CDK1 Monoclonal Antibody (A17) | Invitrogen 33-1800 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CDK1 Monoclonal Antibody (A17)

catalog :

33-1800

quantity :

200 µg

price :

US 456.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A17

reactivity :

human, mouse, rat, chicken, Xenopus laevis

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:1000; fig s2	Kim A, Joseph S, Khan A, Epstein C, Sobel R, Huang T. Enhanced expression of mitochondrial superoxide dismutase leads to prolonged in vivo cell cycle progression and up-regulation of mitochondrial thioredoxin. Free Radic Biol Med. 2010;48:1501-12 pubmed publisher
	Jeon S, Yoon Y, Kim H, Han J, Lee K, Seol J, et al. Ablation of CRBN induces loss of type I collagen and SCH in mouse skin by fibroblast senescence via the p38 MAPK pathway. Aging (Albany NY). 2021;13:6406-6419 pubmed publisher
	Chai Z, Wang L, Zheng Y, Liang N, Wang X, Zheng Y, et al. PADI3 plays an antitumor role via the Hsp90/CKS1 pathway in colon cancer. Cancer Cell Int. 2019;19:277 pubmed publisher
	Miedlich S, Taya M, Young M, Hammes S. Paxillin and embryonic PolyAdenylation Binding Protein (ePABP) engage to regulate androgen-dependent Xenopus laevis oocyte maturation - A model of kinase-dependent regulation of protein expression. Mol Cell Endocrinol. 2017;448:87-97 pubmed publisher
	Hauser P, Dozmorov M, Bane B, Slobodov G, Culkin D, Hurst R. Abnormal expression of differentiation related proteins and proteoglycan core proteins in the urothelium of patients with interstitial cystitis. J Urol. 2008;179:764-9 pubmed
	Hansel D, Dhara S, Huang R, Ashfaq R, Deasel M, Shimada Y, et al. CDC2/CDK1 expression in esophageal adenocarcinoma and precursor lesions serves as a diagnostic and cancer progression marker and potential novel drug target. Am J Surg Pathol. 2005;29:390-9 pubmed
	Manes T, Zheng D, Tognin S, Woodard A, Marchisio P, Languino L. Alpha(v)beta3 integrin expression up-regulates cdc2, which modulates cell migration. J Cell Biol. 2003;161:817-26 pubmed

product information

Product Type :

Antibody

Product Name :

CDK1 Monoclonal Antibody (A17)

Catalog # :

33-1800

Quantity :

200 µg

Price :

US 456.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Amphibian, Chicken, Human, Mouse, Rat, Xenopus laevis

Applications :

ELISA: 0.1-1.0 µg/mL, Immunohistochemistry (Frozen): 5-10 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:50, Immunoprecipitation: 5 µg, Western Blot: 0.5-2 µg/mL

Species :

Amphibian, Chicken, Human, Mouse, Rat, Xenopus laevis

Clone :

A17

Isotype :

IgG2a, kappa

Storage :

-20°C

Description :

CDK1 (CDC2, P34CDC2) is a member of the Ser/Thr protein kinase family. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDK1 also play important regulatory roles in cell cycle control. Destruction of cyclin B during metaphase results in inactivation of CDK1, allowing mitotic exit and cell division. CDK1 also contributes to the control of DNA replication and can be inhibited by several transcriptional targets of p53, such as p21WAF. The ability of CDK1 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2. Research studies have shown that expression of CDK1 in the brain has been linked to both neurogenesis and apoptosis. In vertebrates, multiple cyclins like CDK1 have been identified, including a total of eight such regulatory proteins in mammals. Alternatively spliced transcript variants encoding different isoforms of CDK1 have been found.

Immunogen :

Recombinant protein consisiting of the carboxy-terminal two-thirds of the Xenopus p34cdc2

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.1-1.0 µg/mL, Immunohistochemistry (Frozen): 5-10 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:50, Immunoprecipitation: 5 µg, Western Blot: 0.5-2 µg/mL

Aliases :

5363; ABL1; cdc; CDC2; cdc-2; cdc2 kinase; CDC28A; Cdc2a; cdc2-a; cdc2Dm; cdc2x1.1; CDCDm; CDK1; Cdk-1; cdk1.S; CDK1/CDC2; CDK1-A; cdk1-b; Cdk1-PA; CDKN1; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; cell division control; cell division control protein 2 homolog; Cell division control protein 2 homolog 1; cell division control protein 2-A; cell division cycle 2 homolog A; cell division cycle 2 like; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2a; cell division protein kinase 1; Cell division protein kinase 1-A; CG5363; CG5363-PA; cyclin dependent kinase; cyclin dependent kinase 1; cyclin-dependent kinase; Cyclin-dependent kinase 1; cyclin-dependent kinase 1 S homeolog; cyclin-dependent kinase 1-A; D2366; Dcdc2; dCdk1; DKFZp686L20222; Dm cdc2; Dmcdc2; DmCdk1; Dmel CG5363; Dmel_CG5363; DNA polymerase delta catalytic subunit; DNA polymerase III; DNA-directed DNA polymerase delta POL3; group 4; HPR6; KCIP-1; l(2)31Eh; LD38718p; maturation-promoting factor; MGC111195; MGC53829; MPF; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase; p34 protein kinase 1; p34 ; p34cdc2; p34-cdc2 homologue; POL3; Protein 1054; protein 2; PSTAIR; TEX1; unnamed protein product; xcdc2; XELAEV_18036802mg; YDL102W

more info or order :

Invitrogen product webpage