product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD45.1 Monoclonal Antibody (A20), PE-Cyanine7, eBioscience
catalog :
25-0453-82
quantity :
100 ug
price :
US 259.00
clonality :
monoclonal
host :
mouse
conjugate :
PE/Cy7
clone name :
A20
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 120
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s1a, s1e
Garnier L, Pick R, Montorfani J, Sun M, Brighouse D, Liaudet N, et al. IFN-γ-dependent tumor-antigen cross-presentation by lymphatic endothelial cells promotes their killing by T cells and inhibits metastasis. Sci Adv. 2022;8:eabl5162 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7c, 7d, 7e
Lu L, Li T, Feng X, Liu Z, Liu Y, Chao T, et al. Excessive immunosuppression by regulatory T cells antagonizes T cell response to schistosome infection in PD-1-deficient mice. PLoS Pathog. 2022;18:e1010596 pubmed publisher
  • flow cytometry; mouse; fig s1
Poletto E, Colella P, Pimentel Vera L, Khan S, Tomatsu S, Baldo G, et al. Improved engraftment and therapeutic efficacy by human genome-edited hematopoietic stem cells with Busulfan-based myeloablation. Mol Ther Methods Clin Dev. 2022;25:392-409 pubmed publisher
  • flow cytometry; mouse
Webb L, Fra Bido S, Innocentin S, Matheson L, Attaf N, Bignon A, et al. Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ. Aging Cell. 2021;20:e13295 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2g, 4l, e7l
Esterházy D, Canesso M, Mesin L, Muller P, de Castro T, Lockhart A, et al. Compartmentalized gut lymph node drainage dictates adaptive immune responses. Nature. 2019;569:126-130 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2i
Chakarov S, Lim H, Tan L, Lim S, See P, Lum J, et al. Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches. Science. 2019;363: pubmed publisher
  • flow cytometry; mouse; 1:50; loading ...; fig s4c
Ge Y, Schuster M, Pundhir S, Rapin N, Bagger F, Sidiropoulos N, et al. The splicing factor RBM25 controls MYC activity in acute myeloid leukemia. Nat Commun. 2019;10:172 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Thomas D, Clare S, Sowerby J, Pardo M, Juss J, Goulding D, et al. Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity. J Exp Med. 2017;214:1111-1128 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3g
Pishesha N, Bilate A, Wibowo M, Huang N, Li Z, Deshycka R, et al. Engineered erythrocytes covalently linked to antigenic peptides can protect against autoimmune disease. Proc Natl Acad Sci U S A. 2017;114:3157-3162 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s8b
Ho T, Warr M, Adelman E, Lansinger O, Flach J, Verovskaya E, et al. Autophagy maintains the metabolism and function of young and old stem cells. Nature. 2017;543:205-210 pubmed publisher
  • flow cytometry; mouse
Luchsinger L, de Almeida M, Corrigan D, Mumau M, Snoeck H. Mitofusin 2 maintains haematopoietic stem cells with extensive lymphoid potential. Nature. 2016;529:528-31 pubmed publisher
  • flow cytometry; mouse; fig 1
Hu W, Dooley J, Chung S, Chandramohan D, Cimmino L, Mukherjee S, et al. miR-29a maintains mouse hematopoietic stem cell self-renewal by regulating Dnmt3a. Blood. 2015;125:2206-16 pubmed publisher
  • flow cytometry; mouse; fig s1
van Blijswijk J, Schraml B, Rogers N, Whitney P, Zelenay S, Acton S, et al. Altered lymph node composition in diphtheria toxin receptor-based mouse models to ablate dendritic cells. J Immunol. 2015;194:307-15 pubmed publisher
  • flow cytometry; human
Cartellieri M, Koristka S, Arndt C, Feldmann A, Stamova S, von Bonin M, et al. A novel ex vivo isolation and expansion procedure for chimeric antigen receptor engrafted human T cells. PLoS ONE. 2014;9:e93745 pubmed publisher
  • flow cytometry; mouse; fig 4
Haldar M, Kohyama M, So A, Kc W, Wu X, Briseño C, et al. Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages. Cell. 2014;156:1223-1234 pubmed publisher
  • flow cytometry; mouse
Fu G, Casas J, Rigaud S, Rybakin V, Lambolez F, Brzostek J, et al. Themis sets the signal threshold for positive and negative selection in T-cell development. Nature. 2013;504:441-5 pubmed publisher
  • flow cytometry; mouse; fig 3
Suliman S, Tan J, Xu K, Kousis P, Kowalski P, Chang G, et al. Notch3 is dispensable for thymocyte ?-selection and Notch1-induced T cell leukemogenesis. PLoS ONE. 2011;6:e24937 pubmed publisher
Li J, Chin C, Ying H, Meydan C, Teater M, Xia M, et al. Loss of CREBBP and KMT2D cooperate to accelerate lymphomagenesis and shape the lymphoma immune microenvironment. Nat Commun. 2024;15:2879 pubmed publisher
He T, Wang S, Li S, Shen H, Hou L, Liu Y, et al. Suppression of preadipocyte determination by SOX4 limits white adipocyte hyperplasia in obesity. iScience. 2023;26:106289 pubmed publisher
Scherer S, Oberle S, Kanev K, Gerullis A, Wu M, de Almeida G, et al. Pyrimidine de novo synthesis inhibition selectively blocks effector but not memory T cell development. Nat Immunol. 2023;24:501-515 pubmed publisher
Ma L, Tang Q, Gao X, Lee J, Lei R, Suzuki M, et al. Tet-mediated DNA demethylation regulates specification of hematopoietic stem and progenitor cells during mammalian embryogenesis. Sci Adv. 2022;8:eabm3470 pubmed publisher
Gunaseelan S, Ariffin M, Khanna S, Ooi M, Perera D, Chu J, et al. Pharmacological perturbation of CXCL1 signaling alleviates neuropathogenesis in a model of HEVA71 infection. Nat Commun. 2022;13:890 pubmed publisher
Zhang D, Gao X, Li H, Borger D, Wei Q, Yang E, et al. The microbiota regulates hematopoietic stem cell fate decisions by controlling iron availability in bone marrow. Cell Stem Cell. 2022;29:232-247.e7 pubmed publisher
Zotos D, Quast I, Li Wai Suen C, McKenzie C, Robinson M, Kan A, et al. The concerted change in the distribution of cell cycle phases and zone composition in germinal centers is regulated by IL-21. Nat Commun. 2021;12:7160 pubmed publisher
Young I, Wu B, Andricovich J, Chuang S, Li R, Tzatsos A, et al. Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling. Cell Rep. 2021;37:110036 pubmed publisher
Yang L, Chen W, Li L, Xiao Y, Fan S, Zhang Q, et al. Ddb1 Is Essential for the Expansion of CD4+ Helper T Cells by Regulating Cell Cycle Progression and Cell Death. Front Immunol. 2021;12:722273 pubmed publisher
Agarwal P, Li H, Choi K, Hueneman K, He J, Welner R, et al. TNF-α-induced alterations in stromal progenitors enhance leukemic stem cell growth via CXCR2 signaling. Cell Rep. 2021;36:109386 pubmed publisher
Li Z, Zhang W, Luo F, Li J, Yang W, Zhu B, et al. Allergen-Specific Treg Cells Upregulated by Lung-Stage S. japonicum Infection Alleviates Allergic Airway Inflammation. Front Cell Dev Biol. 2021;9:678377 pubmed publisher
Ho T, Dellorusso P, Verovskaya E, Bakker S, Flach J, Smith L, et al. Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions. J Exp Med. 2021;218: pubmed publisher
Seydoux E, Wan Y, Feng J, Wall A, Aljedani S, Homad L, et al. Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies. Cell Rep. 2021;35:109084 pubmed publisher
Cromer M, Camarena J, Martin R, Lesch B, Vakulskas C, Bode N, et al. Gene replacement of α-globin with β-globin restores hemoglobin balance in β-thalassemia-derived hematopoietic stem and progenitor cells. Nat Med. 2021;27:677-687 pubmed publisher
Lv K, Gong C, Antony C, Han X, Ren J, Donaghy R, et al. HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis. Cell Stem Cell. 2021;28:1275-1290.e9 pubmed publisher
Sharma R, Dever D, Lee C, Azizi A, Pan Y, Camarena J, et al. The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells. Nat Commun. 2021;12:472 pubmed publisher
Rivas M, Meydan C, Chin C, Challman M, Kim D, Bhinder B, et al. Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation. Nat Immunol. 2021;22:240-253 pubmed publisher
Smith M, Culver Cochran A, Adelman E, Rhyasen G, Ma A, Figueroa M, et al. TNFAIP3 Plays a Role in Aging of the Hematopoietic System. Front Immunol. 2020;11:536442 pubmed publisher
Fuster J, Zuriaga M, Zorita V, MacLauchlan S, Polackal M, Viana Huete V, et al. TET2-Loss-of-Function-Driven Clonal Hematopoiesis Exacerbates Experimental Insulin Resistance in Aging and Obesity. Cell Rep. 2020;33:108326 pubmed publisher
Kitamoto S, Nagao Kitamoto H, Jiao Y, Gillilland M, Hayashi A, Imai J, et al. The Intermucosal Connection between the Mouth and Gut in Commensal Pathobiont-Driven Colitis. Cell. 2020;182:447-462.e14 pubmed publisher
Scharenberg S, Poletto E, Lucot K, Colella P, Sheikali A, Montine T, et al. Engineering monocyte/macrophage-specific glucocerebrosidase expression in human hematopoietic stem cells using genome editing. Nat Commun. 2020;11:3327 pubmed publisher
Milner J, Toma C, He Z, Kurd N, Nguyen Q, McDonald B, et al. Heterogenous Populations of Tissue-Resident CD8+ T Cells Are Generated in Response to Infection and Malignancy. Immunity. 2020;52:808-824.e7 pubmed publisher
Rana A, de Almeida F, Paico Montero H, Gonzales Carazas M, Bortoluci K, Sad S, et al. RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes. Front Immunol. 2020;11:536 pubmed publisher
Zhang C, Jin H, Wang Y, Li C, Zhao X, Li Y, et al. Critical role of OX40 in drug-induced acute liver injury. Br J Pharmacol. 2020;177:3183-3196 pubmed publisher
Kang Y, Pietras E, Passegue E. Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia. J Exp Med. 2020;217: pubmed publisher
Chen C, Yu W, Tober J, Gao P, He B, Lee K, et al. Spatial Genome Re-organization between Fetal and Adult Hematopoietic Stem Cells. Cell Rep. 2019;29:4200-4211.e7 pubmed publisher
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Baptista A, Gola A, Huang Y, Milanez Almeida P, Torabi Parizi P, Urban J, et al. The Chemoattractant Receptor Ebi2 Drives Intranodal Naive CD4+ T Cell Peripheralization to Promote Effective Adaptive Immunity. Immunity. 2019;50:1188-1201.e6 pubmed publisher
Pyaram K, Kumar A, Kim Y, Noel S, Reddy S, Rabb H, et al. Keap1-Nrf2 System Plays an Important Role in Invariant Natural Killer T Cell Development and Homeostasis. Cell Rep. 2019;27:699-707.e4 pubmed publisher
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Zhu F, Willette Brown J, Song N, Lomada D, Song Y, Xue L, et al. Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis. Cell Host Microbe. 2017;21:478-493.e7 pubmed publisher
Downs Canner S, Berkey S, Delgoffe G, Edwards R, Curiel T, Odunsi K, et al. Suppressive IL-17A+Foxp3+ and ex-Th17 IL-17AnegFoxp3+ Treg cells are a source of tumour-associated Treg cells. Nat Commun. 2017;8:14649 pubmed publisher
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Coomes S, Kannan Y, Pelly V, Entwistle L, Guidi R, Perez Lloret J, et al. CD4+ Th2 cells are directly regulated by IL-10 during allergic airway inflammation. Mucosal Immunol. 2017;10:150-161 pubmed publisher
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Wu L, Li N, Zhang M, Xue S, Cassady K, Lin Q, et al. MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice. Proc Natl Acad Sci U S A. 2015;112:15994-9 pubmed publisher
Akhmetzyanova I, Zelinskyy G, Littwitz Salomon E, Malyshkina A, Dietze K, Streeck H, et al. CD137 Agonist Therapy Can Reprogram Regulatory T Cells into Cytotoxic CD4+ T Cells with Antitumor Activity. J Immunol. 2016;196:484-92 pubmed publisher
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Liu J, Wang L, Zhao F, Tseng S, Narayanan C, Shura L, et al. Pre-Clinical Development of a Humanized Anti-CD47 Antibody with Anti-Cancer Therapeutic Potential. PLoS ONE. 2015;10:e0137345 pubmed publisher
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Reis B, Rogoz A, Costa Pinto F, Taniuchi I, Mucida D. Mutual expression of the transcription factors Runx3 and ThPOK regulates intestinal CD4? T cell immunity. Nat Immunol. 2013;14:271-80 pubmed publisher
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Martins V, Ruggiero E, Schlenner S, Madan V, Schmidt M, Fink P, et al. Thymus-autonomous T cell development in the absence of progenitor import. J Exp Med. 2012;209:1409-17 pubmed publisher
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Gatto D, Wood K, Brink R. EBI2 operates independently of but in cooperation with CXCR5 and CCR7 to direct B cell migration and organization in follicles and the germinal center. J Immunol. 2011;187:4621-8 pubmed publisher
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Kaufman D, De Calisto J, Simmons N, Cruz A, Villablanca E, Mora J, et al. Vitamin A deficiency impairs vaccine-elicited gastrointestinal immunity. J Immunol. 2011;187:1877-83 pubmed publisher
Gardam S, Turner V, Anderton H, Limaye S, Basten A, Koentgen F, et al. Deletion of cIAP1 and cIAP2 in murine B lymphocytes constitutively activates cell survival pathways and inactivates the germinal center response. Blood. 2011;117:4041-51 pubmed publisher
Albring J, Sandau M, Rapaport A, Edelson B, Satpathy A, Mashayekhi M, et al. Targeting of B and T lymphocyte associated (BTLA) prevents graft-versus-host disease without global immunosuppression. J Exp Med. 2010;207:2551-9 pubmed publisher
Kaufman D, Bivas Benita M, Simmons N, Miller D, Barouch D. Route of adenovirus-based HIV-1 vaccine delivery impacts the phenotype and trafficking of vaccine-elicited CD8+ T lymphocytes. J Virol. 2010;84:5986-96 pubmed publisher
Astrakhan A, Ochs H, Rawlings D. Wiskott-Aldrich syndrome protein is required for homeostasis and function of invariant NKT cells. J Immunol. 2009;182:7370-80 pubmed publisher
Auffray C, Fogg D, Narni Mancinelli E, Senechal B, Trouillet C, Saederup N, et al. CX3CR1+ CD115+ CD135+ common macrophage/DC precursors and the role of CX3CR1 in their response to inflammation. J Exp Med. 2009;206:595-606 pubmed publisher
Pavlov V, Raedler H, Yuan S, Leisman S, Kwan W, Lalli P, et al. Donor deficiency of decay-accelerating factor accelerates murine T cell-mediated cardiac allograft rejection. J Immunol. 2008;181:4580-9 pubmed
Kaufman D, Liu J, Carville A, Mansfield K, Havenga M, Goudsmit J, et al. Trafficking of antigen-specific CD8+ T lymphocytes to mucosal surfaces following intramuscular vaccination. J Immunol. 2008;181:4188-98 pubmed
Rubinstein M, Lind N, Purton J, Filippou P, Best J, McGhee P, et al. IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune response. Blood. 2008;112:3704-12 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
CD45.1 Monoclonal Antibody (A20), PE-Cyanine7, eBioscience
Catalog # :
25-0453-82
Quantity :
100 ug
Price :
US 259.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5 ug/test
Species :
Mouse
Clone :
A20
Isotype :
IgG2a, kappa
Storage :
4 C, store in dark, DO NOT FREEZE!
Description :
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 ug/test
Aliases :
B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; Leukocyte common antigen; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA