CD27 Monoclonal Antibody (LG.7F9), PE-Cyanine7, eBioscience™ | Invitrogen 25-0271-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD27 Monoclonal Antibody (LG.7F9), PE-Cyanine7, eBioscience™

catalog :

25-0271-82

quantity :

100 µg

price :

US 309.00

clonality :

monoclonal

host :

hamsters

conjugate :

PE/Cy7

clone name :

LG.7F9

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:400; loading ...; fig s6b	Asrat S, Kaur N, Liu X, Ben L, Kajimura D, Murphy A, et al. Chronic allergen exposure drives accumulation of long-lived IgE plasma cells in the bone marrow, giving rise to serological memory. Sci Immunol. 2020;5: pubmed publisher
flow cytometry; human; loading ...; fig 3g	Moffett H, Harms C, Fitzpatrick K, Tooley M, Boonyaratanakornkit J, Taylor J. B cells engineered to express pathogen-specific antibodies protect against infection. Sci Immunol. 2019;4: pubmed publisher
flow cytometry; mouse; 1:200; loading ...; fig 1e	Wang F, Meng M, Mo B, Yang Y, Ji Y, Huang P, et al. Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function. Nat Commun. 2018;9:4874 pubmed publisher
flow cytometry; human; fig 1	Severson J, Serracino H, Mateescu V, Raeburn C, McIntyre R, Sams S, et al. PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer. Cancer Immunol Res. 2015;3:620-30 pubmed publisher
flow cytometry; mouse	Weston W, Zayas J, Perez R, George J, Jurecic R. Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets. Sci Rep. 2014;4:5199 pubmed publisher
	Wagner J, Wong P, Schappe T, Berrien Elliott M, Cubitt C, Jaeger N, et al. Stage-Specific Requirement for Eomes in Mature NK Cell Homeostasis and Cytotoxicity. Cell Rep. 2020;31:107720 pubmed publisher
	Ponzetta A, Carriero R, Carnevale S, Barbagallo M, Molgora M, Perucchini C, et al. Neutrophils Driving Unconventional T Cells Mediate Resistance against Murine Sarcomas and Selected Human Tumors. Cell. 2019;178:346-360.e24 pubmed publisher
	Cobbold S, Adams E, Howie D, Waldmann H. CD4+ T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium Development. Front Immunol. 2018;9:1381 pubmed publisher
	Kersten K, Coffelt S, Hoogstraat M, Verstegen N, Vrijland K, Ciampricotti M, et al. Mammary tumor-derived CCL2 enhances pro-metastatic systemic inflammation through upregulation of IL1β in tumor-associated macrophages. Oncoimmunology. 2017;6:e1334744 pubmed publisher
	Baranek T, Morello E, Valayer A, Aimar R, Bréa D, Henry C, et al. FHL2 Regulates Natural Killer Cell Development and Activation during Streptococcus pneumoniae Infection. Front Immunol. 2017;8:123 pubmed publisher
	Yang M, Chen S, Du J, He J, Wang Y, Li Z, et al. NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation. Nat Commun. 2016;7:12730 pubmed publisher
	Krneta T, Gillgrass A, Chew M, Ashkar A. The breast tumor microenvironment alters the phenotype and function of natural killer cells. Cell Mol Immunol. 2016;13:628-39 pubmed publisher
	Laidlaw B, Cui W, Amezquita R, Gray S, Guan T, Lu Y, et al. Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells. Nat Immunol. 2015;16:871-9 pubmed publisher
	Riese P, Trittel S, May T, Cicin Sain L, Chambers B, Guzman C. Activated NKT cells imprint NK-cell differentiation, functionality and education. Eur J Immunol. 2015;45:1794-807 pubmed publisher
	Jennings R, Grayson J, Barton E. Type I interferon signaling enhances CD8+ T cell effector function and differentiation during murine gammaherpesvirus 68 infection. J Virol. 2014;88:14040-9 pubmed publisher
	Salmond R, Brownlie R, Morrison V, Zamoyska R. The tyrosine phosphatase PTPN22 discriminates weak self peptides from strong agonist TCR signals. Nat Immunol. 2014;15:875-883 pubmed publisher
	Ribot J, Ribeiro S, Correia D, Sousa A, Silva Santos B. Human ?? thymocytes are functionally immature and differentiate into cytotoxic type 1 effector T cells upon IL-2/IL-15 signaling. J Immunol. 2014;192:2237-43 pubmed publisher
	Fogel L, Sun M, Geurs T, Carayannopoulos L, French A. Markers of nonselective and specific NK cell activation. J Immunol. 2013;190:6269-76 pubmed publisher
	Raki M, Beitnes A, Lundin K, Jahnsen J, Jahnsen F, Sollid L. Plasmacytoid dendritic cells are scarcely represented in the human gut mucosa and are not recruited to the celiac lesion. Mucosal Immunol. 2013;6:985-92 pubmed publisher
	French J, Kotnis G, Said S, Raeburn C, McIntyre R, Klopper J, et al. Programmed death-1+ T cells and regulatory T cells are enriched in tumor-involved lymph nodes and associated with aggressive features in papillary thyroid cancer. J Clin Endocrinol Metab. 2012;97:E934-43 pubmed publisher
	Tesselaar K, Gravestein L, van Schijndel G, Borst J, van Lier R. Characterization of murine CD70, the ligand of the TNF receptor family member CD27. J Immunol. 1997;159:4959-65 pubmed

product information

Product Type :

Antibody

Product Name :

CD27 Monoclonal Antibody (LG.7F9), PE-Cyanine7, eBioscience™

Catalog # :

25-0271-82

Quantity :

100 µg

Price :

US 309.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Armenian Hamster

Reactivity :

Human, Mouse, Rat

Applications :

Flow Cytometry: 0.5 µg/test

Species :

Human, Mouse, Rat

Clone :

LG.7F9

Isotype :

IgG

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 µg/test

Aliases :

CD antigen 27; CD27; CD27 antigen; CD27 molecule; CD27L receptor; S152; S152. LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T14; T-cell activation antigen CD27; TNFRSF7; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

more info or order :

Invitrogen product webpage