E2F1 Monoclonal Antibody (KH95) | Invitrogen 32-1400 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

E2F1 Monoclonal Antibody (KH95)

catalog :

32-1400

quantity :

100 µg

price :

425 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

KH95

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 5b western blot knockout validation; mouse; loading ...; fig 3d	Choiniere J, Wu J, Wang L. Pyruvate Dehydrogenase Kinase 4 Deficiency Results in Expedited Cellular Proliferation through E2F1-Mediated Increase of Cyclins. Mol Pharmacol. 2017;91:189-196 pubmed publisher
immunoprecipitation; human	Oliveira A, Beyer G, Chugh R, Skube S, Majumder K, Banerjee S, et al. Triptolide abrogates growth of colon cancer and induces cell cycle arrest by inhibiting transcriptional activation of E2F. Lab Invest. 2015;95:648-659 pubmed publisher
immunohistochemistry - paraffin section; human; 1:200	Silva A, Santos A, Farfel J, Grinberg L, Ferretti R, Campos A, et al. Repair of oxidative DNA damage, cell-cycle regulation and neuronal death may influence the clinical manifestation of Alzheimer's disease. PLoS ONE. 2014;9:e99897 pubmed publisher
immunohistochemistry - paraffin section; human; 1:200	Ahn S, Jang S, Shim J, Kim D, Hong S, Sung C, et al. Genomic portrait of resectable hepatocellular carcinomas: implications of RB1 and FGF19 aberrations for patient stratification. Hepatology. 2014;60:1972-82 pubmed publisher
immunohistochemistry - paraffin section; human; 1:20	Jung Y, Joo K, Seong D, Choi Y, Kong D, Kim Y, et al. Identification of prognostic biomarkers for glioblastomas using protein expression profiling. Int J Oncol. 2012;40:1122-32 pubmed publisher
chromatin immunoprecipitation; mouse; fig 7 western blot; mouse; fig 6	Wallace D, Cotter T. Histone deacetylase activity in conjunction with E2F-1 and p53 regulates Apaf-1 expression in 661W cells and the retina. J Neurosci Res. 2009;87:887-905 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; fig 2	Lee J, Park C, Park J, Lim T, Park Y, Lim H, et al. Impact of E2F-1 expression on clinical outcome of gastric adenocarcinoma patients with adjuvant chemoradiation therapy. Clin Cancer Res. 2008;14:82-8 pubmed publisher
western blot; mouse	Kawagoe H, Kandilci A, Kranenburg T, Grosveld G. Overexpression of N-Myc rapidly causes acute myeloid leukemia in mice. Cancer Res. 2007;67:10677-85 pubmed
	Cardone M, Kandilci A, Carella C, Nilsson J, Brennan J, Sirma S, et al. The novel ETS factor TEL2 cooperates with Myc in B lymphomagenesis. Mol Cell Biol. 2005;25:2395-405 pubmed

product information

Product Type :

Antibody

Product Name :

E2F1 Monoclonal Antibody (KH95)

Catalog # :

32-1400

Quantity :

100 µg

Price :

425 USD

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ChIP assay: 2 µg/10^6 cells, Immunohistochemistry: 0.1-1.0 µg/mL, Immunoprecipitation: 2-5 µg, Western Blot: 1-3 µg/mL

Species :

Human, Mouse, Rat

Clone :

KH95

Isotype :

IgG2a

Storage :

-20°C

Description :

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Immunogen :

Recombinant human E2F-1 protein.

Format :

Liquid

Applications w/Dilutions :

ChIP assay: 2 µg/10^6 cells, Immunohistochemistry: 0.1-1.0 µg/mL, Immunoprecipitation: 2-5 µg, Western Blot: 1-3 µg/mL

Aliases :

E2F transcription factor 1; E2f1; E2F-1; mKIAA4009; PBR3; PRB-binding protein E2F-1; RBAP1; RBAP-1; RBBP3; RBBP-3; RBP3; Retinoblastoma-associated protein 1; retinoblastoma-binding protein 3; Transcription factor E2F1

more info or order :

Invitrogen product webpage