TRA-1-60 Monoclonal Antibody (TRA-1-60)
Invitrogen
catalog: MA1-023X
mouse monoclonal (TRA-1-60)
reactivity: bovine
application: western blot, immunocytochemistry, flow cytometry
citations: 41

Immunofluorescent analysis of TRA-1-60 using anti-TRA-1-60 monoclonal antibody (Product# MA1-023) shows staining on the cell surface of human HEL 11.4 iPS cells, indicating pluripotency. TRA-1-60 staining (green) and an overlay image of TRA-1-60 with DAPI (blue) is shown. HEL 11.4 iPS cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a Monoclonal antibody recognizing TRA-1-60 (Product# MA1-023) at a dilution of 1:50 overnight at 4 ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken at 20X magnification.

Flow cytometry analysis of TRA-1-60 using anti-TRA-1-60 monoclonal antibody (Product# MA1-023) shows positive staining of H9 human embryonic stem cells (blue line). H9 human embryonic stem cells were harvested, fixed and washed with PBS. Cells were incubated with anti-TRA-1-60 monoclonal antibody (Product# MA1-023) or control at a 1:100 dilution for 1 hour on ice, followed by 30 min incubation with fluorescein conjugated secondary antibody. 100,000 cells were stained for each sample.

Western blot analysis of TRA-1-60 was performed by loading 100ug of NTERA-2 whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing TRA-1-60 (Product # MA1-023) at a dilution of 1:500 overnight at 4 ºC on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-IgM HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Dura (Product #34075).
quantity: 20 uL
price: US 177.00
to the supplier
GRP94 Monoclonal Antibody (9G10)
Invitrogen
catalog: MA3-016
rat monoclonal (9G10)
reactivity: Chinese hamsters, human, mouse, rat, chicken, bovine
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 24

Immunofluorescent analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) (Product# MA3-016 ) shows staining in C6 Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 (Product# MA3-016 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse breast tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Glucose Regulated Protein 94 (MA3-016) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100 uL
price: US 491.00
to the supplier
TRA-1-81 Monoclonal Antibody (TRA-1-81)
Invitrogen
catalog: MA1-024X
mouse monoclonal (TRA-1-81)
reactivity: bovine
application: western blot, immunocytochemistry, flow cytometry
citations: 16

Flow cytometry analysis of TRA-1-81 using anti-TRA-1-81 monoclonal antibody (Product # MA1-024) shows positive staining of HEL 11.4 iPS cells (blue line). HEL 11.4 iPS cells were harvested, fixed and washed with PBS. Cells were incubated with anti-TRA-1-81 monoclonal antibody (Product # MA1-024) or control at a 1:100 dilution for 1 hour on ice, followed by 30 min incubation with fluorescein conjugated secondary antibody. 100,000 cells were stained for each sample.

Immunofluorescent analysis of TRA-1-81 using anti-TRA-1-81 monoclonal antibody (Product # MA1-024) shows staining on the cell surface of human HEL 11.4 iPS cells, indicating pluripotency. TRA-1-81 staining (green) and an overlay image of TRA-1-81 with DAPI (blue) is shown. HEL 11.4 iPS cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a Monoclonal antibody recognizing TRA-1-81 (Product # MA1-024) at a dilution of 1:20 overnight at 4 ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken at 20X magnification.

Western blot analysis of TRA-1-81 was performed by loading 100ug of NTERA-2 whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing TRA-1-81 (Product # MA1-024) at a dilution of 1:1500 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-IgM HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).
quantity: 20 uL
price: US 177.00
to the supplier
GRP94 Monoclonal Antibody (9G10.F8.2)
Invitrogen
catalog: MA5-14592
rat monoclonal (9G10.F8.2)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, dogs, chicken, bovine, pigs , domestic sheep, Xenopus laevis, domestic rabbit, domestic horse
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 4
quantity: 500 uL
price: US 474.00
to the supplier
GRP94 Monoclonal Antibody (9G10), APC
Invitrogen
catalog: MA5-45096
rat monoclonal (9G10)
reactivity: Chinese hamsters, guinea pig, human, mouse, rat, dogs, chicken, bovine, pigs , domestic sheep, Xenopus laevis, domestic rabbit, domestic horse
conjugate: APC
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
quantity: 200 ug
price: US 454.00
to the supplier
GRP94 Monoclonal Antibody (9G10), FITC
Invitrogen
catalog: MA5-45097
rat monoclonal (9G10)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, dogs, chicken, bovine, pigs , domestic sheep, Xenopus laevis, domestic rabbit, domestic horse
conjugate: FITC
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
quantity: 200 ug
price: US 444.00
to the supplier
GRP94 Monoclonal Antibody (9G10), PerCP
Invitrogen
catalog: MA5-45098
rat monoclonal (9G10)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, dogs, chicken, bovine, pigs , domestic sheep, Xenopus laevis, domestic rabbit, domestic horse
conjugate: PerCP
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
quantity: 200 ug
price: US 454.00
to the supplier