product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
NPM1 Monoclonal Antibody (FC-61991)
catalog :
32-5200
quantity :
100 µg
price :
US 500.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
FC-61991
reactivity :
hamsters, human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, RNA immunoprecipitation
more info or order :
citations: 117
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; hamsters; 5 ug/ml; loading ...; fig 1c
Li X, Chen X, Gao J, Xian J, Li Z, Bi L, et al. Loss-of-function Mutations K11E or E271K Lead to Novel Tumor Suppression, Implicate Nucleolar Helicase DDX24 Oncogenicity. Int J Med Sci. 2022;19:596-608 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 3c
Mohan H, Trzeciakiewicz H, Pithadia A, Crowley E, Pacitto R, Safren N, et al. RTL8 promotes nuclear localization of UBQLN2 to subnuclear compartments associated with protein quality control. Cell Mol Life Sci. 2022;79:176 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 2a
Frottin F, Pérez Berlanga M, Hartl F, Hipp M. Multiple pathways of toxicity induced by C9orf72 dipeptide repeat aggregates and G4C2 RNA in a cellular model. elife. 2021;10: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 4d
Stadler D, Kächele M, Jones A, Hess J, Urban C, Schneider J, et al. Interferon-induced degradation of the persistent hepatitis B virus cccDNA form depends on ISG20. EMBO Rep. 2021;22:e49568 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 6a
Wang X, Zhang H, Sapio R, Yang J, Wong J, Zhang X, et al. SOD1 regulates ribosome biogenesis in KRAS mutant non-small cell lung cancer. Nat Commun. 2021;12:2259 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1a
  • western blot; human; loading ...; fig s3d
Frottin F, Schueder F, Tiwary S, Gupta R, Korner R, Schlichthaerle T, et al. The nucleolus functions as a phase-separated protein quality control compartment. Science. 2019;365:342-347 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 1d, 1s2g, 3s3c
Azkanaz M, Rodríguez López A, de Boer B, Huiting W, Angrand P, Vellenga E, et al. Protein quality control in the nucleolus safeguards recovery of epigenetic regulators after heat shock. elife. 2019;8: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s2e
López Erauskin J, Tadokoro T, Baughn M, Myers B, McAlonis Downes M, Chillon Marinas C, et al. ALS/FTD-Linked Mutation in FUS Suppresses Intra-axonal Protein Synthesis and Drives Disease Without Nuclear Loss-of-Function of FUS. Neuron. 2018;100:816-830.e7 pubmed publisher
  • western blot; human; loading ...; fig s5a
Marchesini M, Ogoti Y, Fiorini E, Aktaş Samur A, Nezi L, D Anca M, et al. ILF2 Is a Regulator of RNA Splicing and DNA Damage Response in 1q21-Amplified Multiple Myeloma. Cancer Cell. 2017;32:88-100.e6 pubmed publisher
  • immunoprecipitation; human; loading ...
  • immunocytochemistry; human
Ren Z, Aerts J, Vandenplas H, Wang J, Gorbenko O, Chen J, et al. Phosphorylated STAT5 regulates p53 expression via BRCA1/BARD1-NPM1 and MDM2. Cell Death Dis. 2016;7:e2560 pubmed publisher
  • western blot; human; loading ...
Hoch N, Hanzlikova H, Rulten S, Tetreault M, Komulainen E, Ju L, et al. XRCC1 mutation is associated with PARP1 hyperactivation and cerebellar ataxia. Nature. 2017;541:87-91 pubmed publisher
  • western blot; human; loading ...; fig 1a
Lin J, Hisaoka M, Nagata K, Okuwaki M. Functional characterization and efficient detection of Nucleophosmin/NPM1 oligomers. Biochem Biophys Res Commun. 2016;480:702-708 pubmed publisher
  • immunohistochemistry; human; loading ...; fig s5a
Hanzlikova H, Gittens W, Krejcikova K, Zeng Z, Caldecott K. Overlapping roles for PARP1 and PARP2 in the recruitment of endogenous XRCC1 and PNKP into oxidized chromatin. Nucleic Acids Res. 2017;45:2546-2557 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2b
  • western blot; human; loading ...; fig 3
Kozakai Y, Kamada R, Furuta J, Kiyota Y, Chuman Y, Sakaguchi K. PPM1D controls nucleolar formation by up-regulating phosphorylation of nucleophosmin. Sci Rep. 2016;6:33272 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3a
Okuwaki M, Abe M, Hisaoka M, Nagata K. Regulation of Cellular Dynamics and Chromosomal Binding Site Preference of Linker Histones H1.0 and H1.X. Mol Cell Biol. 2016;36:2681-2696 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 4
Li M, Liu D, Wang L, Wang W, Wang A, Yao Y. Expression of placenta-specific 8 in human oocytes, embryos, and models of in vitro implantation. Fertil Steril. 2016;106:781-789.e2 pubmed publisher
  • western blot; human; 1:5000; fig 5
Mukhopadhyay A, Sehgal L, Bose A, Gulvady A, Senapati P, Thorat R, et al. 14-3-3? Prevents Centrosome Amplification and Neoplastic Progression. Sci Rep. 2016;6:26580 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 3
Karlsson T, Altankhuyag A, Dobrovolska O, Turcu D, Lewis A. A polybasic motif in ErbB3-binding protein 1 (EBP1) has key functions in nucleolar localization and polyphosphoinositide interaction. Biochem J. 2016;473:2033-47 pubmed publisher
  • western blot; human; fig 3
Ceccon M, Merlo M, Mologni L, Poggio T, Varesio L, Menotti M, et al. Excess of NPM-ALK oncogenic signaling promotes cellular apoptosis and drug dependency. Oncogene. 2016;35:3854-3865 pubmed publisher
  • western blot; human
Neo S, Itahana Y, Alagu J, Kitagawa M, Guo A, Lee S, et al. TRIM28 Is an E3 Ligase for ARF-Mediated NPM1/B23 SUMOylation That Represses Centrosome Amplification. Mol Cell Biol. 2015;35:2851-63 pubmed publisher
  • immunocytochemistry; human
Sokka M, Rilla K, Miinalainen I, Pospiech H, Syväoja J. High levels of TopBP1 induce ATR-dependent shut-down of rRNA transcription and nucleolar segregation. Nucleic Acids Res. 2015;43:4975-89 pubmed publisher
  • western blot; human
Perera Y, Pedroso S, Borras Hidalgo O, Vázquez D, Miranda J, Villareal A, et al. Pharmacologic inhibition of the CK2-mediated phosphorylation of B23/NPM in cancer cells selectively modulates genes related to protein synthesis, energetic metabolism, and ribosomal biogenesis. Mol Cell Biochem. 2015;404:103-12 pubmed publisher
  • immunocytochemistry; human; 1:1000
Martelli M, Gionfriddo I, Mezzasoma F, Milano F, Pierangeli S, Mulas F, et al. Arsenic trioxide and all-trans retinoic acid target NPM1 mutant oncoprotein levels and induce apoptosis in NPM1-mutated AML cells. Blood. 2015;125:3455-65 pubmed publisher
  • immunocytochemistry; rat; 1:1000; fig 2
Holley C, Li M, Scruggs B, Matkovich S, Ory D, Schaffer J. Cytosolic accumulation of small nucleolar RNAs (snoRNAs) is dynamically regulated by NADPH oxidase. J Biol Chem. 2015;290:11741-8 pubmed publisher
  • western blot; human
Fujiwara Y, Hase K, Wada K, Kabuta T. An RNautophagy/DNautophagy receptor, LAMP2C, possesses an arginine-rich motif that mediates RNA/DNA-binding. Biochem Biophys Res Commun. 2015;460:281-6 pubmed publisher
  • western blot; human; fig 5
Katagiri N, Kuroda T, Kishimoto H, Hayashi Y, Kumazawa T, Kimura K. The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci Rep. 2015;5:8903 pubmed publisher
  • western blot; mouse; fig s2
Pekkonen P, Järviluoma A, Zinovkina N, Cvrljevic A, Prakash S, Westermarck J, et al. KSHV viral cyclin interferes with T-cell development and induces lymphoma through Cdk6 and Notch activation in vivo. Cell Cycle. 2014;13:3670-84 pubmed publisher
  • immunocytochemistry; mouse; fig s1
Lindenboim L, Sasson T, Worman H, Borner C, Stein R. Cellular stress induces Bax-regulated nuclear bubble budding and rupture followed by nuclear protein release. Nucleus. 2014;5:527-41 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Raman N, Nayak A, Muller S. mTOR signaling regulates nucleolar targeting of the SUMO-specific isopeptidase SENP3. Mol Cell Biol. 2014;34:4474-84 pubmed publisher
  • immunohistochemistry - paraffin section; human
  • immunocytochemistry; human
Peltonen K, Colis L, Liu H, Jäämaa S, Zhang Z, Af Hällström T, et al. Small molecule BMH-compounds that inhibit RNA polymerase I and cause nucleolar stress. Mol Cancer Ther. 2014;13:2537-46 pubmed publisher
  • immunocytochemistry; mouse; fig s9
Flach J, Bakker S, Mohrin M, Conroy P, Pietras E, Reynaud D, et al. Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells. Nature. 2014;512:198-202 pubmed publisher
  • immunocytochemistry; human; 1:200
Munne P, Gu Y, Tumiati M, Gao P, Koopal S, Uusivirta S, et al. TP53 supports basal-like differentiation of mammary epithelial cells by preventing translocation of deltaNp63 into nucleoli. Sci Rep. 2014;4:4663 pubmed publisher
  • western blot; human; 1:500; fig 1
Leal M, Mazzotti T, Calcagno D, Cirilo P, Martinez M, Demachki S, et al. Deregulated expression of Nucleophosmin 1 in gastric cancer and its clinicopathological implications. BMC Gastroenterol. 2014;14:9 pubmed publisher
  • western blot; human; fig 5
Lin Y, Chen Y, Cohen S, Cheng T. Identification of TSG101 functional domains and p21 loci required for TSG101-mediated p21 gene regulation. PLoS ONE. 2013;8:e79674 pubmed publisher
  • western blot; human; fig 2
de Poot S, Lai K, van der Wal L, Plasman K, Van Damme P, Porter A, et al. Granzyme M targets topoisomerase II alpha to trigger cell cycle arrest and caspase-dependent apoptosis. Cell Death Differ. 2014;21:416-26 pubmed publisher
  • western blot; human
Harrison Pitner M, Saavedra H. Cdk4 and nek2 signal binucleation and centrosome amplification in a her2+ breast cancer model. PLoS ONE. 2013;8:e65971 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 2
Foroni L, Vasuri F, Valente S, Gualandi C, Focarete M, Caprara G, et al. The role of 3D microenvironmental organization in MCF-7 epithelial-mesenchymal transition after 7 culture days. Exp Cell Res. 2013;319:1515-22 pubmed publisher
  • western blot; human; fig 1
Noguera N, Song M, Divona M, Catalano G, Calvo K, Garcia F, et al. Nucleophosmin/B26 regulates PTEN through interaction with HAUSP in acute myeloid leukemia. Leukemia. 2013;27:1037-43 pubmed publisher
  • western blot; human; 1:1000; fig 4
Fukawa T, Ono M, Matsuo T, Uehara H, Miki T, Nakamura Y, et al. DDX31 regulates the p53-HDM2 pathway and rRNA gene transcription through its interaction with NPM1 in renal cell carcinomas. Cancer Res. 2012;72:5867-77 pubmed publisher
  • western blot; human; fig 12
Lin H, Lin Y, Hsu F. Preparation and characterization of mesoporous bioactive glass/polycaprolactone nanofibrous matrix for bone tissues engineering. J Mater Sci Mater Med. 2012;23:2619-30 pubmed publisher
