product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
IgM Monoclonal Antibody (II/41), PE-Cyanine7, eBioscience™
catalog :
25-5790-81
quantity :
50 µg
price :
US 309.00
clonality :
monoclonal
host :
rat
conjugate :
PE/Cy7
clone name :
II/41
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 47
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; loading ...; fig 3
Sanges S, Jendoubi M, Kavian N, Hauspie C, Speca S, Crave J, et al. B Cell Homeostasis and Functional Properties Are Altered in an Hypochlorous Acid-Induced Murine Model of Systemic Sclerosis. Front Immunol. 2017;8:53 pubmed publisher
Fallet B, Hao Y, Florova M, Cornille K, de Los Aires A, Girelli Zubani G, et al. Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses. Cell Rep. 2020;30:1013-1026.e7 pubmed publisher
Schoeler K, Aufschnaiter A, Messner S, Derudder E, Herzog S, Villunger A, et al. TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells. FEBS J. 2019;286:3566-3581 pubmed publisher
Weng Q, Hu F, Zhang M, Dong Y, Lv C, Wang Y, et al. A protocol for generating induced T cells by reprogramming B cells in vivo. Cell Regen (Lond). 2018;7:7-15 pubmed publisher
Schager A, Dominguez Medina C, Necchi F, Micoli F, Goh Y, Goodall M, et al. IgG Responses to Porins and Lipopolysaccharide within an Outer Membrane-Based Vaccine against Nontyphoidal Salmonella Develop at Discordant Rates. MBio. 2018;9: pubmed publisher
Ayre D, Chute I, Joy A, Barnett D, Hogan A, Grüll M, et al. CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo. Sci Rep. 2017;7:8642 pubmed publisher
Dang J, Bian X, Ma X, Li J, Long F, Shan S, et al. ORMDL3 Facilitates the Survival of Splenic B Cells via an ATF6?-Endoplasmic Reticulum Stress-Beclin1 Autophagy Regulatory Pathway. J Immunol. 2017;199:1647-1659 pubmed publisher
Dave K, Sur I, Yan J, Zhang J, Kaasinen E, Zhong F, et al. Mice deficient of Myc super-enhancer region reveal differential control mechanism between normal and pathological growth. elife. 2017;6: pubmed publisher
Schweighoffer E, Nys J, Vanes L, Smithers N, Tybulewicz V. TLR4 signals in B lymphocytes are transduced via the B cell antigen receptor and SYK. J Exp Med. 2017;214:1269-1280 pubmed publisher
Lauder S, Tyrrell V, Allen Redpath K, Aldrovandi M, Gray D, Collins P, et al. Myeloid 12/15-LOX regulates B cell numbers and innate immune antibody levels in vivo. Wellcome Open Res. 2017;2:1 pubmed publisher
Robinson M, Prout M, Mearns H, Kyle R, Camberis M, Forbes Blom E, et al. IL-4 Haploinsufficiency Specifically Impairs IgE Responses against Allergens in Mice. J Immunol. 2017;198:1815-1822 pubmed publisher
Ikawa T, Masuda K, Endo T, Endo M, Isono K, Koseki Y, et al. Conversion of T cells to B cells by inactivation of polycomb-mediated epigenetic suppression of the B-lineage program. Genes Dev. 2016;30:2475-2485 pubmed
Forster M, Farrington K, Petrov J, Belle J, Mindt B, Witalis M, et al. MYSM1-dependent checkpoints in B cell lineage differentiation and B cell-mediated immune response. J Leukoc Biol. 2017;101:643-654 pubmed publisher
Sabouri Z, Perotti S, Spierings E, Humburg P, Yabas M, Bergmann H, et al. IgD attenuates the IgM-induced anergy response in transitional and mature B cells. Nat Commun. 2016;7:13381 pubmed publisher
Jones D, Gaudette B, Wilmore J, Chernova I, Bortnick A, Weiss B, et al. mTOR has distinct functions in generating versus sustaining humoral immunity. J Clin Invest. 2016;126:4250-4261 pubmed publisher
O Leary C, Riling C, Spruce L, Ding H, Kumar S, Deng G, et al. Ndfip-mediated degradation of Jak1 tunes cytokine signalling to limit expansion of CD4+ effector T cells. Nat Commun. 2016;7:11226 pubmed publisher
Sei J, Waters R, Kenney M, Barlow J, Golde W. Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle. PLoS ONE. 2016;11:e0152192 pubmed publisher
Cheong T, Compagno M, Chiarle R. Editing of mouse and human immunoglobulin genes by CRISPR-Cas9 system. Nat Commun. 2016;7:10934 pubmed publisher
Lykken J, Horikawa M, Minard Colin V, Kamata M, Miyagaki T, Poe J, et al. Galectin-1 drives lymphoma CD20 immunotherapy resistance: validation of a preclinical system to identify resistance mechanisms. Blood. 2016;127:1886-95 pubmed publisher
Yabas M, Jing W, Shafik S, Bröer S, Enders A. ATP11C Facilitates Phospholipid Translocation across the Plasma Membrane of All Leukocytes. PLoS ONE. 2016;11:e0146774 pubmed publisher
Lu G, Duan J, Shu S, Wang X, Gao L, Guo J, et al. Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining. Proc Natl Acad Sci U S A. 2016;113:1256-60 pubmed publisher
Nadrah K, Beck T, Pereira J. Immature B Cell Egress from Bone Marrow Is SOCS3 Independent. PLoS ONE. 2015;10:e0136061 pubmed publisher
Lowe K, Navarro Núñez L, Bénézech C, Nayar S, Kingston B, Nieswandt B, et al. The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state. Eur J Immunol. 2015;45:2484-93 pubmed publisher
Weindel C, Richey L, Bolland S, Mehta A, Kearney J, Huber B. B cell autophagy mediates TLR7-dependent autoimmunity and inflammation. Autophagy. 2015;11:1010-24 pubmed publisher
Liu X, Qian Y, Jung Y, Chen P. Isolation and immunomodulatory activity of bursal peptide, a novel bursal peptide from the chicken bursa of Fabricius. J Vet Sci. 2015;16:501-7 pubmed publisher
Jacque E, Schweighoffer E, Tybulewicz V, Ley S. BAFF activation of the ERK5 MAP kinase pathway regulates B cell survival. J Exp Med. 2015;212:883-92 pubmed publisher
Cho J, Seo J, Lim C, Yang L, Shiratsuchi T, Lee M, et al. Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis. Cell Death Differ. 2015;22:1437-50 pubmed publisher
Reynolds A, Kuraoka M, Kelsoe G. Natural IgM is produced by CD5- plasma cells that occupy a distinct survival niche in bone marrow. J Immunol. 2015;194:231-42 pubmed publisher
Jurkin J, Henkel T, Nielsen A, Minnich M, Popow J, Kaufmann T, et al. The mammalian tRNA ligase complex mediates splicing of XBP1 mRNA and controls antibody secretion in plasma cells. EMBO J. 2014;33:2922-36 pubmed publisher
Jacque E, Schweighoffer E, Visekruna A, Papoutsopoulou S, Janzen J, Zillwood R, et al. IKK-induced NF-κB1 p105 proteolysis is critical for B cell antibody responses to T cell-dependent antigen. J Exp Med. 2014;211:2085-101 pubmed publisher
Bestas B, Moreno P, Blomberg K, Mohammad D, Saleh A, Sutlu T, et al. Splice-correcting oligonucleotides restore BTK function in X-linked agammaglobulinemia model. J Clin Invest. 2014;124:4067-81 pubmed publisher
Mich J, Signer R, Nakada D, Pineda A, Burgess R, Vue T, et al. Prospective identification of functionally distinct stem cells and neurosphere-initiating cells in adult mouse forebrain. elife. 2014;3:e02669 pubmed publisher
Rouaud P, Saintamand A, Saad F, Carrion C, Lecardeur S, Cogne M, et al. Elucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region. J Exp Med. 2014;211:975-85 pubmed publisher
Bénézech C, Nayar S, Finney B, Withers D, Lowe K, Desanti G, et al. CLEC-2 is required for development and maintenance of lymph nodes. Blood. 2014;123:3200-7 pubmed publisher
Nakamura T, Hamuro J, Takaishi M, Simmons S, Maruyama K, Zaffalon A, et al. LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis. J Clin Invest. 2014;124:385-97 pubmed publisher
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Lindner J, Wong C, Möller A, Nielsen P. A C-terminal acidic domain regulates degradation of the transcriptional coactivator Bob1. Mol Cell Biol. 2013;33:4628-40 pubmed publisher
Carofino B, Ayanga B, Justice M. A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL). Dis Model Mech. 2013;6:1494-506 pubmed publisher
Yu M, Chen Y, He Y, Podd A, Fu G, Wright J, et al. Critical role of B cell lymphoma 10 in BAFF-regulated NF-?B activation and survival of anergic B cells. J Immunol. 2012;189:5185-93 pubmed publisher
Shen S, Manser T. Direct reduction of antigen receptor expression in polyclonal B cell populations developing in vivo results in light chain receptor editing. J Immunol. 2012;188:47-56 pubmed publisher
Robbins G, Knight K. Mechanism for pre-B cell loss in VH-mutant rabbits. J Immunol. 2011;187:4714-20 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
IgM Monoclonal Antibody (II/41), PE-Cyanine7, eBioscience™
Catalog # :
25-5790-81
Quantity :
50 µg
Price :
US 309.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.125 µg/test
Species :
Mouse
Clone :
II/41
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.125 µg/test
Aliases :
AGM1; AI326478; Igh6; Igh-6; Ighm; Igh-M; Igm; immunoglobulin heavy constant mu; MU; muH; VH
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA