ERK1/ERK2 Monoclonal Antibody (ERK-7D8)
Invitrogen
catalog: 13-6200
mouse monoclonal (ERK-7D8)
reactivity: human, mouse, rat, dog, common platanna
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 36
quantity: 200µg
price: US 345
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat, chicken, cow, common platanna, Caenorhabditis elegans
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 40
quantity: 100µL
price: US 345
to the supplier
ERK1/ERK2 Polyclonal Antibody
Invitrogen
catalog: 44-654G
rabbit polyclonal
reactivity: human, mouse, rat, cow
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 22
quantity: 100µL
price: US 345
to the supplier
mouse monoclonal (MILAN8R)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 12
quantity: 25µg
price: US 150
to the supplier
mouse monoclonal (MILAN8R)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 12
quantity: 100µg
price: US 322
to the supplier
ERK1/ERK2 Polyclonal Antibody
Invitrogen
catalog: 61-7400
rabbit polyclonal
reactivity: African green monkey, human, mouse, rat, rabbit
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 15
quantity: 100µg
price: US 345
to the supplier
ERK1 Monoclonal Antibody (ERK-6B11)
Invitrogen
catalog: 13-8600
mouse monoclonal (ERK-6B11)
reactivity: human, mouse, rat, dog
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 10
quantity: 100µg
price: US 345
to the supplier
mouse monoclonal (MILAN8R)
reactivity: human, mouse
conjugate: APC
application: flow cytometry
citations: 5
quantity: 25Tests
price: US 130
to the supplier
mouse monoclonal (MILAN8R)
reactivity: human, mouse
conjugate: APC
application: flow cytometry
citations: 4
quantity: 100Tests
price: US 336
to the supplier
mouse monoclonal (5AD13MA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 6
quantity: 25µg
price: US 127
to the supplier
mouse monoclonal (5AD13MA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 5
quantity: 100µg
price: US 305
to the supplier
mouse monoclonal (MILAN8R)
reactivity: human, mouse
conjugate: Percp/efluor 710
application: flow cytometry
citations: 2
quantity: 25Tests
price: US 138
to the supplier
mouse monoclonal (MILAN8R)
reactivity: human, mouse
conjugate: Percp/efluor 710
application: flow cytometry
citations: 2
quantity: 100Tests
price: US 332
to the supplier
mouse monoclonal (MILAN8R)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
citations: 2
quantity: 25Tests
price: US 139
to the supplier
mouse monoclonal (MILAN8R)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
citations: 2
quantity: 100Tests
price: US 338
to the supplier
mouse monoclonal (MILAN8R)
reactivity: human, mouse
conjugate: PE-eFluor 610
application: flow cytometry
citations: 2
quantity: 25Tests
price: US 151
to the supplier
mouse monoclonal (MILAN8R)
reactivity: human, mouse
conjugate: PE-eFluor 610
application: flow cytometry
citations: 2
quantity: 100Tests
price: US 371
to the supplier
mouse monoclonal (MILAN8R)
reactivity: human, mouse
conjugate: AF488
application: flow cytometry
citations: 3
quantity: 25Tests
price: US 139
to the supplier
mouse monoclonal (MILAN8R)
reactivity: human, mouse
conjugate: AF488
application: flow cytometry
citations: 3
quantity: 100Tests
price: US 331
to the supplier
ERK1/ERK2 Polyclonal Antibody
Invitrogen
catalog: 82380
rabbit polyclonal
reactivity: Chinese hamster, human, mouse, rat, cow, pig, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 1
ERK1/ERK2 Monoclonal Antibody (K.913.4)
Invitrogen
catalog: MA5-15134
rabbit monoclonal (K.913.4)
reactivity: Chinese hamster, human, mouse, rat, dog, chicken, cow, pig, zebrafish , fruit fly, Caenorhabditis elegans
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 1
quantity: 200µL
price: US 345
to the supplier
rabbit monoclonal (S.812.9)
reactivity: Chinese hamster, human, mouse, rat, pig, zebrafish , fruit fly
modification: phosphorylated
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 1

quantity: 200µL
price: US 345
to the supplier
rabbit monoclonal (B.742.5)
reactivity: Stylophora pistillata, human, mouse, rat, pig, zebrafish , fruit fly
modification: phosphorylated
application: western blot, immunocytochemistry, flow cytometry
citations: 1
quantity: 200µL
price: US 345
to the supplier
ERK1 Monoclonal Antibody (12D11)
Invitrogen
catalog: MA1-13041
mouse monoclonal (12D11)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1

Western blot analysis of ERK1 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing ERK1 (Product # MA1-13041) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 32430) at a dilution of 1:25,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product #34077).