  • immunocytochemistry; mouse; fig 5
Mercier I, Camacho J, Titchen K, Gonzales D, Quann K, Bryant K, et al. Caveolin-1 and accelerated host aging in the breast tumor microenvironment: chemoprevention with rapamycin, an mTOR inhibitor and anti-aging drug. Am J Pathol. 2012;181:278-93 pubmed publisher
  • immunocytochemistry; mouse; 1 ug/ml; fig S7
Li Z, Hann S. Nucleophosmin is essential for c-Myc nucleolar localization and c-Myc-mediated rDNA transcription. Oncogene. 2013;32:1988-94 pubmed publisher
  • immunocytochemistry; human; 500 ug/ml
Weston D, Adams N, Russell R, Stephens D, Freemont P. Analysis of spatial point patterns in nuclear biology. PLoS ONE. 2012;7:e36841 pubmed publisher
  • western blot; human; 1 ug/ml; fig 3
Perera Y, Costales H, Diaz Y, Reyes O, Farina H, Mendez L, et al. Sensitivity of tumor cells towards CIGB-300 anticancer peptide relies on its nucleolar localization. J Pept Sci. 2012;18:215-23 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 1
Sari A, Calli A, Altinboga A, Pehlivan F, Görgel S, Balci U, et al. Nucleophosmin expression in renal cell carcinoma and oncocytoma. APMIS. 2012;120:187-94 pubmed publisher
  • western blot; human; fig s1
Tsai S, Liou H, Lin C, Kuo K, Hung Y, Weng R, et al. MG63 osteoblast-like cells exhibit different behavior when grown on electrospun collagen matrix versus electrospun gelatin matrix. PLoS ONE. 2012;7:e31200 pubmed publisher
  • immunocytochemistry; mouse; fig 1
  • western blot; mouse; fig 6
Saporita A, Chang H, Winkeler C, Apicelli A, Kladney R, Wang J, et al. RNA helicase DDX5 is a p53-independent target of ARF that participates in ribosome biogenesis. Cancer Res. 2011;71:6708-17 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 2
Calli A, Sari A, Evcim G, Altinboga A, Sadullahoglu C, Ermete M. The enigmatic role of nucleophosmin in malignant melanoma: does it have an effect?. Indian J Pathol Microbiol. 2011;54:482-6 pubmed publisher
  • western blot; human; fig 5
de Koning P, Kummer J, de Poot S, Quadir R, Broekhuizen R, McGettrick A, et al. Intracellular serine protease inhibitor SERPINB4 inhibits granzyme M-induced cell death. PLoS ONE. 2011;6:e22645 pubmed publisher
  • immunocytochemistry; human; fig 1
Østrup O, Hyttel P, Klærke D, Collas P. Remodeling of ribosomal genes in somatic cells by Xenopus egg extract. Biochem Biophys Res Commun. 2011;412:487-93 pubmed publisher
  • immunocytochemistry; human; fig 3
Teittinen K, Kärkkäinen P, Salonen J, Rönnholm G, Korkeamäki H, Vihinen M, et al. Nucleolar proteins with altered expression in leukemic cell lines. Leuk Res. 2012;36:232-6 pubmed publisher
  • immunocytochemistry; human; fig 1
Donati G, Bertoni S, Brighenti E, Vici M, Trere D, Volarevic S, et al. The balance between rRNA and ribosomal protein synthesis up- and downregulates the tumour suppressor p53 in mammalian cells. Oncogene. 2011;30:3274-88 pubmed publisher
  • immunocytochemistry; human; fig 3
Finkbeiner E, Haindl M, Muller S. The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex. EMBO J. 2011;30:1067-78 pubmed publisher
  • western blot; human; fig 4
Welcker M, Larimore E, Frappier L, Clurman B. Nucleolar targeting of the fbw7 ubiquitin ligase by a pseudosubstrate and glycogen synthase kinase 3. Mol Cell Biol. 2011;31:1214-24 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 3
Dutt S, Narla A, Lin K, Mullally A, Abayasekara N, Megerdichian C, et al. Haploinsufficiency for ribosomal protein genes causes selective activation of p53 in human erythroid progenitor cells. Blood. 2011;117:2567-76 pubmed publisher
  • western blot; human; fig 1
Lewis A, Sommer L, Arntzen M, Strahm Y, Morrice N, Divecha N, et al. Identification of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins by neomycin extraction. Mol Cell Proteomics. 2011;10:M110.003376 pubmed publisher
  • immunoprecipitation; human; fig 5
Fantini D, Vascotto C, Marasco D, D Ambrosio C, Romanello M, Vitagliano L, et al. Critical lysine residues within the overlooked N-terminal domain of human APE1 regulate its biological functions. Nucleic Acids Res. 2010;38:8239-56 pubmed publisher
  • immunocytochemistry; human; fig 2
Zhu Z, Wang Y, Li X, Wang Y, Xu L, Wang X, et al. PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis. Cell Res. 2010;20:794-801 pubmed publisher
  • western blot; mouse; fig 4
Kawagishi H, Nakamura H, Maruyama M, Mizutani S, Sugimoto K, Takagi M, et al. ARF suppresses tumor angiogenesis through translational control of VEGFA mRNA. Cancer Res. 2010;70:4749-58 pubmed publisher
  • immunocytochemistry; human; 1 ug/ml; fig 4
Rees Unwin K, Faragher R, Unwin R, Adams J, Brown P, Buckle A, et al. Ribosome-associated nucleophosmin 1: increased expression and shuttling activity distinguishes prognostic subtypes in chronic lymphocytic leukaemia. Br J Haematol. 2010;148:534-43 pubmed publisher
  • immunohistochemistry; human
Pitulescu M, Teichmann M, Luo L, Kessel M. TIPT2 and geminin interact with basal transcription factors to synergize in transcriptional regulation. BMC Biochem. 2009;10:16 pubmed publisher
  • western blot; human
Duxin J, Dao B, Martinsson P, Rajala N, Guittat L, Campbell J, et al. Human Dna2 is a nuclear and mitochondrial DNA maintenance protein. Mol Cell Biol. 2009;29:4274-82 pubmed publisher
  • western blot; human; 1 ug/ml; fig 2
Di Tommaso A, Hagen J, Tompkins V, Muniz V, Dudakovic A, Kitzis A, et al. Residues in the alternative reading frame tumor suppressor that influence its stability and p53-independent activities. Exp Cell Res. 2009;315:1326-35 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Vascotto C, Fantini D, Romanello M, Cesaratto L, Deganuto M, Leonardi A, et al. APE1/Ref-1 interacts with NPM1 within nucleoli and plays a role in the rRNA quality control process. Mol Cell Biol. 2009;29:1834-54 pubmed publisher
  • immunocytochemistry; mouse; fig 3
Zhang J, Tomasini A, Mayer A. RBM19 is essential for preimplantation development in the mouse. BMC Dev Biol. 2008;8:115 pubmed publisher
  • western blot; human; fig 4
Itahana K, Zhang Y. Mitochondrial p32 is a critical mediator of ARF-induced apoptosis. Cancer Cell. 2008;13:542-53 pubmed publisher
  • immunoprecipitation; mouse; fig 5
  • immunocytochemistry; mouse; fig 1
  • western blot; mouse; fig 1
Yogev O, Saadon K, Anzi S, Inoue K, Shaulian E. DNA damage-dependent translocation of B23 and p19 ARF is regulated by the Jun N-terminal kinase pathway. Cancer Res. 2008;68:1398-406 pubmed publisher
  • western blot; mouse; fig 5
Apicelli A, Maggi L, Hirbe A, Miceli A, Olanich M, Schulte Winkeler C, et al. A non-tumor suppressor role for basal p19ARF in maintaining nucleolar structure and function. Mol Cell Biol. 2008;28:1068-80 pubmed
  • immunocytochemistry; human; fig 4
Korkeamäki H, Viiri K, Kukkonen M, Maki M, Lohi O. Alternative mRNA splicing of SAP30L regulates its transcriptional repression activity. FEBS Lett. 2008;582:379-84 pubmed
  • immunoprecipitation; human; fig 1
  • western blot; human
Frescas D, Guardavaccaro D, Bassermann F, Koyama Nasu R, Pagano M. JHDM1B/FBXL10 is a nucleolar protein that represses transcription of ribosomal RNA genes. Nature. 2007;450:309-13 pubmed
  • western blot; human
Moulin S, Llanos S, Kim S, Peters G. Binding to nucleophosmin determines the localization of human and chicken ARF but not its impact on p53. Oncogene. 2008;27:2382-9 pubmed
  • western blot; rat; fig 3
Gerashchenko B, Yamagata A, Oofusa K, Yoshizato K, De Toledo S, Howell R. Proteome analysis of proliferative response of bystander cells adjacent to cells exposed to ionizing radiation. Proteomics. 2007;7:2000-8 pubmed
  • western blot; human
Bergstralh D, Conti B, Moore C, Brickey W, Taxman D, Ting J. Global functional analysis of nucleophosmin in Taxol response, cancer, chromatin regulation, and ribosomal DNA transcription. Exp Cell Res. 2007;313:65-76 pubmed
  • immunocytochemistry; human
Viiri K, Korkeamäki H, Kukkonen M, Nieminen L, Lindfors K, Peterson P, et al. SAP30L interacts with members of the Sin3A corepressor complex and targets Sin3A to the nucleolus. Nucleic Acids Res. 2006;34:3288-98 pubmed
  • immunohistochemistry; human; fig 1
Falini B, Martelli M, Bolli N, Bonasso R, Ghia E, Pallotta M, et al. Immunohistochemistry predicts nucleophosmin (NPM) mutations in acute myeloid leukemia. Blood. 2006;108:1999-2005 pubmed
  • western blot; human; fig 6
Kinumi T, Ogawa Y, Kimata J, Saito Y, Yoshida Y, Niki E. Proteomic characterization of oxidative dysfunction in human umbilical vein endothelial cells (HUVEC) induced by exposure to oxidized LDL. Free Radic Res. 2005;39:1335-44 pubmed
  • immunocytochemistry; human; fig 4A
  • western blot; human; fig 4B
Wang W, Budhu A, Forgues M, Wang X. Temporal and spatial control of nucleophosmin by the Ran-Crm1 complex in centrosome duplication. Nat Cell Biol. 2005;7:823-30 pubmed
Holsapple J, Schnitzler L, Rusch L, Baldeweg T, Neubert E, Kruss S, et al. Expansion microscopy of neutrophil nuclear structure and extracellular traps. Biophys Rep (N Y). 2023;3:100091 pubmed publisher
Chen K, Li W, Xu K. Super-multiplexing excitation spectral microscopy with multiple fluorescence bands. Biomed Opt Express. 2022;13:6048-6060 pubmed publisher
xd6 z xe7 elik E, Kalayc x131 A, xc7 elik B, Avc x131 A, Akyol H, K x131 l x131 xe7 x, et al. Doxorubicin induces prolonged DNA damage signal in cells overexpressing DEK isoform-2. PLoS ONE. 2022;17:e0275476 pubmed publisher
Jing Y, Jiang X, Lei L, Peng M, Ren J, Xiao Q, et al. Mutant NPM1-regulated lncRNA HOTAIRM1 promotes leukemia cell autophagy and proliferation by targeting EGR1 and ULK3. J Exp Clin Cancer Res. 2021;40:312 pubmed publisher
Sawazaki H, Ito K, Asano T, Kuroda K, Horiguchi A, Tsuda H, et al. Expressions of P-Glycoprotein, Multidrug Resistance Protein 1 and Annexin A2 as Predictive Factors for Intravesical Recurrence of Bladder Cancer after the Initial Transurethral Resection and Immediate Single Intravesical Instillation of Adriamycin. Asian Pac J Cancer Prev. 2021;22:1459-1466 pubmed publisher
Sapio R, Burns C, Pestov D. Effects of Hydrogen Peroxide Stress on the Nucleolar Redox Environment and Pre-rRNA Maturation. Front Mol Biosci. 2021;8:678488 pubmed publisher
Chen K, Yan R, Xiang L, Xu K. Excitation spectral microscopy for highly multiplexed fluorescence imaging and quantitative biosensing. Light Sci Appl. 2021;10:97 pubmed publisher
Bose A, Modi K, Dey S, Dalvi S, Nadkarni P, Sudarshan M, et al. 14-3-3γ prevents centrosome duplication by inhibiting NPM1 function. Genes Cells. 2021;26:426-446 pubmed publisher
Bhattacharyya S, Oon C, Kothari A, Horton W, Link J, Sears R, et al. Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer. J Exp Med. 2020;217: pubmed publisher
Xie L, Rajpurkar A, Quarles E, Taube N, Rai A, Erba J, et al. Accumulation of Nucleolar Inorganic Polyphosphate Is a Cellular Response to Cisplatin-Induced Apoptosis. Front Oncol. 2019;9:1410 pubmed publisher
Burra S, Marasco D, Malfatti M, Antoniali G, Virgilio A, Esposito V, et al. Human AP-endonuclease (Ape1) activity on telomeric G4 structures is modulated by acetylatable lysine residues in the N-terminal sequence. DNA Repair (Amst). 2019;73:129-143 pubmed publisher
Hayashi Y, Fujimura A, Kato K, Udagawa R, Hirota T, Kimura K. Nucleolar integrity during interphase supports faithful Cdk1 activation and mitotic entry. Sci Adv. 2018;4:eaap7777 pubmed publisher
Hensen F, Moretton A, van Esveld S, Farge G, Spelbrink J. The mitochondrial outer-membrane location of the EXD2 exonuclease contradicts its direct role in nuclear DNA repair. Sci Rep. 2018;8:5368 pubmed publisher
Schueder F, Lara Gutiérrez J, Beliveau B, Saka S, Sasaki H, Woehrstein J, et al. Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT. Nat Commun. 2017;8:2090 pubmed publisher
Freudenberger N, Meyer T, Groitl P, Dobner T, Schreiner S. Human Adenovirus Core Protein V Is Targeted by the Host SUMOylation Machinery To Limit Essential Viral Functions. J Virol. 2018;92: pubmed publisher
Li M, Sletten A, Lee J, Pyles K, Matkovich S, Ory D, et al. Nuclear export factor 3 regulates localization of small nucleolar RNAs. J Biol Chem. 2017;292:20228-20239 pubmed publisher
Ando K, PARSONS M, Shah R, Charendoff C, Paris S, Liu P, et al. NPM1 directs PIDDosome-dependent caspase-2 activation in the nucleolus. J Cell Biol. 2017;216:1795-1810 pubmed publisher
Shumilov A, Tsai M, Schlosser Y, Kratz A, Bernhardt K, Fink S, et al. Epstein-Barr virus particles induce centrosome amplification and chromosomal instability. Nat Commun. 2017;8:14257 pubmed publisher
Andresen V, Erikstein B, Mukherjee H, Sulen A, Popa M, S rnes S, et al. Anti-proliferative activity of the NPM1 interacting natural product avrainvillamide in acute myeloid leukemia. Cell Death Dis. 2016;7:e2497 pubmed publisher
Meng X, Tackmann N, Liu S, Yang J, Dong J, Wu C, et al. RPL23 Links Oncogenic RAS Signaling to p53-Mediated Tumor Suppression. Cancer Res. 2016;76:5030-9 pubmed publisher
Day P, Thompson C, Pang Y, Lowy D, Schiller J. Involvement of Nucleophosmin (NPM1/B23) in Assembly of Infectious HPV16 Capsids. Papillomavirus Res. 2015;1:74-89 pubmed
Naylor R, Jeganathan K, Cao X, van Deursen J. Nuclear pore protein NUP88 activates anaphase-promoting complex to promote aneuploidy. J Clin Invest. 2016;126:543-59 pubmed publisher
Reed S, Hagen J, Tompkins V, Thies K, Quelle F, Quelle D. Nuclear interactor of ARF and Mdm2 regulates multiple pathways to activate p53. Cell Cycle. 2014;13:1288-98 pubmed publisher
Xia X, Liu S, Xiao Z, Zhu F, Song N, Zhou M, et al. An IKKα-nucleophosmin axis utilizes inflammatory signaling to promote genome integrity. Cell Rep. 2013;5:1243-55 pubmed publisher
Li T, Lu Q, Wang G, Xu H, Huang H, Cai T, et al. SET-domain bacterial effectors target heterochromatin protein 1 to activate host rDNA transcription. EMBO Rep. 2013;14:733-40 pubmed publisher
Bai B, Moore H, Laiho M. CRM1 and its ribosome export adaptor NMD3 localize to the nucleolus and affect rRNA synthesis. Nucleus. 2013;4:315-25 pubmed publisher
Pichugin A, Beaujean N, Vignon X, Vassetzky Y. Ring-like distribution of constitutive heterochromatin in bovine senescent cells. PLoS ONE. 2011;6:e26844 pubmed publisher
Gakovic M, Shu X, Kasioulis I, Carpanini S, Moraga I, Wright A. The role of RPGR in cilia formation and actin stability. Hum Mol Genet. 2011;20:4840-50 pubmed publisher
Piazza F, Manni S, Tubi L, Montini B, Pavan L, Colpo A, et al. Glycogen Synthase Kinase-3 regulates multiple myeloma cell growth and bortezomib-induced cell death. BMC Cancer. 2010;10:526 pubmed publisher
Wang Z, Udeshi N, Slawson C, Compton P, Sakabe K, Cheung W, et al. Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis. Sci Signal. 2010;3:ra2 pubmed publisher
Zhang J, Yang Y, Wu J. B23 interacts with PES1 and is involved in nucleolar localization of PES1. Acta Biochim Biophys Sin (Shanghai). 2009;41:991-7 pubmed
Russell R, Adams N, Stephens D, Batty E, Jensen K, Freemont P. Segmentation of fluorescence microscopy images for quantitative analysis of cell nuclear architecture. Biophys J. 2009;96:3379-89 pubmed publisher
Bevington J, Needham P, Verrill K, Collaco R, Basrur V, Trempe J. Adeno-associated virus interactions with B23/Nucleophosmin: identification of sub-nucleolar virion regions. Virology. 2007;357:102-13 pubmed
Kalin T, Wang I, Ackerson T, Major M, Detrisac C, Kalinichenko V, et al. Increased levels of the FoxM1 transcription factor accelerate development and progression of prostate carcinomas in both TRAMP and LADY transgenic mice. Cancer Res. 2006;66:1712-20 pubmed
Mai R, Yeh T, Kao C, Sun S, Huang H, Wu Lee Y. Hepatitis C virus core protein recruits nucleolar phosphoprotein B23 and coactivator p300 to relieve the repression effect of transcriptional factor YY1 on B23 gene expression. Oncogene. 2006;25:448-62 pubmed publisher
Tago K, Chiocca S, Sherr C. Sumoylation induced by the Arf tumor suppressor: a p53-independent function. Proc Natl Acad Sci U S A. 2005;102:7689-94 pubmed
Li Y, Schwabe R, DeVries Seimon T, Yao P, Gerbod Giannone M, Tall A, et al. Free cholesterol-loaded macrophages are an abundant source of tumor necrosis factor-alpha and interleukin-6: model of NF-kappaB- and map kinase-dependent inflammation in advanced atherosclerosis. J Biol Chem. 2005;280:21763-72 pubmed
Dasgupta B, Yi Y, Chen D, Weber J, Gutmann D. Proteomic analysis reveals hyperactivation of the mammalian target of rapamycin pathway in neurofibromatosis 1-associated human and mouse brain tumors. Cancer Res. 2005;65:2755-60 pubmed
Korgaonkar C, Hagen J, Tompkins V, Frazier A, Allamargot C, Quelle F, et al. Nucleophosmin (B23) targets ARF to nucleoli and inhibits its function. Mol Cell Biol. 2005;25:1258-71 pubmed
Zhao L, Samuels T, Winckler S, Korgaonkar C, Tompkins V, Horne M, et al. Cyclin G1 has growth inhibitory activity linked to the ARF-Mdm2-p53 and pRb tumor suppressor pathways. Mol Cancer Res. 2003;1:195-206 pubmed
product information
Product Type :
Antibody
Product Name :
NPM1 Monoclonal Antibody (FC-61991)
Catalog # :
32-5200
Quantity :
100 µg
Price :
US 500.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Mouse, Rat
Applications :
ELISA: 1-2 µg/mL, Immunocytochemistry: 2-10 µg/mL, Immunohistochemistry: Assay-dependent, Immunoprecipitation: 10 µg, RNA Immunoprecipitation: Assay-dependent, Western Blot: 1-3 µg/mL
Species :
Human, Mouse, Rat
Clone :
FC-61991
Isotype :
IgG1
Storage :
-20°C
Description :
NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.
Immunogen :
NPM/B23 purified from rat hepatoma.
Format :
Liquid
Applications w/Dilutions :
ELISA: 1-2 µg/mL, Immunocytochemistry: 2-10 µg/mL, Immunohistochemistry: Assay-dependent, Immunoprecipitation: 10 µg, RNA Immunoprecipitation: Assay-dependent, Western Blot: 1-3 µg/mL
Aliases :
B23; B23 antibody; B23NP; MGC104254; mNPM antibody; mNPM1 antibody; NO38; NO38 antibody; Npm; npm1; npm1.L; Nucleolar phosphoprotein B23; nucleolar protein B23.1; nucleolar protein B23/No38; nucleolar protein NO38; Nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin (nucleolar phosphoprotein B23, numatrin) L homeolog; nucleophosmin 1; nucleophosmin/nucleoplasmin family, member 1; nucleoplasmin; numatrin; numatrin antibody; testicular tissue protein Li 128; unnamed protein product; XELAEV_18020022mg
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA