Invitrogen Tubal3 antibody

alpha Tubulin Monoclonal Antibody (236-10501)
Invitrogen
catalog: A11126

mouse monoclonal (236-10501)
reactivity: Leishmania , African green monkey, Cyrtanthus mackenii, kangaroo, malaria parasite P. falciparum, field poppy, hamsters, human, mouse, rat, bovine, fruit fly , Xenopus laevis
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 165

quantity: 50 ug
price: US 404.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A)
Invitrogen
catalog: 62204

mouse monoclonal (DM1A)
reactivity: freshwater planarian, African green monkey, guinea pig, Leishmania infantum, hamsters, human, mouse, rat, dogs, chicken, bovine, pigs , fruit fly , domestic sheep, Xenopus laevis
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, other
citations: 206

quantity: 40 uL
price: US 277.00
to the supplier

alpha Tubulin Monoclonal Antibody (YL1/2)
Invitrogen
catalog: MA1-80017

rat monoclonal (YL1/2)
reactivity: Dictyostelium discoideum, African green monkey, Saccharomyces cerevisiae S288C, human, mouse, rat, dogs, pigs , fruit fly , Xenopus laevis, domestic rabbit
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 85

quantity: 500 ug
price: US 524.00
to the supplier

Acetyl-alpha Tubulin (Lys40) Monoclonal Antibody (6-11B-1)
Invitrogen
catalog: 32-2700

mouse monoclonal (6-11B-1)
reactivity: Arthrotardigrada, , human, mouse, rat, dogs, chicken, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay
citations: 68

quantity: 100 ug
price: US 489.00
to the supplier

alpha Tubulin Monoclonal Antibody (B-5-1-2)
Invitrogen
catalog: 32-2500

mouse monoclonal (B-5-1-2)
reactivity: human, mouse, rat, dogs, brewer's yeast, thale cress, domestic rabbit
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
citations: 92

quantity: 100 ug
price: US 469.00
to the supplier

alpha Tubulin Monoclonal Antibody (TU-01)
Invitrogen
catalog: MA1-19162

mouse monoclonal (TU-01)
reactivity: Leishmania donovani, African green monkey, human, mouse, rat, bovine, pigs , Xenopus laevis
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 40

Immunofluorescent analysis of Phalloidin (blue) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.

Immunofluorescent analysis of Phalloidin (orange) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.

Western blot analysis of alpha-Tubulin was performed by loading various whole cell lysates onto a SDS-PAGE gel. Proteins were transferred to a membrane and probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a concentration of 1-2ug/ml (A) or a monoclonal antibody against a second target (B) for 1 hour at room temperature followed by an HRP-conjugated anti-mouse IgG secondary antibody. Detection was performed using a chemiluminescent substrate.
quantity: 100 ug
price: US 398.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), eBioscience
Invitrogen
catalog: 14-4502-80

mouse monoclonal (DM1A)
reactivity: human, mouse, rat, dogs, pigs
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 37

quantity: 25 ug
price: US 134.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), eBioscience
Invitrogen
catalog: 14-4502-82

quantity: 100 ug
price: US 356.00
to the supplier

alpha Tubulin Monoclonal Antibody (B-5-1-2), Alexa Fluor 488
Invitrogen
catalog: 322588

mouse monoclonal (B-5-1-2)
reactivity: human, mouse, rat
conjugate: AF488
application: immunohistochemistry, immunocytochemistry
citations: 39

quantity: 100 ug
price: US 562.00
to the supplier

alpha Tubulin Monoclonal Antibody (TU-01)
Invitrogen
catalog: 13-8000

mouse monoclonal (TU-01)
reactivity: African green monkey, human, mouse, rat, Xenopus laevis
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 61

quantity: 100 ug
price: US 451.00
to the supplier

alpha Tubulin Monoclonal Antibody (YOL1/34)
Invitrogen
catalog: MA1-80189

rat monoclonal (YOL1/34)
reactivity: Candida albicans, Saccharomyces cerevisiae S288C, human, mouse, fruit fly
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
citations: 23

quantity: 500 ug
price: US 520.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), Alexa Fluor 488, eBioscience
Invitrogen
catalog: 53-4502-80

mouse monoclonal (DM1A)
reactivity: human, mouse, rat, dogs, pigs
conjugate: AF488
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 21

quantity: 25 ug
price: US 177.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), Alexa Fluor 488, eBioscience
Invitrogen
catalog: 53-4502-82

mouse monoclonal (DM1A)
reactivity: human, mouse, rat, dogs, pigs
conjugate: AF488
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 22

quantity: 100 ug
price: US 486.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), eFluor 615, eBioscience
Invitrogen
catalog: 42-4502-82

mouse monoclonal (DM1A)
reactivity: human, mouse, rat, dogs, pigs
conjugate: eFluor 615
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 16

quantity: 100 ug
price: US 496.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), eBioscience
Invitrogen
catalog: 14-4502-95

quantity: 10 mg
price: US 3685.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), eBioscience
Invitrogen
catalog: 14-4502-37

quantity: 2 mg
price: US 1482.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), Biotin, eBioscience
Invitrogen
catalog: 13-4502-80

mouse monoclonal (DM1A)
reactivity: human, mouse, rat, dogs, pigs
conjugate: biotin
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 15

quantity: 25 ug
price: US 166.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), Biotin, eBioscience
Invitrogen
catalog: 13-4502-82

mouse monoclonal (DM1A)
reactivity: human, mouse, rat, dogs, pigs
conjugate: biotin
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 15

quantity: 100 ug
price: US 399.00
to the supplier

alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-19489

domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs
application: western blot, immunocytochemistry, flow cytometry
citations: 11

Immunoprecipitation of acetylated alpha-Tubulin was performed using whole cell lysates from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500ug of lysate with 3ug of an Acetyl Lysine monoclonal antibody (Product # MA1-2021) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with an alpha-Tubulin polyclonal antibody (Product # PA5-19489) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean-blot IP Detection Reagent (Product # 21230) at a dilution of 1:2000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).

Western blot analysis of HeLa Whole Cell Lysate using PA5-19489, alpha Tubulin primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19489 staining of Jurkat Whole Cell Lysate at a dilution of 1 ug/ml (Lane 2). PA5-19489 staining of A431 Whole Cell Lysate at a dilution of 1 ug/ml (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Immunofluorescent staining of HeLa cells using PA5-19489, anti-Alpha Tubulin antibody. The cells were fixed with methanol (100%) for 5 minutes and exposed to the primary antibody at a concentration of 1 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
quantity: 50 ug
price: US 478.00
to the supplier

alpha Tubulin Monoclonal Antibody (TU-02)
Invitrogen
catalog: MA1-19401

mouse monoclonal (TU-02)
reactivity: human, mouse, pigs
application: western blot, immunocytochemistry
citations: 4

quantity: 100 ug
price: US 437.00
to the supplier

alpha Tubulin Monoclonal Antibody (236-10501), Biotin
Invitrogen
catalog: A21371

mouse monoclonal (236-10501)
reactivity: human, mouse, bovine
conjugate: biotin
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 5

quantity: 50 ug
price: US 458.00
to the supplier

alpha Tubulin Monoclonal Antibody (TU-01), FITC
Invitrogen
catalog: MA1-19581

mouse monoclonal (TU-01)
reactivity: human, mouse, bovine, pigs
conjugate: FITC
application: western blot, immunocytochemistry, flow cytometry
citations: 4

quantity: 100 ug
price: US 491.00
to the supplier

alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA1-38814

domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 3

quantity: 1 mL
price: US 502.00
to the supplier

alpha Tubulin Monoclonal Antibody (GT114)
Invitrogen
catalog: MA5-31466

mouse monoclonal (GT114)
reactivity: human, mouse, rat, zebrafish , fruit fly
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 2

quantity: 100 uL
price: US 464.00
to the supplier

alpha Tubulin Monoclonal Antibody (TU-01), Alexa Fluor 555
Invitrogen
catalog: MA1-38000-A555

mouse monoclonal (TU-01)
reactivity: human, mouse
conjugate: Alexa Fluor 555
application: immunocytochemistry
citations: 1

quantity: 50 uL
price: US 425.00
to the supplier

alpha Tubulin Monoclonal Antibody (TU-01), Alexa Fluor 647
Invitrogen
catalog: MA1-38000-A647

mouse monoclonal (TU-01)
reactivity: human, mouse
conjugate: AF647
application: immunocytochemistry
citations: 1

quantity: 50 uL
price: US 425.00
to the supplier

alpha Tubulin Monoclonal Antibody (TU-16)
Invitrogen
catalog: MA1-19209

mouse monoclonal (TU-16)
reactivity: human, mouse, rat, pigs
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 100 ug
price: US 463.00
to the supplier

alpha Tubulin Monoclonal Antibody (TU-01), Alexa Fluor 488
Invitrogen
catalog: MA1-38000-A488

mouse monoclonal (TU-01)
reactivity: human, mouse
conjugate: AF488
application: immunocytochemistry

quantity: 50 uL
price: US 496.00
to the supplier

TUBAL3 Monoclonal Antibody (OTI4C5)
Invitrogen
catalog: MA5-25772

mouse monoclonal (OTI4C5)
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 514.00
to the supplier

alpha Tubulin Recombinant Rabbit Monoclonal Antibody (RM113), Biotin
Invitrogen
catalog: MA5-27888

domestic rabbit recombinant (RM113)
reactivity: human, mouse, rat
conjugate: biotin
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 50 uL
price: US 484.00
to the supplier

alpha Tubulin Recombinant Rabbit Monoclonal Antibody (RM113)
Invitrogen
catalog: MA5-27889

domestic rabbit recombinant (RM113)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation

quantity: 100 uL
price: US 480.00
to the supplier

alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-85157

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-85922

domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 469.00
to the supplier

Acetyl-alpha Tubulin (Lys40) Polyclonal Antibody
Invitrogen
catalog: PA5-105102

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 440.00
to the supplier

alpha Tubulin Recombinant Rabbit Monoclonal Antibody (3P2D9)
Invitrogen
catalog: MA5-38163

domestic rabbit recombinant (3P2D9)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 484.00
to the supplier

alpha Tubulin Monoclonal Antibody (11B4E1)
Invitrogen
catalog: MA5-38221

mouse monoclonal (11B4E1)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

alpha Tubulin Monoclonal Antibody (YOL1/34)
Invitrogen
catalog: MA5-44046

rat monoclonal (YOL1/34)
reactivity: Saccharomyces cerevisiae S288C, human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

quantity: 100 ug
price: US 359.00
to the supplier

alpha Tubulin Monoclonal Antibody (A8-6)
Invitrogen
catalog: MA5-44621

mouse monoclonal (A8-6)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 470.00
to the supplier

Acetyl-alpha Tubulin (Lys40) Recombinant Rabbit Monoclonal Antibody (PSH0-13)
Invitrogen
catalog: MA5-50777

domestic rabbit recombinant (PSH0-13)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry

quantity: 100 uL
price: US 470.00
to the supplier

alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-116086

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

quantity: 100 uL
price: US 464.00
to the supplier

alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-116087

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

quantity: 100 uL
price: US 464.00
to the supplier

alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-143840

chicken polyclonal
reactivity: human, mouse, rat
application: western blot

quantity: 100 ug
price: US 424.00
to the supplier