GLO1 Monoclonal Antibody (Glo1a)
Invitrogen
catalog: MA1-13029

mouse monoclonal (Glo1a)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 7

Western blot analysis of Glyoxylase (Glo1) was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a Mouse monoclonal antibody recognizing Glyoxylase (Product # MA1-13029) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a Goat anti-Mouse-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product #34077). For siRNA knock-down experiments HeLa and U20S cells were treated with 100nM Glo1 siRNA or control siRNA for 48 hours. Lysates were collected and Western blot analysis performed as described above.

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human Prostate and Stomach cancer tissues. To expose target proteins high pressure heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes. Following antigen retrieval endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 minutes at room-temp. Tissues were then washed in PBS and blocked in 10% normal goat serum for 20 minutes at room temperature. Tissues were probed at a dilution of 1:800 with a Mouse monoclonal antibody recognizing Glyoxylase (Product # MA1-13029) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBS. Colorimetric detection was performed using metal enhanced DAB and tissues counterstained with hematoxylin. Images are displayed at 40X magnification. Results demonstrate cytoplasmic localization of Glyoxylase.
quantity: 100 ug
price: US 491.00
to the supplier

GLO1 Monoclonal Antibody (Glo1a)
Invitrogen
catalog: MA1-13029X

mouse monoclonal (Glo1a)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 7

Western blot analysis of Glyoxylase (Glo1) was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a Mouse monoclonal antibody recognizing Glyoxylase (Product # MA1-13029) at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a Goat anti-Mouse-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Pico (Product #34077). For siRNA knock-down experiments HeLa and U20S cells were treated with 100nM Glo1 siRNA or control siRNA for 48 hours. Lysates were collected and Western blot analysis performed as described above.
quantity: 20 uL
price: US 177.00
to the supplier

GLO1 Polyclonal Antibody
Invitrogen
catalog: PA5-44216

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity: 100 uL
price: US 514.00
to the supplier

GLO1 Polyclonal Antibody
Invitrogen
catalog: PA5-77987

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 522.00
to the supplier

GLO1 Polyclonal Antibody
Invitrogen
catalog: PA5-79315

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 100 ug
price: US 434.00
to the supplier

GLO1 Monoclonal Antibody (GT266)
Invitrogen
catalog: MA5-31468

mouse monoclonal (GT266)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

GLO1 Monoclonal Antibody (GT552)
Invitrogen
catalog: MA5-31469

mouse monoclonal (GT552)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

GLO1 Polyclonal Antibody
Invitrogen
catalog: PA5-84436

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

quantity: 100 uL
price: US 474.00
to the supplier

GLO1 Polyclonal Antibody
Invitrogen
catalog: PA5-95997

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

GLO1 Recombinant Rabbit Monoclonal Antibody (JU44-11)
Invitrogen
catalog: MA5-34623

domestic rabbit recombinant (JU44-11)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

GLO1 Recombinant Rabbit Monoclonal Antibody (ARC0969)
Invitrogen
catalog: MA5-35481

domestic rabbit recombinant (ARC0969)
reactivity: human, mouse, rat
application: western blot, ELISA

quantity: 100 uL
price: US 474.00
to the supplier

GLO1 Polyclonal Antibody
Invitrogen
catalog: PA5-115287

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

GLO1 Recombinant Rabbit Monoclonal Antibody (23GB5555)
Invitrogen
catalog: MA5-53898

domestic rabbit recombinant (23GB5555)
reactivity: human, mouse, rat
application: western blot, flow cytometry

quantity: 100 uL
price: US 539.00
to the supplier

GLO1 Recombinant Rabbit Monoclonal Antibody (23GB5555)
Invitrogen
catalog: MA5-53899

domestic rabbit recombinant (23GB5555)
reactivity: human, mouse, rat
application: western blot, flow cytometry

quantity: 20 uL
price: US 199.00
to the supplier