alpha Tubulin Monoclonal Antibody (B-5-1-2), Alexa Fluor 488

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

alpha Tubulin Monoclonal Antibody (B-5-1-2), Alexa Fluor 488

catalog :

322588

quantity :

100 ug

price :

US 562.00

clonality :

monoclonal

host :

mouse

conjugate :

AF488

clone name :

B-5-1-2

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 39

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:100; loading ...; fig 6a	Balboula A, Nguyen A, Gentilello A, Quartuccio S, Drutovic D, Solc P, et al. Haspin kinase regulates microtubule-organizing center clustering and stability through Aurora kinase C in mouse oocytes. J Cell Sci. 2016;129:3648-3660 pubmed
immunocytochemistry; human; 0.5 ug/ml; fig 2	Cunningham D, Parajuli K, Zhang C, Wang G, Mei J, Zhang Q, et al. Monomethyl Auristatin E Phosphate Inhibits Human Prostate Cancer Growth. Prostate. 2016;76:1420-30 pubmed publisher
immunocytochemistry; human; 1:1000; fig 4	Rao S, Flores Rodriguez N, Page S, Wong C, Robinson P, Chircop M. The Clathrin-dependent Spindle Proteome. Mol Cell Proteomics. 2016;15:2537-53 pubmed publisher
immunocytochemistry; human; fig 7 immunocytochemistry; mouse; fig 2	Stritt S, Nurden P, Favier R, Favier M, Ferioli S, Gotru S, et al. Defects in TRPM7 channel function deregulate thrombopoiesis through altered cellular Mg(2+) homeostasis and cytoskeletal architecture. Nat Commun. 2016;7:11097 pubmed publisher
immunocytochemistry; human; fig s3	Müller M, Mönkemöller V, Hennig S, Hubner W, Huser T. Open-source image reconstruction of super-resolution structured illumination microscopy data in ImageJ. Nat Commun. 2016;7:10980 pubmed publisher
immunocytochemistry; mouse; 1:100; fig 3	Balboula A, Stein P, Schultz R, Schindler K. RBBP4 regulates histone deacetylation and bipolar spindle assembly during oocyte maturation in the mouse. Biol Reprod. 2015;92:105 pubmed publisher
immunohistochemistry; mouse	Pratt S, Shah S, Ward C, Kerr J, Stains J, Lovering R. Recovery of altered neuromuscular junction morphology and muscle function in mdx mice after injury. Cell Mol Life Sci. 2015;72:153-64 pubmed publisher
immunocytochemistry; mouse; 1:100; fig 7a	Balboula A, Schindler K. Selective disruption of aurora C kinase reveals distinct functions from aurora B kinase during meiosis in mouse oocytes. PLoS Genet. 2014;10:e1004194 pubmed publisher
	Lee I, Adhikari D, Carroll J. Miro1 depletion disrupts spatial distribution of mitochondria and leads to oocyte maturation defects. Front Cell Dev Biol. 2022;10:986454 pubmed publisher
	Xue Y, Meng T, Ouyang Y, Liu S, Guo J, Wang Z, et al. Miro1 regulates mitochondrial homeostasis and meiotic resumption of mouse oocyte. J Cell Physiol. 2022;237:4477-4486 pubmed publisher
	Li J, Chang H, Yi Z, Zhang C, Sun Q, Qian W. Transient inhibition of CDK2 activity prevents oocyte meiosis I completion and egg activation in mouse. J Cell Physiol. 2022;237:4317-4325 pubmed publisher
	Yan F, Ouyang Y, Meng T, Zhang H, Yue W, Zhang X, et al. Toxic effects of AZD1208 on mouse oocytes and its possible mechanisms. J Cell Physiol. 2022;237:3661-3670 pubmed publisher
	Kratka C, Drutovic D, Blengini C, Schindler K. Using ZINC08918027 inhibitor to determine Aurora kinase-chromosomal passenger complex isoforms in mouse oocytes. BMC Res Notes. 2022;15:96 pubmed publisher
	Londo xf1 o V xe1 squez D, Rodriguez Lukey K, Behura S, Balboula A. Microtubule organizing centers regulate spindle positioning in mouse oocytes. Dev Cell. 2022;57:197-211.e3 pubmed publisher
	Blengini C, Nguyen A, Aboelenain M, Schindler K. Age-dependent integrity of the meiotic spindle assembly checkpoint in females requires Aurora kinase B. Aging Cell. 2021;20:e13489 pubmed publisher
	Zhang H, Li J, Ouyang Y, Meng T, Zhang C, Yue W, et al. Cell Division Cycle 5-Like Regulates Metaphase-to-Anaphase Transition in Meiotic Oocyte. Front Cell Dev Biol. 2021;9:671685 pubmed publisher
	Blengini C, Ibrahimian P, Vaskovicova M, Drutovic D, Solc P, Schindler K. Aurora kinase A is essential for meiosis in mouse oocytes. PLoS Genet. 2021;17:e1009327 pubmed publisher
	Coleman A, Joca H, Shi G, Lederer W, Ward C. Tubulin acetylation increases cytoskeletal stiffness to regulate mechanotransduction in striated muscle. J Gen Physiol. 2021;153: pubmed publisher
	Tyc K, El Yakoubi W, Bag A, Landis J, Zhan Y, Treff N, et al. Exome sequencing links CEP120 mutation to maternally derived aneuploid conception risk. Hum Reprod. 2020;35:2134-2148 pubmed publisher
	Devlin D, Nozawa K, Ikawa M, Matzuk M. Knockout of family with sequence similarity 170 member A (Fam170a) causes male subfertility, while Fam170b is dispensable in mice†. Biol Reprod. 2020;103:205-222 pubmed publisher
	Nourreddine S, Lavoie G, Paradis J, Ben El Kadhi K, Méant A, Aubert L, et al. NF45 and NF90 Regulate Mitotic Gene Expression by Competing with Staufen-Mediated mRNA Decay. Cell Rep. 2020;31:107660 pubmed publisher
	Devlin D, Agrawal Zaneveld S, Nozawa K, Han X, Moye A, Liang Q, et al. Knockout of mouse receptor accessory protein 6 leads to sperm function and morphology defects†. Biol Reprod. 2020;102:1234-1247 pubmed publisher
	Li J, Ouyang Y, Zhang C, Qian W, Sun Q. The cyclin B2/CDK1 complex inhibits separase activity in mouse oocyte meiosis I. Development. 2019;146: pubmed publisher
	Rodriguez A, Briley S, Patton B, Tripurani S, Rajapakshe K, Coarfa C, et al. Loss of the E2 SUMO-conjugating enzyme Ube2i in oocytes during ovarian folliculogenesis causes infertility in mice. Development. 2019;146: pubmed publisher
	White C, Jayasekara W, Picard D, Chen J, Watkins D, Cain J, et al. A Sexually Dimorphic Role for STAT3 in Sonic Hedgehog Medulloblastoma. Cancers (Basel). 2019;11: pubmed publisher
	Rayner R, Makena P, Prasad G, Cormet Boyaka E. Cigarette and ENDS preparations differentially regulate ion channels and mucociliary clearance in primary normal human bronchial 3D cultures. Am J Physiol Lung Cell Mol Physiol. 2019;317:L295-L302 pubmed publisher
	Kim Y, Choi J, Yang M, Hong S, Lee C, Kubota Y, et al. A MST1-FOXO1 cascade establishes endothelial tip cell polarity and facilitates sprouting angiogenesis. Nat Commun. 2019;10:838 pubmed publisher
	Nguyen T, Mitchison T, Wühr M. Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos. Methods Mol Biol. 2019;1920:17-32 pubmed publisher
	De Leo A, Deng Z, Vladimirova O, Chen H, Dheekollu J, Calderon A, et al. LANA oligomeric architecture is essential for KSHV nuclear body formation and viral genome maintenance during latency. PLoS Pathog. 2019;15:e1007489 pubmed publisher
	Rayner R, Makena P, Prasad G, Cormet Boyaka E. Optimization of Normal Human Bronchial Epithelial (NHBE) Cell 3D Cultures for in vitro Lung Model Studies. Sci Rep. 2019;9:500 pubmed publisher
	Liccardi G, Ramos Garcia L, Tenev T, Annibaldi A, Legrand A, Robertson D, et al. RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation. Mol Cell. 2019;73:413-428.e7 pubmed publisher
	Biase F, Wu Q, Calandrelli R, Rivas Astroza M, Zhou S, Chen Z, et al. Rainbow-Seq: Combining Cell Lineage Tracing with Single-Cell RNA Sequencing in Preimplantation Embryos. iScience. 2018;7:16-29 pubmed publisher
	Blengini C, Schindler K. Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation. Methods Mol Biol. 2018;1818:67-76 pubmed publisher
	Khushi M, Dean I, Teber E, Chircop M, Arthur J, Flores Rodriguez N. Automated classification and characterization of the mitotic spindle following knockdown of a mitosis-related protein. BMC Bioinformatics. 2017;18:566 pubmed publisher
	Shaimerdenova M, Karapina O, Mektepbayeva D, Alibek K, Akilbekova D. The effects of antiviral treatment on breast cancer cell line. Infect Agent Cancer. 2017;12:18 pubmed publisher
	Nguyen A, Gentilello A, Balboula A, Shrivastava V, Ohring J, Schindler K. Phosphorylation of threonine 3 on histone H3 by haspin kinase is required for meiosis I in mouse oocytes. J Cell Sci. 2014;127:5066-78 pubmed publisher
	Bender M, Stritt S, Nurden P, van Eeuwijk J, Zieger B, Kentouche K, et al. Megakaryocyte-specific Profilin1-deficiency alters microtubule stability and causes a Wiskott-Aldrich syndrome-like platelet defect. Nat Commun. 2014;5:4746 pubmed publisher
	Balboula A, Stein P, Schultz R, Schindler K. Knockdown of RBBP7 unveils a requirement of histone deacetylation for CPC function in mouse oocytes. Cell Cycle. 2014;13:600-11 pubmed publisher
	Gupta S, Braun A, Morowski M, Premsler T, Bender M, Nagy Z, et al. CLP36 is a negative regulator of glycoprotein VI signaling in platelets. Circ Res. 2012;111:1410-20 pubmed publisher

product information

Product Type :

Antibody

Product Name :

alpha Tubulin Monoclonal Antibody (B-5-1-2), Alexa Fluor 488

Catalog # :

322588

Quantity :

100 ug

Price :

US 562.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat, Sea urchin

Applications :

Immunocytochemistry: 2 ug/mL

Species :

Human, Mouse, Rat, Sea urchin

Clone :

B-5-1-2

Isotype :

IgG1, kappa

Storage :

Maintain refrigerated at 2-8 C for up to 1 month. For long term storage store at -20 C

Description :

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

Immunogen :

Sarkosyl-resistant filament from sea urchin sperm axonemes

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 2 ug/mL

Aliases :

&agr-tubulin 84B; 1t; a1t; ALPHA 84C; alpha tubulin; alpha tubulin 84B; alpha[[1]] tubulin; alpha[[1]]-tubulin; alpha1; alpha1 tubulin; alpha1t; alpha1tub; alpha1tub84B; alpha1-tubulin; alpha84B; alphaT; alphat-1; alphatub; alpha-tub; alphaTub1; alphatub84; alphaTUB84B; alpha-tub84B; alphaTub84B-PA; alphatubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; alpha-tubulin 4; Alpha-tubulin 6; alphaTubulin 84B; alpha-Tubulin 84B; alpha-Tubulin at 84B; alpha-tubulin I; Alpha-tubulin II; alpha-tubulin III; Alpha-tubulin isotype M-alpha-1; alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; alpha-tubulin isotype M-alpha-6; alpha-tubulin TUB1; alpha-tubulin ubiquitous; alphaTubulin84B; Alpha-Tubulin84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; AT25469p; aTub84B; a-tub84B; bA408E5.3; B-ALPHA-1; bcm948; BEST:LD32507; cb944; CG1913; CG1913-PA; chr3R:2914276. 2914416; clone 1.59; clone 2.30; Detyrosinated tubulin alpha-1A chain; Detyrosinated tubulin alpha-1B chain; Detyrosinated tubulin alpha-1C chain; Detyrosinated tubulin alpha-3 chain; Detyrosinated tubulin alpha-3C chain; Detyrosinated tubulin alpha-3D chain; Detyrosinated tubulin alpha-3E chain; DM1alpha; Dmel CG1913; Dmel_CG1913; DTA1; fb22g06; FLJ30169; H2 ALPHA; H2-ALPHA; hum-a-tub1; hum-a-tub2; I79_019110; K-ALPHA-1; l(3)84Bd; l(3)g3; lis3; LOC100726777; LOC100731885; LOC100857858; LOC101118307; LOC106557476; M[a]4; M[a]6; ms(3)nc33; nc33; RGD1565476; similar to alpha-tubulin isoform 1; similar to tubulin alpha 1; T; Talpha1; Talpha1 alpha-tubulin; TBA1_DROME; Testis-specific alpha-tubulin; Tub; Tub 84B; TUB1; Tub1C; Tub84B; TUBA1; Tuba-1; tuba1 protein; TUBA1/3/4; Tuba1a; tuba1a.L; tuba1a-a; tuba1a-b; TUBA1B; TUBA1C; tuba1l; tuba1l2; TUBA2; TUBA3; TUBA3C; TUBA3D; TUBA3E; Tuba4; Tuba4a; TUBA5; Tuba6; tuba8.S; tubA84B; tubal2; Tubalpha1; tubulin; tubulin 1 alpha-chain; tubulin 1alpha; tubulin alpha; tubulin alpha 1a; tubulin alpha 1a L homeolog; tubulin alpha 1b; tubulin alpha 1c; tubulin alpha 3; tubulin alpha 3c; tubulin alpha 3e; tubulin alpha 4a; tubulin alpha 6; tubulin alpha 8 S homeolog; tubulin alpha1; tubulin alpha-1 chain; tubulin alpha1 promoter; Tubulin alpha-1A chain; tubulin alpha-1B chain; Tubulin alpha-1C chain; tubulin alpha-1C chain-like; tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; tubulin alpha-3C/D chain; Tubulin alpha-3D chain; Tubulin alpha-3E chain; tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-5 chain; tubulin alpha-5 chain-like; tubulin alpha-6 chain; tubulin alpha-8 chain; tubulin alpha-ubiquitous chain; tubulin B-alpha-1; Tubulin H2-alpha; tubulin K-alpha-1; tubulin(alpha1); tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1, like; tubulin, alpha 1, like 2; tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 5; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin1alpha; tubulin84B; Tubulin-alpha1; Unknown (protein for MGC:133894); unnamed protein product; wu:fb22g06; wu:fb37a10; XELAEV_18019849mg; XELAEV_18020901mg; YML085C

more info or order :

Invitrogen product webpage