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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD326 (EpCAM) Monoclonal Antibody (G8.8), PE-Cyanine7, eBioscience
catalog :
25-5791-80
quantity :
25 µg
price :
US 151
clonality :
monoclonal
host :
rat
conjugate :
PE/Cy7
clone name :
G8.8
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 60
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:3000; loading ...; fig 1s1a, 5a
Lopes N, Boucherit N, Santamaria J, Provin N, Charaix J, Ferrier P, et al. Thymocytes trigger self-antigen-controlling pathways in immature medullary thymic epithelial stages. elife. 2022;11: pubmed publisher
  • flow cytometry; mouse; loading ...
Liu M, Rao H, Liu J, Li X, Feng W, Gui L, et al. The histone methyltransferase SETD2 modulates oxidative stress to attenuate experimental colitis. Redox Biol. 2021;43:102004 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Uccellini M, Garcia Sastre A. ISRE-Reporter Mouse Reveals High Basal and Induced Type I IFN Responses in Inflammatory Monocytes. Cell Rep. 2018;25:2784-2796.e3 pubmed publisher
  • flow cytometry; mouse; loading ...; fig S1A
Marichal T, Gaudenzio N, El Abbas S, Sibilano R, Zurek O, Starkl P, et al. Guanine nucleotide exchange factor RABGEF1 regulates keratinocyte-intrinsic signaling to maintain skin homeostasis. J Clin Invest. 2016;126:4497-4515 pubmed publisher
  • flow cytometry; mouse; fig 1
Wang N, Dong B, Quan Y, Chen Q, Chu M, Xu J, et al. Regulation of Prostate Development and Benign Prostatic Hyperplasia by Autocrine Cholinergic Signaling via Maintaining the Epithelial Progenitor Cells in Proliferating Status. Stem Cell Reports. 2016;6:668-678 pubmed publisher
  • flow cytometry; mouse; fig 3
Borkent M, Bennett B, Lackford B, Bar Nur O, Brumbaugh J, Wang L, et al. A Serial shRNA Screen for Roadblocks to Reprogramming Identifies the Protein Modifier SUMO2. Stem Cell Reports. 2016;6:704-716 pubmed publisher
  • flow cytometry; mouse; 1:50
Causton B, Ramadas R, CHO J, Jones K, Pardo Saganta A, Rajagopal J, et al. CARMA3 Is Critical for the Initiation of Allergic Airway Inflammation. J Immunol. 2015;195:683-94 pubmed publisher
Mauduit O, Aure M, Delcroix V, Basova L, Srivastava A, Umazume T, et al. A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands. Cell Rep. 2022;39:110663 pubmed publisher
Cordero Espinoza L, Dowbaj A, Kohler T, Strauss B, Sarlidou O, Belenguer G, et al. Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation. Cell Stem Cell. 2021;28:1907-1921.e8 pubmed publisher
Goga A, Yagabasan B, Herrmanns K, Godbersen S, Silva P, Denzler R, et al. miR-802 regulates Paneth cell function and enterocyte differentiation in the mouse small intestine. Nat Commun. 2021;12:3339 pubmed publisher
Freter R, Falletta P, Omrani O, Rasa M, Herbert K, Annunziata F, et al. Establishment of a fluorescent reporter of RNA-polymerase II activity to identify dormant cells. Nat Commun. 2021;12:3318 pubmed publisher
Giraud J, Foroutan M, Boubaker Vitre J, Grillet F, Homayed Z, Jadhav U, et al. Progastrin production transitions from Bmi1+/Prox1+ to Lgr5high cells during early intestinal tumorigenesis. Transl Oncol. 2021;14:101001 pubmed publisher
Tang D, Xu X, Ying J, Xie T, Cao G. Transfer of metastatic traits via miR-200c in extracellular vesicles derived from colorectal cancer stem cells is inhibited by atractylenolide I. Clin Transl Med. 2020;10:e139 pubmed publisher
Ko H, Hong S, Verma R, Jung J, Lee M, Kim N, et al. Dietary Glucose Consumption Promotes RALDH Activity in Small Intestinal CD103+CD11b+ Dendritic Cells. Front Immunol. 2020;11:1897 pubmed publisher
Liu X, Boyer M, Holmgren A, Shin S. Legionella-Infected Macrophages Engage the Alveolar Epithelium to Metabolically Reprogram Myeloid Cells and Promote Antibacterial Inflammation. Cell Host Microbe. 2020;28:683-698.e6 pubmed publisher
Wang X, Yang L, Wang Y, Xu Z, Feng Y, Zhang J, et al. Comparative analysis of cell lineage differentiation during hepatogenesis in humans and mice at the single-cell transcriptome level. Cell Res. 2020;30:1109-1126 pubmed publisher
Kazakevych J, Denizot J, Liebert A, Portovedo M, Mosavie M, Jain P, et al. Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium. Genome Biol. 2020;21:64 pubmed publisher
Xie Y, Ding F, Di W, Lv Y, Xia F, Sheng Y, et al. Impact of a high‑fat diet on intestinal stem cells and epithelial barrier function in middle‑aged female mice. Mol Med Rep. 2020;21:1133-1144 pubmed publisher
Lee J, Hall J, Kroehling L, Wu L, Najar T, Nguyen H, et al. Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease. Cell. 2020;180:79-91.e16 pubmed publisher
Erlangga Z, Wolff K, Poth T, Peltzer A, Nahnsen S, Spielberg S, et al. Potent Antitumor Activity of Liposomal Irinotecan in an Organoid- and CRISPR-Cas9-Based Murine Model of Gallbladder Cancer. Cancers (Basel). 2019;11: pubmed publisher
Wang J, Wang D, Chu K, Li W, Zeng Y. Procr-expressing progenitor cells are responsible for murine ovulatory rupture repair of ovarian surface epithelium. Nat Commun. 2019;10:4966 pubmed publisher
Ouadah Y, Rojas E, Riordan D, Capostagno S, Kuo C, Krasnow M. Rare Pulmonary Neuroendocrine Cells Are Stem Cells Regulated by Rb, p53, and Notch. Cell. 2019;179:403-416.e23 pubmed publisher
Antunes K, Fachi J, de Paula R, da Silva E, Pral L, dos Santos A, et al. Microbiota-derived acetate protects against respiratory syncytial virus infection through a GPR43-type 1 interferon response. Nat Commun. 2019;10:3273 pubmed publisher
Pfefferle A, Darr D, Calhoun B, Mott K, Rosen J, Perou C. The MMTV-Wnt1 murine model produces two phenotypically distinct subtypes of mammary tumors with unique therapeutic responses to an EGFR inhibitor. Dis Model Mech. 2019;12: pubmed publisher
Srivastava S, Salter A, Liggitt D, Yechan Gunja S, Sarvothama M, Cooper K, et al. Logic-Gated ROR1 Chimeric Antigen Receptor Expression Rescues T Cell-Mediated Toxicity to Normal Tissues and Enables Selective Tumor Targeting. Cancer Cell. 2019;35:489-503.e8 pubmed publisher
Saborowski A, Wolff K, Spielberg S, Beer B, Hartleben B, Erlangga Z, et al. Murine Liver Organoids as a Genetically Flexible System to Study Liver Cancer InVivo and In Vitro. Hepatol Commun. 2019;3:423-436 pubmed publisher
Jones J, Brindley C, Elder N, Myers M, Rajala M, Dekaney C, et al. Cellular Plasticity of Defa4Cre-Expressing Paneth Cells in Response to Notch Activation and Intestinal Injury. Cell Mol Gastroenterol Hepatol. 2019;7:533-554 pubmed publisher
Glal D, Sudhakar J, Lu H, Liu M, Chiang H, Liu Y, et al. ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases. Front Immunol. 2018;9:2522 pubmed publisher
Moretti F, Klapproth S, Ruppert R, Margraf A, Weber J, Pick R, et al. Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus. elife. 2018;7: pubmed publisher
Li Z, Scott M, Brzoska T, Sundd P, Li Y, Billiar T, et al. Lung epithelial cell-derived IL-25 negatively regulates LPS-induced exosome release from macrophages. Mil Med Res. 2018;5:24 pubmed publisher
Zhao T, Fu Y, Zhu J, Liu Y, Zhang Q, Yi Z, et al. Single-Cell RNA-Seq Reveals Dynamic Early Embryonic-like Programs during Chemical Reprogramming. Cell Stem Cell. 2018;23:31-45.e7 pubmed publisher
Bockerstett K, Wong C, Koehm S, Ford E, DiPaolo R. Molecular Characterization of Gastric Epithelial Cells Using Flow Cytometry. Int J Mol Sci. 2018;19: pubmed publisher
Lopes N, Charaix J, Cedile O, Serge A, Irla M. Lymphotoxin α fine-tunes T cell clonal deletion by regulating thymic entry of antigen-presenting cells. Nat Commun. 2018;9:1262 pubmed publisher
Yui S, Azzolin L, Maimets M, Pedersen M, Fordham R, Hansen S, et al. YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. Cell Stem Cell. 2018;22:35-49.e7 pubmed publisher
Henderson M, Madenspacher J, Whitehead G, Thomas S, Aloor J, Gowdy K, et al. Effects of Orally Ingested Arsenic on Respiratory Epithelial Permeability to Bacteria and Small Molecules in Mice. Environ Health Perspect. 2017;125:097024 pubmed publisher
Yu L, Chen S, Luo N, He S. The C-terminus domain of the hepatitis B virus x protein stimulates the proliferation of mouse foetal hepatic progenitor cells, although it is not required for the formation of spheroids. Int J Mol Med. 2017;40:400-410 pubmed publisher
Wu B, An C, Li Y, Yin Z, Gong L, Li Z, et al. Reconstructing Lineage Hierarchies of Mouse Uterus Epithelial Development Using Single-Cell Analysis. Stem Cell Reports. 2017;9:381-396 pubmed publisher
Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, et al. DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. Nature. 2017;543:568-572 pubmed publisher
de Aguiar Vallim T, Lee E, Merriott D, Goulbourne C, Cheng J, Cheng A, et al. ABCG1 regulates pulmonary surfactant metabolism in mice and men. J Lipid Res. 2017;58:941-954 pubmed publisher
Mohapatra B, Zutshi N, An W, Goetz B, Arya P, Bielecki T, et al. An essential role of CBL and CBL-B ubiquitin ligases in mammary stem cell maintenance. Development. 2017;144:1072-1086 pubmed publisher
Liu Z, Guo W, Zhang D, Pang Y, Shi J, Wan S, et al. Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry. Sci Rep. 2016;6:39808 pubmed publisher
Shi Y, Wu W, Chai Q, Li Q, Hou Y, Xia H, et al. LTβR controls thymic portal endothelial cells for haematopoietic progenitor cell homing and T-cell regeneration. Nat Commun. 2016;7:12369 pubmed publisher
Maimets M, Rocchi C, Bron R, Pringle S, Kuipers J, Giepmans B, et al. Long-Term In Vitro Expansion of Salivary Gland Stem Cells Driven by Wnt Signals. Stem Cell Reports. 2016;6:150-62 pubmed publisher
Pardo Saganta A, Tata P, Law B, Saez B, Chow R, Prabhu M, et al. Parent stem cells can serve as niches for their daughter cells. Nature. 2015;523:597-601 pubmed publisher
Williams B, Tebbutt N, Buchert M, Putoczki T, Doggett K, Bao S, et al. Glycoprotein A33 deficiency: a new mouse model of impaired intestinal epithelial barrier function and inflammatory disease. Dis Model Mech. 2015;8:805-15 pubmed publisher
Farin A, Manzo N, Kirsch D, Stripp B. Low- and high-LET radiation drives clonal expansion of lung progenitor cells in vivo. Radiat Res. 2015;183:124-32 pubmed publisher
Clatworthy M, Aronin C, Mathews R, Morgan N, Smith K, Germain R. Immune complexes stimulate CCR7-dependent dendritic cell migration to lymph nodes. Nat Med. 2014;20:1458-63 pubmed publisher
Walmsley G, Rinkevich Y, Hu M, Montoro D, Lo D, McArdle A, et al. Live fibroblast harvest reveals surface marker shift in vitro. Tissue Eng Part C Methods. 2015;21:314-21 pubmed publisher
Danzl N, Jeong S, Choi Y, Alexandropoulos K. Identification of novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6. PLoS ONE. 2014;9:e86129 pubmed publisher
Tata P, Mou H, Pardo Saganta A, Zhao R, Prabhu M, Law B, et al. Dedifferentiation of committed epithelial cells into stem cells in vivo. Nature. 2013;503:218-23 pubmed publisher
Magness S, Puthoff B, Crissey M, Dunn J, Henning S, Houchen C, et al. A multicenter study to standardize reporting and analyses of fluorescence-activated cell-sorted murine intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2013;305:G542-51 pubmed publisher
Barkauskas C, Cronce M, Rackley C, Bowie E, Keene D, Stripp B, et al. Type 2 alveolar cells are stem cells in adult lung. J Clin Invest. 2013;123:3025-36 pubmed publisher
Goldman O, Han S, Sourisseau M, Sourrisseau M, Dziedzic N, Hamou W, et al. KDR identifies a conserved human and murine hepatic progenitor and instructs early liver development. Cell Stem Cell. 2013;12:748-60 pubmed publisher
El Zaatari M, Kao J, Tessier A, Bai L, Hayes M, Fontaine C, et al. Gli1 deletion prevents Helicobacter-induced gastric metaplasia and expansion of myeloid cell subsets. PLoS ONE. 2013;8:e58935 pubmed publisher
Hwang J, Kita R, Kwon H, Choi E, Lee S, Udey M, et al. Epidermal ablation of Dlx3 is linked to IL-17-associated skin inflammation. Proc Natl Acad Sci U S A. 2011;108:11566-71 pubmed publisher
Cook B, Liu S, Evans T. Smad1 signaling restricts hematopoietic potential after promoting hemangioblast commitment. Blood. 2011;117:6489-97 pubmed publisher
Dooley J, Erickson M, Farr A. An organized medullary epithelial structure in the normal thymus expresses molecules of respiratory epithelium and resembles the epithelial thymic rudiment of nude mice. J Immunol. 2005;175:4331-7 pubmed
Nelson A, Dunn R, Peach R, Aruffo A, Farr A. The murine homolog of human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells. Eur J Immunol. 1996;26:401-8 pubmed
Borkowski T, Nelson A, Farr A, Udey M. Expression of gp40, the murine homologue of human epithelial cell adhesion molecule (Ep-CAM), by murine dendritic cells. Eur J Immunol. 1996;26:110-4 pubmed
Farr A, Nelson A, Truex J, Hosier S. Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium. J Histochem Cytochem. 1991;39:645-53 pubmed
product information
Product Type :
Antibody
Product Name :
CD326 (EpCAM) Monoclonal Antibody (G8.8), PE-Cyanine7, eBioscience
Catalog # :
25-5791-80
Quantity :
25 µg
Price :
US 151
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.125 µg/test
Species :
Mouse
Clone :
G8.8
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.125 µg/test
Aliases :
adenocarcinoma-associated antigen; CD326; cell surface glycoprotein Trop-1; DIAR5; EGP; EGP-2; EGP314; EGP40; EPCAM; Ep-CAM; EpCAM1; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; ESA; GA733-2; gp40; hEGP314; HNPCC8; human epithelial glycoprotein-2; KS 1/4 antigen; KS1/4; KSA; Ly74; lymphocyte antigen 74; M1S2; M4S1; major gastrointestinal tumor-associated protein GA733-2; mEGP314; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; panepithelial glycoprotein 314; protein 289A; Protein D5.7A; Tacsd1; Tacstd1; TROP1; Trop-1 protein; Tumor-associated calcium signal transducer 1
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA