Phosphotyrosine Monoclonal Antibody (pY20)
Invitrogen
catalog: MA1-82787

mouse monoclonal (PY20)
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 93

quantity: 500 ug
price: US 393.00
to the supplier

Phosphotyrosine Monoclonal Antibody (pY20)
Invitrogen
catalog: AHO0681

mouse monoclonal (PY20)
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 84

quantity: 100 ug
price: US 420.00
to the supplier

Phosphotyrosine Monoclonal Antibody (pY20)
Invitrogen
catalog: 03-7799

mouse monoclonal (PY20)
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 89

quantity: 1 mg
price: US 520.00
to the supplier

Phosphotyrosine Monoclonal Antibody (pY20)
Invitrogen
catalog: 03-7700

mouse monoclonal (PY20)
reactivity: silk moth
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 82

quantity: 100 ug
price: US 366.00
to the supplier

Phosphotyrosine Monoclonal Antibody (pY20), HRP
Invitrogen
catalog: MA1-12445

mouse monoclonal (PY20)
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
citations: 16

quantity: 50 ug
price: US 292.00
to the supplier

Phosphotyrosine Monoclonal Antibody (pY20), FITC
Invitrogen
catalog: MA1-12441

mouse monoclonal (PY20)
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
citations: 10

quantity: 100 ug
price: US 464.00
to the supplier

phospho-Tyrosine Monoclonal Antibody (pY20), eBioscience
Invitrogen
catalog: 14-5001-82

mouse monoclonal (PY20)
application: western blot, immunoprecipitation, flow cytometry, other
citations: 12

quantity: 100 ug
price: US 135.00
to the supplier

Phosphotyrosine Monoclonal Antibody (pY20), Biotin
Invitrogen
catalog: MA1-12439

mouse monoclonal (PY20)
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 11

quantity: 100 ug
price: US 474.00
to the supplier

Phosphotyrosine Polyclonal Antibody
Invitrogen
catalog: 61-5800

domestic rabbit polyclonal
application: western blot, ELISA, immunoprecipitation
citations: 11

quantity: 100 ug
price: US 451.00
to the supplier

Phosphotyrosine Monoclonal Antibody (pY20), AP
Invitrogen
catalog: 03-7722

mouse monoclonal (PY20)
conjugate: AP
application: western blot, ELISA
citations: 8

quantity: 100 ug
price: US 451.00
to the supplier

Phosphotyrosine Monoclonal Antibody (pY20), HRP
Invitrogen
catalog: 03-7720

mouse monoclonal (PY20)
conjugate: HRP
application: western blot, ELISA, immunocytochemistry
citations: 10

quantity: 100 ug
price: US 451.00
to the supplier

Phosphotyrosine Monoclonal Antibody (IG2)
Invitrogen
catalog: MA4-003

mouse monoclonal (IG2)
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 4

Immunofluorescent analysis of Phosphotyrosine (p-Tyr, green) in HeLa cells. Cells were probed with a p-Tyr monoclonal antibody (Product # MA4-003) followed by a fluorescently-conjugated anti-mouse IgG secondary antibody.

Western blot analysis of tyrosine phosphorylated c-Abl and Bcr-Abl was performed following immunoprecipitation of K562 cell lysates using a c-Abl antibody, from untreated cells (left lane) or cells treated with 10uM of the tyrosine kinase inhibitor Gleevec (right lane) for 24 hours. The captured immune complexes were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a Phosphotyrosine monoclonal antibody (Product # MA4-003) at a dilution of 1:250 overnight rotating at 4°C, washed in TBST, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

Immunoprecipitation of Phosphotyrosine (p-Tyr) was performed on K562 cell lysates from untreated cells (left lane) or from cells treated with 10uM of the tyrosine kinase inhibitor Gleevec (right lane) for 24 hours. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 2ug of a p-Tyr monoclonal antibody (Product # MA4-003) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a c-Abl polyclonal antibody at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
quantity: 100 ug
price: US 451.00
to the supplier

phospho-Tyrosine Monoclonal Antibody (pY20), PerCP-eFluor 710, eBioscience
Invitrogen
catalog: 46-5001-42

mouse monoclonal (PY20)
conjugate: Percp/efluor 710
application: flow cytometry
citations: 7

quantity: 100 Tests
price: US 475.00
to the supplier

phospho-Tyrosine Monoclonal Antibody (pY20), eFluor 450, eBioscience
Invitrogen
catalog: 48-5001-42

mouse monoclonal (PY20)
conjugate: eFluor 450
application: flow cytometry
citations: 7

quantity: 100 Tests
price: US 408.00
to the supplier

phospho-Tyrosine Monoclonal Antibody (pY20), eFluor 660, eBioscience
Invitrogen
catalog: 50-5001-42

mouse monoclonal (PY20)
conjugate: efluor 660
application: flow cytometry
citations: 7

quantity: 100 Tests
price: US 412.00
to the supplier

Phosphotyrosine Monoclonal Antibody (P-Tyr-01)
Invitrogen
catalog: MA1-10443

mouse monoclonal (P-TYR-01)
application: western blot, immunocytochemistry, flow cytometry
citations: 1

quantity: 100 ug
price: US 414.00
to the supplier

Phosphotyrosine Polyclonal Antibody, HRP
Invitrogen
catalog: 61-5820

domestic rabbit polyclonal
conjugate: HRP
application: western blot, ELISA

quantity: 100 ug
price: US 520.00
to the supplier

Phosphotyrosine Monoclonal Antibody (P-Tyr-01), Alexa Fluor 647
Invitrogen
catalog: MA5-18172

mouse monoclonal (P-TYR-01)
conjugate: AF647
application: western blot, immunocytochemistry, flow cytometry

quantity: 100 ug
price: US 369.00
to the supplier

Phosphotyrosine Recombinant Rabbit Monoclonal Antibody (RM111)
Invitrogen
catalog: MA5-27977

domestic rabbit recombinant (RM111)
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 491.00
to the supplier