This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD284 (TLR4) Monoclonal Antibody (HTA125), PE-Cyanine7, eBioscience
catalog :
25-9917-42
quantity :
100 Tests
price :
US 441.00
clonality :
monoclonal
host :
mouse
conjugate :
PE/Cy7
clone name :
HTA125
The same clone is also sold as:
The same clone is also sold as:
- BD Biosciences: 551964, 562256, 551975
- Abcam: ab30667, ab155343, ab8376, ab171260
- OriGene: TA320484, SM1677PS, SM1677P, SM1677B, SM1677LE, SM1677FT, SM1677F, SM1677R
- Novus Biologicals: NB100-56723, NB100-56727
- Santa Cruz Biotechnology: sc-13593 AC, sc-13593, sc-13593 L, sc-13593 HRP
- BioLegend: 312817, 312816, 312804, 312805, 312815, 312813, 312806, 312811, 312814, 312802, 312821, 312819
- Bio-Rad: MCA2061SBV570, MCA2061B, MCA2061A647, MCA2061A647T, MCA2061SBUV795, MCA2061SBV710, MCA2061SBV515, MCA2061SBV670, MCA2061SBUV510, MCA2061SBUV445, MCA2061SBV760, MCA2061SBUV665, MCA2061SBUV400, MCA2061SBB700, MCA2061SBV790, MCA2061SBUV605, MCA2061SBUV575, MCA2061, MCA2061PE, MCA2061SBUV740, MCA2061SBV440, MCA2061FT, MCA2061EL, MCA2061SBV610, MCA2061GA, MCA2061F, MCA2061SBV475
reactivity :
human
application :
flow cytometry
citations: 225
Reference |
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Velegraki M, Papakonstanti E, Mavroudi I, Psyllaki M, Tsatsanis C, Oulas A, et al. Impaired clearance of apoptotic cells leads to HMGB1 release in the bone marrow of patients with myelodysplastic syndromes and induces TLR4-mediated cytokine production. Haematologica. 2013;98:1206-15 pubmed publisher
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Huang X, Yi J, Yin S, Chen R, Li M, Gong Z, et al. Exposure to heat-inactivated Trichophyton rubrum resulting in a limited immune response of human keratinocytes. Chin Med J (Engl). 2013;126:215-9 pubmed
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Yoshino H, Kashiwakura I. Impairment of mature dendritic cells derived from X-irradiated human monocytes depends on the type of maturation stimulus used. Radiat Res. 2012;178:280-8 pubmed
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Nair A, Kanda V, Bush Joseph C, Verma N, Chubinskaya S, Mikecz K, et al. Synovial fluid from patients with early osteoarthritis modulates fibroblast-like synoviocyte responses to toll-like receptor 4 and toll-like receptor 2 ligands via soluble CD14. Arthritis Rheum. 2012;64:2268-77 pubmed publisher
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Caras I, Tucureanu C, Pitica R, Salageanu A. Bacterial extract cantastim activates macrophages via TLR-2. Roum Arch Microbiol Immunol. 2011;70:28-36 pubmed
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Scian R, Barrionuevo P, Giambartolomei G, Fossati C, Baldi P, Delpino M. Granulocyte-macrophage colony-stimulating factor- and tumor necrosis factor alpha-mediated matrix metalloproteinase production by human osteoblasts and monocytes after infection with Brucella abortus. Infect Immun. 2011;79:192-202 pubmed publisher
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Gomes N, Brunialti M, Mendes M, Freudenberg M, Galanos C, Salomao R. Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood. Braz J Med Biol Res. 2010;43:853-8 pubmed
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Soylu A, Ates H, Cingoz S, Turkmen M, Demir B, Tunca M, et al. TLR polymorphisms in FMF: association of TLR-2 (Arg753Gln) and TLR-4 (Asp299Gly, Thre399Ile) polymorphisms and myeloid cell TLR-2 and TLR-4 expression with the development of secondary amyloidosis in FMF. Inflammation. 2011;34:379-87 pubmed publisher
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Ahmad U, Ali R, Lebastchi A, Qin L, Lo S, Yakimov A, et al. IFN-gamma primes intact human coronary arteries and cultured coronary smooth muscle cells to double-stranded RNA- and self-RNA-induced inflammatory responses by upregulating TLR3 and melanoma differentiation-associated gene 5. J Immunol. 2010;185:1283-94 pubmed publisher
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Parker L, Prestwich E, Ward J, Smythe E, Berry A, Triantafilou M, et al. A phosphatidylserine species inhibits a range of TLR- but not IL-1beta-induced inflammatory responses by disruption of membrane microdomains. J Immunol. 2008;181:5606-17 pubmed
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Krämer S, Sellge G, Lorentz A, Krueger D, Schemann M, Feilhauer K, et al. Selective activation of human intestinal mast cells by Escherichia coli hemolysin. J Immunol. 2008;181:1438-45 pubmed
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Henning L, Azad A, Parsa K, Crowther J, Tridandapani S, Schlesinger L. Pulmonary surfactant protein A regulates TLR expression and activity in human macrophages. J Immunol. 2008;180:7847-58 pubmed
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Zhang Q, Qian F, Liu H, Zhou L, Huang M, Zhang X, et al. Expression of surface markers on peripheral CD4+CD25high T cells in patients with atopic asthma: role of inhaled corticosteroid. Chin Med J (Engl). 2008;121:205-12 pubmed
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Davey M, Liu X, Ukai T, Jain V, Gudino C, Gibson F, et al. Bacterial fimbriae stimulate proinflammatory activation in the endothelium through distinct TLRs. J Immunol. 2008;180:2187-95 pubmed
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Ibeagha Awemu E, Lee J, Ibeagha A, Bannerman D, Paape M, Zhao X. Bacterial lipopolysaccharide induces increased expression of toll-like receptor (TLR) 4 and downstream TLR signaling molecules in bovine mammary epithelial cells. Vet Res. 2008;39:11 pubmed
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Maratheftis C, Giannouli S, Spachidou M, Panayotou G, Voulgarelis M. RNA interference of interferon regulatory factor-1 gene expression in THP-1 cell line leads to Toll-like receptor-4 overexpression/activation as well as up-modulation of annexin-II. Neoplasia. 2007;9:1012-20 pubmed
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Méndez Samperio P, Belmont L, Miranda E. Mycobacterium bovis BCG Toll-like receptors 2 and 4 cooperation increases the innate epithelial immune response. Arch Med Res. 2008;39:33-9 pubmed
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Voelcker V, Gebhardt C, Averbeck M, Saalbach A, Wolf V, Weih F, et al. Hyaluronan fragments induce cytokine and metalloprotease upregulation in human melanoma cells in part by signalling via TLR4. Exp Dermatol. 2008;17:100-7 pubmed
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Udan M, Ajit D, Crouse N, Nichols M. Toll-like receptors 2 and 4 mediate Abeta(1-42) activation of the innate immune response in a human monocytic cell line. J Neurochem. 2008;104:524-33 pubmed
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Barrionuevo P, Cassataro J, Delpino M, Zwerdling A, Pasquevich K, García Samartino C, et al. Brucella abortus inhibits major histocompatibility complex class II expression and antigen processing through interleukin-6 secretion via Toll-like receptor 2. Infect Immun. 2008;76:250-62 pubmed
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Mandron M, Ariès M, Boralevi F, Martin H, Charveron M, Taieb A, et al. Age-related differences in sensitivity of peripheral blood monocytes to lipopolysaccharide and Staphylococcus aureus toxin B in atopic dermatitis. J Invest Dermatol. 2008;128:882-9 pubmed
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Dunn Siegrist I, Leger O, Daubeuf B, Poitevin Y, Dépis F, Herren S, et al. Pivotal involvement of Fcgamma receptor IIA in the neutralization of lipopolysaccharide signaling via a potent novel anti-TLR4 monoclonal antibody 15C1. J Biol Chem. 2007;282:34817-27 pubmed
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Loharungsikul S, Troye Blomberg M, Amoudruz P, Pichyangkul S, Yongvanitchit K, Looareesuwan S, et al. Expression of toll-like receptors on antigen-presenting cells in patients with falciparum malaria. Acta Trop. 2008;105:10-5 pubmed
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McCully M, Baroja M, Chau T, Jain A, Barra L, Salgado A, et al. Receptor-interacting protein 2 is a marker for resolution of peritoneal dialysis-associated peritonitis. Kidney Int. 2007;72:1273-81 pubmed
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Kobayashi R, Okamura S, Ohno T, Saito H, Mori M, Ra C, et al. Hyperexpression of FcgammaRI and Toll-like receptor 4 in the intestinal mast cells of Crohn's disease patients. Clin Immunol. 2007;125:149-58 pubmed
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Yerkovich S, Wikstrom M, Suriyaarachchi D, Prescott S, Upham J, Holt P. Postnatal development of monocyte cytokine responses to bacterial lipopolysaccharide. Pediatr Res. 2007;62:547-52 pubmed
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Kettner S, Kalthoff F, Graf P, Priller E, Kricek F, Lindley I, et al. EWI-2/CD316 is an inducible receptor of HSPA8 on human dendritic cells. Mol Cell Biol. 2007;27:7718-26 pubmed
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Schierloh P, Yokobori N, Aleman M, Landoni V, Geffner L, Musella R, et al. Mycobacterium tuberculosis-induced gamma interferon production by natural killer cells requires cross talk with antigen-presenting cells involving Toll-like receptors 2 and 4 and the mannose receptor in tuberculous pleurisy. Infect Immun. 2007;75:5325-37 pubmed
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Balducci E, Micossi L, Soldaini E, Rappuoli R. Expression and selective up-regulation of toxin-related mono ADP-ribosyltransferases by pathogen-associated molecular patterns in alveolar epithelial cells. FEBS Lett. 2007;581:4199-204 pubmed
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Haugen F, Drevon C. Activation of nuclear factor-kappaB by high molecular weight and globular adiponectin. Endocrinology. 2007;148:5478-86 pubmed
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Hasannejad H, Takahashi R, Kimishima M, Hayakawa K, Shiohara T. Selective impairment of Toll-like receptor 2-mediated proinflammatory cytokine production by monocytes from patients with atopic dermatitis. J Allergy Clin Immunol. 2007;120:69-75 pubmed
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Bonnefont Rebeix C, Marchal T, Bernaud J, Pin J, Leroux C, Lebecque S, et al. Toll-like receptor 3 (TLR3): a new marker of canine monocytes-derived dendritic cells (cMo-DC). Vet Immunol Immunopathol. 2007;118:134-9 pubmed
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Honda Y, Yamagiwa S, Matsuda Y, Takamura M, Ichida T, Aoyagi Y. Altered expression of TLR homolog RP105 on monocytes hypersensitive to LPS in patients with primary biliary cirrhosis. J Hepatol. 2007;47:404-11 pubmed
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Uehara A, Fujimoto Y, Fukase K, Takada H. Various human epithelial cells express functional Toll-like receptors, NOD1 and NOD2 to produce anti-microbial peptides, but not proinflammatory cytokines. Mol Immunol. 2007;44:3100-11 pubmed
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Chang J, Hampartzoumian T, Everett B, Lloyd A, McCluskey P, Wakefield D. Changes in Toll-like receptor (TLR)-2 and TLR4 expression and function but not polymorphisms are associated with acute anterior uveitis. Invest Ophthalmol Vis Sci. 2007;48:1711-7 pubmed
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Maratheftis C, Andreakos E, Moutsopoulos H, Voulgarelis M. Toll-like receptor-4 is up-regulated in hematopoietic progenitor cells and contributes to increased apoptosis in myelodysplastic syndromes. Clin Cancer Res. 2007;13:1154-60 pubmed
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Fernandez S, Palmer D, Simmons M, Sun P, Bisbing J, McClain S, et al. Potential role for Toll-like receptor 4 in mediating Escherichia coli maltose-binding protein activation of dendritic cells. Infect Immun. 2007;75:1359-63 pubmed
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Visvanathan K, Skinner N, Thompson A, Riordan S, Sozzi V, Edwards R, et al. Regulation of Toll-like receptor-2 expression in chronic hepatitis B by the precore protein. Hepatology. 2007;45:102-10 pubmed
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Balachandran P, Pugh N, Ma G, Pasco D. Toll-like receptor 2-dependent activation of monocytes by Spirulina polysaccharide and its immune enhancing action in mice. Int Immunopharmacol. 2006;6:1808-14 pubmed
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Shirey K, Jung J, Carlin J. Up-regulation of gamma interferon receptor expression due to Chlamydia-toll-like receptor interaction does not enhance signal transducer and activator of transcription 1 signaling. Infect Immun. 2006;74:6877-84 pubmed
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Hajishengallis G, Wang M, Harokopakis E, Triantafilou M, Triantafilou K. Porphyromonas gingivalis fimbriae proactively modulate beta2 integrin adhesive activity and promote binding to and internalization by macrophages. Infect Immun. 2006;74:5658-66 pubmed
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Yu L, Turner J, Buret A. LPS/CD14 activation triggers SGLT-1-mediated glucose uptake and cell rescue in intestinal epithelial cells via early apoptotic signals upstream of caspase-3. Exp Cell Res. 2006;312:3276-86 pubmed
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de Graaf R, Kloppenburg G, Kitslaar P, Bruggeman C, Stassen F. Human heat shock protein 60 stimulates vascular smooth muscle cell proliferation through Toll-like receptors 2 and 4. Microbes Infect. 2006;8:1859-65 pubmed
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Grabig A, Paclik D, Guzy C, Dankof A, Baumgart D, Erckenbrecht J, et al. Escherichia coli strain Nissle 1917 ameliorates experimental colitis via toll-like receptor 2- and toll-like receptor 4-dependent pathways. Infect Immun. 2006;74:4075-82 pubmed
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Kincade P. Supplying the demand for granulocytes. Nat Immunol. 2006;7:701-2 pubmed
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Harokopakis E, Albzreh M, Martin M, Hajishengallis G. TLR2 transmodulates monocyte adhesion and transmigration via Rac1- and PI3K-mediated inside-out signaling in response to Porphyromonas gingivalis fimbriae. J Immunol. 2006;176:7645-56 pubmed
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Rolland A, Jouvin Marche E, Viret C, Faure M, Perron H, Marche P. The envelope protein of a human endogenous retrovirus-W family activates innate immunity through CD14/TLR4 and promotes Th1-like responses. J Immunol. 2006;176:7636-44 pubmed
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Sugawara Y, Uehara A, Fujimoto Y, Kusumoto S, Fukase K, Shibata K, et al. Toll-like receptors, NOD1, and NOD2 in oral epithelial cells. J Dent Res. 2006;85:524-9 pubmed
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Remer K, Brcic M, Sauter K, Jungi T. Human monocytoid cells as a model to study Toll-like receptor-mediated activation. J Immunol Methods. 2006;313:1-10 pubmed
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Jung S, Yang C, Lee J, Shin A, Jung S, Son J, et al. The mycobacterial 38-kilodalton glycolipoprotein antigen activates the mitogen-activated protein kinase pathway and release of proinflammatory cytokines through Toll-like receptors 2 and 4 in human monocytes. Infect Immun. 2006;74:2686-96 pubmed
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Karimi K, Sarir H, Mortaz E, Smit J, Hosseini H, De Kimpe S, et al. Toll-like receptor-4 mediates cigarette smoke-induced cytokine production by human macrophages. Respir Res. 2006;7:66 pubmed
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LeBouder E, Rey Nores J, Raby A, Affolter M, Vidal K, Thornton C, et al. Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk. J Immunol. 2006;176:3742-52 pubmed
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Sadeghi K, Wessner B, Laggner U, Ploder M, Tamandl D, Friedl J, et al. Vitamin D3 down-regulates monocyte TLR expression and triggers hyporesponsiveness to pathogen-associated molecular patterns. Eur J Immunol. 2006;36:361-70 pubmed
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Zhang Z, Liu R, Noordhoek J, Kauffman H. Interaction of airway epithelial cells (A549) with spores and mycelium of Aspergillus fumigatus. J Infect. 2005;51:375-82 pubmed
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Parker L, Jones E, Prince L, Dower S, Whyte M, Sabroe I. Endotoxin tolerance induces selective alterations in neutrophil function. J Leukoc Biol. 2005;78:1301-5 pubmed
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Hadley J, Wang J, Foster S, Thiemermann C, Hinds C. Peptidoglycan of Staphylococcus aureus upregulates monocyte expression of CD14, Toll-like receptor 2 (TLR2), and TLR4 in human blood: possible implications for priming of lipopolysaccharide signaling. Infect Immun. 2005;73:7613-9 pubmed
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MacRedmond R, Greene C, Taggart C, McElvaney N, O Neill S. Respiratory epithelial cells require Toll-like receptor 4 for induction of human beta-defensin 2 by lipopolysaccharide. Respir Res. 2005;6:116 pubmed
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Viemann D, Dubbel G, Schleifenbaum S, Harms E, Sorg C, Roth J. Expression of toll-like receptors in neonatal sepsis. Pediatr Res. 2005;58:654-9 pubmed
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product information
Product Type :
Antibody
Product Name :
CD284 (TLR4) Monoclonal Antibody (HTA125), PE-Cyanine7, eBioscience
Catalog # :
25-9917-42
Quantity :
100 Tests
Price :
US 441.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 5 uL (1 ug)/test
Species :
Human
Clone :
HTA125
Isotype :
IgG2a, kappa
Storage :
4 C, store in dark, DO NOT FREEZE!
Description :
TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 5 uL (1 ug)/test
Aliases :
ARMD10; CD284; EGK_07381; homolog of Drosophila toll; hToll; lipopolysaccharide response; Lps; Ly87; Ran/M1; Rasl2-8; Tlr4; TLR-4; TOLL; toll like receptor 4; toll/interleukin-l receptor (TIR) domain; toll4; toll-like receptor 4; toll-like receptor 4 immunity-related protein; Toll-like receptor 4-like protein; Toll-like receptor4 protein
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com81 Wyman Street
Waltham, MA USA 02451
800-678-5599
headquarters: USA
questions and comments