Desmoglein 3 Monoclonal Antibody (5G11) | Invitrogen 32-6300 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Desmoglein 3 Monoclonal Antibody (5G11)

catalog :

32-6300

quantity :

100 µg

price :

US 398.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5G11

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 36

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:500; loading ...; fig 1b	Shelton W, Thomas S, Alexander H, Thomes C, Conway D, Dubash A. Desmoglein-2 harnesses a PDZ-GEF2/Rap1 signaling axis to control cell spreading and focal adhesions independent of cell-cell adhesion. Sci Rep. 2021;11:13295 pubmed publisher
flow cytometry; human; loading ...; fig 2c	Ebert L, Tan L, Johan M, Min K, Cockshell M, Parham K, et al. A non-canonical role for desmoglein-2 in endothelial cells: implications for neoangiogenesis. Angiogenesis. 2016;19:463-86 pubmed publisher
immunocytochemistry; mouse western blot; mouse	Hartlieb E, Rötzer V, Radeva M, Spindler V, Waschke J. Desmoglein 2 compensates for desmoglein 3 but does not control cell adhesion via regulation of p38 mitogen-activated protein kinase in keratinocytes. J Biol Chem. 2014;289:17043-53 pubmed publisher
immunocytochemistry; human	Rötzer V, Breit A, Waschke J, Spindler V. Adducin is required for desmosomal cohesion in keratinocytes. J Biol Chem. 2014;289:14925-40 pubmed publisher
western blot; human	Stahley S, Saito M, Faundez V, Koval M, Mattheyses A, Kowalczyk A. Desmosome assembly and disassembly are membrane raft-dependent. PLoS ONE. 2014;9:e87809 pubmed publisher
immunohistochemistry; human	Pontiggia L, Biedermann T, Böttcher Haberzeth S, Oliveira C, Braziulis E, Klar A, et al. De novo epidermal regeneration using human eccrine sweat gland cells: higher competence of secretory over absorptive cells. J Invest Dermatol. 2014;134:1735-1742 pubmed publisher
immunohistochemistry - paraffin section; mouse	Chen Y, Lee L, Chao Y, Chang J, Lu Y, Li H, et al. DSG3 facilitates cancer cell growth and invasion through the DSG3-plakoglobin-TCF/LEF-Myc/cyclin D1/MMP signaling pathway. PLoS ONE. 2013;8:e64088 pubmed publisher
immunocytochemistry; human; fig 3 western blot; human; fig 3	Mao X, Li H, Sano Y, Gaestel M, Mo Park J, Payne A. MAPKAP kinase 2 (MK2)-dependent and -independent models of blister formation in pemphigus vulgaris. J Invest Dermatol. 2014;134:68-76 pubmed publisher
immunohistochemistry; human; fig 1	Patel V, Martin D, Malhotra R, Marsh C, DoÃ§i C, Veenstra T, et al. DSG3 as a biomarker for the ultrasensitive detection of occult lymph node metastasis in oral cancer using nanostructured immunoarrays. Oral Oncol. 2013;49:93-101 pubmed publisher
immunohistochemistry; human; 1:40	Wex T, Kuester D, Monkemuller K, Stahr A, Fry L, Kandulski A, et al. Assessment of desmosomal components (desmoglein 1-3, plakoglobin) in cardia mucosa in relation to gastroesophageal reflux disease and Helicobacter pylori infection. Hum Pathol. 2012;43:1745-54 pubmed publisher
immunohistochemistry; human; 1:40; fig 1	Wex T, Monkemuller K, Stahr A, Kuester D, Fry L, Völkel S, et al. Gastro-oesophageal reflux disease is associated with up-regulation of desmosomal components in oesophageal mucosa. Histopathology. 2012;60:405-15 pubmed publisher
immunohistochemistry; mouse; fig 2 immunocytochemistry; human; fig 3	Mao X, Sano Y, Park J, Payne A. p38 MAPK activation is downstream of the loss of intercellular adhesion in pemphigus vulgaris. J Biol Chem. 2011;286:1283-91 pubmed publisher
immunocytochemistry; human; fig 3 immunohistochemistry; human; fig 1 western blot; human; fig 2	Mao X, Nagler A, Farber S, Choi E, Jackson L, Leiferman K, et al. Autoimmunity to desmocollin 3 in pemphigus vulgaris. Am J Pathol. 2010;177:2724-30 pubmed publisher
immunocytochemistry; human; 1:100	Schlegel N, Meir M, Heupel W, Holthöfer B, Leube R, Waschke J. Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity. Am J Physiol Gastrointest Liver Physiol. 2010;298:G774-83 pubmed publisher
immunohistochemistry - paraffin section; human; 1:350	Sen G, Reuter J, Webster D, Zhu L, Khavari P. DNMT1 maintains progenitor function in self-renewing somatic tissue. Nature. 2010;463:563-7 pubmed publisher
immunohistochemistry; human; 1:25; fig 1	Yeung A, Tint N, Kulkarni B, Mohammed I, Suleman H, Hopkinson A, et al. Infant limbus: an immunohistological study. Exp Eye Res. 2009;88:1161-4 pubmed publisher
western blot; human; fig 5	Mao X, Choi E, Payne A. Disruption of desmosome assembly by monovalent human pemphigus vulgaris monoclonal antibodies. J Invest Dermatol. 2009;129:908-18 pubmed publisher
immunoprecipitation; human; fig 2	Kanno M, Aoyama Y, Isa Y, Yamamoto Y, Kitajima Y. P120 catenin is associated with desmogleins when desmosomes are assembled in high-Ca2+ medium but not when disassembled in low-Ca2+ medium in DJM-1 cells. J Dermatol. 2008;35:317-24 pubmed publisher
immunohistochemistry - frozen section; human	Schlötzer Schrehardt U, Dietrich T, Saito K, Sorokin L, Sasaki T, Paulsson M, et al. Characterization of extracellular matrix components in the limbal epithelial stem cell compartment. Exp Eye Res. 2007;85:845-60 pubmed
immunohistochemistry; human; fig 9	Chen Y, Chang J, Lee L, Wang H, Liao C, Chiu C, et al. DSG3 is overexpressed in head neck cancer and is a potential molecular target for inhibition of oncogenesis. Oncogene. 2007;26:467-76 pubmed
immunohistochemistry - paraffin section; human; 1:100; tbl 1	Yang L, Shirakata Y, Shudou M, Dai X, Tokumaru S, Hirakawa S, et al. New skin-equivalent model from de-epithelialized amnion membrane. Cell Tissue Res. 2006;326:69-77 pubmed
immunohistochemistry - paraffin section; human; 1:30	Chung C, Parker J, Karaca G, Wu J, Funkhouser W, Moore D, et al. Molecular classification of head and neck squamous cell carcinomas using patterns of gene expression. Cancer Cell. 2004;5:489-500 pubmed
immunohistochemistry - paraffin section; human; tbl 1	Kurzen H, Münzing I, Hartschuh W. Expression of desmosomal proteins in squamous cell carcinomas of the skin. J Cutan Pathol. 2003;30:621-30 pubmed
	Yang Q, Pinto V, Duan W, Paxton E, Dessauer J, Ryan W, et al. In vitro Characteristics of Heterogeneous Equine Hoof Progenitor Cell Isolates. Front Bioeng Biotechnol. 2019;7:155 pubmed publisher
	Domke L, Franke W. The cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions-a novel architectonic cell-cell junction system. Cell Tissue Res. 2019;375:451-482 pubmed publisher
	Funahashi S, Kawai S, Fujii E, Taniguchi K, Nakano K, Ishikawa S, et al. Generation of an anti-desmoglein 3 antibody without pathogenic activity of pemphigus vulgaris for therapeutic application to squamous cell carcinoma. J Biochem. 2018;164:471-481 pubmed publisher
	Zhang C, Goldscheider I, Ruzicka T, Sárdy M. Pemphigus Vulgaris Persistently Localized to the Nose with Local and Systemic Response to Topical Steroids. Acta Derm Venereol. 2017;97:1136-1137 pubmed publisher
	Mao X, Cho M, Ellebrecht C, Mukherjee E, Payne A. Stat3 regulates desmoglein 3 transcription in epithelial keratinocytes. JCI Insight. 2017;2: pubmed publisher
	Nubile M, Curcio C, Dua H, Calienno R, Lanzini M, Iezzi M, et al. Pathological changes of the anatomical structure and markers of the limbal stem cell niche due to inflammation. Mol Vis. 2013;19:516-25 pubmed
	Hartlieb E, Kempf B, Partilla M, Vigh B, Spindler V, Waschke J. Desmoglein 2 is less important than desmoglein 3 for keratinocyte cohesion. PLoS ONE. 2013;8:e53739 pubmed publisher
	Raiko L, Siljamäki E, Mahoney M, Putaala H, Suominen E, Peltonen J, et al. Hailey-Hailey disease and tight junctions: Claudins 1 and 4 are regulated by ATP2C1 gene encoding Ca(2+) /Mn(2+) ATPase SPCA1 in cultured keratinocytes. Exp Dermatol. 2012;21:586-91 pubmed publisher
	Biedermann T, Pontiggia L, Böttcher Haberzeth S, Tharakan S, Braziulis E, Schiestl C, et al. Human eccrine sweat gland cells can reconstitute a stratified epidermis. J Invest Dermatol. 2010;130:1996-2009 pubmed publisher
	Jolly P, Berkowitz P, Bektas M, Lee H, Chua M, Diaz L, et al. p38MAPK signaling and desmoglein-3 internalization are linked events in pemphigus acantholysis. J Biol Chem. 2010;285:8936-41 pubmed publisher
	Cirillo N, Gombos F, Lanza A. Pemphigus vulgaris immunoglobulin G can recognize a 130 000 MW antigen other than desmoglein 3 on peripheral blood mononuclear cell surface. Immunology. 2007;121:377-82 pubmed
	Calkins C, Setzer S, Jennings J, Summers S, Tsunoda K, Amagai M, et al. Desmoglein endocytosis and desmosome disassembly are coordinated responses to pemphigus autoantibodies. J Biol Chem. 2006;281:7623-34 pubmed
	Payne A, Ishii K, Kacir S, Lin C, Li H, Hanakawa Y, et al. Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. J Clin Invest. 2005;115:888-99 pubmed

product information

Product Type :

Antibody

Product Name :

Desmoglein 3 Monoclonal Antibody (5G11)

Catalog # :

32-6300

Quantity :

100 µg

Price :

US 398.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

Immunohistochemistry: 20-50 µg/mL, Immunoprecipitation: 5-10 µg, Western Blot: 1-3 µg/mL

Species :

Human

Clone :

5G11

Isotype :

IgG1

Storage :

-20°C

Description :

Desmoglein 3 (Dsg3), also known as Cadherin family member 6 (CDHF6), is a member of the desmosomal cadherin family and plays a critical role in cell-cell adhesion. It is predominately expressed in stratified squamous epithelium, including the skin, oral mucosa, and esophagus. Desmoglein 3 has been characterized as the auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease. Binding of anti-Desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.

Immunogen :

Human desmoglein-3 extracellular domain.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry: 20-50 µg/mL, Immunoprecipitation: 5-10 µg, Western Blot: 1-3 µg/mL

Aliases :

130 kDa pemphigus vulgaris antigen; 130 kDa pemphigus vulgaris antigen homolog; bal; balding; Cadherin family member 6; CDHF6; desmoglein 3; desmoglein-3; DKFZp686P23184; Dsg3; PVA

more info or order :

Invitrogen product webpage