HSP27 Monoclonal Antibody (G3.1)
Invitrogen
catalog: MA3-015
mouse monoclonal (G3.1)
reactivity: human, mouse, rat, dogs
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 35

Western blot analysis of Heat Shock Protein 27 (HSP27) was performed by loading 50µg of the indicated whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP27 monoclonal antibody (Product # MA3-015) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
quantity: 100 uL
price: US 502.00
to the supplier
Phospho-HSP27 (Ser15) Polyclonal Antibody
Invitrogen
catalog: PA1-016
domestic rabbit polyclonal
reactivity: human, rat
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of Phospho Heat Shock Protein 27 (p-HSP27) pSer15 was performed by loading 50ug of HeLa cell lysates from untreated cells (left lane) or cells treated with 10uM Anisomycin (right lane) for 30 minutes and 15ul PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-016) at a dilution of 1:500 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

Immunoprecipitation of Phospho Heat Shock Protein (p-HSP27) pSer15 was performed on HeLa cells treated with 10uM Anisomysin for 30 minutes. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 3ug of p-HSP27 pSer15 polyclonal antibody (Product # PA1-016) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with Lane Marker Reducing Sample Buffer (Product # 39000). HeLa cell lysate (50ug) was loaded as a positive control (left lane). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-016) at a dilution of 1:500 overnight rotating at 4°C, washed in TBST, and probed with Clean-Blot IP Detection Reagent (Product # 21230) at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

Immunofluorescent analysis of Phospho-HSP27 (p-HSP27) pSer15 (green) in HeLa cells either left untreated (left panel) or treated with 10uM Anisomysin (right panel) for 30 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-016), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
quantity: 100 ug
price: US 419.00
to the supplier
HSP27 Polyclonal Antibody
Invitrogen
catalog: PA1-017
domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2
quantity: 100 ug
price: US 491.00
to the supplier
Phospho-HSP27 (Ser15) Polyclonal Antibody
Invitrogen
catalog: PA1-018
domestic rabbit polyclonal
reactivity: human, rat
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 1

Immunoprecipitation of Phospho Heat Shock Protein 27 (p-HSP27) pSer15 was performed on HeLa cells treated with 10uM Anisomysin for 30 minutes. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 3ug of p-HSP27 pSer15 polyclonal antibody (Product # PA1-018) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with Lane Marker Reducing Sample Buffer (Product # 39000). Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-018) at a dilution of 1:500 overnight rotating at 4°C, washed with TBST, and probed with Clean-Blot IP Detection Reagent (Product # 21230) at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

Immunofluorescent analysis of Phospho Heat Shock Protein 27 (p-HSP27) pSer15 (green) in HeLa cells either left untreated (left panel) or treated with 10uM Anisomysin (right panel) for 30 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-018) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Western blot analysis of Phospho Heat Shock Protein 27 (p-HSP27) pSer15 was performed by loading 50ug of HeLa cell lysates from untreated cells (left lane) or cells treated with 10uM Anisomycin (right lane) for 30 minutes and 15ul PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-018) at a dilution of 1:500 overnight at 4°C on a rocking platform, washed with TBS-0.1%Tween-20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
quantity: 100 ug
price: US 520.00
to the supplier
HSP25 Polyclonal Antibody
Invitrogen
catalog: PA1-25494
domestic rabbit polyclonal
reactivity: Chinese hamsters, guinea pig, mouse, rat, pigs
application: western blot, immunoprecipitation
quantity: 50 uL
price: US 514.00
to the supplier
HSP27 Polyclonal Antibody
Invitrogen
catalog: AHO1132
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 ug
price: US 500.00
to the supplier
Phospho-HSP27 (Ser78, Ser82) Polyclonal Antibody
Invitrogen
catalog: PA5-36912
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
HSP27 Polyclonal Antibody
Invitrogen
catalog: PA5-78010
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 514.00
to the supplier
Phospho-HSP27 (Ser82) Recombinant Rabbit Monoclonal Antibody (SN06-73)
Invitrogen
catalog: MA5-32266
domestic rabbit recombinant (SN06-73)
reactivity: human, mouse, rat
application: western blot
quantity: 100 uL
price: US 474.00
to the supplier
HSP27 Recombinant Rabbit Monoclonal Antibody (JJ09-13)
Invitrogen
catalog: MA5-32473
domestic rabbit recombinant (JJ09-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 530.00
to the supplier
Phospho-HSP27 (Ser82) Recombinant Rabbit Monoclonal Antibody (ARC1576)
Invitrogen
catalog: MA5-35873
domestic rabbit recombinant (ARC1576)
reactivity: human, rat
application: western blot, ELISA
quantity: 100 uL
price: US 491.00
to the supplier
HSP27 Recombinant Rabbit Monoclonal Antibody (ARC0531)
Invitrogen
catalog: MA5-35072
domestic rabbit recombinant (ARC0531)
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-HSP27 (Ser82) Monoclonal Antibody (GT357)
Invitrogen
catalog: MA5-35924
mouse monoclonal (GT357)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 444.00
to the supplier
HSP27 Monoclonal Antibody (10-8)
Invitrogen
catalog: MA5-44616
mouse monoclonal (8-Oct)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
HSP27 Monoclonal Antibody (8A7), APC
Invitrogen
catalog: MA5-45121
mouse monoclonal (8A7)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, dogs, bovine, domestic sheep
conjugate: APC
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 410.00
to the supplier
HSP27 Monoclonal Antibody (8A7), FITC
Invitrogen
catalog: MA5-45122
mouse monoclonal (8A7)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, dogs, bovine, domestic sheep
conjugate: FITC
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 410.00
to the supplier
HSP27 Monoclonal Antibody (8A7), PerCP
Invitrogen
catalog: MA5-45123
mouse monoclonal (8A7)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, dogs, bovine, domestic sheep
conjugate: PerCP
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 410.00
to the supplier
HSP27 Polyclonal Antibody
Invitrogen
catalog: PA5-143536
chicken polyclonal
reactivity: human, rat
application: western blot, immunocytochemistry
quantity: 500 uL
price: US 1614.00
to the supplier
HSP27 Polyclonal Antibody
Invitrogen
catalog: PA5-143570
chicken polyclonal
reactivity: human, rat
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 470.00
to the supplier