Invitrogen H-Ras antibody

Pan Ras Monoclonal Antibody (Ras10)
Invitrogen
catalog: MA1-012X

mouse monoclonal (RAS10)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 17

Immunofluorescent analysis of Ras in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Ras monoclonal antibody (Product # MA1-012) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Ras staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Ras monoclonal antibody (Product # MA1-012) at a dilution of 1:200 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Western blot analysis of Ras was performed by loading 50ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing Ras (Product # MA1-012) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:20000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico(Product #34077).
quantity: 20 ug
price: US 177.00
to the supplier

Pan Ras Monoclonal Antibody (Ras10)
Invitrogen
catalog: MA1-012

quantity: 100 ug
price: US 502.00
to the supplier

H-Ras Monoclonal Antibody (Y13-259)
Invitrogen
catalog: 33-7200

rat monoclonal (Y13-259)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation, other
citations: 4

quantity: 50 ug
price: US 520.00
to the supplier

H-Ras Monoclonal Antibody (H-Ras-03)
Invitrogen
catalog: MA1-19726

mouse monoclonal (H-Ras-03)
reactivity: human
application: western blot

quantity: 100 ug
price: US 464.00
to the supplier

H-Ras Polyclonal Antibody
Invitrogen
catalog: PA5-14863

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 400 uL
price: US 474.00
to the supplier

Pan Ras Polyclonal Antibody
Invitrogen
catalog: PA5-78035

domestic rabbit polyclonal
reactivity: thirteen-lined ground squirrel, human, rat, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 514.00
to the supplier

H-Ras Polyclonal Antibody
Invitrogen
catalog: PA5-79395

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

quantity: 100 ug
price: US 410.00
to the supplier

Pan Ras Recombinant Rabbit Monoclonal Antibody (SA39-05)
Invitrogen
catalog: MA5-31963

domestic rabbit recombinant (SA39-05)
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry

quantity: 100 uL
price: US 474.00
to the supplier

Pan Ras Recombinant Rabbit Monoclonal Antibody (JF10-11)
Invitrogen
catalog: MA5-32561

domestic rabbit recombinant (JF10-11)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunocytochemistry

quantity: 100 uL
price: US 464.00
to the supplier

H-Ras Polyclonal Antibody
Invitrogen
catalog: PA5-80694

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation

quantity: 100 uL
price: US 319.00
to the supplier

Pan Ras Polyclonal Antibody
Invitrogen
catalog: PA5-85947

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 454.00
to the supplier

H-Ras Polyclonal Antibody
Invitrogen
catalog: PA5-87037

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 481.00
to the supplier

H-Ras Polyclonal Antibody
Invitrogen
catalog: PA5-94930

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 ug
price: US 464.00
to the supplier

H-Ras Polyclonal Antibody
Invitrogen
catalog: PA5-88424

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry

quantity: 100 uL
price: US 464.00
to the supplier

H-Ras Polyclonal Antibody
Invitrogen
catalog: PA5-96852

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

H-Ras Recombinant Rabbit Monoclonal Antibody (4Q7M5)
Invitrogen
catalog: MA5-35323

domestic rabbit recombinant (4Q7M5)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

Pan Ras Recombinant Rabbit Monoclonal Antibody (ARC1112)
Invitrogen
catalog: MA5-35567

domestic rabbit recombinant (ARC1112)
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry

quantity: 100 uL
price: US 474.00
to the supplier

Pan Ras Polyclonal Antibody
Invitrogen
catalog: PA5-104464

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

Pan Ras Recombinant Rabbit Monoclonal Antibody (ARC2369)
Invitrogen
catalog: MA5-37994

domestic rabbit recombinant (ARC2369)
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry

quantity: 100 uL
price: US 484.00
to the supplier

H-Ras Recombinant Rabbit Monoclonal Antibody (23GB6035)
Invitrogen
catalog: MA5-53940

domestic rabbit recombinant (23GB6035)
reactivity: human, mouse, rat
application: western blot, flow cytometry

quantity: 100 uL
price: US 539.00
to the supplier

H-Ras Recombinant Rabbit Monoclonal Antibody (23GB6035)
Invitrogen
catalog: MA5-53941

domestic rabbit recombinant (23GB6035)
reactivity: human, mouse, rat
application: western blot, flow cytometry

quantity: 20 uL
price: US 199.00
to the supplier

H-Ras Monoclonal Antibody (24GB5695)
Invitrogen
catalog: MA5-57424

mouse monoclonal (24GB5695)
reactivity: human, mouse, rat
application: western blot

quantity: 100 uL
price: US 539.00
to the supplier

H-Ras Monoclonal Antibody (24GB5695)
Invitrogen
catalog: MA5-57425

mouse monoclonal (24GB5695)
reactivity: human, mouse, rat
application: western blot

quantity: 20 uL
price: US 189.00
to the supplier

H-Ras Monoclonal Antibody (24GB5700)
Invitrogen
catalog: MA5-57426

mouse monoclonal (24GB5700)
reactivity: human, mouse, rat
application: western blot

quantity: 100 uL
price: US 539.00
to the supplier

H-Ras Monoclonal Antibody (24GB5700)
Invitrogen
catalog: MA5-57427

mouse monoclonal (24GB5700)
reactivity: human, mouse, rat
application: western blot

quantity: 20 uL
price: US 189.00
to the supplier