Immunofluorescent analysis of ERK1 (green) in untreated and siRNA knockdown treated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing ERK1 (Product # MA1-13041), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 549 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Immunohistochemistry was performed on cancer biopsies of deparaffinized human colon tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95°C. Following antigen retrieval tissues were blocked in 10% normal goat serum (Product #31873)/TBS (Product #28376) for two hours at room temperature. Tissues were probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing ERK1 (Product # MA1-13041) overnight at 4°C in a humidified chamber. Tissues were washed extensively with TBS/0.025% Triton (Product #28314) and endogenous peroxidase activity quenched with Peroxidase Suppressor (Product #35000) for 30 minutes at room temperature. Detection was performed using a goat anti-mouse HRP secondary antibody (Product #31430) at a dilution of 1:500 followed by colorimetric detection using metal enhanced DAB (Product #34065). Images were taken on a Zeiss Axiovision microscope at 10X magnification. Results demonstrate strong cytoplasmic localization of ERK1.
quantity: 100µL
price: US 390
to the supplier
ERK1/ERK2 Monoclonal Antibody (3F8B3)
Invitrogen
catalog: MA5-15605
mouse monoclonal (3F8B3)
reactivity: Stylophora pistillata, human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1
mouse monoclonal (5AD13MA)
reactivity: human, mouse, rat
conjugate: eFluor 570
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1
quantity: 100µg
price: US 304
to the supplier
ERK1/ERK2 Monoclonal Antibody (3A1)
Invitrogen
catalog: LF-MA0178
mouse monoclonal (3A1)
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100µL
price: US 345
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot
quantity: 100µL
price: US 345
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry
quantity: 30µL
price: US 390
to the supplier
ERK1/ERK2 Monoclonal Antibody (E.657.8)
Invitrogen
catalog: MA5-15227
mouse monoclonal (E.657.8)
reactivity: Chinese hamster, human, mouse, rat, cow, pig, zebrafish
application: western blot
quantity: 100µL
price: US 345
to the supplier
ERK1 Monoclonal Antibody (1E5)
Invitrogen
catalog: MA5-15896
mouse monoclonal (1E5)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
quantity: 100µL
price: US 345
to the supplier
rabbit monoclonal (6HCLC)
reactivity: human, mouse
application: western blot
quantity: 100µg
price: US 345
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
conjugate: AF488
modification: phosphorylated
application: immunocytochemistry
quantity: 100µL
price: US 345
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100µl
price: US 390
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100µl
price: US 390
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100µl
price: US 345
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100µl
price: US 345
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100µl
price: US 345
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100µl
price: US 345
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100µl
price: US 345
to the supplier
ERK1 Polyclonal Antibody
Invitrogen
catalog: PA5-77818
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
quantity: 100µL
price: US 390
to the supplier
rabbit recombinant (ERK12T202Y204-A11)
reactivity: human, mouse
conjugate: APC
application: flow cytometry
quantity: 100 tests
price: US 347
to the supplier
rabbit recombinant (ERK12T202Y204-A11)
reactivity: human, mouse
conjugate: FITC
application: flow cytometry
quantity: 100 tests
price: US 337
to the supplier
rabbit recombinant (SC58-01)
reactivity: human, mouse, rat
application: western blot
quantity: 100µL
price: US 345
to the supplier
ERK1/ERK2 Polyclonal Antibody
Invitrogen
catalog: PA5-94937
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100µg
price: US 345
to the supplier
ERK1 Polyclonal Antibody
Invitrogen
catalog: PA5-94966
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100µg
price: US 345
to the supplier
Phospho-ERK1 (Tyr204) Polyclonal Antibody
Invitrogen
catalog: PA5-97359
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
quantity: 100µL
price: US 345
to the supplier