Invitrogen Ccnb1 antibody

Cyclin B1 Monoclonal Antibody (GNS11)
Invitrogen
catalog: MA5-14327

mouse monoclonal (GNS11)
reactivity: Chinese hamsters, human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 12

Immunofluorescent analysis of Cyclin B1 (green) in HeLa cells either left untreated (left panel) or treated with 50nM Nocodazole (right panel) for 16 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Cyclin B1 monoclonal antibody (Product # MA5-14327) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.

Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human tonsil tissue. To expose target proteins, heat-induced antigen retrieval was performed by boiling tissue sections in 10mM sodium citrate buffer (pH 6) for 10-20 minutes. Tissues were probed with a Cyclin B1 monoclonal antibody (Product # MA5-14327) at a concentration of 3ug/ml. Detection was performed using an anti-mouse IgG-HRP secondary antibody followed by a colorimetric substrate

Immunoprecipitation of Cyclin B1 was performed on the Human Colon Carcinoma cell line LS174T. Antigen-antibody complexes were formed by incubating 500ul of LS174T whole cell lysate with 5ul of a Cyclin B1 monoclonal antibody (Product # MA5-14327). The immune complexes were captured using Protein A agarose, washed extensively, and eluted. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a membrane, and probed with a series of reagents to detect Cyclin B1
quantity: 500 uL
price: US 524.00
to the supplier

Cyclin B1 Monoclonal Antibody (V152)
Invitrogen
catalog: MA1-155

mouse monoclonal (V152)
reactivity: Chinese hamsters, African green monkey, human, mouse, rat, pigs
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 4

quantity: 100 ug
price: US 491.00
to the supplier

Phospho-Cyclin B1 (Ser147) Polyclonal Antibody
Invitrogen
catalog: PA5-17682

domestic rabbit polyclonal
reactivity: human, mouse, rat, pigs
modification: phosphorylated
application: western blot

quantity: 100 uL
price: US 464.00
to the supplier

Cyclin B1 Monoclonal Antibody (1B10)
Invitrogen
catalog: MA5-15706

mouse monoclonal (1B10)
reactivity: human, rat
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 491.00
to the supplier

Cyclin B1 Monoclonal Antibody (5G6)
Invitrogen
catalog: MA5-15714

mouse monoclonal (5G6)
reactivity: human, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry

Immunofluorescence analysis of Hela cells using CCNB1 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
quantity: 100 uL
price: US 491.00
to the supplier

Phospho-Cyclin B1 (Ser128) Polyclonal Antibody
Invitrogen
catalog: PA5-64671

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

Phospho-Cyclin B1 (Ser133) Polyclonal Antibody
Invitrogen
catalog: PA5-64672

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

quantity: 100 uL
price: US 464.00
to the supplier

Cyclin B1 Polyclonal Antibody
Invitrogen
catalog: PA5-86223