Occludin Monoclonal Antibody (OC-3F10), HRP | Invitrogen 33-1520 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Occludin Monoclonal Antibody (OC-3F10), HRP

catalog :

33-1520

quantity :

100 ug

price :

US 520.00

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

OC-3F10

reactivity :

human, mouse, rat, dogs

application :

western blot, ELISA

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 2b	Ni Y, Teng T, Li R, Simonyi A, Sun G, Lee J. TNFα alters occludin and cerebral endothelial permeability: Role of p38MAPK. PLoS ONE. 2017;12:e0170346 pubmed publisher
western blot; human	Mishra R, Singh S. HIV-1 Tat C modulates expression of miRNA-101 to suppress VE-cadherin in human brain microvascular endothelial cells. J Neurosci. 2013;33:5992-6000 pubmed publisher
	Kakiuchi A, Kakuki T, Ohwada K, Kurose M, Kondoh A, Obata K, et al. HDAC inhibitors suppress the proliferation, migration and invasiveness of human head and neck squamous cell carcinoma cells via p63‑mediated tight junction molecules and p21‑mediated growth arrest. Oncol Rep. 2021;45: pubmed publisher
	Deng Z, Zhou L, Wang Y, Liao S, Huang Y, Shan Y, et al. Astrocyte-derived VEGF increases cerebral microvascular permeability under high salt conditions. Aging (Albany NY). 2020;12:11781-11793 pubmed publisher
	Yamazaki Y, Shinohara M, Yamazaki A, Ren Y, Asmann Y, Kanekiyo T, et al. ApoE (Apolipoprotein E) in Brain Pericytes Regulates Endothelial Function in an Isoform-Dependent Manner by Modulating Basement Membrane Components. Arterioscler Thromb Vasc Biol. 2020;40:128-144 pubmed publisher
	Annunziata P, Cioni C, Masi G, Tassi M, Marotta G, Severi S. Fingolimod reduces circulating tight-junction protein levels and in vitro peripheral blood mononuclear cells migration in multiple sclerosis patients. Sci Rep. 2018;8:15371 pubmed publisher
	Noda S, Tanabe S, Suzuki T. Differential effects of flavonoids on barrier integrity in human intestinal Caco-2 cells. J Agric Food Chem. 2012;60:4628-33 pubmed publisher
	Jain S, Suzuki T, Seth A, Samak G, Rao R. Protein kinase C? phosphorylates occludin and promotes assembly of epithelial tight junctions. Biochem J. 2011;437:289-99 pubmed publisher
	Marino M, Zheng G, Chiovato L, Pinchera A, Brown D, Andrews D, et al. Role of megalin (gp330) in transcytosis of thyroglobulin by thyroid cells. A novel function in the control of thyroid hormone release. J Biol Chem. 2000;275:7125-37 pubmed

product information

Product Type :

Antibody

Product Name :

Occludin Monoclonal Antibody (OC-3F10), HRP

Catalog # :

33-1520

Quantity :

100 ug

Price :

US 520.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Rat

Applications :

ELISA: 0.1-1.0 ug/mL, Western Blot: 2 ug/mL

Species :

Canine, Human, Mouse, Rat

Clone :

OC-3F10

Isotype :

IgG1, kappa

Storage :

4 C

Description :

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

Immunogen :

GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.1-1.0 ug/mL, Western Blot: 2 ug/mL

Aliases :

AI503564; BLCPMG; Occludin; occludin 1B; Ocl; Ocln; phosphatase 1, regulatory subunit 115; PPP1R115; tight junction protein occludin; tight junction structural protein

more info or order :

Invitrogen product webpage