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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
PLK1 Antibody Cocktail
catalog :
33-1700
quantity :
100 ug
price :
US 471.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
PL6/PL2
reactivity :
human, mouse, rat, Xenopus laevis
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, other
more info or order :
Invitrogen product webpage
citations: 44
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:500; loading ...; fig 3d
Paulmann C, Spallek R, Karpiuk O, Heider M, Sch xe4 ffer I, Zecha J, et al. The OTUD6B-LIN28B-MYC axis determines the proliferative state in multiple myeloma. EMBO J. 2022;41:e110871 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4d
Tan S, Chadha S, Liu Y, Gabasova E, Perera D, Ahmed K, et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell. 2017;169:1105-1118.e15 pubmed publisher
  • western blot; human; 1:500; fig s1
Engel K, Rudelius M, Slawska J, Jacobs L, Ahangarian Abhari B, Altmann B, et al. USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma. EMBO Mol Med. 2016;8:851-62 pubmed publisher
  • immunoprecipitation; human; fig 4
  • western blot; human; fig 4
Moudry P, Watanabe K, Wolanin K, Bartkova J, Wassing I, Watanabe S, et al. TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity. J Cell Biol. 2016;212:281-8 pubmed publisher
  • other; human; loading ...; fig st1
Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  • western blot; human; 1:500; fig 2
Kim J, Lee K, Rhee K. PLK1 regulation of PCNT cleavage ensures fidelity of centriole separation during mitotic exit. Nat Commun. 2015;6:10076 pubmed publisher
  • western blot; mouse; fig 1
Valianou M, Cox A, Pichette B, Hartley S, Paladhi U, Astrinidis A. Pharmacological inhibition of Polo-like kinase 1 (PLK1) by BI-2536 decreases the viability and survival of hamartin and tuberin deficient cells via induction of apoptosis and attenuation of autophagy. Cell Cycle. 2015;14:399-407 pubmed publisher
  • western blot; human
van der Lelij P, Stocsits R, Ladurner R, Petzold G, Kreidl E, Koch B, et al. SNW1 enables sister chromatid cohesion by mediating the splicing of sororin and APC2 pre-mRNAs. EMBO J. 2014;33:2643-58 pubmed publisher
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 2
Ando K, Ozaki T, Hirota T, Nakagawara A. NFBD1/MDC1 is phosphorylated by PLK1 and controls G2/M transition through the regulation of a TOPOII?-mediated decatenation checkpoint. PLoS ONE. 2013;8:e82744 pubmed publisher
  • western blot; human; 1:500; fig 5
Yu Y, Munger K. Human papillomavirus type 16 E7 oncoprotein inhibits the anaphase promoting complex/cyclosome activity by dysregulating EMI1 expression in mitosis. Virology. 2013;446:251-9 pubmed publisher
  • western blot; human; fig 7
Ovejero S, Ayala P, Bueno A, Sacristán M. Human Cdc14A regulates Wee1 stability by counteracting CDK-mediated phosphorylation. Mol Biol Cell. 2012;23:4515-25 pubmed publisher
  • western blot; human; 1:300
Kubara P, Kernéis Golsteyn S, Studény A, Lanser B, Meijer L, Golsteyn R. Human cells enter mitosis with damaged DNA after treatment with pharmacological concentrations of genotoxic agents. Biochem J. 2012;446:373-81 pubmed publisher
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 2
Hwang H, Ji J, Jang Y. Phosphorylation of Ran-binding protein-1 by Polo-like kinase-1 is required for interaction with Ran and early mitotic progression. J Biol Chem. 2011;286:33012-20 pubmed publisher
  • western blot; human; fig 1
Lee J, Kim J, Margolis R, Fotedar R. Substrate degradation by the anaphase promoting complex occurs during mitotic slippage. Cell Cycle. 2010;9:1792-801 pubmed
  • western blot; human; fig 1
Lee J, Kim J, Barbier V, Fotedar A, Fotedar R. DNA damage triggers p21WAF1-dependent Emi1 down-regulation that maintains G2 arrest. Mol Biol Cell. 2009;20:1891-902 pubmed publisher
  • immunoprecipitation; human; fig 2b
Wu Z, Yang X, Weber G, Liu X. Plk1 phosphorylation of TRF1 is essential for its binding to telomeres. J Biol Chem. 2008;283:25503-13 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Soond S, Barry S, Melino G, Knight R, Latchman D, Stephanou A. p73-mediated transcriptional activity is negatively regulated by polo-like kinase 1. Cell Cycle. 2008;7:1214-23 pubmed
  • western blot; human; fig 2
Flatmark K, Nome R, Folkvord S, Bratland A, Rasmussen H, Ellefsen M, et al. Radiosensitization of colorectal carcinoma cell lines by histone deacetylase inhibition. Radiat Oncol. 2006;1:25 pubmed
  • western blot; human; fig 1B
Lyng H, Landsverk K, Kristiansen E, DeAngelis P, Ree A, Myklebost O, et al. Response of malignant B lymphocytes to ionizing radiation: gene expression and genotype. Int J Cancer. 2005;115:935-42 pubmed
  • immunocytochemistry; human; 5 ug/ml; fig 6
  • western blot; human; fig 2B
Hansen D, Loktev A, Ban K, Jackson P. Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1. Mol Biol Cell. 2004;15:5623-34 pubmed
  • western blot; human; fig 6
Crawford D, Piwnica Worms H. The G(2) DNA damage checkpoint delays expression of genes encoding mitotic regulators. J Biol Chem. 2001;276:37166-77 pubmed
Zecha J, Gabriel W, Spallek R, Chang Y, Mergner J, Wilhelm M, et al. Linking post-translational modifications and protein turnover by site-resolved protein turnover profiling. Nat Commun. 2022;13:165 pubmed publisher
Guarino Almeida E, Renaudin X, Venkitaraman A. A kinase-independent function for AURORA-A in replisome assembly during DNA replication initiation. Nucleic Acids Res. 2020;: pubmed publisher
Jemaà M, Kifagi C, Serrano S, Massoumi R. Preferential Killing of Tetraploid Colon Cancer Cells by Targeting the Mitotic Kinase PLK1. Cell Physiol Biochem. 2020;54:303-320 pubmed publisher
Dietachmayr M, Rathakrishnan A, Karpiuk O, von Zweydorf F, Engleitner T, Fernández Sáiz V, et al. Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival. Nat Commun. 2020;11:1268 pubmed publisher
Huggins D, Hardwick B, Sharma P, Emery A, Laraia L, Zhang F, et al. Development of a Novel Cell-Permeable Protein-Protein Interaction Inhibitor for the Polo-box Domain of Polo-like Kinase 1. ACS Omega. 2020;5:822-831 pubmed publisher
Sharma P, Mahen R, Rossmann M, Stokes J, Hardwick B, Huggins D, et al. A cryptic hydrophobic pocket in the polo-box domain of the polo-like kinase PLK1 regulates substrate recognition and mitotic chromosome segregation. Sci Rep. 2019;9:15930 pubmed publisher
Yabuno Y, Uchihashi T, Sasakura T, Shimizu H, Naito Y, Fukushima K, et al. Clathrin heavy chain phosphorylated at T606 plays a role in proper cell division. Cell Cycle. 2019;18:1976-1994 pubmed publisher
Fang C, Kuo H, Hsu S, Yih L. HSP70 is required for the proper assembly of pericentriolar material and function of mitotic centrosomes. Cell Div. 2019;14:4 pubmed publisher
Revinski D, Zaragosi L, Boutin C, Ruiz García S, Deprez M, Thomé V, et al. CDC20B is required for deuterosome-mediated centriole production in multiciliated cells. Nat Commun. 2018;9:4668 pubmed publisher
Ovejero S, Ayala P, Malumbres M, Pimentel Muiños F, Bueno A, Sacristán M. Biochemical analyses reveal amino acid residues critical for cell cycle-dependent phosphorylation of human Cdc14A phosphatase by cyclin-dependent kinase 1. Sci Rep. 2018;8:11871 pubmed publisher
de Carcer G, Venkateswaran S, Salgueiro L, El Bakkali A, Somogyi K, Rowald K, et al. Plk1 overexpression induces chromosomal instability and suppresses tumor development. Nat Commun. 2018;9:3012 pubmed publisher
Adam K, Cartel M, Lambert M, David L, Yuan L, Besson A, et al. A PIM-CHK1 signaling pathway regulates PLK1 phosphorylation and function during mitosis. J Cell Sci. 2018;131: pubmed publisher
Fung E, Richter C, Yang H, Schäffer I, Fischer R, Kessler B, et al. FBXL13 directs the proteolysis of CEP192 to regulate centrosome homeostasis and cell migration. EMBO Rep. 2018;19: pubmed publisher
Narváez A, Ber S, Crooks A, Emery A, Hardwick B, Guarino Almeida E, et al. Modulating Protein-Protein Interactions of the Mitotic Polo-like Kinases to Target Mutant KRAS. Cell Chem Biol. 2017;24:1017-1028.e7 pubmed publisher
de Carcer G, Wachowicz P, Martínez Martínez S, Oller J, Méndez Barbero N, Escobar B, et al. Plk1 regulates contraction of postmitotic smooth muscle cells and is required for vascular homeostasis. Nat Med. 2017;23:964-974 pubmed publisher
Wachowicz P, Fernández Miranda G, Marugán C, Escobar B, de Cárcer G. Genetic depletion of Polo-like kinase 1 leads to embryonic lethality due to mitotic aberrancies. Bioessays. 2016;38 Suppl 1:S96-S106 pubmed publisher
Hasvold G, Lund Andersen C, Lando M, Patzke S, Hauge S, Suo Z, et al. Hypoxia-induced alterations of G2 checkpoint regulators. Mol Oncol. 2016;10:764-73 pubmed publisher
Kashima L, Idogawa M, Mita H, Shitashige M, Yamada T, Ogi K, et al. CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation. J Biol Chem. 2012;287:12975-84 pubmed publisher
Kim J, Lee J, Margolis R, Fotedar R. SP600125 suppresses Cdk1 and induces endoreplication directly from G2 phase, independent of JNK inhibition. Oncogene. 2010;29:1702-16 pubmed publisher
Mailand N, Bekker Jensen S, Bartek J, Lukas J. Destruction of Claspin by SCFbetaTrCP restrains Chk1 activation and facilitates recovery from genotoxic stress. Mol Cell. 2006;23:307-18 pubmed
Hansen D, Tung J, Jackson P. CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit. Proc Natl Acad Sci U S A. 2006;103:608-13 pubmed
Kho P, Wang Z, Zhuang L, Li Y, Chew J, Ng H, et al. p53-regulated transcriptional program associated with genotoxic stress-induced apoptosis. J Biol Chem. 2004;279:21183-92 pubmed
Kraft C, Herzog F, Gieffers C, Mechtler K, Hagting A, Pines J, et al. Mitotic regulation of the human anaphase-promoting complex by phosphorylation. EMBO J. 2003;22:6598-609 pubmed
product information
Product Type :
Antibody
Product Name :
PLK1 Antibody Cocktail
Catalog # :
33-1700
Quantity :
100 ug
Price :
US 471.00
Clonality :
Cocktail
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Mouse, Non-human primate, Rat, Xenopus laevis
Applications :
ELISA: 0.1-1.0 ug/mL, Immunocytochemistry: 1-5 ug/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: 5 ug, Western Blot: 1-2 ug/mL
Species :
Human, Mouse, Non-human primate, Rat, Xenopus laevis
Clone :
PL6/PL2
Isotype :
IgG1, kappa/IgG2b, kappa
Storage :
-20 C
Description :
PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.
Immunogen :
A His-tagged Plk fusion protein (C-term Plk1) containing amino acids 402-603 of the human Plk1 protein(
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-1.0 ug/mL, Immunocytochemistry: 1-5 ug/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: 5 ug, Western Blot: 1-2 ug/mL
Aliases :
cell cycle regulated protein kinase; Plk; PLK 1; Plk1; PLK-1; plk1.S; plx1; polo (Drosophia)-like kinase; polo like kinase; polo like kinase 1; polo like kinase 1 S homeolog; polo-like kinase; polo-like kinase 1; polo-like kinase homolog; polo-like protein kinase; serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14; Unknown (protein for MGC:133927); XELAEV_18047692mg
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA