Cyclin E Monoclonal Antibody (HE12) | Invitrogen 32-1600 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cyclin E Monoclonal Antibody (HE12)

catalog :

32-1600

quantity :

200 µg

price :

375 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HE12

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 2	Duchnowska R, Wysocki P, Korski K, Czartoryska ArÅ‚ukowicz B, NiwiÅ„ska A, Orlikowska M, et al. Immunohistochemical prediction of lapatinib efficacy in advanced HER2-positive breast cancer patients. Oncotarget. 2016;7:550-64 pubmed publisher
flow cytometry; human; fig 3	Gagou M, Ganesh A, Thompson R, Phear G, Sanders C, Meuth M. Suppression of apoptosis by PIF1 helicase in human tumor cells. Cancer Res. 2011;71:4998-5008 pubmed publisher
western blot; human; fig 9	Korfali N, Srsen V, Waterfall M, Batrakou D, Pekovic V, Hutchison C, et al. A flow cytometry-based screen of nuclear envelope transmembrane proteins identifies NET4/Tmem53 as involved in stress-dependent cell cycle withdrawal. PLoS ONE. 2011;6:e18762 pubmed publisher
western blot; human; 0.5 ug/ml; fig s2	Lofmark S, de Klerk N, Aro H. Neisseria gonorrhoeae infection induces altered amphiregulin processing and release. PLoS ONE. 2011;6:e16369 pubmed publisher
western blot; human; 1:200; fig 6e	Rico Bautista E, Yang C, Lu L, Roth G, Wolf D. Chemical genetics approach to restoring p27Kip1 reveals novel compounds with antiproliferative activity in prostate cancer cells. BMC Biol. 2010;8:153 pubmed publisher
western blot; human; 1 ug/ml; fig 2	Chimploy K, Díaz G, Li Q, Carter O, Dashwood W, Mathews C, et al. E2F4 and ribonucleotide reductase mediate S-phase arrest in colon cancer cells treated with chlorophyllin. Int J Cancer. 2009;125:2086-94 pubmed publisher
western blot; human	Al Ayyoubi S, Gali Muhtasib H. Differential apoptosis by gallotannin in human colon cancer cells with distinct p53 status. Mol Carcinog. 2007;46:176-86 pubmed
western blot; human; 2.6 ug/ml; fig 4	Jones A, Jonsson A, Aro H. Neisseria gonorrhoeae infection causes a G1 arrest in human epithelial cells. FASEB J. 2007;21:345-55 pubmed
western blot; human; 1:200	Lu L, Schulz H, Wolf D. The F-box protein SKP2 mediates androgen control of p27 stability in LNCaP human prostate cancer cells. BMC Cell Biol. 2002;3:22 pubmed
	Gookin S, Min M, Phadke H, Chung M, Moser J, Miller I, et al. A map of protein dynamics during cell-cycle progression and cell-cycle exit. PLoS Biol. 2017;15:e2003268 pubmed publisher
	Giordano T, Kuick R, Else T, Gauger P, Vinco M, Bauersfeld J, et al. Molecular classification and prognostication of adrenocortical tumors by transcriptome profiling. Clin Cancer Res. 2009;15:668-76 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Cyclin E Monoclonal Antibody (HE12)

Catalog # :

32-1600

Quantity :

200 µg

Price :

375 USD

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

Immunocytochemistry: Assay Dependent, Immunohistochemistry: Assay Dependent, Immunoprecipitation: 1 µg, Western Blot: 1-2 µg/mL

Species :

Human

Clone :

HE12

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

Cyclin E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

Immunogen :

Full-length, recombinant, human cyclin E protein.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: Assay Dependent, Immunohistochemistry: Assay Dependent, Immunoprecipitation: 1 µg, Western Blot: 1-2 µg/mL

Aliases :

AW538188; CCNE; Ccne1; CycE1; CYCLE; cyclin E; cyclin E1; cyclin Es; cyclin Et; E-S; G1/S-specific cyclin-E1; pCCNE1

more info or order :

Invitrogen product webpage