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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
SKP2 Monoclonal Antibody (SKP2-8D9)
catalog :
32-3300
quantity :
100 µg
price :
425 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SKP2-8D9
reactivity :
human, mouse
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 43
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 5e
von Gamm M, Schaub A, Jones A, Wolf C, Behrens G, Lichti J, et al. Immune homeostasis and regulation of the interferon pathway require myeloid-derived Regnase-3. J Exp Med. 2019;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 8a
Mentrup T, Theodorou K, Cabrera Cabrera F, Helbig A, Happ K, Gijbels M, et al. Atherogenic LOX-1 signaling is controlled by SPPL2-mediated intramembrane proteolysis. J Exp Med. 2019;: pubmed publisher
  • western blot; human; 1:1000; fig 1
Pavlides S, Lecanda J, Daubriac J, Pandya U, Gama P, Blank S, et al. TGF-β activates APC through Cdh1 binding for Cks1 and Skp2 proteasomal destruction stabilizing p27kip1 for normal endometrial growth. Cell Cycle. 2016;15:931-47 pubmed publisher
  • western blot; human; 1:1000
Evans L, Chen L, Milazzo G, Gherardi S, Perini G, Willmore E, et al. SKP2 is a direct transcriptional target of MYCN and a potential therapeutic target in neuroblastoma. Cancer Lett. 2015;363:37-45 pubmed publisher
  • western blot; human; 1:2000
Xu D, Li C, Zhang X, Gong Z, Chan C, Lee S, et al. Skp2-macroH2A1-CDK8 axis orchestrates G2/M transition and tumorigenesis. Nat Commun. 2015;6:6641 pubmed publisher
  • western blot; mouse
Chen J, Shin J, Zhao R, Phan L, Wang H, Xue Y, et al. CSN6 drives carcinogenesis by positively regulating Myc stability. Nat Commun. 2014;5:5384 pubmed publisher
  • western blot; human; 1:500-1:1000
Lupino E, Ramondetti C, Buccinnà B, Piccinini M. Exposure of neuroblastoma cell lines to imatinib results in the upregulation of the CDK inhibitor p27(KIP1) as a consequence of c-Abl inhibition. Biochem Pharmacol. 2014;92:235-50 pubmed publisher
  • western blot; human
Ram R, Mendiratta S, Bodemann B, Torres M, Eskiocak U, White M. RASSF1A inactivation unleashes a tumor suppressor/oncogene cascade with context-dependent consequences on cell cycle progression. Mol Cell Biol. 2014;34:2350-8 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
  • western blot; human; 1:1000; fig 7
Kim J, Kim H, Park J, Park D, Cho Y, Sohn C, et al. Epidermal growth factor upregulates Skp2/Cks1 and p27(kip1) in human extrahepatic cholangiocarcinoma cells. World J Gastroenterol. 2014;20:755-73 pubmed publisher
  • western blot; human; fig 6
Segura M, Fontanals Cirera B, Gaziel Sovran A, Guijarro M, Hanniford D, Zhang G, et al. BRD4 sustains melanoma proliferation and represents a new target for epigenetic therapy. Cancer Res. 2013;73:6264-76 pubmed publisher
  • western blot; human
Correa R, Peart T, Valdes Y, DiMattia G, Shepherd T. Modulation of AKT activity is associated with reversible dormancy in ascites-derived epithelial ovarian cancer spheroids. Carcinogenesis. 2012;33:49-58 pubmed publisher
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 2
Roe J, Kim H, Hwang I, Cho E, Youn H. von Hippel-Lindau protein promotes Skp2 destabilization on DNA damage. Oncogene. 2011;30:3127-38 pubmed publisher
  • immunocytochemistry; human; fig 1c
  • western blot; human; 1:2000; fig 1a
Rico Bautista E, Yang C, Lu L, Roth G, Wolf D. Chemical genetics approach to restoring p27Kip1 reveals novel compounds with antiproliferative activity in prostate cancer cells. BMC Biol. 2010;8:153 pubmed publisher
  • western blot; human; fig 8
Lupino E, Buccinnà B, Ramondetti C, Lomartire A, De Marco G, Ricotti E, et al. In CD28-costimulated human naïve CD4+ T cells, I-?B kinase controls the expression of cell cycle regulatory proteins via interleukin-2-independent mechanisms. Immunology. 2010;131:231-41 pubmed publisher
  • western blot; human; fig 2
Fernandez Sanchez M, Sechet E, Margottin Goguet F, Rogge L, Bianchi E. The human COP9 signalosome protects ubiquitin-conjugating enzyme 3 (UBC3/Cdc34) from beta-transducin repeat-containing protein (betaTrCP)-mediated degradation. J Biol Chem. 2010;285:17390-7 pubmed publisher
  • western blot; human; fig 3
Bellanger S, Tan C, Nei W, He P, Thierry F. The human papillomavirus type 18 E2 protein is a cell cycle-dependent target of the SCFSkp2 ubiquitin ligase. J Virol. 2010;84:437-44 pubmed publisher
  • western blot; human; fig 3
Jiang X, Austin P, Niederhoff R, Manson S, Riehm J, Cook B, et al. Mechanoregulation of proliferation. Mol Cell Biol. 2009;29:5104-14 pubmed publisher
  • western blot; human; fig 3
Hu R, Aplin A. Skp2 regulates G2/M progression in a p53-dependent manner. Mol Biol Cell. 2008;19:4602-10 pubmed publisher
  • flow cytometry; human; fig 1
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 1
Ji P, Sun D, Wang H, Bauzon F, Zhu L. Disrupting Skp2-cyclin A interaction with a blocking peptide induces selective cancer cell killing. Mol Cancer Ther. 2007;6:684-91 pubmed
  • western blot; human
Denti S, Fernandez Sanchez M, Rogge L, Bianchi E. The COP9 signalosome regulates Skp2 levels and proliferation of human cells. J Biol Chem. 2006;281:32188-96 pubmed
  • western blot; human; fig 5
Bhatt K, Hu R, Spofford L, Aplin A. Mutant B-RAF signaling and cyclin D1 regulate Cks1/S-phase kinase-associated protein 2-mediated degradation of p27Kip1 in human melanoma cells. Oncogene. 2007;26:1056-66 pubmed
  • western blot; human; fig 2
Ji P, Goldin L, Ren H, Sun D, Guardavaccaro D, Pagano M, et al. Skp2 contains a novel cyclin A binding domain that directly protects cyclin A from inhibition by p27Kip1. J Biol Chem. 2006;281:24058-69 pubmed
  • immunohistochemistry - paraffin section; human; 1:100; tbl 1
Langner C, Ratschek M, Rehak P, Tsybrovskyy O, Zigeuner R. The pT1a and pT1b category subdivision in renal cell carcinoma: is it reflected by differences in tumour biology?. BJU Int. 2005;95:310-4 pubmed
  • western blot; human
Lee S, McCormick F. Downregulation of Skp2 and p27/Kip1 synergistically induces apoptosis in T98G glioblastoma cells. J Mol Med (Berl). 2005;83:296-307 pubmed
  • western blot; human
Mammoto A, Huang S, Moore K, Oh P, Ingber D. Role of RhoA, mDia, and ROCK in cell shape-dependent control of the Skp2-p27kip1 pathway and the G1/S transition. J Biol Chem. 2004;279:26323-30 pubmed
  • western blot; human; fig 2
Liu E, Li X, Yan F, Zhao Q, Wu X. Cyclin-dependent kinases phosphorylate human Cdt1 and induce its degradation. J Biol Chem. 2004;279:17283-8 pubmed
  • western blot; human
Li X, Zhao Q, Liao R, Sun P, Wu X. The SCF(Skp2) ubiquitin ligase complex interacts with the human replication licensing factor Cdt1 and regulates Cdt1 degradation. J Biol Chem. 2003;278:30854-8 pubmed
  • western blot; human; 1:250
Lu L, Schulz H, Wolf D. The F-box protein SKP2 mediates androgen control of p27 stability in LNCaP human prostate cancer cells. BMC Cell Biol. 2002;3:22 pubmed
Li X, Yang S, Zhang M, Xie S, Xie Z. Downregulation of SRPK2 promotes cell cycle arrest though E2F1 in non-small cell lung cancer. Eur J Histochem. 2019;63: pubmed publisher
Bufalieri F, Infante P, Bernardi F, Caimano M, Romania P, Moretti M, et al. ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP. Nat Commun. 2019;10:3304 pubmed publisher
Zhang Y, Zvi Y, Batko B, Zaphiros N, O Donnell E, Wang J, et al. Down-regulation of Skp2 expression inhibits invasion and lung metastasis in osteosarcoma. Sci Rep. 2018;8:14294 pubmed publisher
Yao F, Zhou Z, Kim J, Hang Q, Xiao Z, Ton B, et al. SKP2- and OTUD1-regulated non-proteolytic ubiquitination of YAP promotes YAP nuclear localization and activity. Nat Commun. 2018;9:2269 pubmed publisher
Clément E, Inuzuka H, Nihira N, Wei W, Toker A. Skp2-dependent reactivation of AKT drives resistance to PI3K inhibitors. Sci Signal. 2018;11: pubmed publisher
Santos Barriopedro I, Bosch Presegué L, Marazuela Duque A, de la Torre C, Colomer C, Vazquez B, et al. SIRT6-dependent cysteine monoubiquitination in the PRE-SET domain of Suv39h1 regulates the NF-κB pathway. Nat Commun. 2018;9:101 pubmed publisher
Peng Y, Wu W, Chen L, Kuo K, Tsai C, Huang Y, et al. Upregulation of cyclin-dependent kinase inhibitors CDKN1B and CDKN1C in hepatocellular carcinoma-derived cells via goniothalamin-mediated protein stabilization and epigenetic modifications. Toxicol Rep. 2015;2:322-332 pubmed publisher
Jang W, Kim T, Koo J, Kim S, Lim D. Mechanical cue-induced YAP instructs Skp2-dependent cell cycle exit and oncogenic signaling. EMBO J. 2017;36:2510-2528 pubmed publisher
Yuan X, Srividhya J, De Luca T, Lee J, Pomerening J. Uncovering the role of APC-Cdh1 in generating the dynamics of S-phase onset. Mol Biol Cell. 2014;25:441-56 pubmed publisher
Sohn C, Agnew H, Lee J, Sweredoski M, Graham R, Smith G, et al. Designer reagents for mass spectrometry-based proteomics: clickable cross-linkers for elucidation of protein structures and interactions. Anal Chem. 2012;84:2662-9 pubmed publisher
Rose A, Wang G, Hanniford D, Monni S, Tu T, Shapiro R, et al. Clinical relevance of SKP2 alterations in metastatic melanoma. Pigment Cell Melanoma Res. 2011;24:197-206 pubmed publisher
Chen Q, Xie W, Kuhn D, Voorhees P, Lopez Girona A, Mendy D, et al. Targeting the p27 E3 ligase SCF(Skp2) results in p27- and Skp2-mediated cell-cycle arrest and activation of autophagy. Blood. 2008;111:4690-9 pubmed publisher
Rosner M, Hengstschlager M. Tuberin binds p27 and negatively regulates its interaction with the SCF component Skp2. J Biol Chem. 2004;279:48707-15 pubmed
Liang M, Liang Y, Wrighton K, Ungermannova D, Wang X, Brunicardi F, et al. Ubiquitination and proteolysis of cancer-derived Smad4 mutants by SCFSkp2. Mol Cell Biol. 2004;24:7524-37 pubmed
Chiarle R, Fan Y, Piva R, Boggino H, Skolnik J, Novero D, et al. S-phase kinase-associated protein 2 expression in non-Hodgkin's lymphoma inversely correlates with p27 expression and defines cells in S phase. Am J Pathol. 2002;160:1457-66 pubmed
product information
Product Type :
Antibody
Product Name :
SKP2 Monoclonal Antibody (SKP2-8D9)
Catalog # :
32-3300
Quantity :
100 µg
Price :
425 USD
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Non-human primate
Applications :
ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 1:250, Immunofluorescence: 1:250, Immunoprecipitation: 5 µg, Western Blot: 2 µg/mL
Species :
Human, Non-human primate
Clone :
SKP2-8D9
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
Immunogen :
Recombinant, full-length human p45SKP2 protein.
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 1:250, Immunofluorescence: 1:250, Immunoprecipitation: 5 µg, Western Blot: 2 µg/mL
Aliases :
4930500A04Rik; CDK2/cyclin A-associated protein p45; cyclin A/CDK2-associated protein p45; Cyclin-A/CDK2-associated protein p45; FBL1; F-box protein Skp2; F-box/LRR-repeat protein 1; F-box/WD-40 protein 1; FBXL1; FLB1; FWD1; MGC1366; OTTHUMP00000221389; p45; p45skp2; RGD1562456; Skp2; S-phase kinase-associated protein 2; S-phase kinase-associated protein 2 (p45); S-phase kinase-associated protein 2, E3 ubiquitin protein ligase
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA