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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Cullin 1 Monoclonal Antibody (2H4C9)
catalog :
32-2400
quantity :
100 µg
price :
US 425.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2H4C9
reactivity :
human, mouse
application :
western blot, ELISA, immunoprecipitation
more info or order :
citations: 21
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig s10
Li J, Yakushi T, Parlati F, MacKinnon A, Pérez C, Ma Y, et al. Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11. Nat Chem Biol. 2017;13:486-493 pubmed publisher
  • western blot; human; 1:500; fig 1
Engel K, Rudelius M, Slawska J, Jacobs L, Ahangarian Abhari B, Altmann B, et al. USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma. EMBO Mol Med. 2016;8:851-62 pubmed publisher
  • western blot; mouse; 1:500; fig s4
  • western blot; human; 1:500; fig 1
Eichner R, Heider M, Fernández Sáiz V, van Bebber F, Garz A, Lemeer S, et al. Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity. Nat Med. 2016;22:735-43 pubmed publisher
  • western blot; human; fig 3
Mark K, Loveless T, Toczyski D. Isolation of ubiquitinated substrates by tandem affinity purification of E3 ligase-polyubiquitin-binding domain fusions (ligase traps). Nat Protoc. 2016;11:291-301 pubmed publisher
  • western blot; human; fig 5
Zhang C, Li X, Adelmant G, Dobbins J, Geisen C, Oser M, et al. Peptidic degron in EID1 is recognized by an SCF E3 ligase complex containing the orphan F-box protein FBXO21. Proc Natl Acad Sci U S A. 2015;112:15372-7 pubmed publisher
  • western blot; human; 1:5000
Sandoval D, Hill S, Ziemba A, Lewis S, Kuhlman B, Kleiger G. Ubiquitin-conjugating enzyme Cdc34 and ubiquitin ligase Skp1-cullin-F-box ligase (SCF) interact through multiple conformations. J Biol Chem. 2015;290:1106-18 pubmed publisher
  • western blot; human; 1:500
Honarpour N, Rose C, Brumbaugh J, Anderson J, Graham R, Sweredoski M, et al. F-box protein FBXL16 binds PP2A-B55? and regulates differentiation of embryonic stem cells along the FLK1+ lineage. Mol Cell Proteomics. 2014;13:780-91 pubmed publisher
  • immunoprecipitation; human; 5 ug; fig 2
Tiwari A, Schneider M, Fiorino A, Haider R, Okoniewski M, Roschitzki B, et al. Early insights into the function of KIAA1199, a markedly overexpressed protein in human colorectal tumors. PLoS ONE. 2013;8:e69473 pubmed publisher
  • western blot; human; 1:1000; fig 3
Rico Bautista E, Zhu W, Kitada S, Ganapathy S, Lau E, Krajewski S, et al. Small molecule-induced mitochondrial disruption directs prostate cancer inhibition via UPR signaling. Oncotarget. 2013;4:1212-29 pubmed
  • western blot; human; fig 1
Inuzuka H, Shaik S, Onoyama I, Gao D, Tseng A, Maser R, et al. SCF(FBW7) regulates cellular apoptosis by targeting MCL1 for ubiquitylation and destruction. Nature. 2011;471:104-9 pubmed publisher
  • western blot; human; fig s5
Guardavaccaro D, Frescas D, Dorrello N, Peschiaroli A, Multani A, Cardozo T, et al. Control of chromosome stability by the beta-TrCP-REST-Mad2 axis. Nature. 2008;452:365-9 pubmed publisher
Bailly A, Perrin A, Serrano Macia M, Maghames C, Leidecker O, Trauchessec H, et al. The Balance between Mono- and NEDD8-Chains Controlled by NEDP1 upon DNA Damage Is a Regulatory Module of the HSP70 ATPase Activity. Cell Rep. 2019;29:212-224.e8 pubmed publisher
Kuchay S, Saeed M, Giorgi C, Li J, Hoffmann H, Pinton P, et al. NS5A Promotes Constitutive Degradation of IP3R3 to Counteract Apoptosis Induced by Hepatitis C Virus. Cell Rep. 2018;25:833-840.e3 pubmed publisher
Liu X, Reitsma J, Mamrosh J, Zhang Y, Straube R, Deshaies R. Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression. Mol Cell. 2018;69:773-786.e6 pubmed publisher
Hosogane M, Bosu L, Fukumoto E, Yamada H, Sato S, Nakayama K. Geminin is an indispensable inhibitor of Cdt1 in mouse embryonic stem cells. Genes Cells. 2017;22:360-375 pubmed publisher
Hu Y, O Boyle K, Auer J, Raju S, You F, Wang P, et al. Multiple UBXN family members inhibit retrovirus and lentivirus production and canonical NFκΒ signaling by stabilizing IκBα. PLoS Pathog. 2017;13:e1006187 pubmed publisher
Duda D, Olszewski J, Tron A, Hammel M, Lambert L, Waddell M, et al. Structure of a glomulin-RBX1-CUL1 complex: inhibition of a RING E3 ligase through masking of its E2-binding surface. Mol Cell. 2012;47:371-82 pubmed publisher
Bandau S, Knebel A, Gage Z, Wood N, Alexandru G. UBXN7 docks on neddylated cullin complexes using its UIM motif and causes HIF1? accumulation. BMC Biol. 2012;10:36 pubmed publisher
Tron A, Arai T, Duda D, Kuwabara H, Olszewski J, Fujiwara Y, et al. The glomuvenous malformation protein Glomulin binds Rbx1 and regulates cullin RING ligase-mediated turnover of Fbw7. Mol Cell. 2012;46:67-78 pubmed publisher
Sohn C, Agnew H, Lee J, Sweredoski M, Graham R, Smith G, et al. Designer reagents for mass spectrometry-based proteomics: clickable cross-linkers for elucidation of protein structures and interactions. Anal Chem. 2012;84:2662-9 pubmed publisher
Huotari J, Meyer Schaller N, Hubner M, Stauffer S, Katheder N, Horvath P, et al. Cullin-3 regulates late endosome maturation. Proc Natl Acad Sci U S A. 2012;109:823-8 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Cullin 1 Monoclonal Antibody (2H4C9)
Catalog # :
32-2400
Quantity :
100 µg
Price :
US 425.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human
Applications :
ELISA: 0.1-1.0 µg/mL, Immunoprecipitation: 5-10 µg, Western Blot: 1-2 µg/mL
Species :
Human
Clone :
2H4C9
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
CUL1 gene ontology annotations related to this gene include animal organ morphogenesis; apoptotic process; cell proliferation; protein monoubiquitination; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process.
Immunogen :
A synthetic peptide corresponding to an 11 amino acid sequence at the C-terminus of the human CUL-1 protein.
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-1.0 µg/mL, Immunoprecipitation: 5-10 µg, Western Blot: 1-2 µg/mL
Aliases :
Cul1; CUL-1; cullin 1; cullin-1
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA