Cyclin E Monoclonal Antibody (HE172) | Invitrogen 32-1500 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cyclin E Monoclonal Antibody (HE172)

catalog :

32-1500

quantity :

200 µg

price :

375 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HE172

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 4	Podmirseg S, JÃ¤kel H, Ranches G, Kullmann M, Sohm B, Villunger A, et al. Caspases uncouple p27(Kip1) from cell cycle regulated degradation and abolish its ability to stimulate cell migration and invasion. Oncogene. 2016;35:4580-90 pubmed publisher
	Christensen H, Trotter C, Hickman M, Edmunds W. Re-evaluating cost effectiveness of universal meningitis vaccination (Bexsero) in England: modelling study. BMJ. 2014;349:g5725 pubmed publisher
	Mehdipour P, Kheirollahi M, Mehrazin M, Kamalian N, Atri M. Evolutionary hypothesis of telomere length in primary breast cancer and brain tumour patients: a tracer for genomic-tumour heterogeneity and instability. Cell Biol Int. 2011;35:915-25 pubmed publisher
	Tudzarova S, Trotter M, Wollenschlaeger A, Mulvey C, Godovac Zimmermann J, Williams G, et al. Molecular architecture of the DNA replication origin activation checkpoint. EMBO J. 2010;29:3381-94 pubmed publisher
	Zhou D, Conrad C, Xia F, Park J, Payer B, Yin Y, et al. Mst1 and Mst2 maintain hepatocyte quiescence and suppress hepatocellular carcinoma development through inactivation of the Yap1 oncogene. Cancer Cell. 2009;16:425-38 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Cyclin E Monoclonal Antibody (HE172)

Catalog # :

32-1500

Quantity :

200 µg

Price :

375 USD

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

Gel Shift: Assay Dependent, Immunocytochemistry: Assay Dependent, Immunoprecipitation: 1 µg, Western Blot: 1-2 µg/mL

Species :

Human

Clone :

HE172

Isotype :

IgG2b, kappa

Storage :

-20°C

Description :

Cyclin E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

Immunogen :

This antibody was prepared against full-length, recombinant, human cyclin E protein.

Format :

Liquid

Applications w/Dilutions :

Gel Shift: Assay Dependent, Immunocytochemistry: Assay Dependent, Immunoprecipitation: 1 µg, Western Blot: 1-2 µg/mL

Aliases :

AW538188; CCNE; Ccne1; CycE1; CYCLE; cyclin E; cyclin E1; cyclin Es; cyclin Et; E-S; G1/S-specific cyclin-E1; pCCNE1

more info or order :

Invitrogen product webpage