RANGAP1 Monoclonal Antibody (19C7) | Invitrogen 33-0800 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

RANGAP1 Monoclonal Antibody (19C7)

catalog :

33-0800

quantity :

100 µg

price :

US 449.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

19C7

reactivity :

African green monkey, human, mouse, rat

application :

western blot, ELISA, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 6	Sloan E, Tatham M, Groslambert M, Glass M, Orr A, Hay R, et al. Analysis of the SUMO2 Proteome during HSV-1 Infection. PLoS Pathog. 2015;11:e1005059 pubmed publisher
immunocytochemistry; human; fig 4 western blot; human; fig 5	Chow K, Elgort S, Dasso M, Powers M, Ullman K. The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function. Mol Biol Cell. 2014;25:160-8 pubmed publisher
immunocytochemistry; human western blot; human	Hashizume C, Kobayashi A, Wong R. Down-modulation of nucleoporin RanBP2/Nup358 impaired chromosomal alignment and induced mitotic catastrophe. Cell Death Dis. 2013;4:e854 pubmed publisher
immunocytochemistry; human; 1:1000; fig 8	Funakoshi T, Clever M, Watanabe A, Imamoto N. Localization of Pom121 to the inner nuclear membrane is required for an early step of interphase nuclear pore complex assembly. Mol Biol Cell. 2011;22:1058-69 pubmed publisher
western blot; human; fig 3	Tatham M, Rodriguez M, Xirodimas D, Hay R. Detection of protein SUMOylation in vivo. Nat Protoc. 2009;4:1363-71 pubmed publisher
western blot; human	Schimmel J, Larsen K, Matic I, van Hagen M, Cox J, Mann M, et al. The ubiquitin-proteasome system is a key component of the SUMO-2/3 cycle. Mol Cell Proteomics. 2008;7:2107-22 pubmed publisher
western blot; human; fig 1	Vertegaal A, Andersen J, Ogg S, Hay R, Mann M, Lamond A. Distinct and overlapping sets of SUMO-1 and SUMO-2 target proteins revealed by quantitative proteomics. Mol Cell Proteomics. 2006;5:2298-310 pubmed
western blot; human	Rosas Acosta G, Russell W, Deyrieux A, Russell D, Wilson V. A universal strategy for proteomic studies of SUMO and other ubiquitin-like modifiers. Mol Cell Proteomics. 2005;4:56-72 pubmed
immunocytochemistry; African green monkey; fig 5	Saitoh H, Hinchey J. Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3. J Biol Chem. 2000;275:6252-8 pubmed
	Sloan E, Orr A, Everett R. MORC3, a Component of PML Nuclear Bodies, Has a Role in Restricting Herpes Simplex Virus 1 and Human Cytomegalovirus. J Virol. 2016;90:8621-33 pubmed publisher
	Datta S, Snow C, Paschal B. A pathway linking oxidative stress and the Ran GTPase system in progeria. Mol Biol Cell. 2014;25:1202-15 pubmed publisher
	Ogawa Y, Miyamoto Y, Oka M, Yoneda Y. The interaction between importin-? and Nup153 promotes importin-?/?-mediated nuclear import. Traffic. 2012;13:934-46 pubmed publisher
	Rosendorff A, Sakakibara S, Lu S, Kieff E, Xuan Y, DiBacco A, et al. NXP-2 association with SUMO-2 depends on lysines required for transcriptional repression. Proc Natl Acad Sci U S A. 2006;103:5308-13 pubmed
	Yamaguchi T, Sharma P, Athanasiou M, Kumar A, Yamada S, Kuehn M. Mutation of SENP1/SuPr-2 reveals an essential role for desumoylation in mouse development. Mol Cell Biol. 2005;25:5171-82 pubmed
	Hang J, Dasso M. Association of the human SUMO-1 protease SENP2 with the nuclear pore. J Biol Chem. 2002;277:19961-6 pubmed
	Joseph J, Tan S, Karpova T, McNally J, Dasso M. SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles. J Cell Biol. 2002;156:595-602 pubmed
	Saitoh H, Pizzi M, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002;277:4755-63 pubmed

product information

Product Type :

Antibody

Product Name :

RANGAP1 Monoclonal Antibody (19C7)

Catalog # :

33-0800

Quantity :

100 µg

Price :

US 449.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 1-2 µg/mL, Western Blot: 0.1-1.0 µg/mL

Species :

Human, Mouse, Rat

Clone :

19C7

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.

Immunogen :

A fusion protein consisting of the first 203 amino-terminal residues of the mouse RanGAP-1 protein.

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 1-2 µg/mL, Western Blot: 0.1-1.0 µg/mL

Aliases :

C79654; fug1; KIAA1835; MGC20266; mKIAA1835; RAN GTPase activating protein 1; RAN GTPase activating protein 1 a; Ran GTPase activating protein 1 b; Ran GTPase activating protein 1 L homeolog; Ran GTPase activating protein 1 S homeolog; ran GTPase-activating protein 1; RANGAP; RanGAP1; rangap1.L; rangap1.S; rangap1a; rangap1-a; rangap1b; rangap1-b; rna1p; SD; segregation distorter homolog; segregation distortion; XELAEV_18023679mg; XELAEV_18025680mg

more info or order :

Invitrogen product webpage