Phospho-Histone H3 (Ser10) Polyclonal Antibody
Invitrogen
catalog: PA5-17869
domestic rabbit polyclonal
reactivity: human, mouse, rat, fruit fly , Xenopus laevis
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 29

Immunofluorescent analysis of Phospho-Histone H3 pSer10 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or a ToxInsight Instrument at 20X magnification.

Immunohistochemistry was performed on paraffin-embedded human breast carcinoma. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes at 95C. Following antigen-retrieval, tissues were incubated in 3% hydrogen peroxide for 10 minutes, washed, and then blocked in 5% normal goat serum in TBST for 1 hour at room temperature. Tissues were stained with a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:200 overnight at 4C. Tissues were washed extensively with TBS + 0.1% Tween-20 and detection was performed using a HRP-conjugated secondary reagent followed by DAB substrate.

Flow cytometric analysis of Phospho-Histone H3 pSer10 on Ramos cells left untreated (blue histogram) or treated with serum/calyculin (green histogram). Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-Histone H3 pSer10 polyclonal antibody (Product # PA5-17869) at a dilution of 1:50 for 1 hour at room temperature. As a negative control, serum/calyculin-treated cells were stained with an isotype control (red histogram). Following staining with either PA5-17869 or isotype control, cells were stained with a fluorescently-conjugated anti-rabbit IgG secondary antibody and analyzed on a flow cytometer.
quantity: 100 uL
price: US 514.00
to the supplier
H3K4me3 Monoclonal Antibody (G.532.8)
Invitrogen
catalog: MA5-11199
domestic rabbit monoclonal (G.532.8)
reactivity: Saccharomyces cerevisiae S288C, human, mouse, rat, fruit fly
application: western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 5
quantity: 100 uL
price: US 524.00
to the supplier
Histone H3 Monoclonal Antibody (E.960.2)
Invitrogen
catalog: MA5-15150
domestic rabbit monoclonal (E.960.2)
reactivity: human, mouse, rat, dogs, bovine, zebrafish , fruit fly
application: western blot
citations: 5
quantity: 100 uL
price: US 464.00
to the supplier
Histone H3 Polyclonal Antibody
Invitrogen
catalog: PA5-31954
domestic rabbit polyclonal
reactivity: human, mouse, rat, fruit fly
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 4

Immunohistochemical analysis of paraffin-embedded Hela xenograft using Histone H3 (PA5-31954) antibody at 1:500 dilution.

Western Blot analysis of Histone H3 using anti-Histone H3 Polyclonal Antibody (PA5-31954), in Jurkat Cell Lysate (30 ug of cell lysate) and at a dilution of 1:1000.
quantity: 100 uL
price: US 470.00
to the supplier
H3K9me2 Polyclonal Antibody
Invitrogen
catalog: PA5-16195
domestic rabbit polyclonal
reactivity: human, mouse, rat, fruit fly
application: western blot, immunocytochemistry, immunoprecipitation
citations: 2
quantity: 100 uL
price: US 454.00
to the supplier
H3K27me3 Polyclonal Antibody
Invitrogen
catalog: A15024
domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish , fruit fly
application: western blot, ELISA, immunocytochemistry, dot blot
citations: 2
quantity: 50 ug
price: US 450.00
to the supplier
H3K9ac Recombinant Rabbit Monoclonal Antibody (17H12L11)
Invitrogen
catalog: 701269
domestic rabbit recombinant (17H12L11)
reactivity: human, rat, fruit fly
application: western blot, immunocytochemistry
citations: 1
quantity: 100 ug
price: US 520.00
to the supplier
H3K9ac Recombinant Superclonal Antibody (17HCLC)
Invitrogen
catalog: 710293
domestic rabbit monoclonal (17HCLC)
reactivity: human, rat, fruit fly
application: western blot, immunocytochemistry
citations: 1
quantity: 100 ug
price: US 520.00
to the supplier
H3K9acS10ph Monoclonal Antibody (APH3-64)
Invitrogen
catalog: MA1-25461
mouse monoclonal (APH3-64)
reactivity: human, mouse, rat, chicken, bovine, fruit fly , roundworm
modification: phosphorylated
application: western blot, ELISA, immunoprecipitation
quantity: 50 uL
price: US 474.00
to the supplier
H3K9acS10ph Polyclonal Antibody
Invitrogen
catalog: PA1-25462
domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken, bovine, fruit fly , Xenopus laevis, roundworm
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 50 uL
price: US 464.00
to the supplier
Phospho-Histone H3 (Ser28) Polyclonal Antibody
Invitrogen
catalog: PA5-17318
domestic rabbit polyclonal
reactivity: human, mouse, fruit fly
modification: phosphorylated
application: western blot, immunocytochemistry, flow cytometry
quantity: 100 uL
price: US 514.00
to the supplier
H3K36me2 Monoclonal Antibody (GT1488)
Invitrogen
catalog: MA5-31513
mouse monoclonal (GT1488)
reactivity: human, fruit fly
application: western blot
quantity: 100 uL
price: US 474.00
to the supplier