NeutrAvidin Protein | Invitrogen 31000 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

protein

product name :

NeutrAvidin Protein

catalog :

31000

quantity :

10 mg

price :

US 165.00

citations: 15

Published Application/Species/Dilution	Reference
	Liba O, SoRelle E, Sen D, de la Zerda A. Contrast-enhanced optical coherence tomography with picomolar sensitivity for functional in vivo imaging. Sci Rep. 2016;6:23337 pubmed publisher
	Wang X, Rahil Z, Li I, Chowdhury F, Leckband D, Chemla Y, et al. Constructing modular and universal single molecule tension sensor using protein G to study mechano-sensitive receptors. Sci Rep. 2016;6:21584 pubmed publisher
	Yoshida K, Nagasaka T, Umeda Y, Tanaka T, Kimura K, Taniguchi F, et al. Expansion of epigenetic alterations in EFEMP1 promoter predicts malignant formation in pancreatobiliary intraductal papillary mucinous neoplasms. J Cancer Res Clin Oncol. 2016;142:1557-69 pubmed publisher
	Kim K, Kim D, Lee S, Kim S, Noh J, Kim J, et al. Pairwise detection of site-specific receptor phosphorylations using single-molecule blotting. Nat Commun. 2016;7:11107 pubmed publisher
	Gunawardane R, Fordstrom P, Piper D, Masterman S, Siu S, Liu D, et al. Agonistic Human Antibodies Binding to Lecithin-Cholesterol Acyltransferase Modulate High Density Lipoprotein Metabolism. J Biol Chem. 2016;291:2799-811 pubmed publisher
	Abdiche Y, Yeung Y, Chaparro-Riggers J, Barman I, Strop P, Chin S, et al. The neonatal Fc receptor (FcRn) binds independently to both sites of the IgG homodimer with identical affinity. MAbs. 2015;7:331-43 pubmed publisher
	Volpetti F, Garcia-Cordero J, Maerkl S. A microfluidic platform for high-throughput multiplexed protein quantitation. PLoS ONE. 2015;10:e0117744 pubmed publisher
	Valley C, Liu S, Lidke D, Lidke K. Sequential superresolution imaging of multiple targets using a single fluorophore. PLoS ONE. 2015;10:e0123941 pubmed publisher
	Schiele F, van Ryn J, Litzenburger T, Ritter M, Seeliger D, Nar H. Structure-guided residence time optimization of a dabigatran reversal agent. MAbs. 2015;7:871-80 pubmed publisher
	Chinen T, Liu P, Shioda S, Pagel J, Cerikan B, Lin T, et al. The γ-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycle. Nat Commun. 2015;6:8722 pubmed publisher
	Leulmi H, Socolovschi C, Laudisoit A, Houemenou G, Davoust B, Bitam I, et al. Detection of Rickettsia felis, Rickettsia typhi, Bartonella Species and Yersinia pestis in Fleas (Siphonaptera) from Africa. PLoS Negl Trop Dis. 2014;8:e3152 pubmed publisher
	Ni Z, Xu C, Guo X, Hunter G, Kuznetsova O, Tempel W, et al. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nat Struct Mol Biol. 2014;21:686-95 pubmed publisher
	Hecht R, Li Y, Sun J, Belouski E, Hall M, Hager T, et al. Rationale-Based Engineering of a Potent Long-Acting FGF21 Analog for the Treatment of Type 2 Diabetes. PLoS ONE. 2012;7:e49345 pubmed publisher
	Bee C, Abdiche Y, Pons J, Rajpal A. Determining the binding affinity of therapeutic monoclonal antibodies towards their native unpurified antigens in human serum. PLoS ONE. 2013;8:e80501 pubmed publisher
	Halpin D, Kalab P, Wang J, Weis K, Heald R. Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts. PLoS Biol. 2011;9:e1001225 pubmed publisher

product information

Product Type :

Protein

Product Name :

NeutrAvidin Protein

Catalog # :

31000

Quantity :

10 mg

Price :

US 165.00

Purity :

purified

Applications :

ELISA: Assay Dependent, Immunohistochemistry: Assay Dependent, In Situ Hybridization (ISH): Assay Dependent, Western Blot: Assay Dependent

Storage :

4° C

Description :

Thermo Scientific NeutrAvidin Protein is a specially-prepared form of avidin biotin-binding protein that decreases background in Western blotting and ELISA; available items include standard HRP, AP and fluorescent conjugates for biotin-based assays. NeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified and conjugated forms of NeutrAvidin Protein provide exceptional performance in Western blot, ELISA and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin. Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin-binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin binding proteins. The specific activity for biotin-binding is approximately 14µg/mg of protein, which is near the theoretical maximum activity.

Format :

Lyophilized

Applications w/Dilutions :

ELISA: Assay Dependent, Immunohistochemistry: Assay Dependent, In Situ Hybridization (ISH): Assay Dependent, Western Blot: Assay Dependent