D, Zaidi S, Lian J, Van Wijnen A, Stein J, Stein G. Point mutation in AML1 disrupts subnuclear targeting, prevents myeloid differentiation, and effects a transformation-like phenotype. Proc Natl Acad Sci U S A. 2005;102:7174-9 pubmed
Zhang L, Shimizu S, Tsujimoto Y. Two distinct Fas-activated signaling pathways revealed by an antitumor drug D609. Oncogene. 2005;24:2954-62 pubmed
product information
Product Type :
Antibody
Product Name :
Lamin B1 Monoclonal Antibody (L-5)
Catalog # :
33-2000
Quantity :
100 µg
Price :
US 398.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Chicken, Human, Mouse, Rat
Applications :
ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 5-10 µg/mL, Immunoprecipitation: 2.5-5 µg, Western Blot: 2 µg/mL
Species :
Chicken, Human, Mouse, Rat
Clone :
L-5
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Defects in the LMNB1 gene can cause Leukodystrophy, demyelinating, autosomal dominant, adult-onset (ADLD).
Immunogen :
Extracts from chicken nuclei
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 5-10 µg/mL, Immunoprecipitation: 2.5-5 µg, Western Blot: 2 µg/mL
Aliases :
0710005A05Rik; ADLD; C-flanking peptide of NPY; CPON; fc06g01; I79_003938; lamin B1; lamin B1 L homeolog; lamin B1 protein; lamin-b; lamin-B1; Lamin-L(I); LMN; LMN2; LMNB; LMNB1; lmnb1 protein; lmnb1.L; MGC111419; Neuropeptide tyrosine; Neuropeptide Y; NPY; NPY02; prepro-neuropeptide Y; pro-neuropeptide Y; PYY4; RATNPY; RATNPY02; unnamed protein product; wu:fc06g01; XELAEV_18007951mg
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA