Claudin 2 Monoclonal Antibody (12H12) | Invitrogen 32-5600 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Claudin 2 Monoclonal Antibody (12H12)

catalog :

32-5600

quantity :

100 µg

price :

US 404.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

12H12

reactivity :

American opossums, African green monkey, domestic goat, human, mouse, rat, dogs, pigs , rhesus macaque

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation

more info or order :

Invitrogen product webpage

citations: 99

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; 1:5000; loading ...; fig 2a immunohistochemistry; human; 1:250; loading ...; fig 3a, 5d, s1	Tabariès S, Annis M, Lazaris A, Petrillo S, Huxham J, Abdellatif A, et al. Claudin-2 promotes colorectal cancer liver metastasis and is a biomarker of the replacement type growth pattern. Commun Biol. 2021;4:657 pubmed publisher
immunocytochemistry; dogs; fig 2 western blot; dogs; fig 2	Tokuda S, Hirai T, Furuse M. Claudin-4 knockout by TALEN-mediated gene targeting in MDCK cells: Claudin-4 is dispensable for the permeability properties of tight junctions in wild-type MDCK cells. PLoS ONE. 2017;12:e0182521 pubmed publisher
immunohistochemistry - paraffin section; dogs; 1:200; loading ...; fig 2b western blot; dogs; 1:1000; loading ...; fig 1f	Ahn C, Shin D, Lee D, Kang S, Seok J, Kang H, et al. Expression of claudins, occludin, junction adhesion molecule A and zona occludens 1 in canine organs. Mol Med Rep. 2016;14:3697-703 pubmed publisher
immunohistochemistry - paraffin section; human; fig 4E immunocytochemistry; human; fig 2E western blot; human; fig 1	Randall K, Henderson N, Reens J, Eckersley S, Nyström A, South M, et al. Claudin-2 Expression Levels in Ulcerative Colitis: Development and Validation of an In-Situ Hybridisation Assay for Therapeutic Studies. PLoS ONE. 2016;11:e0162076 pubmed publisher
immunohistochemistry; mouse; 7 ug/ml; loading ...; tbl s5 western blot; mouse; 3 ug/ml; loading ...; tbl s5	de Sousa Rodrigues M, Bekhbat M, Houser M, Chang J, Walker D, Jones D, et al. Chronic psychological stress and high-fat high-fructose diet disrupt metabolic and inflammatory gene networks in the brain, liver, and gut and promote behavioral deficits in mice. Brain Behav Immun. 2017;59:158-172 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 3a	Ocón B, Aranda C, Gámez Belmonte R, Suárez M, Zarzuelo A, Martinez Augustin O, et al. The glucocorticoid budesonide has protective and deleterious effects in experimental colitis in mice. Biochem Pharmacol. 2016;116:73-88 pubmed publisher
western blot; rhesus macaque; 2 ug/ml; loading ...; fig 9b	Shea Donohue T, Fasano A, Zhao A, Notari L, Yan S, Sun R, et al. Mechanisms Involved in the Development of the Chronic Gastrointestinal Syndrome in Nonhuman Primates after Total-Body Irradiation with Bone Marrow Shielding. Radiat Res. 2016;185:591-603 pubmed publisher
western blot; domestic goat; 1:1000; fig 3	Elfers K, Marr I, Wilkens M, Breves G, Langeheine M, Brehm R, et al. Expression of Tight Junction Proteins and Cadherin 17 in the Small Intestine of Young Goats Offered a Reduced N and/or Ca Diet. PLoS ONE. 2016;11:e0154311 pubmed publisher
immunohistochemistry - paraffin section; mouse; fig 1	Gu L, Cong J, Zhang J, Tian Y, Zhai X. A microwave antigen retrieval method using two heating steps for enhanced immunostaining on aldehyde-fixed paraffin-embedded tissue sections. Histochem Cell Biol. 2016;145:675-80 pubmed publisher
western blot; human	de ValliÃ¨re C, Vidal S, Clay I, Jurisic G, Tcymbarevich I, Lang S, et al. The pH-sensing receptor OGR1 improves barrier function of epithelial cells and inhibits migration in an acidic environment. Am J Physiol Gastrointest Liver Physiol. 2015;309:G475-90 pubmed publisher
immunocytochemistry; dogs; fig 3g western blot; dogs; fig 3f	Van Itallie C, Tietgens A, Krystofiak E, Kachar B, Anderson J. A complex of ZO-1 and the BAR-domain protein TOCA-1 regulates actin assembly at the tight junction. Mol Biol Cell. 2015;26:2769-87 pubmed publisher
western blot; mouse; 0.1 ug/ml; fig 1 western blot; human; 0.1 ug/ml; fig 1	TabariÃ¨s S, Annis M, Hsu B, Tam C, Savage P, Park M, et al. Lyn modulates Claudin-2 expression and is a therapeutic target for breast cancer liver metastasis. Oncotarget. 2015;6:9476-87 pubmed
immunohistochemistry; rat; 1:2000; fig 9	FernÃ¡ndez Blanco J, EstÃ©vez J, Shea Donohue T, MartÃnez V, Vergara P. Changes in Epithelial Barrier Function in Response to Parasitic Infection: Implications for IBD Pathogenesis. J Crohns Colitis. 2015;9:463-76 pubmed publisher
western blot; human; 1:1000	Contreras T, Ricciardi E, Cremonini E, Oteiza P. (-)-Epicatechin in the prevention of tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity. Arch Biochem Biophys. 2015;573:84-91 pubmed publisher
immunocytochemistry; mouse	Freeman J, Feng Y, Demehri F, Dempsey P, Teitelbaum D. TPN-associated intestinal epithelial cell atrophy is modulated by TLR4/EGF signaling pathways. FASEB J. 2015;29:2943-58 pubmed publisher
immunocytochemistry; dogs western blot; dogs	Tokuda S, Furuse M. Claudin-2 knockout by TALEN-mediated gene targeting in MDCK cells: claudin-2 independently determines the leaky property of tight junctions in MDCK cells. PLoS ONE. 2015;10:e0119869 pubmed publisher
western blot; human	Watari A, Hashegawa M, Yagi K, Kondoh M. Homoharringtonine increases intestinal epithelial permeability by modulating specific claudin isoforms in Caco-2 cell monolayers. Eur J Pharm Biopharm. 2015;89:232-8 pubmed publisher
immunohistochemistry; human; 1:100; fig 1	Ma F, Ding X, Fan Y, Ying J, Zheng S, Lu N, et al. A CLDN1-negative phenotype predicts poor prognosis in triple-negative breast cancer. PLoS ONE. 2014;9:e112765 pubmed publisher
flow cytometry; human	Landy J, Al Hassi H, Ronde E, English N, Mann E, Bernardo D, et al. Innate immune factors in the development and maintenance of pouchitis. Inflamm Bowel Dis. 2014;20:1942-9 pubmed publisher
immunocytochemistry; dogs western blot; dogs	Tokuda S, Higashi T, Furuse M. ZO-1 knockout by TALEN-mediated gene targeting in MDCK cells: involvement of ZO-1 in the regulation of cytoskeleton and cell shape. PLoS ONE. 2014;9:e104994 pubmed publisher
immunocytochemistry; human; fig 3	Ragupathy S, Esmaeili F, Paschoud S, Sublet E, Citi S, Borchard G. Toll-like receptor 2 regulates the barrier function of human bronchial epithelial monolayers through atypical protein kinase C zeta, and an increase in expression of claudin-1. Tissue Barriers. 2014;2:e29166 pubmed publisher
western blot; human	Crespi A, Bertoni A, Ferrari I, Padovano V, Della Mina P, Berti E, et al. POF1B localizes to desmosomes and regulates cell adhesion in human intestinal and keratinocyte cell lines. J Invest Dermatol. 2015;135:192-201 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; fig 1b	Virman J, Soini Y, Kujala P, Luukkaala T, Salminen T, Sunela K, et al. Claudins as prognostic factors for renal cell cancer. Anticancer Res. 2014;34:4181-7 pubmed
immunocytochemistry; human; 1:100; fig 4	Glotfelty L, Zahs A, Hodges K, Shan K, Alto N, Hecht G. Enteropathogenic E. coli effectors EspG1/G2 disrupt microtubules, contribute to tight junction perturbation and inhibit restoration. Cell Microbiol. 2014;16:1767-83 pubmed publisher
immunocytochemistry; African green monkey western blot; African green monkey	García Hernández V, Flores Maldonado C, Rincon Heredia R, Verdejo Torres O, Bonilla Delgado J, Meneses Morales I, et al. EGF regulates claudin-2 and -4 expression through Src and STAT3 in MDCK cells. J Cell Physiol. 2015;230:105-15 pubmed publisher
western blot; mouse; loading ...; fig 6b	Gerlach K, Hwang Y, Nikolaev A, Atreya R, Dornhoff H, Steiner S, et al. TH9 cells that express the transcription factor PU.1 drive T cell-mediated colitis via IL-9 receptor signaling in intestinal epithelial cells. Nat Immunol. 2014;15:676-86 pubmed publisher
immunocytochemistry; human; 1:300 western blot; human; 1:500	Wilmes A, Aschauer L, Limonciel A, Pfaller W, Jennings P. Evidence for a role of claudin 2 as a proximal tubular stress responsive paracellular water channel. Toxicol Appl Pharmacol. 2014;279:163-72 pubmed publisher
immunohistochemistry; human western blot; human; 1:5000	Sambrotta M, Strautnieks S, Papouli E, Rushton P, Clark B, Parry D, et al. Mutations in TJP2 cause progressive cholestatic liver disease. Nat Genet. 2014;46:326-8 pubmed publisher
immunocytochemistry; human; fig 3 western blot; human; 0.8 ug/ml; fig 3	Aschauer L, Gruber L, Pfaller W, Limonciel A, Athersuch T, Cavill R, et al. Delineation of the key aspects in the regulation of epithelial monolayer formation. Mol Cell Biol. 2013;33:2535-50 pubmed publisher
immunohistochemistry - paraffin section; human immunohistochemistry - frozen section; mouse; 2.5 ug/ml; fig 4 western blot; mouse	Bergmann K, Liu S, Tian R, Kushnir A, Turner J, Li H, et al. Bifidobacteria stabilize claudins at tight junctions and prevent intestinal barrier dysfunction in mouse necrotizing enterocolitis. Am J Pathol. 2013;182:1595-606 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 3	Kissoon Singh V, Moreau F, Trusevych E, Chadee K. Entamoeba histolytica exacerbates epithelial tight junction permeability and proinflammatory responses in Muc2(-/-) mice. Am J Pathol. 2013;182:852-65 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50; fig 1	Lappi Blanco E, Lehtonen S, Sormunen R, Merikallio H, Soini Y, Kaarteenaho R. Divergence of tight and adherens junction factors in alveolar epithelium in pulmonary fibrosis. Hum Pathol. 2013;44:895-907 pubmed publisher
immunohistochemistry - frozen section; human; 1:100; fig 2 western blot; human; fig 2	Whitcomb D, Larusch J, Krasinskas A, Klei L, Smith J, Brand R, et al. Common genetic variants in the CLDN2 and PRSS1-PRSS2 loci alter risk for alcohol-related and sporadic pancreatitis. Nat Genet. 2012;44:1349-54 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:250; fig 4 immunoprecipitation; mouse; fig 2 western blot; mouse; 1:500; fig 2	Lei Z, Maeda T, Tamura A, Nakamura T, Yamazaki Y, Shiratori H, et al. EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins. Dev Biol. 2012;371:136-45 pubmed publisher
immunocytochemistry; human; 1:300; fig 4 western blot; human; 1:600; fig 6	Limonciel A, Wilmes A, Aschauer L, Radford R, Bloch K, McMorrow T, et al. Oxidative stress induced by potassium bromate exposure results in altered tight junction protein expression in renal proximal tubule cells. Arch Toxicol. 2012;86:1741-51 pubmed publisher
immunohistochemistry - frozen section; human; 1:100 western blot; human; 1:1000; fig 2	Ghule V, Gray C, Galimberti A, Anumba D. Prostaglandin-induced cervical remodelling in humans in the first trimester is associated with increased expression of specific tight junction, but not gap junction proteins. J Transl Med. 2012;10:40 pubmed publisher
immunocytochemistry; dogs; fig 1 western blot; dogs; fig 2, 4	Dukes J, Whitley P, Chalmers A. The PIKfyve inhibitor YM201636 blocks the continuous recycling of the tight junction proteins claudin-1 and claudin-2 in MDCK cells. PLoS ONE. 2012;7:e28659 pubmed publisher
western blot; dogs; fig 1	Paschoud S, Guillemot L, Citi S. Distinct domains of paracingulin are involved in its targeting to the actin cytoskeleton and regulation of apical junction assembly. J Biol Chem. 2012;287:13159-69 pubmed publisher
flow cytometry; mouse; fig 3 western blot; mouse; fig s1	Van den Bossche J, Laoui D, Morias Y, Movahedi K, Raes G, De Baetselier P, et al. Claudin-1, claudin-2 and claudin-11 genes differentially associate with distinct types of anti-inflammatory macrophages in vitro and with parasite- and tumour-elicited macrophages in vivo. Scand J Immunol. 2012;75:588-98 pubmed publisher
immunocytochemistry; dogs; 1:25; fig s7	Dukes J, Fish L, Richardson J, Blaikley E, Burns S, Caunt C, et al. Functional ESCRT machinery is required for constitutive recycling of claudin-1 and maintenance of polarity in vertebrate epithelial cells. Mol Biol Cell. 2011;22:3192-205 pubmed publisher
immunohistochemistry - paraffin section; human; fig 3	Kojima F, Ishida M, Takikita Suzuki M, Hotta M, Katsura K, Nagata A, et al. Claudin expression profiles in Epstein-Barr virus-associated nasopharyngeal carcinoma. Oncol Rep. 2010;23:927-31 pubmed
western blot; mouse; 1:100; fig 10	Cattin A, Le Beyec J, Barreau F, Saint Just S, Houllier A, Gonzalez F, et al. Hepatocyte nuclear factor 4alpha, a key factor for homeostasis, cell architecture, and barrier function of the adult intestinal epithelium. Mol Cell Biol. 2009;29:6294-308 pubmed publisher
immunocytochemistry; rat; 1:100; tbl 3	Son S, Kojima T, Decaens C, Yamaguchi H, Ito T, Imamura M, et al. Knockdown of tight junction protein claudin-2 prevents bile canalicular formation in WIF-B9 cells. Histochem Cell Biol. 2009;131:411-24 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; fig 2	Ishida M, Kushima R, Okabe H. Claudin expression in rectal well-differentiated endocrine neoplasms (carcinoid tumors). Oncol Rep. 2009;21:113-7 pubmed
immunohistochemistry - paraffin section; human; loading ...; fig 1b	Kaarteenaho Wiik R, Soini Y. Claudin-1, -2, -3, -4, -5, and -7 in usual interstitial pneumonia and sarcoidosis. J Histochem Cytochem. 2009;57:187-95 pubmed publisher
western blot; human; fig 1	Sugimoto M, Inoko A, Shiromizu T, Nakayama M, Zou P, Yonemura S, et al. The keratin-binding protein Albatross regulates polarization of epithelial cells. J Cell Biol. 2008;183:19-28 pubmed publisher
immunohistochemistry - paraffin section; human; fig 1	Weber C, Nalle S, Tretiakova M, Rubin D, Turner J. Claudin-1 and claudin-2 expression is elevated in inflammatory bowel disease and may contribute to early neoplastic transformation. Lab Invest. 2008;88:1110-20 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; fig 3	Kim T, Huh J, Lee S, Kang H, Kim G, An H. Down-regulation of claudin-2 in breast carcinomas is associated with advanced disease. Histopathology. 2008;53:48-55 pubmed publisher
western blot; dogs; fig 3	Paschoud S, Citi S. Inducible overexpression of cingulin in stably transfected MDCK cells does not affect tight junction organization and gene expression. Mol Membr Biol. 2008;25:1-13 pubmed
immunocytochemistry; rat; 1:100; fig 2B	Kojima T, Takano K, Yamamoto T, Murata M, Son S, Imamura M, et al. Transforming growth factor-beta induces epithelial to mesenchymal transition by down-regulation of claudin-1 expression and the fence function in adult rat hepatocytes. Liver Int. 2008;28:534-45 pubmed
immunohistochemistry - paraffin section; human; 1:400; fig 4	Audard V, Grimber G, Elie C, Radenen B, Audebourg A, Letourneur F, et al. Cholestasis is a marker for hepatocellular carcinomas displaying beta-catenin mutations. J Pathol. 2007;212:345-52 pubmed
immunocytochemistry; dogs; fig 4 western blot; dogs; fig 1	Guillemot L, Citi S. Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA. Mol Biol Cell. 2006;17:3569-77 pubmed
immunohistochemistry - paraffin section; human; 1:50	Soini Y, Kinnula V, Kahlos K, Paakko P. Claudins in differential diagnosis between mesothelioma and metastatic adenocarcinoma of the pleura. J Clin Pathol. 2006;59:250-4 pubmed
immunocytochemistry; human immunocytochemistry; pigs immunocytochemistry; American opossums immunocytochemistry; rat	Prozialeck W, Edwards J, Lamar P, Smith C. Epithelial barrier characteristics and expression of cell adhesion molecules in proximal tubule-derived cell lines commonly used for in vitro toxicity studies. Toxicol In Vitro. 2006;20:942-53 pubmed
	Tratnjek L, Sibinovska N, Kralj S, Makovec D, Kristan K, Kreft M. Standardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing. Sci Rep. 2021;11:6664 pubmed publisher
	Caron T, Scott K, Sinha N, Muthupalani S, Baqai M, Ang L, et al. Claudin-18 Loss Alters Transcellular Chloride Flux but not Tight Junction Ion Selectivity in Gastric Epithelial Cells. Cell Mol Gastroenterol Hepatol. 2021;11:783-801 pubmed publisher
	Le N, Mazahery C, Nguyen K, Levine A. Regulation of Intestinal Epithelial Barrier and Immune Function by Activated T Cells. Cell Mol Gastroenterol Hepatol. 2021;11:55-76 pubmed publisher
	Smith N, Saunders D, Lerner M, Zalles M, Mamedova N, Cheong D, et al. In vivo and ex vivo assessment of bladder hyper-permeability and using molecular targeted magnetic resonance imaging to detect claudin-2 in a mouse model for interstitial cystitis. PLoS ONE. 2020;15:e0239282 pubmed publisher
	Cai T, Ye X, Li R, Chen H, Wang Y, Yong H, et al. Resveratrol Modulates the Gut Microbiota and Inflammation to Protect Against Diabetic Nephropathy in Mice. Front Pharmacol. 2020;11:1249 pubmed publisher
	Shi Y, Cui X, Sun Y, Zhao Q, Liu T. Intestinal vitamin D receptor signaling ameliorates dextran sulfate sodium-induced colitis by suppressing necroptosis of intestinal epithelial cells. FASEB J. 2020;34:13494-13506 pubmed publisher
	Markov A, Fedorova A, Kravtsova V, Bikmurzina A, Okorokova L, Matchkov V, et al. Circulating Ouabain Modulates Expression of Claudins in Rat Intestine and Cerebral Blood Vessels. Int J Mol Sci. 2020;21: pubmed publisher
	Shi Y, Liu Z, Cui X, Zhao Q, Liu T. Intestinal vitamin D receptor knockout protects from oxazolone-induced colitis. Cell Death Dis. 2020;11:461 pubmed publisher
	Raju P, Shashikanth N, Tsai P, Pongkorpsakol P, Chánez Paredes S, Steinhagen P, et al. Inactivation of paracellular cation-selective claudin-2 channels attenuates immune-mediated experimental colitis in mice. J Clin Invest. 2020;130:5197-5208 pubmed publisher
	Price G, Chadjichristos C, Kavvadas P, Tang S, Yiu W, Green C, et al. Blocking Connexin-43 mediated hemichannel activity protects against early tubular injury in experimental chronic kidney disease. Cell Commun Signal. 2020;18:79 pubmed publisher
	Plain A, Pan W, O Neill D, Ure M, Beggs M, Farhan M, et al. Claudin-12 Knockout Mice Demonstrate Reduced Proximal Tubule Calcium Permeability. Int J Mol Sci. 2020;21: pubmed publisher
	Curry J, Tokuda S, McAnulty P, Yu A. Combinatorial expression of claudins in the proximal renal tubule and its functional consequences. Am J Physiol Renal Physiol. 2020;318:F1138-F1146 pubmed publisher
	Curry J, Saurette M, Askari M, Pei L, Filla M, Beggs M, et al. Claudin-2 deficiency associates with hypercalciuria in mice and human kidney stone disease. J Clin Invest. 2020;130:1948-1960 pubmed publisher
	Hoerscher A, Horné F, Dietze R, Berkes E, Oehmke F, Tinneberg H, et al. Localization of claudin-2 and claudin-3 in eutopic and ectopic endometrium is highly similar. Arch Gynecol Obstet. 2020;301:1003-1011 pubmed publisher
	Helm E, Curry S, De Mille C, Schweer W, Burrough E, Gabler N. Impact of viral disease hypophagia on pig jejunal function and integrity. PLoS ONE. 2020;15:e0227265 pubmed publisher
	Sayoc Becerra A, Krishnan M, Fan S, Jimenez J, HERNANDEZ R, Gibson K, et al. The JAK-Inhibitor Tofacitinib Rescues Human Intestinal Epithelial Cells and Colonoids from Cytokine-Induced Barrier Dysfunction. Inflamm Bowel Dis. 2020;26:407-422 pubmed publisher
	Otani T, Nguyen T, Tokuda S, Sugihara K, Sugawara T, Furuse K, et al. Claudins and JAM-A coordinately regulate tight junction formation and epithelial polarity. J Cell Biol. 2019;218:3372-3396 pubmed publisher
	Luo Y, Xu J, Zhang C, Jiang C, Ma Y, He H, et al. Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4+ ETEC infection. Vet Res. 2019;50:48 pubmed publisher
	Van Itallie C, Lidman K, Tietgens A, Anderson J. Newly synthesized claudins but not occludin are added to the basal side of the tight junction. Mol Biol Cell. 2019;30:1406-1424 pubmed publisher
	Tabariès S, McNulty A, Ouellet V, Annis M, Dessureault M, Vinette M, et al. Afadin cooperates with Claudin-2 to promote breast cancer metastasis. Genes Dev. 2019;33:180-193 pubmed publisher
	Sartor G, Malvezzi A, Kumar A, Andrade N, Wiedner H, Vilca S, et al. Enhancement of BDNF Expression and Memory by HDAC Inhibition Requires BET Bromodomain Reader Proteins. J Neurosci. 2019;39:612-626 pubmed publisher
	Canuto L, Collares Buzato C. Increased osmolality enhances the tight junction-mediated barrier function in a cultured renal epithelial cell line. Cell Biol Int. 2019;43:73-82 pubmed publisher
	Chen H, Lu R, Zhang Y, Sun J. Vitamin D Receptor Deletion Leads to the Destruction of Tight and Adherens Junctions in Lungs. Tissue Barriers. 2018;6:1-13 pubmed publisher
	Granados Soler J, Junginger J, Hewicker Trautwein M, Bornemann Kolatzki K, Beck J, Brenig B, et al. TiHo-0906: a new feline mammary cancer cell line with molecular, morphological, and immunocytological characteristics of epithelial to mesenchymal transition. Sci Rep. 2018;8:13231 pubmed publisher
	Ahmad R, Kumar B, Pan K, Dhawan P, Singh A. HDAC-4 regulates claudin-2 expression in EGFR-ERK1/2 dependent manner to regulate colonic epithelial cell differentiation. Oncotarget. 2017;8:87718-87736 pubmed publisher
	Mezheyeuski A, Strell C, Hrynchyk I, Guren T, Dragomir A, Doroshenko T, et al. Treatment-related survival associations of claudin-2 expression in fibroblasts of colorectal cancer. Virchows Arch. 2018;472:395-405 pubmed publisher
	Cuellar P, Hernández Nava E, García Rivera G, Chavez Munguia B, Schnoor M, Betanzos A, et al. Entamoeba histolytica EhCP112 Dislocates and Degrades Claudin-1 and Claudin-2 at Tight Junctions of the Intestinal Epithelium. Front Cell Infect Microbiol. 2017;7:372 pubmed publisher
	Ahmad R, Rah B, Bastola D, Dhawan P, Singh A. Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching. Sci Rep. 2017;7:5125 pubmed publisher
	Tokuda S, Hirai T, Furuse M. Effects of Osmolality on Paracellular Transport in MDCK II Cells. PLoS ONE. 2016;11:e0166904 pubmed publisher
	Sabui S, Bohl J, Kapadia R, Cogburn K, Ghosal A, Lambrecht N, et al. Role of the sodium-dependent multivitamin transporter (SMVT) in the maintenance of intestinal mucosal integrity. Am J Physiol Gastrointest Liver Physiol. 2016;311:G561-70 pubmed publisher
	Pei L, Solis G, Nguyen M, Kamat N, Magenheimer L, Zhuo M, et al. Paracellular epithelial sodium transport maximizes energy efficiency in the kidney. J Clin Invest. 2016;126:2509-18 pubmed publisher
	Tokuda S, Kim Y, Matsumoto H, Muro S, Hirai T, Mishima M, et al. Effects of Hydrostatic Pressure on Carcinogenic Properties of Epithelia. PLoS ONE. 2015;10:e0145522 pubmed publisher
	Amoozadeh Y, Dan Q, Xiao J, Waheed F, SzÃ¡szi K. Tumor necrosis factor-Î± induces a biphasic change in claudin-2 expression in tubular epithelial cells: role in barrier functions. Am J Physiol Cell Physiol. 2015;309:C38-50 pubmed publisher
	Hintsala H, Siponen M, Haapasaari K, Karihtala P, Soini Y. Claudins 1, 2, 3, 4, 5 and 7 in solar keratosis and squamocellular carcinoma of the skin. Int J Clin Exp Pathol. 2013;6:2855-63 pubmed
	Fu Y, Huang B, Shi Z, Han J, Wang Y, Huangfu J, et al. SRSF1 and SRSF9 RNA binding proteins promote Wnt signalling-mediated tumorigenesis by enhancing ?-catenin biosynthesis. EMBO Mol Med. 2013;5:737-50 pubmed publisher
	Van Itallie C, Aponte A, Tietgens A, Gucek M, Fredriksson K, Anderson J. The N and C termini of ZO-1 are surrounded by distinct proteins and functional protein networks. J Biol Chem. 2013;288:13775-88 pubmed publisher
	Guillemot L, Schneider Y, Brun P, Castagliuolo I, Pizzuti D, Martines D, et al. Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury. J Cell Sci. 2012;125:5005-14 pubmed publisher
	Tabariès S, Dupuy F, Dong Z, Monast A, Annis M, Spicer J, et al. Claudin-2 promotes breast cancer liver metastasis by facilitating tumor cell interactions with hepatocytes. Mol Cell Biol. 2012;32:2979-91 pubmed publisher
	Fanning A, Van Itallie C, Anderson J. Zonula occludens-1 and -2 regulate apical cell structure and the zonula adherens cytoskeleton in polarized epithelia. Mol Biol Cell. 2012;23:577-90 pubmed publisher
	Collins J, Kirk A, Campbell S, Mason J, Wilson S. Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies. Methods Mol Biol. 2011;762:371-82 pubmed publisher
	Dhawan P, Ahmad R, Chaturvedi R, Smith J, Midha R, Mittal M, et al. Claudin-2 expression increases tumorigenicity of colon cancer cells: role of epidermal growth factor receptor activation. Oncogene. 2011;30:3234-47 pubmed publisher
	Kirk A, Campbell S, Bass P, Mason J, Collins J. Differential expression of claudin tight junction proteins in the human cortical nephron. Nephrol Dial Transplant. 2010;25:2107-19 pubmed publisher
	Wang J, Qin R, Ma Y, Wu H, Peters H, Tyska M, et al. Differential gene expression in normal esophagus and Barrett's esophagus. J Gastroenterol. 2009;44:897-911 pubmed publisher
	Coyne C, Gambling T, Boucher R, Carson J, Johnson L. Role of claudin interactions in airway tight junctional permeability. Am J Physiol Lung Cell Mol Physiol. 2003;285:L1166-78 pubmed
	Colegio O, Van Itallie C, Rahner C, Anderson J. Claudin extracellular domains determine paracellular charge selectivity and resistance but not tight junction fibril architecture. Am J Physiol Cell Physiol. 2003;284:C1346-54 pubmed

product information

Product Type :

Antibody

Product Name :

Claudin 2 Monoclonal Antibody (12H12)

Catalog # :

32-5600

Quantity :

100 µg

Price :

US 404.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Rat

Applications :

Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1-3 µg/mL

Species :

Canine, Human, Mouse, Rat

Clone :

12H12

Isotype :

IgG2b

Storage :

-20°C

Description :

Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. In freeze-fracture electron micrographs, tight junctions are visualized as belt-like bands of anastomosing sealing strands (TJ strands) that completely encircle the lateral surfaces of each cell. TJ strands on adjacent cells are presumed to interact with each other to form a sort of "molecular gasket" that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other. In addition to this so-called "barrier" function, the "fence" function of tight junctions plays an important role in maintaining epithelial cell-polarity by blocking the diffusion of membrane proteins between apical (luminal) and basolateral cell surfaces. Confinement of, for example, the glucose symport to apical surfaces allows glucose to be transported vectorially from the lumen, through the cell, and into the bloodstream. Several peripheral membrane proteins are associated with tight junctions including ZO-1, ZO-2, ZO-3 (members of membrane-associated guanylate-kinase family), cingulin, the 7H6 antigen, Rab-3b, symplekin. While their precise functions are not known, roles for these proteins have been suggested in tight junction assembly and maintenance; signal transduction; and the regulation of tight junction permeability. Furthermore, a growing body of evidence suggests that actin filaments play a major role in regulating tight junction permeability. Until recently, the only transmembrane protein known to be associated with tight junctions was occludin, an ~65 kDa protein with four transmembrane domains. Despite widespread expectation, a critical structural role for occludin in TJ strands was ruled out by the observation of apparently normal tight junctions formed between cells disrupted at both occludin alleles. Fortunately, a closer examination of isolated tight junctions uncovered two related ~22 kDa, four-transmembrane domain proteins, claudin-1 and claudin-2, with no similarity to occludin. In contrast to occludin, which induces only a small number of short strands at cell-cell contact sites when introduced into fibroblasts lacking tight junctions, claudin-1 and -2 induce networks of strands characteristic of true tight junctions. Though inconclusive, these findings suggest that claudin-1 and -2 are major structural components of TJ strands and that occludin plays some other accessory role. Excitement in the tight junction field continues to rise following the recent discovery of claudins -3, -4, -5, -6, -7, and -8 and experiments suggesting that tight junctions in different tissues are comprised of different sets of claudin family proteins. Claudin-1 and claudin-2 connect to the actin cytoskeleton through ZO-1; Claudin-2 functions as a paracellular channel with cation (Na+) selectivity at tight junctions. The expression of claudin-2 is restricted to the liver and kidney, with small amounts also found in the brain.

Immunogen :

Synthetic peptide corresponding to a 26 amino acid sequence at the C-terminus of mouse Claudin-2

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1-3 µg/mL

Aliases :

AL022813; claudin 2; Claudin2; claudin-2; CLD2; Cldn2; integral membrane protein claudin-2; PSEC0059; RGD1560247; SP82; UNQ705/PRO1356

more info or order :

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