HPV Type 16 E7 Monoclonal Antibody (8C9) | Invitrogen 28-0006 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HPV Type 16 E7 Monoclonal Antibody (8C9)

catalog :

28-0006

quantity :

50 µg

price :

411 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8C9

reactivity :

Human papillomavirus, mouse, Human papillomavirus type 16

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
western blot; Human papillomavirus; fig 1	Hobbs R, Batazzi A, Han M, Coulombe P. Loss of Keratin 17 induces tissue-specific cytokine polarization and cellular differentiation in HPV16-driven cervical tumorigenesis in vivo. Oncogene. 2016;35:5653-5662 pubmed publisher
immunoprecipitation; mouse; fig s1g western blot; mouse; fig s1g	Hobbs R, DePianto D, Jacob J, Han M, Chung B, Batazzi A, et al. Keratin-dependent regulation of Aire and gene expression in skin tumor keratinocytes. Nat Genet. 2015;47:933-8 pubmed publisher
immunoprecipitation; Human papillomavirus	Dichamp I, Seite P, Agius G, Barbarin A, Beby Defaux A. Human papillomavirus 16 oncoprotein E7 stimulates UBF1-mediated rDNA gene transcription, inhibiting a p53-independent activity of p14ARF. PLoS ONE. 2014;9:e96136 pubmed publisher
western blot; Human papillomavirus; 1:200	Pang C, Toh S, He P, Teissier S, Ben Khalifa Y, Xue Y, et al. A functional interaction of E7 with B-Myb-MuvB complex promotes acute cooperative transcriptional activation of both S- and M-phase genes. (129 c). Oncogene. 2014;33:4039-49 pubmed publisher
immunocytochemistry; Human papillomavirus type 16 western blot; Human papillomavirus type 16	Laurson J, Raj K. Localisation of human papillomavirus 16 E7 oncoprotein changes with cell confluence. PLoS ONE. 2011;6:e21501 pubmed publisher
immunocytochemistry; Human papillomavirus; 1:25; fig 4	Sulg M, Kirjavainen A, Pajusola K, Bueler H, Ylikoski J, Laiho M, et al. Differential sensitivity of the inner ear sensory cell populations to forced cell cycle re-entry and p53 induction. J Neurochem. 2010;112:1513-26 pubmed publisher
	Suksanpaisan L, Xu R, Tesfay M, Bomidi C, Hamm S, Vandergaast R, et al. Preclinical Development of Oncolytic Immunovirotherapy for Treatment of HPVPOS Cancers. Mol Ther Oncolytics. 2018;10:1-13 pubmed publisher
	Jiang R, Wang J, Peng S, Huang T, Wang C, Cannella F, et al. Spontaneous and Vaccine-Induced Clearance of Mus Musculus Papillomavirus 1 Infection. J Virol. 2017;91: pubmed publisher
	Kayama K, Watanabe S, Takafuji T, Tsuji T, Hironaka K, Matsumoto M, et al. GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis. EMBO Rep. 2017;18:123-137 pubmed publisher
	Strickland S, Vande Pol S. The Human Papillomavirus 16 E7 Oncoprotein Attenuates AKT Signaling To Promote Internal Ribosome Entry Site-Dependent Translation and Expression of c-MYC. J Virol. 2016;90:5611-5621 pubmed publisher
	Fan X, Liu Y, Heilman S, Chen J. Human papillomavirus E7 induces rereplication in response to DNA damage. J Virol. 2013;87:1200-10 pubmed publisher
	Grasso F, Negri D, Mochi S, Rossi A, Cesolini A, Giovannelli A, et al. Successful therapeutic vaccination with integrase defective lentiviral vector expressing nononcogenic human papillomavirus E7 protein. Int J Cancer. 2013;132:335-44 pubmed publisher
	Isaacson Wechsler E, Wang Q, Roberts I, Pagliarulo E, Jackson D, Untersperger C, et al. Reconstruction of human papillomavirus type 16-mediated early-stage neoplasia implicates E6/E7 deregulation and the loss of contact inhibition in neoplastic progression. J Virol. 2012;86:6358-64 pubmed publisher
	Bodily J, Mehta K, Cruz L, Meyers C, Laimins L. The E7 open reading frame acts in cis and in trans to mediate differentiation-dependent activities in the human papillomavirus type 16 life cycle. J Virol. 2011;85:8852-62 pubmed publisher
	Handa K, Yugawa T, Narisawa Saito M, Ohno S, Fujita M, Kiyono T. E6AP-dependent degradation of DLG4/PSD95 by high-risk human papillomavirus type 18 E6 protein. J Virol. 2007;81:1379-89 pubmed
	Berger K, Barriga F, Lace M, Turek L, Zamba G, Domann F, et al. Cervical keratinocytes containing stably replicating extrachromosomal HPV-16 are refractory to transformation by oncogenic H-Ras. Virology. 2006;356:68-78 pubmed
	Simonson S, Difilippantonio M, Lambert P. Two distinct activities contribute to human papillomavirus 16 E6's oncogenic potential. Cancer Res. 2005;65:8266-73 pubmed
	Zhao L, Samuels T, Winckler S, Korgaonkar C, Tompkins V, Horne M, et al. Cyclin G1 has growth inhibitory activity linked to the ARF-Mdm2-p53 and pRb tumor suppressor pathways. Mol Cancer Res. 2003;1:195-206 pubmed
	Debnath J, Mills K, Collins N, Reginato M, Muthuswamy S, Brugge J. The role of apoptosis in creating and maintaining luminal space within normal and oncogene-expressing mammary acini. Cell. 2002;111:29-40 pubmed
	Thompson D, Zacny V, Belinsky G, Classon M, Jones D, Schlegel R, et al. The HPV E7 oncoprotein inhibits tumor necrosis factor alpha-mediated apoptosis in normal human fibroblasts. Oncogene. 2001;20:3629-40 pubmed
	Loeuillet C, Douay L, Herve P, Chalmers D. Preservation of the myofibroblastic phenotype of human papilloma virus 16 E6/E7 immortalized human bone marrow cells using the lineage limited alpha-smooth muscle actin promoter. Cell Growth Differ. 2001;12:233-42 pubmed

product information

Product Type :

Antibody

Product Name :

HPV Type 16 E7 Monoclonal Antibody (8C9)

Catalog # :

28-0006

Quantity :

50 µg

Price :

411 USD

Clonality :

Monoclonal

Purity :

HPLC

Host :

Mouse

Reactivity :

Virus

Applications :

Immunohistochemistry (Paraffin): 1:2, Immunoprecipitation: 1:10, Western Blot: 1:100

Species :

Virus

Clone :

8C9

Isotype :

IgG1

Storage :

4° C

Description :

The HPV E7 proteins are small (HPV16 E7 comprising 98 amino acids, 15kDa), zinc binding phosphoproteins which are localised in the nucleus. They are structurally and functionally similar to the E1A protein of subgenus C adenoviruses. The first 16 amino-terminal amino acids of HPV16 E7 contain a region homologous to a segment of the conserved region 1 (CR1) of the E1A protein of subgenus C adenoviruses. The next domain, up to amino acid 37, is homologous to the entire region 2 (CR2) of E1A. Genetic studies have established that these domains are required for cell transformation in vitro, suggesting similarities in the mechanism of transformation by these viruses. The CR2 homology region contains the LXCXE motif (residues 22-26) involved in binding to the tumour suppressor protein pRb.

Immunogen :

Bacterially derived fusion protein containing the human papillomavirus tp 16 early protein E7 open reading frame.

Format :

Lyophilized

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:2, Immunoprecipitation: 1:10, Western Blot: 1:100

Aliases :

HPV 16-E7; HPV16E7; human papillomavirus

more info or order :

Invitrogen product webpage