Acetyl-alpha Tubulin (Lys40) Monoclonal Antibody (6-11B-1) | Invitrogen 32-2700 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Acetyl-alpha Tubulin (Lys40) Monoclonal Antibody (6-11B-1)

catalog :

32-2700

quantity :

100 µg

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6-11B-1

reactivity :

, Arthrotardigrada, human, mouse, rat, dogs, chicken, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay

more info or order :

Invitrogen product webpage

citations: 46

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:1000; loading ...; fig 1a	Oliazadeh N, Gorman K, Eveleigh R, Bourque G, Moreau A. Identification of Elongated Primary Cilia with Impaired Mechanotransduction in Idiopathic Scoliosis Patients. Sci Rep. 2017;7:44260 pubmed publisher
proximity ligation assay; human; 0.7 ug/ml; loading ...; fig 4a	Assis L, Silva Junior R, Dolce L, Alborghetti M, Honorato R, Nascimento A, et al. The molecular motor Myosin Va interacts with the cilia-centrosomal protein RPGRIP1L. Sci Rep. 2017;7:43692 pubmed publisher
immunocytochemistry; human; loading ...; fig 3b	Ramakrishnan S, Ku S, Ciamporcero E, Miles K, Attwood K, Chintala S, et al. HDAC 1 and 6 modulate cell invasion and migration in clear cell renal cell carcinoma. BMC Cancer. 2016;16:617 pubmed publisher
immunohistochemistry; chicken; 1:500; fig 1	Amarnath S, Agarwala S. Cell-cycle-dependent TGF?-BMP antagonism regulates neural tube closure by modulating tight junctions. J Cell Sci. 2017;130:119-131 pubmed publisher
immunohistochemistry; zebrafish ; 1:1000; fig 3	Podlasz P, Jakimiuk A, Chmielewska Krzesinska M, Kasica N, Nowik N, Kaleczyc J. Galanin regulates blood glucose level in the zebrafish: a morphological and functional study. Histochem Cell Biol. 2016;145:105-17 pubmed publisher
immunocytochemistry; mouse; fig 3	Eisner A, Pazyra Murphy M, Durresi E, Zhou P, Zhao X, Chadwick E, et al. The Eya1 phosphatase promotes Shh signaling during hindbrain development and oncogenesis. Dev Cell. 2015;33:22-35 pubmed publisher
immunohistochemistry; mouse; 1:300	Grati M, Chakchouk I, Ma Q, Bensaïd M, DeSmidt A, Turki N, et al. A missense mutation in DCDC2 causes human recessive deafness DFNB66, likely by interfering with sensory hair cell and supporting cell cilia length regulation. Hum Mol Genet. 2015;24:2482-91 pubmed publisher
immunocytochemistry; chicken; 1:300	San Miguel Ruiz J, Letourneau P. The role of Arp2/3 in growth cone actin dynamics and guidance is substrate dependent. J Neurosci. 2014;34:5895-908 pubmed publisher
immunohistochemistry; human; fig 1	Buffington D, Pino C, Chen L, Westover A, Hageman G, Humes H. Bioartificial Renal Epithelial Cell System (BRECS): A Compact, Cryopreservable Extracorporeal Renal Replacement Device. Cell Med. 2012;4:33-43 pubmed
immunohistochemistry; Arthrotardigrada; 1:300	Persson D, Halberg K, Jørgensen A, Møbjerg N, Kristensen R. Brain anatomy of the marine tardigrade Actinarctus doryphorus (Arthrotardigrada). J Morphol. 2014;275:173-90 pubmed publisher
immunocytochemistry; dogs; fig 2 immunohistochemistry - paraffin section; mouse; 1:250	Ott C, Elia N, Jeong S, Insinna C, Sengupta P, Lippincott Schwartz J. Primary cilia utilize glycoprotein-dependent adhesion mechanisms to stabilize long-lasting cilia-cilia contacts. Cilia. 2012;1:3 pubmed publisher
immunocytochemistry; human; fig 5	Roy E, Bruyère J, Flamant P, Bigou S, Ausseil J, Vitry S, et al. GM130 gain-of-function induces cell pathology in a model of lysosomal storage disease. Hum Mol Genet. 2012;21:1481-95 pubmed publisher
immunohistochemistry; zebrafish ; 1:500; fig s4	Doll C, Burkart J, Hope K, Halpern M, Gamse J. Subnuclear development of the zebrafish habenular nuclei requires ER translocon function. Dev Biol. 2011;360:44-57 pubmed publisher
immunocytochemistry; rat; 1:20; fig 5	Shao Y, Wang L, Welter J, Ballock R. Primary cilia modulate Ihh signal transduction in response to hydrostatic loading of growth plate chondrocytes. Bone. 2012;50:79-84 pubmed publisher
western blot; mouse; 1:500; fig s2	Lancaster M, Schroth J, Gleeson J. Subcellular spatial regulation of canonical Wnt signalling at the primary cilium. Nat Cell Biol. 2011;13:700-7 pubmed publisher
immunocytochemistry; dogs; 1:200; fig 3 immunocytochemistry; human; 1:200; fig 3	Ferrell N, Desai R, Fleischman A, Roy S, Humes H, Fissell W. A microfluidic bioreactor with integrated transepithelial electrical resistance (TEER) measurement electrodes for evaluation of renal epithelial cells. Biotechnol Bioeng. 2010;107:707-16 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:1000	Louie C, Caridi G, Lopes V, Brancati F, Kispert A, Lancaster M, et al. AHI1 is required for photoreceptor outer segment development and is a modifier for retinal degeneration in nephronophthisis. Nat Genet. 2010;42:175-80 pubmed publisher
immunocytochemistry; mouse; 1:500; fig 1	Lancaster M, Louie C, Silhavy J, Sintasath L, DeCambre M, Nigam S, et al. Impaired Wnt-beta-catenin signaling disrupts adult renal homeostasis and leads to cystic kidney ciliopathy. Nat Med. 2009;15:1046-54 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; fig 3	Cantagrel V, Silhavy J, Bielas S, Swistun D, Marsh S, Bertrand J, et al. Mutations in the cilia gene ARL13B lead to the classical form of Joubert syndrome. Am J Hum Genet. 2008;83:170-9 pubmed publisher
immunocytochemistry; human; 1:200; fig 3	Fissell W, Manley S, Westover A, Humes H, Fleischman A, Roy S. Differentiated growth of human renal tubule cells on thin-film and nanostructured materials. ASAIO J. 2006;52:221-7 pubmed
	Bosakova M, Abraham S, Nita A, Hruba E, Buchtová M, Taylor S, et al. Mutations in GRK2 cause Jeune syndrome by impairing Hedgehog and canonical Wnt signaling. EMBO Mol Med. 2020;12:e11739 pubmed publisher
	Frasca A, Spiombi E, Palmieri M, Albizzati E, Valente M, Bergo A, et al. MECP2 mutations affect ciliogenesis: a novel perspective for Rett syndrome and related disorders. EMBO Mol Med. 2020;12:e10270 pubmed publisher
	Duerr J, Leitz D, Szczygiel M, Dvornikov D, Fraumann S, Kreutz C, et al. Conditional deletion of Nedd4-2 in lung epithelial cells causes progressive pulmonary fibrosis in adult mice. Nat Commun. 2020;11:2012 pubmed publisher
	Yeo A, Henningham A, Fantino E, Galbraith S, Krause L, Wainwright C, et al. Increased susceptibility of airway epithelial cells from ataxia-telangiectasia to S. pneumoniae infection due to oxidative damage and impaired innate immunity. Sci Rep. 2019;9:2627 pubmed publisher
	Kunova Bosakova M, Nita A, Gregor T, Varecha M, Gudernová I, Fafilek B, et al. Fibroblast growth factor receptor influences primary cilium length through an interaction with intestinal cell kinase. Proc Natl Acad Sci U S A. 2019;: pubmed publisher
	Munro I, Garcia E, Yan M, Guldbrand S, Kumar S, Kwakwa K, et al. Accelerating single molecule localization microscopy through parallel processing on a high-performance computing cluster. J Microsc. 2019;273:148-160 pubmed publisher
	Love H, Ao M, Jorgensen S, Swearingen L, Ferrell N, Evans R, et al. Substrate Elasticity Governs Differentiation of Renal Tubule Cells in Prolonged Culture. Tissue Eng Part A. 2018;: pubmed publisher
	Hu Q, Guo L, Li J, Song C, Yu L, He D, et al. Deletion of Kncn Does Not Affect Kinocilium and Stereocilia Bundle Morphogenesis and Mechanotransduction in Cochlear Hair Cells. Front Mol Neurosci. 2018;11:326 pubmed publisher
	Schagen J, Sly P, Fantino E. Characterizing well-differentiated culture of primary human nasal epithelial cells for use in wound healing assays. Lab Invest. 2018;98:1478-1486 pubmed publisher
	Ramachandra Rao S, Pfeffer B, Más Gómez N, Skelton L, Keiko U, Sparrow J, et al. Compromised phagosome maturation underlies RPE pathology in cell culture and whole animal models of Smith-Lemli-Opitz Syndrome. Autophagy. 2018;14:1796-1817 pubmed publisher
	Vanaja G, Ramulu H, Kalle A. Overexpressed HDAC8 in cervical cancer cells shows functional redundancy of tubulin deacetylation with HDAC6. Cell Commun Signal. 2018;16:20 pubmed publisher
	Xie B, Laxman B, Hashemifar S, Stern R, Gilliam T, Maltsev N, et al. Chemokine expression in the early response to injury in human airway epithelial cells. PLoS ONE. 2018;13:e0193334 pubmed publisher
	Ott C, Nachmias D, Adar S, Jarnik M, Sherman S, Birnbaum R, et al. VPS4 is a dynamic component of the centrosome that regulates centrosome localization of Î³-tubulin, centriolar satellite stability and ciliogenesis. Sci Rep. 2018;8:3353 pubmed publisher
	Kunova Bosakova M, Varecha M, Hampl M, Durán I, Nita A, Buchtová M, et al. Regulation of ciliary function by fibroblast growth factor signaling identifies FGFR3-related disorders achondroplasia and thanatophoric dysplasia as ciliopathies. Hum Mol Genet. 2018;27:1093-1105 pubmed publisher
	Broix L, Asselin L, Silva C, Ivanova E, Tilly P, Gilet J, et al. Ciliogenesis and cell cycle alterations contribute to KIF2A-related malformations of cortical development. Hum Mol Genet. 2018;27:224-238 pubmed publisher
	Ferrell N, Cheng J, Miao S, Roy S, Fissell W. Orbital Shear Stress Regulates Differentiation and Barrier Function of Primary Renal Tubular Epithelial Cells. ASAIO J. 2017;: pubmed publisher
	Liu P, Weinreb V, Ridilla M, Betts L, Patel P, de Silva A, et al. Rapid, directed transport of DC-SIGN clusters in the plasma membrane. Sci Adv. 2017;3:eaao1616 pubmed publisher
	Hamel V, Steib E, Hamelin R, Armand F, Borgers S, Flückiger I, et al. Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis. Curr Biol. 2017;27:2486-2498.e6 pubmed publisher
	Paige Taylor S, Kunova Bosakova M, Varecha M, Balek L, Bárta T, Trantirek L, et al. An inactivating mutation in intestinal cell kinase, ICK, impairs hedgehog signalling and causes short rib-polydactyly syndrome. Hum Mol Genet. 2016;25:3998-4011 pubmed publisher
	Nandadasa S, Nelson C, Apte S. ADAMTS9-Mediated Extracellular Matrix Dynamics Regulates Umbilical Cord Vascular Smooth Muscle Differentiation and Rotation. Cell Rep. 2015;11:1519-28 pubmed publisher
	Retana C, Sanchez E, Gonzalez S, Perez Lopez A, Cruz A, Lagunas Muñoz J, et al. Retinoic acid improves morphology of cultured peritoneal mesothelial cells from patients undergoing dialysis. PLoS ONE. 2013;8:e79678 pubmed publisher
	Gharbiah M, Nakamoto A, Nagy L. Analysis of ciliary band formation in the mollusc Ilyanassa obsoleta. Dev Genes Evol. 2013;223:225-35 pubmed publisher
	Metscher B, Muller G. MicroCT for molecular imaging: quantitative visualization of complete three-dimensional distributions of gene products in embryonic limbs. Dev Dyn. 2011;240:2301-8 pubmed publisher
	Lancaster M, Gopal D, Kim J, Saleem S, Silhavy J, Louie C, et al. Defective Wnt-dependent cerebellar midline fusion in a mouse model of Joubert syndrome. Nat Med. 2011;17:726-31 pubmed publisher
	Mangold U, Hayakawa H, Coughlin M, Munger K, Zetter B. Antizyme, a mediator of ubiquitin-independent proteasomal degradation and its inhibitor localize to centrosomes and modulate centriole amplification. Oncogene. 2008;27:604-13 pubmed
	Szczypka M, Westover A, Clouthier S, Ferrara J, Humes H. Rare incorporation of bone marrow-derived cells into kidney after folic acid-induced injury. Stem Cells. 2005;23:44-54 pubmed

product information

Product Type :

Antibody

Product Name :

Acetyl-alpha Tubulin (Lys40) Monoclonal Antibody (6-11B-1)

Catalog # :

32-2700

Quantity :

100 µg

Price :

US 399.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat, Sea urchin

Applications :

Flow Cytometry: Assay-dependent, Immunocytochemistry: 1-3 µg/mL, Western Blot: 1-3 µg/mL

Species :

Human, Mouse, Rat, Sea urchin

Clone :

6-11B-1

Isotype :

IgG2b

Storage :

-20°C

Description :

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

Immunogen :

Acetylated alpha-tubulin from the axoneme of sea urchin sperm flagella.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunocytochemistry: 1-3 µg/mL, Western Blot: 1-3 µg/mL

Aliases :

&agr-tubulin 84B; 1t; a1t; ALPHA 84C; alpha tubulin; alpha tubulin 84B; alpha[[1]] tubulin; alpha[[1]]-tubulin; alpha1; alpha1 tubulin; alpha1t; alpha1tub; alpha1tub84B; alpha1-tubulin; alpha84B; alphaT; alphat-1; alphatub; alpha-tub; alphaTub1; alphatub84; alphaTUB84B; alpha-tub84B; alphaTub84B-PA; alphatubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; alpha-tubulin 4; Alpha-tubulin 6; alphaTubulin 84B; alpha-Tubulin 84B; alpha-Tubulin at 84B; alpha-tubulin I; Alpha-tubulin II; alpha-tubulin III; Alpha-tubulin isotype M-alpha-1; alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; alpha-tubulin isotype M-alpha-6; alpha-tubulin TUB1; alpha-tubulin ubiquitous; alphaTubulin84B; Alpha-Tubulin84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; AT25469p; aTub84B; a-tub84B; bA408E5.3; B-ALPHA-1; bcm948; BEST:LD32507; cb944; CG1913; CG1913-PA; chr3R:2914276. 2914416; clone 1.59; clone 2.30; Detyrosinated tubulin alpha-1A chain; Detyrosinated tubulin alpha-1B chain; Detyrosinated tubulin alpha-1C chain; Detyrosinated tubulin alpha-3 chain; Detyrosinated tubulin alpha-3C chain; Detyrosinated tubulin alpha-3D chain; Detyrosinated tubulin alpha-3E chain; DM1alpha; Dmel CG1913; Dmel_CG1913; DTA1; fb22g06; FLJ30169; H2 ALPHA; H2-ALPHA; hum-a-tub1; hum-a-tub2; I79_019110; K-ALPHA-1; l(3)84Bd; l(3)g3; lis3; LOC100726777; LOC100731885; LOC100857858; LOC101118307; LOC106557476; M[a]4; M[a]6; ms(3)nc33; nc33; RGD1565476; similar to alpha-tubulin isoform 1; similar to tubulin alpha 1; T; Talpha1; Talpha1 alpha-tubulin; TBA1_DROME; Testis-specific alpha-tubulin; Tub; Tub 84B; TUB1; Tub1C; Tub84B; TUBA1; Tuba-1; tuba1 protein; TUBA1/3/4; Tuba1a; tuba1a.L; tuba1a-a; tuba1a-b; TUBA1B; TUBA1C; tuba1l; tuba1l2; TUBA2; TUBA3; TUBA3C; TUBA3D; TUBA3E; Tuba4; Tuba4a; TUBA5; Tuba6; tuba8.S; tubA84B; tubal2; Tubalpha1; tubulin; tubulin 1 alpha-chain; tubulin 1alpha; tubulin alpha; tubulin alpha 1a; tubulin alpha 1a L homeolog; tubulin alpha 1b; tubulin alpha 1c; tubulin alpha 3; tubulin alpha 3c; tubulin alpha 3e; tubulin alpha 4a; tubulin alpha 6; tubulin alpha 8 S homeolog; tubulin alpha1; tubulin alpha-1 chain; tubulin alpha1 promoter; Tubulin alpha-1A chain; tubulin alpha-1B chain; Tubulin alpha-1C chain; tubulin alpha-1C chain-like; tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; tubulin alpha-3C/D chain; Tubulin alpha-3D chain; Tubulin alpha-3E chain; tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-5 chain; tubulin alpha-5 chain-like; tubulin alpha-6 chain; tubulin alpha-8 chain; tubulin alpha-ubiquitous chain; tubulin B-alpha-1; Tubulin H2-alpha; tubulin K-alpha-1; tubulin(alpha1); tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1, like; tubulin, alpha 1, like 2; tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 5; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin1alpha; tubulin84B; Tubulin-alpha1; Unknown (protein for MGC:133894); unnamed protein product; wu:fb22g06; wu:fb37a10; XELAEV_18019849mg; XELAEV_18020901mg; YML085C

more info or order :

Invitrogen product webpage