CDH11 Monoclonal Antibody (5B2H5) | Invitrogen 32-1700 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CDH11 Monoclonal Antibody (5B2H5)

catalog :

32-1700

quantity :

100 ug

price :

US 520.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5B2H5

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunocytochemistry knockout validation

more info or order :

Invitrogen product webpage

citations: 46

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry knockout validation; mouse; fig 1	Agarwal S, Lee D, Kiener H, Brenner M. Coexpression of two mesenchymal cadherins, cadherin 11 and N-cadherin, on murine fibroblast-like synoviocytes. Arthritis Rheum. 2008;58:1044-54 pubmed publisher
immunocytochemistry; mouse; 1:50; loading ...; fig 4b western blot; mouse; 1:1000; loading ...; fig 5a	Beckmann D, Römer Hillmann A, Krause A, Hansen U, Wehmeyer C, Intemann J, et al. Lasp1 regulates adherens junction dynamics and fibroblast transformation in destructive arthritis. Nat Commun. 2021;12:3624 pubmed publisher
immunohistochemistry; human; 1:500; loading ...; fig s9a	Ferrer Torres D, Nancarrow D, Kuick R, Thomas D, Nadal E, Lin J, et al. Genomic similarity between gastroesophageal junction and esophageal Barrett's adenocarcinomas. Oncotarget. 2016;7:54867-54882 pubmed publisher
western blot; human; fig 4	Kourtidis A, Anastasiadis P. PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications. Cell Cycle. 2016;15:498-505 pubmed publisher
immunoprecipitation; human western blot; human	Noss E, Watts G, Zocco D, Keller T, Whitman M, Blobel C, et al. Evidence for cadherin-11 cleavage in the synovium and partial characterization of its mechanism. Arthritis Res Ther. 2015;17:126 pubmed publisher
western blot; human; 1:150	Ding X, Zhang Y, Huang Y, Liu S, Lu H, Sun T. Cadherin-11 involves in synovitis and increases the migratory and invasive capacity of fibroblast-like synoviocytes of osteoarthritis. Int Immunopharmacol. 2015;26:153-61 pubmed publisher
western blot; human; 1:500	Sun Q, Cibas E, Huang H, Hodgson L, Overholtzer M. Induction of entosis by epithelial cadherin expression. Cell Res. 2014;24:1288-98 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100	Schulte J, Srikanth M, Das S, Zhang J, Lathia J, Yin L, et al. Cadherin-11 regulates motility in normal cortical neural precursors and glioblastoma. PLoS ONE. 2013;8:e70962 pubmed publisher
western blot; human; fig 1	Law M, Corsino P, Jahn S, Davis B, Chen S, Patel B, et al. Glucocorticoids and histone deacetylase inhibitors cooperate to block the invasiveness of basal-like breast cancer cells through novel mechanisms. Oncogene. 2013;32:1316-29 pubmed publisher
western blot; human; fig 2	Carmona F, Villanueva A, Vidal A, Muñoz C, Puertas S, Penin R, et al. Epigenetic disruption of cadherin-11 in human cancer metastasis. J Pathol. 2012;228:230-40 pubmed publisher
immunocytochemistry; human; fig 5 western blot; human; fig 5	Li L, Ying J, Li H, Zhang Y, Shu X, Fan Y, et al. The human cadherin 11 is a pro-apoptotic tumor suppressor modulating cell stemness through Wnt/?-catenin signaling and silenced in common carcinomas. Oncogene. 2012;31:3901-12 pubmed publisher
immunocytochemistry; human; fig 5 western blot; human; fig 5	Strakovsky R, Pan Y. A decrease in DKK1, a WNT inhibitor, contributes to placental lipid accumulation in an obesity-prone rat model. Biol Reprod. 2012;86:81 pubmed publisher
immunocytochemistry; mouse; 10 ug/ml; fig 2 western blot; mouse; 1:500; fig 2	Schneider D, Wu M, Le T, Cho S, Brenner M, Blackburn M, et al. Cadherin-11 contributes to pulmonary fibrosis: potential role in TGF-? production and epithelial to mesenchymal transition. FASEB J. 2012;26:503-12 pubmed publisher
immunohistochemistry - paraffin section; human; fig 4	Ekwall A, Eisler T, Anderberg C, Jin C, Karlsson N, Brisslert M, et al. The tumour-associated glycoprotein podoplanin is expressed in fibroblast-like synoviocytes of the hyperplastic synovial lining layer in rheumatoid arthritis. Arthritis Res Ther. 2011;13:R40 pubmed publisher
immunohistochemistry - paraffin section; human; 1:10; tbl 2	Wu J, Beck A, Pate L, Witten D, Zhu S, Montgomery K, et al. Endogenous versus tumor-specific host response to breast carcinoma: a study of stromal response in synchronous breast primaries and biopsy site changes. Clin Cancer Res. 2011;17:437-46 pubmed publisher
immunohistochemistry - paraffin section; human; 1:10	Webster J, Beck A, Sharma M, Espinosa I, Weigelt B, Schreuder M, et al. Variations in stromal signatures in breast and colorectal cancer metastases. J Pathol. 2010;222:158-65 pubmed publisher
immunohistochemistry - paraffin section; human; fig 7 western blot; human; fig s7 immunohistochemistry - paraffin section; mouse; fig 7	Hakuno D, Kimura N, Yoshioka M, Mukai M, Kimura T, Okada Y, et al. Periostin advances atherosclerotic and rheumatic cardiac valve degeneration by inducing angiogenesis and MMP production in humans and rodents. J Clin Invest. 2010;120:2292-306 pubmed publisher
western blot; human; fig 5	Walenda T, Bork S, Horn P, Wein F, Saffrich R, Diehlmann A, et al. Co-culture with mesenchymal stromal cells increases proliferation and maintenance of haematopoietic progenitor cells. J Cell Mol Med. 2010;14:337-50 pubmed publisher
western blot; human; fig 1	Farina A, Bong Y, Feltes C, Byers S. Post-transcriptional regulation of cadherin-11 expression by GSK-3 and beta-catenin in prostate and breast cancer cells. PLoS ONE. 2009;4:e4797 pubmed publisher
western blot; human; fig 3	Li Z, Zhou Z, Welch D, Donahue H. Expressing connexin 43 in breast cancer cells reduces their metastasis to lungs. Clin Exp Metastasis. 2008;25:893-901 pubmed publisher
immunohistochemistry - frozen section; human; fig 1 western blot; human; 0.5 ug/ml; fig 3	Vandooren B, Cantaert T, ter Borg M, Noordenbos T, Kuhlman R, Gerlag D, et al. Tumor necrosis factor alpha drives cadherin 11 expression in rheumatoid inflammation. Arthritis Rheum. 2008;58:3051-62 pubmed publisher
western blot; human	Chu K, Cheng C, Ye X, Lee Y, Zurita A, Chen D, et al. Cadherin-11 promotes the metastasis of prostate cancer cells to bone. Mol Cancer Res. 2008;6:1259-67 pubmed publisher
western blot; human; fig 1A	Bellahcene A, Bachelier R, Detry C, Lidereau R, Clezardin P, Castronovo V. Transcriptome analysis reveals an osteoblast-like phenotype for human osteotropic breast cancer cells. Breast Cancer Res Treat. 2007;101:135-48 pubmed
western blot; human; 1 ug/ml; fig 3	Yanagisawa M, Anastasiadis P. p120 catenin is essential for mesenchymal cadherin-mediated regulation of cell motility and invasiveness. J Cell Biol. 2006;174:1087-96 pubmed
immunohistochemistry; human; fig 1	Sarrio D, Moreno Bueno G, Sánchez Estevez C, Bañón Rodríguez I, Hernández Cortés G, Hardisson D, et al. Expression of cadherins and catenins correlates with distinct histologic types of ovarian carcinomas. Hum Pathol. 2006;37:1042-9 pubmed
western blot; mouse; 1:1000; fig 7	Selesniemi K, Reedy M, Gultice A, Guilbert L, Brown T. Transforming growth factor-beta induces differentiation of the labyrinthine trophoblast stem cell line SM10. Stem Cells Dev. 2005;14:697-711 pubmed
immunohistochemistry; mouse; fig 6	Inagaki M, Irie K, Ishizaki H, Tanaka Okamoto M, Morimoto K, Inoue E, et al. Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development. Development. 2005;132:1525-37 pubmed
western blot; human; 1:1000	Kapoor P, Saunders M, Li Z, Zhou Z, Sheaffer N, Kunze E, et al. Breast cancer metastatic potential: correlation with increased heterotypic gap junctional intercellular communication between breast cancer cells and osteoblastic cells. Int J Cancer. 2004;111:693-7 pubmed
immunocytochemistry; chicken; fig 2	Gerhart J, Neely C, Stewart B, Perlman J, Beckmann D, Wallon M, et al. Epiblast cells that express MyoD recruit pluripotent cells to the skeletal muscle lineage. J Cell Biol. 2004;164:739-46 pubmed
immunoprecipitation; human; fig 5 western blot; human; fig 1	Feltes C, Kudo A, Blaschuk O, Byers S. An alternatively spliced cadherin-11 enhances human breast cancer cell invasion. Cancer Res. 2002;62:6688-97 pubmed
	Sebastian A, Martin K, Peran I, Hum N, Leon N, Amiri B, et al. Loss of Cadherin-11 in pancreatic ductal adenocarcinoma alters tumor-immune microenvironment. Front Oncol. 2023;13:1286861 pubmed publisher
	Maruyama T, Hasegawa D, Valenta T, Haigh J, Bouchard M, Basler K, et al. GATA3 mediates nonclassical β-catenin signaling in skeletal cell fate determination and ectopic chondrogenesis. Sci Adv. 2022;8:eadd6172 pubmed publisher
	Li J, Cao R, Wang Q, Shi H, Wu Y, Sun K, et al. Cadherin-11 promotes the mechanical strength of engineered elastic cartilage by enhancing extracellular matrix synthesis and microstructure. J Tissue Eng Regen Med. 2022;16:188-199 pubmed publisher
	Yamamoto A, Saito T, Hosoya T, Kawahata K, Asano Y, Sato S, et al. Therapeutic effect of cyclin-dependent kinase 4/6 inhibitor on dermal fibrosis in murine models of systemic sclerosis. Arthritis Rheumatol. 2021;: pubmed publisher
	Frei J, Niescier R, Bridi M, Durens M, Nestor J, Kilander M, et al. Regulation of Neural Circuit Development by Cadherin-11 Provides Implications for Autism. Eneuro. 2021;8: pubmed publisher
	Gee T, Richards J, Mahmut A, Butcher J. Valve endothelial-interstitial interactions drive emergent complex calcific lesion formation in vitro. Biomaterials. 2021;269:120669 pubmed publisher
	Manohar S, Camacho Magallanes A, Echeverria C, Rogers C. Cadherin-11 Is Required for Neural Crest Specification and Survival. Front Physiol. 2020;11:563372 pubmed publisher
	Miao Y, Tian L, Martin M, Paige S, Galdos F, Li J, et al. Intrinsic Endocardial Defects Contribute to Hypoplastic Left Heart Syndrome. Cell Stem Cell. 2020;27:574-589.e8 pubmed publisher
	Satriyo P, Bamodu O, Chen J, Aryandono T, Haryana S, Yeh C, et al. Cadherin 11 Inhibition Downregulates β-catenin, Deactivates the Canonical WNT Signalling Pathway and Suppresses the Cancer Stem Cell-Like Phenotype of Triple Negative Breast Cancer. J Clin Med. 2019;8: pubmed publisher
	Domke L, Franke W. The cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions-a novel architectonic cell-cell junction system. Cell Tissue Res. 2019;375:451-482 pubmed publisher
	Zhu Q, Wang Z, Zhou L, Ren Y, Gong Y, Qin W, et al. The role of cadherin-11 in microcystin-LR-induced migration and invasion in colorectal carcinoma cells. Oncol Lett. 2018;15:1417-1422 pubmed publisher
	Mills F, Globa A, Liu S, Cowan C, Mobasser M, Phillips A, et al. Cadherins mediate cocaine-induced synaptic plasticity and behavioral conditioning. Nat Neurosci. 2017;20:540-549 pubmed publisher
	Satcher R, Pan T, Bilen M, Li X, Lee Y, Ortiz A, et al. Cadherin-11 endocytosis through binding to clathrin promotes cadherin-11-mediated migration in prostate cancer cells. J Cell Sci. 2015;128:4629-41 pubmed publisher
	Sharma M, Beck A, Webster J, Espinosa I, Montgomery K, Varma S, et al. Analysis of stromal signatures in the tumor microenvironment of ductal carcinoma in situ. Breast Cancer Res Treat. 2010;123:397-404 pubmed publisher
	Chu Y, Eder O, Thomas W, Simcha I, Pincet F, Ben Ze ev A, et al. Prototypical type I E-cadherin and type II cadherin-7 mediate very distinct adhesiveness through their extracellular domains. J Biol Chem. 2006;281:2901-10 pubmed
	Straub B, Boda J, Kuhn C, Schnoelzer M, Korf U, Kempf T, et al. A novel cell-cell junction system: the cortex adhaerens mosaic of lens fiber cells. J Cell Sci. 2003;116:4985-95 pubmed

product information

Product Type :

Antibody

Product Name :

CDH11 Monoclonal Antibody (5B2H5)

Catalog # :

32-1700

Quantity :

100 ug

Price :

US 520.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Immunohistochemistry: 5-10 ug/mL, Immunoprecipitation: 5 ug, Western Blot: 2-4 ug/mL

Species :

Human, Mouse

Clone :

5B2H5

Isotype :

IgG1, kappa

Storage :

-20 C

Description :

This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. All cadherins are single-pass transmembrane proteins with a variable number of 110 amino acid extracellular cadherin (EC) domains. Classical cadherins contain five large N-terminal EC domains, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Based on amino acid alignment, classical cadherins are divided into type I and type II subgroups. Type I cadherins, including cadherins E (epithelial), N (neural), P (placental), and R (retinal) cadherin differ from type I cadherins in their specific amino acid sequences. Type II cadherins include human cadherin-5, -6, -8, -11, and -12. Type II cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Cadherin-11 (OB-Cadherin) is a marker of the loosely connected and migratory cellular elements of the mesenchyme. Strong expression of Cadherin-11 has been noted in brain, spinal cord, bone marrow and bone cells, and is regulated in human endometrial glandular epithelial and stromal cells. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. A variant Cadherin-11 gene (variant form) encodes a truncated protein with an unusual cytoplasmic domain not present in other cadherins. The variant protein is generated by alternative splicing, utilizing a 179 bp intronic sequence. Recently, Cadherin-11 mRNA and protein were found to be expressed in most invasive breast cancer cell lines tested but not in non-invasive cell lines, suggesting that Cadherin-11 may be a marker for aggressive, invasive tumor subsets.

Immunogen :

Recombinant protein derived from an intracellular sequence of the human Cadherin 11 protein.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry: 5-10 ug/mL, Immunoprecipitation: 5 ug, Western Blot: 2-4 ug/mL

Aliases :

CAD11; Cad-11; Cadherin; cadherin 11; cadherin 11, type 2, OB-cadherin (osteoblast); cadherin-11; Cdh11; CDHOB; OB; OB Cadherin; OB-cadherin; OSF-4; Osteoblast cadherin; osteoblast-cadherin

more info or order :

Invitrogen product webpage