HSF1 Monoclonal Antibody (4B4)
Invitrogen
catalog: MA5-14622

rat monoclonal (4B4)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
citations: 11

quantity: 500 uL
price: US 474.00
to the supplier

HSF1 Polyclonal Antibody
Invitrogen
catalog: PA3-017

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
citations: 2

Western blot analysis of Heat Shock Factor 1 (HSF1) was performed by loading 50µg of the indicated whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSF1 rabbit polyclonal antibody (Product # PA3-017) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least one hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

Immunoprecipitation of Heat Shock Factor 1 (HSF 1) was performed on HeLa cells. Antigen:antibody complexes were formed by incubating 500µg whole cell lysate with 2µg of HSF1 polyclonal antibody (Product # PA3-017) overnight on a rocking platform at 4°C. Immune complexes were captured on 50µl Protein A/G Plus Agarose (Product #20423), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSF1 polyclonal antibody (Product # PA3-017) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean Blot IP Detection Reagent-HRP (Product # 21232) at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

Immunofluorescent analysis of Heat Shock Factor 1 (HSF1) (green) in HeLa and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a HSF1 polyclonal antibody (Product # PA3-017), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
quantity: 100 uL
price: US 451.00
to the supplier

HSF1 Monoclonal Antibody (10H8)
Invitrogen
catalog: MA5-27688

rat monoclonal (10H8)
reactivity: Chinese hamsters, guinea pig, human, mouse, rat, bovine, domestic rabbit, rhesus macaque
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 1

quantity: 100 ug
price: US 504.00
to the supplier

Phospho-HSF1 (Ser326) Recombinant Rabbit Monoclonal Antibody (SU31-03)
Invitrogen
catalog: MA5-32105

domestic rabbit recombinant (SU31-03)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1

quantity: 100 uL
price: US 464.00
to the supplier

HSF1 Polyclonal Antibody
Invitrogen
catalog: PA5-89420

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

quantity: 100 uL
price: US 454.00
to the supplier

HSF1 Polyclonal Antibody
Invitrogen
catalog: PA5-12181

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry

quantity: 400 uL
price: US 474.00
to the supplier

Phospho-HSF1 (Ser303) Polyclonal Antibody
Invitrogen
catalog: PA5-36851

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 470.00
to the supplier

Phospho-HSF1 (Ser303) Polyclonal Antibody
Invitrogen
catalog: PA5-37598

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

Phospho-HSF1 (Thr142) Polyclonal Antibody
Invitrogen
catalog: PA5-37600

domestic rabbit polyclonal
reactivity: human, mouse
application: immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

Phospho-HSF1 (Ser121) Polyclonal Antibody
Invitrogen
catalog: PA5-39718

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 ug
price: US 464.00
to the supplier

HSF1 Recombinant Rabbit Monoclonal Antibody (009)
Invitrogen
catalog: MA5-29330

domestic rabbit recombinant (9)
reactivity: human
application: ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 414.00
to the supplier

Phospho-HSF1 (Ser326) Recombinant Rabbit Monoclonal Antibody (2A7)
Invitrogen
catalog: MA5-33167

domestic rabbit recombinant (2A7)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 491.00
to the supplier

Phospho-HSF1 (Ser326) Recombinant Rabbit Monoclonal Antibody (ARC1601)
Invitrogen
catalog: MA5-35889

domestic rabbit recombinant (ARC1601)
reactivity: human
application: western blot, ELISA

quantity: 100 uL
price: US 491.00
to the supplier

Phospho-HSF1 (Ser326) Polyclonal Antibody
Invitrogen
catalog: PA5-101018

domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

Phospho-HSF1 (Ser307) Polyclonal Antibody
Invitrogen
catalog: PA5-105806

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 454.00
to the supplier

HSF1 Monoclonal Antibody (4D5F4)
Invitrogen
catalog: MA5-38540

mouse monoclonal (4D5F4)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 ug
price: US 484.00
to the supplier

HSF1 Monoclonal Antibody (10H8), APC
Invitrogen
catalog: MA5-45127

rat monoclonal (10H8)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, bovine, domestic rabbit
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 410.00
to the supplier

HSF1 Monoclonal Antibody (10H8), FITC
Invitrogen
catalog: MA5-45128

rat monoclonal (10H8)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, bovine, domestic rabbit
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 410.00
to the supplier

HSF1 Monoclonal Antibody (10H8), PerCP
Invitrogen
catalog: MA5-45129

rat monoclonal (10H8)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, bovine, domestic rabbit
conjugate: PerCP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 410.00
to the supplier

HSF1 Monoclonal Antibody (10H8), PE
Invitrogen
catalog: MA5-45130

rat monoclonal (10H8)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, bovine, domestic rabbit
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 420.00
to the supplier

HSF1 Monoclonal Antibody (10H4), APC
Invitrogen
catalog: MA5-45709

rat monoclonal (10H4)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, bovine, domestic rabbit
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 444.00
to the supplier

HSF1 Monoclonal Antibody (10H4), FITC
Invitrogen
catalog: MA5-45710

rat monoclonal (10H4)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, bovine, domestic rabbit
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 444.00
to the supplier

HSF1 Monoclonal Antibody (10H4), PerCP
Invitrogen
catalog: MA5-45711

rat monoclonal (10H4)
reactivity: Chinese hamsters, guinea pig, human, mouse, rat, bovine, domestic rabbit
conjugate: PerCP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 444.00
to the supplier

HSF1 Monoclonal Antibody (4B4), APC
Invitrogen
catalog: MA5-45712

rat monoclonal (4B4)
reactivity: Chinese hamsters, guinea pig, human, mouse, rat, bovine, domestic rabbit
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 444.00
to the supplier

HSF1 Monoclonal Antibody (4B4), FITC
Invitrogen
catalog: MA5-45713

rat monoclonal (4B4)
reactivity: Chinese hamsters, guinea pig, human, mouse, rat, bovine, domestic rabbit
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 444.00
to the supplier

HSF1 Monoclonal Antibody (4B4), PerCP
Invitrogen
catalog: MA5-45714

rat monoclonal (4B4)
reactivity: Chinese hamsters, guinea pig, human, mouse, rat, bovine, domestic rabbit
conjugate: PerCP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 444.00
to the supplier

HSF1 Recombinant Rabbit Monoclonal Antibody (5C7)
Invitrogen
catalog: MA5-49957

domestic rabbit recombinant (5C7)
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 414.00
to the supplier

Phospho-HSF1 (Ser303) Polyclonal Antibody
Invitrogen
catalog: PA5-114582

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

HSF1 Recombinant Rabbit Monoclonal Antibody (23GB2715)
Invitrogen
catalog: MA5-53482

domestic rabbit recombinant (23GB2715)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

quantity: 100 uL
price: US 539.00
to the supplier

HSF1 Recombinant Rabbit Monoclonal Antibody (23GB2715)
Invitrogen
catalog: MA5-53483

domestic rabbit recombinant (23GB2715)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

quantity: 20 uL
price: US 199.00
to the supplier

Phospho-HSF1 (Ser303, Ser307) Recombinant Rabbit Monoclonal Antibody (24GB5445)
Invitrogen
catalog: MA5-57576

domestic rabbit recombinant (24GB5445)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

quantity: 100 uL
price: US 539.00
to the supplier

Phospho-HSF1 (Ser303, Ser307) Recombinant Rabbit Monoclonal Antibody (24GB5445)
Invitrogen
catalog: MA5-57577

domestic rabbit recombinant (24GB5445)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

quantity: 20 uL
price: US 189.00
to the supplier

Phospho-HSF1 (Ser326) Recombinant Rabbit Monoclonal Antibody (24GB5400)
Invitrogen
catalog: MA5-57578

domestic rabbit recombinant (24GB5400)
reactivity: human, mouse
application: western blot, immunocytochemistry, flow cytometry

quantity: 100 uL
price: US 539.00
to the supplier

Phospho-HSF1 (Ser326) Recombinant Rabbit Monoclonal Antibody (24GB5400)
Invitrogen
catalog: MA5-57579

domestic rabbit recombinant (24GB5400)
reactivity: human, mouse
application: western blot, immunocytochemistry, flow cytometry

quantity: 20 uL
price: US 189.00
to the supplier