Nitrotyrosine Monoclonal Antibody (HM11) | Invitrogen 32-1900 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Nitrotyrosine Monoclonal Antibody (HM11)

catalog :

32-1900

quantity :

100 ug

price :

US 520.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HM11

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

citations: 23

Reference
Abbah J, Vacher C, Goldstein E, Li Z, Kundu S, Talbot B, et al. Oxidative Stress-Induced Damage to the Developing Hippocampus Is Mediated by GSK3β. J Neurosci. 2022;42:4812-4827 pubmed publisher
Léger T, He B, Azarnoush K, Jouve C, Rigaudière J, Joffre F, et al. Dietary EPA Increases Rat Mortality in Diabetes Mellitus, A Phenomenon Which Is Compensated by Green Tea Extract. Antioxidants (Basel). 2019;8: pubmed publisher
Léger T, Azarnoush K, Traore A, Cassagnes L, Rigaudière J, Jouve C, et al. Antioxidant and Cardioprotective Effects of EPA on Early Low-Severity Sepsis through UCP3 and SIRT3 Upholding of the Mitochondrial Redox Potential. Oxid Med Cell Longev. 2019;2019:9710352 pubmed publisher
Garcia Esparcia P, Koneti A, Rodríguez Oroz M, Gago B, del Río J, Ferrer I. Mitochondrial activity in the frontal cortex area 8 and angular gyrus in Parkinson's disease and Parkinson's disease with dementia. Brain Pathol. 2018;28:43-57 pubmed publisher
Degendorfer G, Chuang C, Kawasaki H, Hammer A, Malle E, Yamakura F, et al. Peroxynitrite-mediated oxidation of plasma fibronectin. Free Radic Biol Med. 2016;97:602-615 pubmed publisher
Degendorfer G, Chuang C, Hammer A, Malle E, Davies M. Peroxynitrous acid induces structural and functional modifications to basement membranes and its key component, laminin. Free Radic Biol Med. 2015;89:721-33 pubmed publisher
Seeley K, Fertig A, Dufresne C, Pinho J, Stevens S. Evaluation of a method for nitrotyrosine site identification and relative quantitation using a stable isotope-labeled nitrated spike-in standard and high resolution fourier transform MS and MS/MS analysis. Int J Mol Sci. 2014;15:6265-85 pubmed publisher
Tamplenizza M, Lenardi C, Maffioli E, Nonnis S, Negri A, Forti S, et al. Nitric oxide synthase mediates PC12 differentiation induced by the surface topography of nanostructured TiO2. J Nanobiotechnology. 2013;11:35 pubmed publisher
Del Bigio M, Khan O, da Silva Lopes L, Juliet P. Cerebral white matter oxidation and nitrosylation in young rodents with kaolin-induced hydrocephalus. J Neuropathol Exp Neurol. 2012;71:274-88 pubmed publisher
Lam S, Liu Y, Ng K, Lau C, Liong E, Tipoe G, et al. Chronic intermittent hypoxia induces local inflammation of the rat carotid body via functional upregulation of proinflammatory cytokine pathways. Histochem Cell Biol. 2012;137:303-17 pubmed publisher
Untersmayr E, Diesner S, Oostingh G, Selzle K, Pfaller T, Schultz C, et al. Nitration of the egg-allergen ovalbumin enhances protein allergenicity but reduces the risk for oral sensitization in a murine model of food allergy. PLoS ONE. 2010;5:e14210 pubmed publisher
Sigala F, Kotsinas A, Savari P, Filis K, Markantonis S, Iliodromitis E, et al. Oxidized LDL in human carotid plaques is related to symptomatic carotid disease and lesion instability. J Vasc Surg. 2010;52:704-13 pubmed publisher
Kennett E, Rees M, Malle E, Hammer A, Whitelock J, Davies M. Peroxynitrite modifies the structure and function of the extracellular matrix proteoglycan perlecan by reaction with both the protein core and the heparan sulfate chains. Free Radic Biol Med. 2010;49:282-93 pubmed publisher
Fu P, Birukova A, Xing J, Sammani S, Murley J, Garcia J, et al. Amifostine reduces lung vascular permeability via suppression of inflammatory signalling. Eur Respir J. 2009;33:612-24 pubmed publisher
Vaninetti N, Geldenhuys L, Porter G, Risch H, Hainaut P, Guernsey D, et al. Inducible nitric oxide synthase, nitrotyrosine and p53 mutations in the molecular pathogenesis of Barrett's esophagus and esophageal adenocarcinoma. Mol Carcinog. 2008;47:275-85 pubmed
Yeo M, Kim D, Cho S, Hong H. Ginseng, the root of Panax ginseng C.A. Meyer, protects ethanol-induced gastric damages in rat through the induction of cytoprotective heat-shock protein 27. Dig Dis Sci. 2008;53:606-13 pubmed
Olmos A, Giner R, Recio M, Rios J, Cerdá Nicolás J, Máñez S. Effects of plant alkylphenols on cytokine production, tyrosine nitration and inflammatory damage in the efferent phase of contact hypersensitivity. Br J Pharmacol. 2007;152:366-73 pubmed
Silva Miranda M, Rodríguez K, Martínez Cordero E, Rojas Espinosa O. Expression of cyclooxygenase-2, alpha 1-acid-glycoprotein and inducible nitric oxide synthase in the developing lesions of murine leprosy. Int J Exp Pathol. 2006;87:485-94 pubmed
Wada K, Nakajima A, Takahashi H, Yoneda M, Fujisawa N, Ohsawa E, et al. Protective effect of endogenous PPARgamma against acute gastric mucosal lesions associated with ischemia-reperfusion. Am J Physiol Gastrointest Liver Physiol. 2004;287:G452-8 pubmed
Cho W, Chae C. Evidence of nitric oxide synthase 2 activity in swine naturally infected with Actinobacillus pleuropneumoniae. Vet Pathol. 2003;40:276-82 pubmed
Irie Y, Saeki M, Kamisaki Y, Martin E, Murad F. Histone H1.2 is a substrate for denitrase, an activity that reduces nitrotyrosine immunoreactivity in proteins. Proc Natl Acad Sci U S A. 2003;100:5634-9 pubmed
Montalto M, Hart M, Jordan J, Wada K, Stahl G. Role for complement in mediating intestinal nitric oxide synthase-2 and superoxide dismutase expression. Am J Physiol Gastrointest Liver Physiol. 2003;285:G197-206 pubmed
Newman D, Hoffman S, Kotamraju S, Zhao T, Wakim B, Kalyanaraman B, et al. Nitration of PECAM-1 ITIM tyrosines abrogates phosphorylation and SHP-2 binding. Biochem Biophys Res Commun. 2002;296:1171-9 pubmed

product information

Product Type :

Antibody

Product Name :

Nitrotyrosine Monoclonal Antibody (HM11)

Catalog # :

32-1900

Quantity :

100 ug

Price :

US 520.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Chemical

Applications :

Immunohistochemistry: 1-2 ug/mL, Western Blot: 1-2 ug/mL

Species :

Chemical

Clone :

HM11

Isotype :

IgG1

Storage :

-20 C

Description :

Nitrotyrosine is a relatively stable product formed from various reaction pathways. Perhaps most notable is the reaction of peroxynitrite (formed from superoxide and nitric oxide (NO) radicals) with tyrosine. As a strong oxidant and nitrating agent, peroxynitrite mediates tyrosine nitration reactions on proteins resulting in inactivation of certain housekeeping enzymes (e. g., alpha1-antiproteinase) as well as endogenous antioxidant enzymes such as catalase and superoxide dismutase (SOD). Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Nitrotyrosine has been implicated in the pathogenesis of several inflammatory, infectious, and degenerative human diseases, such as Alzheimer's and Parkinson's disease, multiple sclerosis, amyotrophic lateral sclerosis (ALS), asthma, atherosclerosis, and a variety of conditions precipitated by endothelial injury. At the protein level, tyrosine nitration can lead to loss or alteration of protein function, as demonstrated for the oncogenic protein p53 and the mitochondrial protein, manganese-dependent superoxide dismutase (MnSOD).

Immunogen :

Nitrotyrosine attached to a carrier protein

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry: 1-2 ug/mL, Western Blot: 1-2 ug/mL

Aliases :

Nitro-tyrosine